WO2007112566A1 - Compositions et procédés de traitement de l'athérosclérose - Google Patents

Compositions et procédés de traitement de l'athérosclérose Download PDF

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WO2007112566A1
WO2007112566A1 PCT/CA2007/000528 CA2007000528W WO2007112566A1 WO 2007112566 A1 WO2007112566 A1 WO 2007112566A1 CA 2007000528 W CA2007000528 W CA 2007000528W WO 2007112566 A1 WO2007112566 A1 WO 2007112566A1
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seq
amino acid
cholesterol
mimetic
peptide
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PCT/CA2007/000528
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English (en)
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Perry M. Kim
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Queen's University At Kingston
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Priority to CA002645245A priority Critical patent/CA2645245A1/fr
Publication of WO2007112566A1 publication Critical patent/WO2007112566A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4711—Alzheimer's disease; Amyloid plaque core protein
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Definitions

  • the present invention relates to synthetic reverse peptides of a macrophage cholesterol ester hydrolase enhancing domain of mammalian serum amyloid A isoforms 2.1 (SAA2.1) and 1.1 (SAAl.1) or a portion thereof.
  • These reverse peptides, mimetics thereof or compositions containing these reverse peptides or mimetics thereof, and pharmaceutical compositions comprising one or more of these reverse peptides, mimetics thereof or compositions are useful in inhibiting the storage of cholesterol and potentiating the mobilization and release of cholesterol from inflammatory or atherosclerotic sites in a subject.
  • the reverse peptides and mimetics thereof, and the compositions and pharmaceutical compositions thereof are useful in the treatment and/or prevention of atherosclerosis and inflammation, as well as coronary heart disease and cardiovascular disease.
  • a reverse L amino acid and a retro- inversal D amino acid peptide having similar activity to its native forward L amino acid or D amino acid peptide are disclosed by Guo et al. (J. Pept. Res. 1997 50 (3) : 210-221 and J. Immunol 2002 169:2180-2188) and Shafiee et al. (Invest. Ophthalmol. Vis. Sci. 2000 41: 2378-2388).
  • Guo et al.(J. Pept. Res. 1997 50:210-221) showed that a retro-inverso peptide had similar anti-tumor activity to the native forward peptide.
  • Shafiee et al. Invest. Ophthalmol. Vis. Sci. 2000, 41: 2378-2388
  • certain D amino acid peptides were approximately two times more potent than their corresponding forward L amino acid peptides at inhibiting retinal angiogenesis in a retinal explant assay.
  • reverse peptides do not always exhibit similar efficacy to their corresponding forward peptide. Nor do they necessarily bind to the same catalytic or receptor site within or on an enzyme or protein as their corresponding forward peptide.
  • Zhou et al . JBC 2002 277:17476-17485) showed that a retro-inversal D amino acid peptide, which corresponds to a protein located on the HIV virus, binds to the CXCR4 chemokine receptor, but acts as an antagonist to the natural HIV protein ligand.
  • Buchet et al . Biochem. Biophys .
  • a selected peptide domain of mammalian serum amyloid A isoforms 2.1 (SAA2.1) and 1.1 (SAAl.1) and portions thereof and mimetics thereof have been demonstrated to have a potent enhancing effect on macrophage cholesterol ester hydrolase (CEH) activity.
  • CEH macrophage cholesterol ester hydrolase
  • reverse peptides of this domain, or a portion thereof also have a potent enhancing effect on CEH activity.
  • Reverse peptides of this domain or a portion thereof shift macrophage cholesterol into a transportable form that is then rapidly exported from the cell in the presence of a cholesterol transporter and a cholesterol acceptor, high density lipoprotein (HDL) .
  • HDL high density lipoprotein
  • these reverse peptides and mimetics thereof are useful in methods of inhibiting the storage of cholesterol and potentiating the mobilization and release of cholesterol from inflammatory or atherosclerotic sites in a subject.
  • reverse peptides and mimetics thereof are effective at regressing and/or decreasing formation of arterial atherosclerotic lesions and treating or preventing atherosclerosis, cardiovascular disease, coronary heart disease and inflammation in a subject .
  • the present invention provides reverse peptides of the CEH enhancing domain of SAA2.1 or SAAl .1 or a portion thereof, mimetics of these reverse peptides, and compositions comprising these reverse peptides or mimetics thereof, as well as pharmaceutical compositions comprising these reverse peptides, mimetics thereof, or compositions, and methods for use of these reverse peptides, mimetics thereof, compositions, and pharmaceutical compositions to modulate the activity of the macrophage cholesterol metabolizing enzyme CEH.
  • One aspect of the present invention relates to a reverse peptide, a peptide variant, or a mimetic thereof of the CEH enhancing domain of SAA proteins or a portion thereof.
  • CEH enhancing domains have been identified as residing in residues 74-103 of the C-terminus of murine SAA2.1, residues 77-103 of the C-terminus of murine SAAl.1, and 78-104 of the C-terminus of human SAAl .1 and SAA2.1.
  • Preferred reverse peptides or mimetics thereof capable of enhancing cholesterol ester hydrolase activity include a synthetic peptide or a mimetic thereof comprising a formula Xi 8 Xi7Xi 6 Xi5Xi4Xi3Xi 2 XiiXioX9X8X7X6X5X4X3X2Xi (SEQ ID NO : 1 ) or a portion thereof wherein Xi and X 9 , Xi 2 or Xi 8 are amino acids capable of forming a salt bridge, Xs is glutamic acid or lysine or an amino acid which is a conservative substitution thereof, and X 2 , X3, X4, X5, X7, X ⁇ / X10/ Xiir Xi3 > Xi 4/ Xi5 / Xi6 / and X 1 7 are independently any amino acid.
