WO2007078599A8 - Functional arrays for high throughput characterization of gene expression regulatory elements - Google Patents
Functional arrays for high throughput characterization of gene expression regulatory elementsInfo
- Publication number
- WO2007078599A8 WO2007078599A8 PCT/US2006/046920 US2006046920W WO2007078599A8 WO 2007078599 A8 WO2007078599 A8 WO 2007078599A8 US 2006046920 W US2006046920 W US 2006046920W WO 2007078599 A8 WO2007078599 A8 WO 2007078599A8
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- regulatory elements
- gene expression
- high throughput
- expression regulatory
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
Abstract
The present invention provides compositions, kits, assemblies, libraries, arrays, and high throughput methods for large scale structural and functional characterization of gene expression regulatory elements in a genome of an organism, especially in a human genome. In one aspect of the invention, an array of expression constructs is provided, e.ach of the expression constructs comprising: a nucleic acid segment operably linked with a reporter sequence in an expression vector such that expression of the reporter sequence is under the transcriptional control of the nucleic acid segment, the nucleic acid segment varying in the library and having a diversity of at least 50. The nucleic acid segments can be a large library of gene expression regulatory elements such as transcriptional promoters. The present invention can have a wide variety of applications such as in personalized medicine, pharmacogenomics, and correlation of polymorphisms with phenotypic traits.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008545677A JP2009519710A (en) | 2005-12-16 | 2006-12-08 | Functional arrays for high-throughput characterization of gene expression regulatory elements |
EP06849046A EP2021499A4 (en) | 2005-12-16 | 2006-12-08 | Functional arrays for high throughput characterization of gene expression regulatory elements |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US75092905P | 2005-12-16 | 2005-12-16 | |
US60/750,929 | 2005-12-16 | ||
US76205606P | 2006-01-24 | 2006-01-24 | |
US60/762,056 | 2006-01-24 | ||
US11/636,385 US20070161031A1 (en) | 2005-12-16 | 2006-12-07 | Functional arrays for high throughput characterization of gene expression regulatory elements |
US11/636,385 | 2006-12-07 |
Publications (4)
Publication Number | Publication Date |
---|---|
WO2007078599A2 WO2007078599A2 (en) | 2007-07-12 |
WO2007078599A9 WO2007078599A9 (en) | 2007-10-04 |
WO2007078599A3 WO2007078599A3 (en) | 2008-08-28 |
WO2007078599A8 true WO2007078599A8 (en) | 2008-10-30 |
Family
ID=38228711
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2006/046920 WO2007078599A2 (en) | 2005-12-16 | 2006-12-08 | Functional arrays for high throughput characterization of gene expression regulatory elements |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP2021499A4 (en) |
JP (1) | JP2009519710A (en) |
WO (1) | WO2007078599A2 (en) |
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AU2012204266C1 (en) * | 2011-01-07 | 2017-12-21 | Applied Genetic Technologies Corporation | Promoters, expression cassettes, vectors, kits, and methods for the treatment of achromatopsia and other diseases |
US9557327B2 (en) | 2012-04-03 | 2017-01-31 | National Center For Child Health And Development | DNA controlling miR-140 expression, and screening method of drugs using said DNA |
US9163259B2 (en) | 2012-05-04 | 2015-10-20 | Novartis Ag | Viral vectors for the treatment of retinal dystrophy |
WO2014031079A1 (en) * | 2012-08-21 | 2014-02-27 | Singapore Health Services Pte Ltd | Method and/or probe for determining glaucoma susceptibility |
KR102495527B1 (en) | 2013-03-14 | 2023-02-06 | 몬산토 테크놀로지 엘엘씨 | Plant regulatory elements and uses thereof |
WO2014197586A1 (en) | 2013-06-04 | 2014-12-11 | Virginia Commonwealth University | Mda-9/syntenin promoter to image and treat metastatic cancer cells |
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US20150276767A1 (en) * | 2014-03-12 | 2015-10-01 | The University Of Sydney | Systems and methods for identifying cancers having activated progesterone receptors |
WO2015143385A1 (en) * | 2014-03-21 | 2015-09-24 | Life Technologies Corporation | Multi-copy reference assay |
CN103981185B (en) * | 2014-04-14 | 2016-04-27 | 浙江理工大学 | Liver cancer-specific GP73 core promoter and screening construction process thereof |
