WO2007018744A2 - Biosynthese sans cellule d'acide nucleique - Google Patents
Biosynthese sans cellule d'acide nucleique Download PDFInfo
- Publication number
- WO2007018744A2 WO2007018744A2 PCT/US2006/023439 US2006023439W WO2007018744A2 WO 2007018744 A2 WO2007018744 A2 WO 2007018744A2 US 2006023439 W US2006023439 W US 2006023439W WO 2007018744 A2 WO2007018744 A2 WO 2007018744A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- therapeutic
- dna
- composition according
- research
- sequence
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
Abstract
La présente invention concerne un processus permettant de produire un ADN de haute qualité dans un système sans cellule, exempt d'impuretés bactériennes et exempt de manière optimale de séquences codantes pour des gènes bactériens qui peuvent minimiser ou rendre silencieux une expression de gène lorsqu'elle est utilisée pour l'expression à l'intérieur d'une cellule cible. Ce système sans cellule est un procédé rapide qui fournit un produit final hautement fidèle, plus propre convenant pour des applications thérapeutiques avec moins d'efforts et de dépenses et, qui peut être adapté à l'amplification de matrices de type plasmide manquant de séquences de gènes plasmide bactériennes non nécessaires. Ceci permet d'accroître l'efficacité du système et d'accroître l'efficacité du vecteur d'expression de produit final. Ce produit final peut être facilement utilisé comme ADN thérapeutique du fait de la faible incidence des composants de cellule bactérienne et des toxines bactériennes. Cette invention concerne aussi un procédé de production d'ADN pour quelque recherche ou quelque but thérapeutique que ce soit, qui est sensiblement exempt d'impuretés de cellule bactérienne, ainsi qu'une thérapeutique de produit fini comprenant des vaccins ADN.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US70534805P | 2005-08-04 | 2005-08-04 | |
US60/705,348 | 2005-08-04 | ||
USPCTUS22000/045028 | 2005-12-12 | ||
PCT/US2005/045028 WO2006063355A2 (fr) | 2004-12-11 | 2005-12-12 | Biosynthese acellulaire d'acide nucleique de haute qualite et utilisations correspondantes |
US76217206P | 2006-01-25 | 2006-01-25 | |
US60/762,172 | 2006-01-25 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007018744A2 true WO2007018744A2 (fr) | 2007-02-15 |
WO2007018744A3 WO2007018744A3 (fr) | 2008-07-03 |
Family
ID=37728257
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2006/023439 WO2007018744A2 (fr) | 2005-08-03 | 2006-06-14 | Biosynthese sans cellule d'acide nucleique |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2007018744A2 (fr) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010005365A1 (fr) * | 2008-07-09 | 2010-01-14 | General Electric Company | Produit d'amplification par cercle roulant non traité |
US8921072B2 (en) | 2008-09-02 | 2014-12-30 | General Electric Compnay | Methods to generate DNA mini-circles |
US9125845B2 (en) | 2008-07-09 | 2015-09-08 | General Electric Company | DNA vaccines, uses for unprocessed rolling circle amplification product and methods for making the same |
US11324839B2 (en) | 2019-09-18 | 2022-05-10 | Intergalactic Therapeutics, Inc. b | Synthetic DNA vectors and methods of use |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030190608A1 (en) * | 1999-11-12 | 2003-10-09 | Gary Blackburn | Microfluidic devices comprising biochannels |
WO2005003389A2 (fr) * | 2003-06-28 | 2005-01-13 | Royal Holloway And Bedford New College | Amplification in vitro de l'adn |
WO2006063355A2 (fr) * | 2004-12-11 | 2006-06-15 | Cytogenix , Inc. | Biosynthese acellulaire d'acide nucleique de haute qualite et utilisations correspondantes |
-
2006
- 2006-06-14 WO PCT/US2006/023439 patent/WO2007018744A2/fr active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20030190608A1 (en) * | 1999-11-12 | 2003-10-09 | Gary Blackburn | Microfluidic devices comprising biochannels |
WO2005003389A2 (fr) * | 2003-06-28 | 2005-01-13 | Royal Holloway And Bedford New College | Amplification in vitro de l'adn |
WO2006063355A2 (fr) * | 2004-12-11 | 2006-06-15 | Cytogenix , Inc. | Biosynthese acellulaire d'acide nucleique de haute qualite et utilisations correspondantes |
Non-Patent Citations (6)
Title |
---|
CAMPÀS ET AL: "DNA biochip arraying, detection and amplification strategies" TRENDS IN ANALYTICAL CHEMISTRY, vol. 23, January 2004 (2004-01), pages 49-62, XP004484688 * |
DEAN ET AL: "Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multiply-primed rolling circle amplification" GENOME RESEARCH, vol. 11, 2001, pages 1095-1099, XP002261245 cited in the application * |
DEMIDOV: "10 years of rolling the minicircles: RCA assays in DNA diagnostics" EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, vol. 5, July 2005 (2005-07), pages 477-478, XP009074526 * |
LUTHRA ET AL: "Isothermal multiple displacement amplification. A highly reliable approach for generating unlimited high molecular weight genomic DNA from clinical specimens" JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 6, August 2004 (2004-08), pages 236-242, XP002371240 * |
RECTOR ET AL: "A sequence-independent strategy for detection and cloning of circular DNA virus genomes by using multiply primed rolling-circle amplification" JOURNAL OF VIROLOGY, vol. 78, May 2004 (2004-05), pages 4993-4998, XP002392144 * |
WANG ET AL: "DNA amplification method tolerant to sample degradation" GENOME RESEARCH, vol. 14, November 2004 (2004-11), pages 2357-2366, XP002391762 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010005365A1 (fr) * | 2008-07-09 | 2010-01-14 | General Electric Company | Produit d'amplification par cercle roulant non traité |
JP2011527570A (ja) * | 2008-07-09 | 2011-11-04 | ゼネラル・エレクトリック・カンパニイ | 未処理rca産物 |
US9125845B2 (en) | 2008-07-09 | 2015-09-08 | General Electric Company | DNA vaccines, uses for unprocessed rolling circle amplification product and methods for making the same |
US8921072B2 (en) | 2008-09-02 | 2014-12-30 | General Electric Compnay | Methods to generate DNA mini-circles |
US11324839B2 (en) | 2019-09-18 | 2022-05-10 | Intergalactic Therapeutics, Inc. b | Synthetic DNA vectors and methods of use |
US11602569B2 (en) | 2019-09-18 | 2023-03-14 | Intergalactic Therapeutics, Inc. | Synthetic DNA vectors and methods of use |
US11684680B2 (en) | 2019-09-18 | 2023-06-27 | Intergalactic Therapeutics, Inc. | Synthetic DNA vectors and methods of use |
US11766490B2 (en) | 2019-09-18 | 2023-09-26 | Intergalactic Therapeutics, Inc. | Synthetic DNA vectors and methods of use |
Also Published As
Publication number | Publication date |
---|---|
WO2007018744A3 (fr) | 2008-07-03 |
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