  • RFHNPDKGSRGWENAAQDA SEQ ID NO: 2 or a portion thereof. More preferred is a retro-inversal D amino acid peptide comprising RFHNPDKGSRGWENAAQDA (D-form; SEQ ID N0:3). These reverse peptides were derived from the forward human SAA 1.1 peptide ADQAANEWGRSGKDPNHFR (D-form; SEQ ID NOrIl).
  • Additional exemplary reverse peptides expected to enhance CEH activity based upon their similarity to SEQ ID NO: 2 and SEQ ID NO: 3 include, but are not limited to, reverse peptides comprising YKDPLGPPRYYNPDKGSRGHRNAEQDAITD(SEQ ID N0:4); YKDPLGPPRYYNPDKGSRGHRNAEQDA(SEQ ID NO:5); YKAPLGPPRYYNPDKGSRGHRNAEQDA(SEQ ID NO: 6); YKEPLGAPRFHNPDKGSRGWENAAQDA(SEQ ID NO:7); RYYNPDKGSRGHRNAEQDA (SEQ ID NO: 8); RFHNPDRGSRGWKNAAQDA (SEQ ID NO: 9); or RFHNPDRGSRGWKNAAQD(SEQ ID NO: 10) or portions thereof.
  • Preferred reverse peptide variants include, but are not limited to, cyclic reverse peptides and retro-inversal D amino acid peptides comprising one or more D amino acids, which are at least equally effective and superior in some embodiments but less susceptible to degradation in vivo than corresponding L amino acid peptides. More preferred is a retro-inversal D amino acid peptide variant comprising all D amino acids.
  • the present invention also relates to mimetics of any of the above reverse peptides, reverse peptide variants or portions thereof.
  • compositions with a formula of Y-Z or Q-Y-Z wherein Y comprises a reverse peptide or mimetic thereof of the present invention with CEH enhancing activity; Z comprises a compound linked to Y that enhances the performance of Y; and in embodiments comprising Q, Q comprises another compound linked to Y-Z which enhances performance of the Q- Y-Z composition.
  • Q may be identical to Z or different from Z.
  • Exemplary Z or Q compounds include, but are not limited to a targeting agent, a second agent for treatment of atherosclerosis, cardiovascular disease or coronary heart disease, an agent which enhances solubility, absorption, distribution, half-life, bioavailability, stability, activity and/or efficacy, and an agent which reduces toxicity or side effects of the composition.
  • Exemplary targeting agents of Z and/or Q include macrophage targeting agents such as, for example, a liposome, a microsphere, a ligand for a SAA receptor, hepatic targeting agents, antibodies and active fragments thereof such as, for example, Fab fragments, and additional agents specific to atherosclerotic plaques and/or inflammatory sites.
  • compositions comprising a reverse peptide, peptide variant, a mimetic thereof, or a Y-Z or Q-Y-Z composition which enhances CEH activity.
  • Pharmaceutical compositions of the present invention further comprise a vehicle that is pharmaceutically suitable for in vivo administration.
  • the reverse peptide, mimetic thereof or the composition is complexed with a lipid.
  • a phospholipid vesicle which encapsulates the reverse peptide, the mimetic thereof or the composition can also be used.
  • vehicles suitable for pharmaceutical administration in vivo may comprise aqueous solutions including, but not limited to, phosphate buffered saline or phosphate buffered saline containing glucose, preferably 0.01 to 10% weight/volume glucose, more preferably 5% weight/volume glucose.
  • Another aspect of the present invention relates to the use of these reverse peptides, mimetics thereof, compositions, or pharmaceutical compositions comprising these reverse peptides, mimetics thereof or compositions to modulate an activity of a cholesterol-metabolizing enzyme.
  • the activity of CEH can be modulated using a reverse peptide, a mimetic thereof, a composition, or a pharmaceutical composition comprising a reverse peptide, a mimetic thereof, or a composition of the present invention.
  • the enzymatic activity is modulated in vivo. More preferred is modulation of the enzymatic activity in humans. Even more preferred is that the CEH activity be enhanced.
  • Another aspect of the present invention relates to use of these reverse peptides, mimetics thereof, compositions, or pharmaceutical compositions comprising these reverse peptides, mimetics thereof, or compositions to increase and/or promote the mobilization and efflux of stored cholesterol from macrophages and other cells located in atherosclerotic plaques.
  • the increase and/or promotion of the mobilization and efflux of stored cholesterol from macrophages and other cells located in atherosclerotic plaques occurs in vivo. More preferred is an increase and/or promotion of the mobilization and efflux of stored cholesterol from macrophages and other cells located in atherosclerotic plaques in humans.
  • the reverse peptides, mimetics thereof, compositions and pharmaceutical compositions are also useful in modulating cholesterol metabolism in vivo in other cells including but not limited to hepatocytes, endothelial cells and epithelial cells.
  • Another aspect of the present invention relates to use of these reverse peptides, mimetics thereof, compositions or pharmaceutical compositions comprising these reverse peptides, mimetics thereof or compositions to increase and/or promote the mobilization and efflux of stored cholesterol from macrophages and other cells located at sites of inflammation.
