WO2007078599A3 - Functional arrays for high throughput characterization of gene expression regulatory elements - Google Patents

Functional arrays for high throughput characterization of gene expression regulatory elements Download PDF

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Publication number
WO2007078599A3
WO2007078599A3 PCT/US2006/046920 US2006046920W WO2007078599A3 WO 2007078599 A3 WO2007078599 A3 WO 2007078599A3 US 2006046920 W US2006046920 W US 2006046920W WO 2007078599 A3 WO2007078599 A3 WO 2007078599A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
regulatory elements
gene expression
high throughput
expression regulatory
Prior art date
Application number
PCT/US2006/046920
Other languages
French (fr)
Other versions
WO2007078599A9 (en
WO2007078599A2 (en
WO2007078599A8 (en
Inventor
Nathan D Trinklein
Shelley F Aldred
Sara J Cooper
Richard M Myers
Original Assignee
Univ Leland Stanford Junior
Nathan D Trinklein
Shelley F Aldred
Sara J Cooper
Richard M Myers
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/636,385 external-priority patent/US20070161031A1/en
Application filed by Univ Leland Stanford Junior, Nathan D Trinklein, Shelley F Aldred, Sara J Cooper, Richard M Myers filed Critical Univ Leland Stanford Junior
Priority to JP2008545677A priority Critical patent/JP2009519710A/en
Priority to EP06849046A priority patent/EP2021499A4/en
Publication of WO2007078599A2 publication Critical patent/WO2007078599A2/en
Publication of WO2007078599A9 publication Critical patent/WO2007078599A9/en
Publication of WO2007078599A3 publication Critical patent/WO2007078599A3/en
Publication of WO2007078599A8 publication Critical patent/WO2007078599A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Abstract

The present invention provides compositions, kits, assemblies, libraries, arrays, and high throughput methods for large scale structural and functional characterization of gene expression regulatory elements in a genome of an organism, especially in a human genome. In one aspect of the invention, an array of expression constructs is provided, e.ach of the expression constructs comprising: a nucleic acid segment operably linked with a reporter sequence in an expression vector such that expression of the reporter sequence is under the transcriptional control of the nucleic acid segment, the nucleic acid segment varying in the library and having a diversity of at least 50. The nucleic acid segments can be a large library of gene expression regulatory elements such as transcriptional promoters. The present invention can have a wide variety of applications such as in personalized medicine, pharmacogenomics, and correlation of polymorphisms with phenotypic traits.
PCT/US2006/046920 2005-12-16 2006-12-08 Functional arrays for high throughput characterization of gene expression regulatory elements WO2007078599A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2008545677A JP2009519710A (en) 2005-12-16 2006-12-08 Functional arrays for high-throughput characterization of gene expression regulatory elements
EP06849046A EP2021499A4 (en) 2005-12-16 2006-12-08 Functional arrays for high throughput characterization of gene expression regulatory elements

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US75092905P 2005-12-16 2005-12-16
US60/750,929 2005-12-16
US76205606P 2006-01-24 2006-01-24
US60/762,056 2006-01-24
US11/636,385 US20070161031A1 (en) 2005-12-16 2006-12-07 Functional arrays for high throughput characterization of gene expression regulatory elements
US11/636,385 2006-12-07

Publications (4)

Publication Number Publication Date
WO2007078599A2 WO2007078599A2 (en) 2007-07-12
WO2007078599A9 WO2007078599A9 (en) 2007-10-04
WO2007078599A3 true WO2007078599A3 (en) 2008-08-28
WO2007078599A8 WO2007078599A8 (en) 2008-10-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/046920 WO2007078599A2 (en) 2005-12-16 2006-12-08 Functional arrays for high throughput characterization of gene expression regulatory elements

Country Status (3)

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EP (1) EP2021499A4 (en)
JP (1) JP2009519710A (en)
WO (1) WO2007078599A2 (en)

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Also Published As

Publication number Publication date
WO2007078599A9 (en) 2007-10-04
EP2021499A4 (en) 2010-02-17
JP2009519710A (en) 2009-05-21
WO2007078599A2 (en) 2007-07-12
WO2007078599A8 (en) 2008-10-30
EP2021499A2 (en) 2009-02-11

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