KR101791296B1 (en) | 2014-04-17 | 2017-10-27 | 제주대학교 산학협력단 | Expression cassette and vector with genes related Alzheimer's disease and transgenic cell line made from it |
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WO2016149455A2 (en) * | 2015-03-17 | 2016-09-22 | The General Hospital Corporation | The rna interactome of polycomb repressive complex 1 (prc1) |
EP3307888A1 (en) * | 2015-06-12 | 2018-04-18 | Erasmus University Medical Center Rotterdam | New crispr assays |
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WO2018102369A1 (en) | 2016-11-30 | 2018-06-07 | Arno Therapeutics, Inc. | Methods for onapristone synthesis dehydration and deprotection |
WO2018111104A1 (en) * | 2016-12-14 | 2018-06-21 | Erasmus University Medical Center Rotterdam | Use of human crispr sequences in diagnostics |
WO2018178067A1 (en) * | 2017-03-27 | 2018-10-04 | Vrije Universiteit Brussel | Diaphragm-specific nucleic acid regulatory elements and methods and use thereof |
CN117821509A (en) | 2017-04-03 | 2024-04-05 | 编码治疗公司 | Tissue-selective transgene expression |
KR102625470B1 (en) | 2017-05-31 | 2024-01-16 | 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐 | Optimized human coagulation factor IX gene expression cassette and use thereof |
RU2671156C1 (en) * | 2017-08-21 | 2018-10-29 | Общество с ограниченной ответственностью "Центр Генетики и Репродуктивной Медицины "ГЕНЕТИКО" | Method of preimplantation genetic diagnostics of type 1 spinal muscular atrophy |
DK3714070T3 (en) * | 2017-11-20 | 2022-02-07 | Turun Yliopisto | Hitherto UNKNOWN CIP2A VARIANT AND USES THEREOF |
CA3116174A1 (en) * | 2018-10-31 | 2020-05-07 | Rutgers, The State University Of New Jersey | Gramc: genome-scale reporter assay method for cis-regulatory modules |
FR3088194B1 (en) * | 2018-11-09 | 2021-02-19 | Univ Paris Sud | USING THE MICRORNA MIR-27A-5P TO TREAT CLOSTRIDIUM DIFFICILE INTESTINAL INFLAMMATION |
KR102192455B1 (en) * | 2019-04-05 | 2020-12-17 | 한국과학기술원 | A cancer diagnosis marker based on ATAC-Seq and Method using the same |
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CN115803438A (en) * | 2020-05-19 | 2023-03-14 | 艾夏卡法国有限公司 | Promoter sequences for expressing gene therapy products in CD3+ cells in vitro and in vivo |
WO2021239307A1 (en) | 2020-05-27 | 2021-12-02 | Universität Zürich | Novel transduction enhancers and uses thereof |
IL301675A (en) * | 2020-10-09 | 2023-05-01 | Tenaya Therapeutics Inc | Plakophillin-2 gene therapy methods and compositions |
US11781156B2 (en) | 2020-10-09 | 2023-10-10 | Tenaya Therapeutics, Inc. | Plakophillin-2 gene therapy methods and compositions |
WO2022212766A2 (en) * | 2021-03-31 | 2022-10-06 | Hunterian Medicine Llc | Compact promoters for gene expression |
CN113265428B (en) * | 2021-06-11 | 2023-03-14 | 东北林业大学 | Detection system for constructing copper change in living cells by utilizing metallothionein and application |
WO2023150553A1 (en) * | 2022-02-01 | 2023-08-10 | University Of Rochester | Gpr17 promoter-based targeting and transduction of glial progenitor cells |
WO2024001172A1 (en) * | 2022-06-27 | 2024-01-04 | Ractigen Therapeutics | Oligonucleotide modulators activating complement factor h expression |
Family Cites Families (4)
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US6242211B1 (en) * | 1996-04-24 | 2001-06-05 | Terragen Discovery, Inc. | Methods for generating and screening novel metabolic pathways |
AU6015098A (en) * | 1997-02-24 | 1998-09-09 | Cornell Research Foundation Inc. | Method of screening agents as candidates for drugs or sources of drugs |
US6504084B1 (en) * | 1999-04-23 | 2003-01-07 | Pioneer Hi-Bred International, Inc. | Maize NPR1 polynucleotides and methods of use |
US20030211481A1 (en) * | 2002-05-08 | 2003-11-13 | Erives Albert J. | Method for identifying cellular targets |
-
2006
- 2006-12-08 EP EP06849046A patent/EP2021499A4/en not_active Ceased
- 2006-12-08 JP JP2008545677A patent/JP2009519710A/en not_active Withdrawn
- 2006-12-08 WO PCT/US2006/046920 patent/WO2007078599A2/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2007078599A9 (en) | 2007-10-04 |
EP2021499A4 (en) | 2010-02-17 |
JP2009519710A (en) | 2009-05-21 |
WO2007078599A2 (en) | 2007-07-12 |
EP2021499A2 (en) | 2009-02-11 |
WO2007078599A3 (en) | 2008-08-28 |
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