  • the increase and/or promotion of the mobilization and efflux of stored cholesterol from macrophages and other cells located at sites of inflammation occurs in vivo. More preferred is an increase and/or promotion of the mobilization and efflux of stored cholesterol from macrophages and other cells located at sites of inflammation in humans.
  • Another aspect of the present invention relates to methods for treating and/or preventing atherosclerosis and/or regressing or decreasing formation of arterial atherosclerotic lesions in a subject comprising administering to the subject a reverse peptide, a mimetic thereof, a composition or a pharmaceutical composition of the present invention.
  • the subject is a human.
  • Another aspect of the present invention relates to methods for treatment of cardiovascular disease comprising administering to a subject a reverse peptide, a mimetic thereof, a composition or a pharmaceutical composition of the present invention.
  • the subject is a human.
  • Another aspect of the present invention relates to methods for treatment of coronary heart disease comprising administering to a subject a reverse peptide or a mimetic thereof, a composition or a pharmaceutical composition of the present invention.
  • the subject is a human.
  • Yet another aspect of the present invention relates to methods for treating and/or preventing or inhibiting inflammation in a subject comprising administering to the subject a reverse peptide or a mimetic thereof, a composition or a pharmaceutical composition of the present invention.
  • the subject is a human.
  • Figure 1 is a bar graph comparing cholesterol efflux in an in vivo cholesterol export study in mice administered by tail vein injection retro-inversal D amino acid peptide RFHNPDKGSRGWENAAQDA (SEQ ID NO: 3) at a total dose of 300 ⁇ g in 100 ⁇ l 0.9% saline versus control mice wherein vehicle (100 ⁇ l 0.9% saline) was administered by tail vein injection.
  • statins are for the most part considered safe and highly effective.
  • acyl CoA cholesterol acyl transferase
  • D-4F D amino acid peptide sequence within the ApoA- 1 apolipoprotein
  • the D-4F peptide which is 18 amino acids in length, has been shown to promote the reverse cholesterol transport (RCT) pathway and thus enhance cholesterol removal from atherosclerotic plaque (Navab et al. Circulation 2002 105:290-292; Navab et al.
  • Macrophages are key cells in the storage and removal of lipids. Their conversion to foam cells (cholesterol-laden macrophages) is an early and important pathological process in the formation of an atherosclerotic plaque. Macrophages and foam cells are used herein synonymously.
  • CEH cholesterol ester hydrolase
  • AP-HDL acute phase-HDL
  • HDL- SAA acute phase-HDL
  • liposomes containing murine SAA2.1 have been shown to cause a marked enhancement of CEH activity in intact cholesterol-laden macrophages in tissue culture.
  • HDL alone, SAA2.1-free liposomes, and liposomes containing murine SAAl .1 or apoA-1 had no effect.
  • the C-terminal 30-residue domain of murine SAA2.1 correlating to amino acids 74-103 of murine SAA2.1 was identified as the region of murine SAA2.1 that enhances CEH activity.
  • the CEH activity-enhancing domain has been identified as correlating to amino acids 77-95 of murine SAA2.1.
  • isolated peptides with amino acid sequences comprising this domain or a portion thereof within murine SAA2.1, human SAAl .1 and human SAA2.1 have been shown to have a potent enhancing effect on macrophage CEH activity both in vitro and in vivo.
  • reverse peptide as used herein, it is meant a peptide having an amino acid sequence wherein the amino acids are positioned in opposite order to the forward amino acid sequence, or a portion thereof, depicted, for example, in Tables 1 and 2.
  • Tables 1 and 2 depicted, for example, for full length murine SAAl .1
  • reverse peptide it is meant to be inclusive of reverse L amino acid peptides, reverse D amino acid peptides, sometimes referred to as retro-inversal (also known as retro-inverso, retro-enantio, retro-all) D amino acid peptides, as well as peptides comprising L amino acids and D amino acids .
  • This peptide is the reverse sequence of the forward human D amino acid 19-mer peptide ADQAANEWGRSGKDPNHFR (D-form; SEQ ID NO: 11) disclosed in U.S. Patent Application Serial No.
  • the retro-inversal D amino acid peptide RFHNPDKGSRGWENAAQDA (SEQ ID NO: 3) at a total dose of 300 ug, was injected into the tail vein of each mouse that was previously (24 hours earlier) injected with H 3 -radiolabeled cholesterol-loaded macrophages.
  • This reverse peptide was dissolved in 0.9% saline and administered at a volume of 100 ul per mouse, to achieve a final total dose of 300 ug per mouse.
  • Blood samples were collected 5 hours post reverse peptide injection. Approximately 25 ⁇ l of blood were collected from the tail vein of each animal. The blood samples were centrifuged to separate the red blood cells from the plasma and the [ 3 H] -cholesterol in plasma was determined by scintillation counting. Results are mean ;+ SEM of four determinations .
  • This increase in macrophage cholesterol export was over 2.5-fold higher than control animals treated with saline vehicle alone. Saline vehicle does not enhance cholesterol export.
  • retro-inversal D amino acid peptides of the present invention are capable of reaching distally-located cholesterol-loaded macrophages.
  • these reverse peptides are capable of targeting and entering cholesterol-loaded macrophages and promoting macrophage cholesterol export.
