WO2007052282A1

WO2007052282A1 - A plant derived biostimulant formulation and method for preparation thereof

Info

Publication number: WO2007052282A1
Application number: PCT/IN2006/000080
Authority: WO
Inventors: Mihir Govindji Virji
Original assignee: Nico Orgo Manures
Priority date: 2005-11-07
Filing date: 2006-03-09
Publication date: 2007-05-10

Abstract

The present invention discloses a plant-derived bio-stimulant and method of preparation thereof. A plant-derived bio-stimulant is prepared from Nicotina tabacum L. (Tobacco). It is prepared by controlled fermentation of suckers of tobacco by sour whey. It can be used as seedling dipping formulation and/or foliar spray. This formulation is completely natural product, non-toxic, neutral pH product, fully water soluble, compatible with most fungicides and insecticides.

Description

A PLANT DERIVED BIOSTIMULANT FORMULATION AND

METHOD FOR PREPARATION THEREOF

The present invention relates to a plant derived bio-stimulant and method of

preparation thereof.

The main object of the present invention is to provide bio-stimulant, which is

prepared from Nicotina tabacum L. (Tobacco). It is manufactured by controlled

fermentation of young and succulent leaf.

The state of prior art

Plants have regulated growth, pre-determined steps of differentiation, different

metabolic rates in cells, and at least partially a communication between cells too. Plant

hormones or phytohormones play important role in such type of activities. Plant

hormones or phytohormones are organic chemical compound produced in one part of

the body that has a target elsewhere in the body. Plants have five classes of hormones,

which include auxin, gibberellin, cytokinin, ethylene and abscisic acid. The cellular

exchange of material is ensured by perforations of the cell walls at regular intervals.

Auxin promotes cell division and growth, stem elongation. Auxin also plays

important role in inhibiting growth of lateral buds and thus maintains apical

dominance. It is also important in a plant movement called phototropism.

Gibberellins promote growth rates in the plant and increase plant size. It is

recognized that gibberellins are not produced in the stem tip. They stimulate the germination of pollen and the growth of pollen tubes. They induce the development of

parthenocarpous fruits like apples, pumpkin, and egg-plants twice the size of normal

fruit.

Cytokinins promote cell division. They reduce senescence and stimulate the

dark-germination of light-dependent seeds. They also help in the development of buds

as well as the germination of several seeds. Cytokinins are usually produced in roots,

young fruits, and in the seeds.

Ethylene promotes maturation and abscission of fruits. Ethylene regulates

senescence and fading of flowers and abscission of petals and leaves. Though ethylene

promotes maturation of crops, it is used to ripen fruits / crops.

The most striking effects of abscisic acid (ABA) are inhibition of growth and

maintenance of the dormancy of buds. It reverses the effects of growth stimulating

hormones in the several tissues. It also helps in the opening and closing of the

stomata.(From: http://www.biologie.uni-hamburg.de/b-online)

Patent No. RU2142231 describes use of alkaline salts of gibberellins with

product of processing fungus metabolizing with appearance of gibberellins, minerals

and trace minerals essential for vital activity of plants. This preparation increases

vegetative mass of rapidly growing green crops such as lettuce, parsley, dill, onion,

garlic, and celery.

Patent No. WO9400986 discloses plant growth regulating composition

comprising dry bend of an inorganic salt ingredient selected from alkali metal and ammonium bicarbonates and an effective plant growth regulating quantity of a

phytohormone ingredient.

Patent No. US4680889 describes a process for treating plants with sound of

particular frequency to stimulate or reduce the growth of the plant along with the plant

growth stimulating or inhibiting aqueous solution.

Patent No. CNl 162583 discloses a nutritive regulation agent for different

growing phases of vegetables is a concentrated liquor prepared from the acetic acid,

urea, potassium dihydrogen sulfate, calcium phosphate, sodium molybdate, copper

sulfate, humic acid, triacontanol, cytomin, boric acid and manganese sulfate.

In above-mentioned patent either natural/synthetic plant growth hormones or

humic acid substances are used as growth stimulating formulation. Also these are

complex formulations. It is noticed that in these formulations tobacco plant is not used.

However the use of tobacco in the form of fertilizer has been described in certain

patented invention.

Korean Patent Registration No. 1000201890000 discloses fertilizer prepared

from waste tobacco. In this invention a nitrogen and phosphate solution is mixed to

neutralize the alkali component of incompletely burned tobacco powder and leaves

where weight is decreased by 40-65% having 1.3-3% total nitrogen and 6.0-8.0%

water soluble potassium.

Patent No. CN1394485 relates to a nuisance-free plant pesticide and its

insecticidal organic fertilizer. It is made up from plant raw materials of wild aconite tuber, datura, nux vomica, oleander, pyrethrum, tobacco, camphor and white arsenic

through a certain preparation process. It is mixed with loess and dung so as to obtain

insecticidal organic fertilizer.

In these inventions tobacco is used in fertilizer preparation but for these

formulations other ingredients are also required. So there is a requirement of the

simple formulation, which enhance growth and production of field crops, vegetables,

flowers and fruit corps.

In the present invention a simple plant growth stimulating formulation is

prepared with the use of axillary growth of tobacco called suckers. The inclusion of

bacteria for fermentation has added advantages with respect to the efficacy of the

invention.

Detail Description of Invention

The main object of the present invention is to prepare plant derived bio-

stimulant formulation and method for preparation thereof.