  • the experiments set forth herein demonstrate that synthetic reverse peptides of the SAA CEH enhancing domain or a portion thereof, markedly increased in vivo cholesterol efflux.
  • the data substantiate the utility of designing and using reverse peptides or mimetics thereof of the SAA CEH domain or a portion thereof to reduce or prevent atherogenesis and/or cause regression of an atherosclerotic plaque by increasing the efflux of cholesterol from macrophages located in an atherosclerotic lesion.
  • Such reverse peptides or mimetics thereof will be useful in the treatment or prevention of atherosclerosis and in the treatment of coronary heart disease and cardiovascular disease associated with atherosclerosis.
  • the present invention provides reverse peptides, mimetics thereof, Y-Z and Q-Y-Z compositions, and pharmaceutical compositions comprising a reverse peptide, a mimetic thereof or a Y-Z or Q-Y-Z composition, for use in the prevention and/or treatment of atherosclerosis as well as coronary heart disease and cardiovascular disease associated with atherosclerosis and/or in the regression or decrease in formation of arterial atherosclerotic lesions.
  • the reverse peptide or mimetic thereof is a retro-inversal D amino acid peptide or mimetics thereof of the present invention containing one or more D amino acids.
  • compositions of the present invention comprise a reverse peptide of the SAA CEH enhancing domain of SAA2.1 or a portion thereof or a mimetic thereof, or a Y-Z or Q-Y-Z composition.
  • portion thereof it is meant to be inclusive of reverse peptides exhibiting similar biological activities to the reverse peptides described herein but which, (1) comprise shorter fragments of the reverse sequence of the CEH enhancing domains of murine SAA2.1, murine SAAl.1, human SAAl .1 or human SAA2.1 described herein or (2) overlap with only part of the reverse sequence of the cholesterol enhancing domains of murine SAA2.1, murine SAAl.1, human SAAl .1 or human SAA2.1 described herein.
  • synthetic as used herein it is meant that the peptide is prepared synthetically, either by chemical means or recombinantly .
  • Preferred reverse peptides capable of enhancing CEH activity for use in the present invention comprise a synthetic peptide or a mimetic thereof comprising a formula X18X17X16X15X14X13X12X11X10X9X8X7X6X5X4X3X2X1 (SEQ ID N0:l) or a portion thereof wherein Xi and X 9 , X i2 or Xi 8 are amino acids capable of forming a salt bridge, X 6 is glutamic acid or lysine or an amino acid which is a conservative substitution thereof, and X 2 , X3, X4, X5, X7, Xs, X10, Xn, X13, X14, X15, Xi6, and X i7 are independently any amino acid.
  • Xi and X 9 , Xi 2 or Xi 8 are amino acids capable of forming a salt bridge
  • X 2 is glutamine or an amino acid which is a conservative substitution thereof
  • X 3 and X 4 are independently alanine or an amino acid which is a conservative substitution thereof
  • X 5 and Xi5 are independently asparagine or an amino acid which is a conservative substitution thereof
  • X 7 is tryptophan or an amino acid which is a conservative substitution thereof
  • X 8 and Xn are independently glycine or an amino acid which is a conservative substitution thereof
  • X 10 is serine or an amino acid which is a conservative substitution thereof
  • X i3 is aspartic acid or an amino acid which is a conservative substitution thereof
  • X i4 is proline or an amino acid which is a conservative substitution thereof
  • Xii amino acids capable of forming a salt bridge
  • X 2 is glutamine or an amino acid which is a conservative substitution thereof
  • amino acid combinations capable of forming a salt bridge include Xi being an aspartic acid and Xg, X 12 or Xi 8 being an arginine . It is preferred that the reverse peptide or mimetic thereof have equal to or less amino acid residues as compared to the full length forward SAA protein, more preferably less than 80 amino acid residues, more preferably less than 50 amino acids, more preferably less than 35, 30, or 25 amino acids and most preferably 20 or less, or 18 or less. As will be understood by the skilled artisan upon reading the disclosure, the minimal length of a reverse peptide of the present invention will be the lowest number of amino acids in sequence which maintain CEH enhancing properties while providing for cost effective production and/or minimizing degradation.
  • the reverse peptide or mimetic thereof comprises one or more D amino acids.
  • a reverse peptide comprising RFHNPDKGSRGWENAAQDA (SEQ ID NO: 2) or a portion thereof. More preferred is a retro-inversal D amino acid peptide comprising RFHNPDKGSRGWENAAQDA (D-form; SEQ ID NO:3).
  • Additional exemplary reverse peptides expected to enhance CEH activity based upon their similarity to SEQ ID NO: 2 and SEQ ID NO: 3 include, but are not limited to, reverse peptides comprising YKDPLGPPRYYNPDKGSRGHRNAEQDAITD(SEQ ID NO:4); YKDPLGPPRYYNPDKGSRGHRNAEQDA(SEQ ID N0:5); YKAPLGPPRYYNPDKGSRGHRNAEQDA(SEQ ID NO: 6); YKEPLGAPRFHNPDKGSRGWENAAQDA(SEQ ID NO:7); RYYNPDKGSRGHRNAEQDA (SEQ ID NO: 8); RFHNPDRGSRGWKNAAQDA (SEQ ID NO: 9); or RFHNPDRGSRGWKNAAQD(SEQ ID NO: 10) or portions thereof.
  • the above exemplary peptides comprise one or more D amino acids.
  • compositions with a formula of Y-Z or Q-Y-Z are also preferred for use in the present invention to enhance CEH activity.
  • Z is linked to Y and/or Q is linked to Y-Z via any acceptable binding means and selected based upon selection of Z or Q.
  • acceptable binding means include, but are in no way limited to, covalent binding, noncovalent binding, hydrogen binding, antibody-antigen recognition, and ligand binding.
  • Y comprises a reverse peptide or mimetic thereof of the present invention with CEH enhancing activity
  • Z comprises a compound linked to Y that enhances the performance of Y
  • Q may be identical to Z or different from Z and also enhances performance of the composition Q-Y-Z.
  • Preferred are compositions with retro- inversal D amino acid peptides or mimetics thereof containing one or more D amino acids.
  • Exemplary Z or Q compounds include, but are not limited to, a targeting agent, a second agent for treatment of atherosclerosis, cardiovascular disease or coronary heart disease, an agent which enhances solubility, absorption, distribution, half- life, bioavailability, stability, activity and/or efficacy, and an agent which reduces toxicity or side effects of the composition.
  • Exemplary targeting agents of Z and/or Q include macrophage targeting agents such as, for example, a liposome, a microsphere, a ligand for a SAA receptor, hepatic targeting agents, antibodies and active fragments thereof such as, for example, Fab fragments, and additional agents specific to atherosclerotic plaques and/or inflammatory sites.
  • human equivalent as used herein, it is meant a reverse peptide sequence derived from human SAA2.1 or human SAAl.1 with similar activity to the murine peptides referenced herein.
  • mimetic as used herein it is meant to be inclusive of reverse peptides, which may be recombinant, and peptidomimetics, as well as small organic molecules, which exhibit similar or enhanced CEH modulating activity.
  • reverse peptide variants which comprise conservative amino acid substitutions relative to the reverse seguences of the native domains of SAA2.1 or SAAl .1
  • reverse peptide variants which have a high percentage of sequence identity with the native domains of the reverse sequences of SAA2.1 or SAAl.1, at least, e.g., 70%, 75%, 80%, 85%, 90%, preferably at least 95%, 96%, 97%, 98%, or 99% sequence identity, and more preferably at least 99.5% or 99.9% sequence identity.
  • Variant reverse peptides can be aligned with the reference reverse peptide sequence to assess percentage sequence identity in accordance with any of the well-known techniques for alignment. For example, a variant reverse peptide greater in length than a reference reverse peptide sequence is aligned with the reference reverse peptide sequence using any well known technique for alignment and percentage sequence identity is calculated over the length of the reference reverse peptide sequence, notwithstanding any additional amino acids of the variant reverse peptide, which may extend beyond the length of the reference reverse peptide sequence.
  • Preferred reverse variant peptides include, but are not limited to, retro-inversal D amino acid peptides comprising one or more D amino acids, which are also effective but generally less susceptible to degradation in vivo than corresponding L amino acid peptides, and cyclic peptides.
  • retro-inversal D amino acid peptides of the present invention are effective at increasing cholesterol efflux in vivo when administered intravenously in an aqueous vehicle. Like D forward peptides it is expected that the retro-inversal D amino acid peptides of the present invention will also be effective orally.
  • Lipid/liposome formulations of reverse peptides of the present invention are also expected to be effective at increasing cholesterol efflux.
  • the reverse peptides and mimetics thereof are therefore believed to be effective at regressing and/or decreasing formation of arterial atherosclerotic lesions and treating or preventing atherosclerosis, cardiovascular disease, coronary heart disease and inflammation in a subject.
  • Cyclic peptides can be circularized by various means including but not limited to peptide bonds or depsicyclic terminal residues (i.e. a disulfide bond).
  • peptidomimetic is intended to include analogs of peptides that serve as appropriate substitutes for the exemplary reverse peptides of SEQ ID NO: 2 through 10 in modulating CEH activity.
  • the peptidomimetic must possess not only similar chemical properties, e.g. affinity, to this peptide domain, but also efficacy and function. That is, a peptidomimetic exhibits function (s) of a CEH enhancing domain of SAA, without restriction of structure.
  • Peptidomimetics of the present invention i.e. analogs of the CEH enhancing domain of SAA2.1 or 1.1, include amino acid residues or other moieties which provide the functional characteristics described herein.
  • Mimetics of the present invention may be designed to have a similar structural shape to the CEH enhancing domain of SAA or a portion thereof.
  • mimetics according to the present invention of the CEH enhancing domain or a portion thereof may be designed to include a structure which mimics the salt bridge conformation of X18X17X16X15X14X13X12X11X10X9X8X7X6X5X4X3X2X1 (SEQ ID N0:l) or a portion thereof wherein Xi and Xg, X 12 or Xi ⁇ are amino acids capable of forming a salt bridge, X 6 is glutamic acid or lysine or an amino acid which is a conservative substitution thereof, and X2, X3, X4, X5, X7, Xs, X10/ Xii/ X13, Xi4r Xi5, Xi ⁇ , and X17 are independently any amino acid.
  • Mimetics of CEH enhancing domain of SAA or portion thereof can also be designed to have a similar structure to the synthetic reverse peptides of SEQ ID NO: 2 through 10.
  • These peptidomimetics may comprise peptide sequences with conservative amino acid substitutions as compared to SEQ ID NO: 2 through 10 which interact with surrounding amino acids to form a similar structure to these synthetic reverse peptides.