In the present invention tobacco is used which is normally grown as an annual

and is potentially a woody, shrub like perennial. The axillary growths of tobaccos are

carefully selected which contain growth stimulating and protecting bio-substances.

After 60-70 days of planting of Nicotina tabacum L. the axillary growth (suckers)

having size of 10-15 cm in length are selected for the production of bio-stimulant. In

the present invention 20 Kg of axillary growth (suckers) of tobacco is used. These suckers are fermented with the use of sour whey (Butter Milk). Sour

whey contains Lactobacillus bacteria, which are gram-positive facultative bacteria and

convert lactose and other simple sugars to lactic acid. Lactobacilli are usually straight,

although they can form spiral or coccobacillary forms under certain conditions. They

are often found in pairs or chains of varying length. (From: http:/ biology.

Kenyon.edu/MicrobialJBiorealm/bacteria/gram- positive/lactobacillus.htm) 500 ml of

sour buttermilk is used for the fermentation process. Because of this specific

conentatrion the product quality is greatly improved.

Embodiment of the present invention relates process for preparing plant-derived

bio-stimulant, which involves following steps:

(a) Tobacco plant axillary growths (suckers) of definite size 10-15 cm

length are collected from field after 60-70 days of planting of

Nicotiana tabacum L.

(b) These suckers are allowed to putrify in a heap for 3-4 days.

(c) Suckers are loaded into a round fermentation vessel of 10,000 liter

capacity having height 3.47 m and diameter 1.91 m.

(d) For every 20 kg suckers, 500 ml of sour whey (sour butter milk) is

added to hasten the fermentation.

(e) When vessel is completely filled with material, 125-150 kg / m²

pressure is applied to compress the material and this is repeated several times by putting additional quantities of suckers and

proportionate amount of whey,

(f) The leachate starts eluting from the vessel after 7 days of

fermentation.

(g) The leachates are collected in a barrel of 200 liters capacity,

(h) The leachates are filtered through very fine cloth and stored,

(i) The leachates are allowed to stabilize for a period of six months and

packed into plastic bottle for use.

The product has following physico-chemical characteristics.

Physical:

1. Color : Dark Brown

2. Specific Gravity : 1.00- 1.02

3. Solid Content : 29-31 mg/ml

Chemical:

1. Total Nitrogen : 0.20 - 0.24 % w/v

2. Total Phosphorus ( P2O5) : 0.076 - 0.20 % w/v

3. Total Potash ( K2O) : 1.39- 1.64 % w/v

4. Total Calicum (Ca) : 0.09-0.18 % w/v

5. Organic Carbon : 0.65-1.83 % w/v

6. Humic Acid : 1.12-3.15 % w/v

7. pH : 7.69-7.98 8. Total Amino Acid : 0.24-0.25 % w/v

9. Fe : 25.9-38.5 ppm

10. Mn : 0.42-1.01 ppm

11. Zn : 1.76-1.88 ppm

12.Cu : 0.22-0.27 ppm

The shelf life of a finished product is more than two years.

This formulation represents a formidable advance in harnessing bio-chemical

growth stimulants for the benefit of agriculture, floriculture and horticulture. A plant

derived bio-stimulant formulation is useful for improvement in fruit set and retention,

increasing content of soluble solids in tomatoes and water melons, improvement in

quality of onions improvement in quality, size and bearing of hybrid roses.

It can be used as seedling dipping formulation and/or foliar spray for higher

productivity of farm crops, fruits and vegetables and flowers.

Dose:

For Seedling dipping:

• 25 ml of plant derived bio-stimulant formulation is mixed with 1 liter of water.

• The rootlet is dipped for 10 minutes, removed and made ready for

transplantation.

For Spray

• 10ml of plant derived bio-stimulant formulation is mixed with one liter of

water. • About 200L of this volume is sprayed to one acre. (About 2-3 times during the

crop-growing season).

• 1^st spray: One month after transplantation

• 2^nd spray: Two month after transplantation

• 3^rd spray: At the on set of flowering.

A plant derived bio-stimulant formulation completely natural product, non¬

toxic, neutral pH product, fully water soluble, compatible with most fungicides and

pesticides.

Claims

We claim,

1. A plant derived bio-stimulant formulation and method of preparation thereof

comprises 20 Kg of axillary growths (suckers) of tobacco and 500 ml of sour

whey (butter milk).

2. The method of preparation of plant derived bio-stimulant formulation

comprises steps of:

(a) selecting tobacco plant axillary growths (suckers) from field after

60-70 days of planting of ^'Nicotiana tabacum L.;

(b) allowing these suckers to putrify in a heap for 3-4 days;

(c) loading suckers into a round fermentation vessel of 10,000 liter

capacity having height 3.47 m and diameter 1.91 m;

(d) adding sour whey (sour butter milk) to hasten the fermentation;

(e) applying 125-150 kg / m² pressure when vessel is completely

filled with material to compress the material and repeating several

times by putting additional quantities of suckers and proportionate

amount of whey;

(f) eluting the leachate from the vessel after 7 days of fermentation;

(g) collecting the leachates in a barrel of 200 liters capacity;

(h) filtering the leachates through very fine cloth and stored;

(i) stabilizing the leachates for a period of six months and packing

into plastic bottle for use.

3. The method of preparation of plant derived bio-stimulant formulation as

claimed in claim 2 step (a) wherein, suckers having length of 10-15 cm are

selected.

4. A plant derived bio-stimulant formulation and method of preparation thereof as

substantially herein describes with foregoing description.