  • Conformationally restricted moieties such as a tetrahydroisoquinoline moiety may also be substituted for a phenylalanine, while histidine bioisoteres may be substituted for histidine to decrease first pass clearance by biliary excretion.
  • Peptidomimetics of the present invention may also comprise peptide backbone modifications.
  • Analogues containing amide bond surrogates are frequently used to study aspects of peptide structure and function including, but not limited to, rotational freedom in the backbone, intra- and intermolecular hydrogen bond patterns, modifications to local and total polarity and hydrophobicity, and oral bioavailability.
  • Mimetics can also be designed with extended and/or additional amino acid residue repeats as compared to the reverse sequence of the naturally occurring CEH enhancing domain of SAA or portion thereof. Identification of these reverse peptides also permits molecular modeling based on these reverse peptides for design, and subsequent synthesis, of small organic molecules that have CEH enhancing activities. These small organic molecules mimic the structure and activity of the reverse peptides of SEQ ID NO: 1 through 10. However, instead of comprising amino acids, these small organic molecules comprise bioisosteres thereof, substituents or groups that have chemical or physical similarities, and exhibit broadly similar biological activities.
  • Bioisosterism is a lead modification approach used by those skilled in the art of drug design and shown to be useful in attenuating toxicity and modifying activity of a lead compound such as SEQ ID NO:1, 2, 3, 4, 5, 6, 7, 8, 9, or 10.
  • Bioisosteric approaches are discussed in detail in standard reference texts such as The Organic Chemistry of Drug Design and Drug Action (Silverman, RB, Academic Press, Inc. 1992 San Diego, CA, pages 19-23) .
  • Classical bioisosteres comprise chemical groups with the same number of valence electrons but which may have a different number of atoms.
  • classical bioisosteres with univalent atoms and groups include, but are not limited to: CH 3 , NH 2 , OH, F and Cl; Cl, PH 2 and SH; Br and i-Pr; and I and t-Bu.
  • Classical bioisosteres with bivalent atoms and groups include, but are not limited to: -CH 2 - and NH; 0, S, and Se; and COCH 2 , CONHR, CO 2 R and COSR.
  • Classical bioisosteres with ring equivalents include, but are not limited to: benzene and thiophene; benzene and pyridine; and tetrahydrofuran, tetrahydrothiophene, cyclopentane and pyrrolidine.
  • Nonclassical bioisosteres still produce a similar biological activity, but do not have the same number of atoms and do not fit the electronic and steric rules of classical isosteres. Exemplary nonclassical bioisoteres are shown in the following Table.
  • Additional bioisosteric interchanges useful in the design of small organic molecule mimetics of the present invention include ring-chain transformations.
  • a reverse peptide, a mimetic thereof or a Y-Z or Q-Y-Z composition of the present invention is preferably formulated with a vehicle pharmaceutically acceptable for administration to a subject, preferably a human, in need thereof.
  • vehicle pharmaceutically acceptable for administration preferably a human
  • Methods of formulation for such compositions are well known in the art and taught in standard reference texts such as Remington 's Pharmaceutical Sciences, Mack Publishing Co., Easton, PA, 1985.
  • a composition of the present invention may comprise a single reverse peptide, a mimetic thereof or a Y-Z or Q-Y-Z composition which modulates CEH activity.
  • compositions of the present invention may be administered alone or in combination with a second cholesterol-lowering drug or agent.
  • a composition of the present invention comprising a reverse peptide of SEQ ID N0:l, 2, 3, 4, ,5 ,6 ,7 ,8, 9, or 10 or a mimetic thereof which inhibits CEH activity, can be administered to a subject in combination with an ACAT inhibitor.
  • ACAT inhibitors include but are not limited to avasimibe (Pfizer) , eflucimibe (Eli Lilly) and pactimibe (Sankyo) .
  • compositions of the present invention may also be administered to a subject with an apolipoprotein free cholesterol acceptor (Rothblat et al. J. Lipid Res. 1999 40:781-796; Li et al . Biochimica Biophysica Acta 1995 1259:227-234; Jian et al . J. Biol. Chem. 1998 273 ( 10 ): 5599-5606) .
  • An example of an apolipoprotein free cholesterol acceptor is cyclodextrin.
  • Additional exemplary cholesterol-lowering drugs or agents which can be administered in combination with a reverse peptide, mimetic thereof or composition of the present invention include, but are not limited to, statins, resins, bile acid sequestrants (Bays et al. Expert Opinion on Pharmacotherapy 2003 4 (11) : 1901-38; Kajinami et al. Expert Opinion on Investigational Drugs 2001 11 ( 6) : 831-5) , niacin (Van et al. Am. J. Cardiol. 2002 89 (11) : 1306-8; Ganji et al. J. Nutri. Biochem. 2003 14 ( 6) : 298-305; Robinson et al. Progress in Cardiovasc.
  • a preferred formulation for use in the present invention is complexing the reverse peptide, mimetic thereof or Y-Z or Q-Y-Z composition with a lipid.
  • a formulation is encapsulation of the reverse peptide, mimetic thereof or Y-Z or Q-Y-Z composition in a phospholipid vesicle.
  • An exemplary phospholipid vesicle useful in the present invention is a liposome. Liposomes containing the reverse peptide, mimetic thereof or Y-Z or Q-Y-Z composition of the present invention can be prepared in accordance with any of the well known methods such as described by Epstein et al . (Proc. Natl. Acad. Sci. USA 82: 3688-3692 (1985)), Hwang et al. (Proc. Natl. Acad. Sci.
  • Preferred liposomes are of the small (about 200-800 Angstroms) unilamellar type in which the lipid content is greater than about 10 mol. percent cholesterol, preferably in a range of 10 to 40 mol. percent cholesterol, the selected proportion being adjusted for optimal peptide therapy.
  • phospholipid vesicles other than liposomes can also be used.
  • another preferred formulation contains a reverse peptide, more preferably a retro- inversal D amino acid peptide comprising one or more D amino acids and an aqueous vehicle suitable for intravenous or oral administration.
  • Formulations expected to be useful in the present invention include, but are not limited to, sterile aqueous solutions (where water soluble) or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions.
  • the composition must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi.
  • the vehicle can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, liquid polyethylene glycol, and the like), suitable mixtures thereof, and oils (e . g ., vegetable oil).
  • polyol for example, glycerol, propylene glycol, liquid polyethylene glycol, and the like
  • oils e . g ., vegetable oil.
  • the proper fluidity can be maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersion, and by the use of surfactants.
  • Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like.
  • isotonic agents for example, sugars, sodium chloride, or polyalcohols such as mannitol and sorbitol, in the composition.
  • Prolonged absorption of the injectable compositions can be brought about by including in the composition an agent which delays absorption, for example, aluminum monostearate or gelatin.
  • Sterile injectable solutions can be prepared by incorporating the reverse peptide of the present invention in the required amount in an appropriate solvent with one or a combination of ingredients enumerated above, as required, followed by filter sterilization.
  • dispersions are prepared by incorporating the reverse peptide of the present invention into a sterile vehicle which contains a basic dispersion medium and the required other ingredients from those enumerated above.
  • the preferred methods of preparation are vacuum drying and freeze-drying which yield a powder of the active ingredient (i.e., the peptide) optionally plus any additional desired ingredient from a previously sterile-filtered solution thereof.
  • Solid dosage forms for oral administration of a reverse peptide of the present invention include, but are not limited to, ingestible capsules, tablets, pills, lollipops, powders, granules, elixirs, suspensions, syrups, wafers, sublingual or buccal tablets, troches, and the like.
  • the reverse peptide is mixed with at least one inert, pharmaceutically acceptable excipient or diluent or assimilable edible carrier such as sodium citrate or dicalcium phosphate and/or a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar- agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h)
  • the dosage form may also comprise buffering agents.
  • Solid compositions of a similar type may also be employed as fillers in soft and hard- filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.
  • the percentage of the reverse peptide in the compositions and preparations may, of course, be varied. The amount of the reverse peptide in such therapeutically useful compositions is such that a suitable dosage will be obtained.
  • the solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well- known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient (s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions which can be used include polymeric substances and waxes. The reverse peptides can also be in micro-encapsulated form, if appropriate, with one or more of the above-mentioned excipients .
  • Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, solutions, suspensions, syrups and elixirs.
  • the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethyl formamide, oils (in particular, cottonseed, ground nut corn, germ olive, castor, and sesame oils) , glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof.
  • the oral compositions can also include adjuvants such as wetting agents, emulsifying
  • Suspensions in addition to the reverse peptide, may contain suspending agents as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, and tragacanth, and mixtures thereof.
  • suspending agents as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, and tragacanth, and mixtures thereof.
  • the reverse peptides, mimetics thereof, compositions or pharmaceutical compositions of the present invention can also be administered via a coronary stent implanted into a patient.
  • Coronary stents which elute a reverse peptide, a mimetic thereof, a composition or a pharmaceutical composition of the present invention can be prepared and implanted in accordance with well known techniques (See, for example, Woods et al. (2004) Annu . Rev. Med. 55:169- 78); al-Lamce et al . (2003) Med. Device Technol . 2003 14:12-141 Lewis et al. 2002 J. Long Term Eff. Med. Implants 12:231-50; Tsuji et al. 2003 Int. J. Cardiovasc.
  • compositions of the present invention are useful in modulating the activity of a cholesterol- metabolizing enzyme, and in particular, the activity of CEH.
  • the pharmaceutical compositions are used to modulate enzymatic activity in macrophages. More preferably, the pharmaceutical compositions are used to modulate enzymatic activity in vivo. More preferably, the pharmaceutical compositions are used to modulate enzymatic activity in mammals and in particular humans. More preferably, the pharmaceutical compositions are used to enhance CEH activity in mammals and in particular humans.
  • compositions of the present invention are also useful in promoting the mobilization and efflux of stored cholesterol located in atherosclerotic plaques and/or sites of inflammation.
  • the pharmaceutical compositions are used to promote the mobilization and efflux of stored cholesterol from macrophages and other cells (e.g. hepatocytes, smooth muscle cells, endothelial cells and epithelial cells), including cells and tissues located in atherosclerotic plaques or sites of inflammation in vivo. More preferably, the pharmaceutical compositions are used to promoting the mobilization and efflux of stored cholesterol from macrophages and other cells and tissues located in atherosclerotic plaques or sites of inflammation in mammals and in particular humans.
  • macrophages and other cells e.g. hepatocytes, smooth muscle cells, endothelial cells and epithelial cells
  • the pharmaceutical compositions are used to promoting the mobilization and efflux of stored cholesterol from macrophages and other cells and tissues located in atherosclerotic plaques or sites of inflammation in mammals and in particular
  • compositions of the present invention can be administered to a subject, preferably a mammal, more preferably a human, to treat and/or prevent atherosclerosis.
  • the compositions may be administered by various routes including, but not limited to, orally, intravenously, intramuscularly, intraperitoneally, topically, subcutaneously, rectally, dermally, sublingually, buccally, intranasally or via inhalation.
  • the composition administered comprises a retro-inversal D amino acid peptide or mimetic thereof with one or more D amino acids.
  • the formulation and route of administration as well as the dose and frequency of administration can be selected routinely by those skilled in the art based upon the severity of the condition being treated, as well as patient-specific factors such as age, weight and the like.
  • the prolonged activity of forward peptides of this domain in promoting cholesterol efflux from macrophages is indicative of the feasibility of daily, every other day or semi-weekly dosing regime for the pharmaceutical compositions comprising a reverse peptide, mimetic thereof, or composition of the present invention as well.
  • efficacy of reverse peptides, mimetics thereof and compositions of the present invention to treat and/or prevent atherosclerosis can also be demonstrated in an animal model such as the apoE knockout mouse model of atherogenesis (Davis et al . Arterioscler Thromb Vase Biol. 2001 21:2031-2038). These mice, when placed on an atherogenic diet (as described by Tarn et al . J. Lipid Res. 2005 46:2091-2101), rapidly deposit lipid into their aortas.
  • the apoE knockout mice are a validated model of atherosclerosis and were used to demonstrate the effectiveness of ezetimibe (ZetiaTM; Merck) in reducing atherosclerosis (Davis et al . Arterioscler Thromb Vase Biol. 2001 21:2031-2038).
  • the efficacy of reverse peptides, mimetics thereof and compositions of the present invention such as, e.g., those comprising one or more reverse peptides of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 or a mimetic thereof, in treating or preventing atherosclerosis can be demonstrated in similar fashion.
  • a reverse peptide, mimetic thereof or composition of the present invention such as one comprising a reverse peptide of SEQ ID NO:1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 in preventing or reducing the degree of atherosclerosis
  • the rodent is first placed on an atherogenic diet for two weeks. The animals are then divided into three groups. The first group of animals serves as the baseline group.
  • the second and third groups of animals are sacrificed just prior to treatment of the second and third groups of animals and the aortas are analyzed as described below.
  • the second group of animals continues on the diet for an additional two weeks and is treated with vehicle only.
  • the third group of animals continues on the diet for the same period but also receives a reverse peptide, mimetic thereof or composition of the present invention.
  • the effects of a reverse peptide, mimetic thereof or composition of the present invention on aortic atherosclerosis are assessed at the termination of the experiment, when the aorta is removed from the animals and opened longitudinally.
  • Peptides were synthesized by solid-phase chemistry using a PE Applied Biosystems 433A peptide synthesizer. The purity of the synthetic peptides was established by analytical high-performance liquid chromatography (HPLC) and ion spray mass spectrometry. The peptides were dialyzed against distilled water and lyophilized before use.
  • HPLC high-performance liquid chromatography
  • ion spray mass spectrometry The peptides were dialyzed against distilled water and lyophilized before use.
  • J774 cells cultured in 6-well plates were enriched with cholesterol by incubating with red blood cell (RBC) membrane fragments (175 ⁇ g as cholesterol) that had been previously labeled with 0.5 ⁇ Ci/ml [ 3 H] -cholesterol at 37 0 C for 24 hours in 0.2% bovine serum albumin (BSA) . After loading with the labelled RBC membrane for 6 hours, cells were then washed with phosphate-buffered saline/BSA
  • Baseline cholesterol efflux was determined by measuring the amount of [ 3 H] -cholesterol in plasma by scintillation spectrometry.
  • D-form SEQ ID NO:3
  • SEQ ID NO:3 24 hours after injection of [ 3 H] -cholesterol-loaded J774 macrophages into mice and after the first blood sample was collected, the same animals received non-liposome formulated retro- inversal peptide RFHNPDKGSRGWENAAQDA (D-form; SEQ ID NO: 3) at a total dose of 300 ⁇ g dissolved in 100 ⁇ l of PBS.
  • the peptide was injected into the tail vein of each animal. Five hours following intravenous injection of the reverse peptide, approximately 25 ⁇ l of blood were collected from the tail vein of each animal into heparinized capillary tubes and cholesterol efflux was determined as described above. Data from this study are set forth in Figure 1.

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Abstract

L'invention porte sur des peptides inverses et leurs mimétiques d'un domaine de l'isoforme A de la protéine amyloïde sérique mammalienne 2.1 (SAA2.1) ou une partie de celle-ci, ses compositions et compositions pharmaceutiques qui permettent de renforcer l'effet sur l'activité de la cholestérol ester hydrolase des macrophages. L'invention concerne des procédés d'utilisation de ces peptides inverses, de leurs mimétiques et compositions dans le traitement et/ou la prévention de l'athérosclérose, de la maladie coronarienne et de la maladie cardio-vasculaire.
PCT/CA2007/000528 2006-03-31 2007-03-30 Compositions et procédés de traitement de l'athérosclérose WO2007112566A1 (fr)

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