WO2006084642A3 - Microorganisms with a disruption of the respiratory chain gene rcs26 for the production of vitamin c - Google Patents

Microorganisms with a disruption of the respiratory chain gene rcs26 for the production of vitamin c Download PDF

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Publication number
WO2006084642A3
WO2006084642A3 PCT/EP2006/001008 EP2006001008W WO2006084642A3 WO 2006084642 A3 WO2006084642 A3 WO 2006084642A3 EP 2006001008 W EP2006001008 W EP 2006001008W WO 2006084642 A3 WO2006084642 A3 WO 2006084642A3
Authority
WO
WIPO (PCT)
Prior art keywords
production
vitamin
microorganisms
polynucleotides
relates
Prior art date
Application number
PCT/EP2006/001008
Other languages
French (fr)
Other versions
WO2006084642A2 (en
Inventor
Masako Shinjoh
Original Assignee
Dsm Ip Assets Bv
Masako Shinjoh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv, Masako Shinjoh filed Critical Dsm Ip Assets Bv
Publication of WO2006084642A2 publication Critical patent/WO2006084642A2/en
Publication of WO2006084642A3 publication Critical patent/WO2006084642A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids
    • C12P7/60—2-Ketogulonic acid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
PCT/EP2006/001008 2005-02-11 2006-02-06 Microorganisms with a disruption of the respiratory chain gene rcs26 for the production of vitamin c WO2006084642A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05405138 2005-02-11
EP05405138.8 2005-02-11

Publications (2)

Publication Number Publication Date
WO2006084642A2 WO2006084642A2 (en) 2006-08-17
WO2006084642A3 true WO2006084642A3 (en) 2007-04-19

Family

ID=36202156

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/001008 WO2006084642A2 (en) 2005-02-11 2006-02-06 Microorganisms with a disruption of the respiratory chain gene rcs26 for the production of vitamin c

Country Status (1)

Country Link
WO (1) WO2006084642A2 (en)

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 24 January 2005 (2005-01-24), "Gluconobacter oxydans 621H, complete genome.", XP002379245, retrieved from EBI accession no. EM_PRO:CP000009 Database accession no. CP000009 *
DEPPENMEIER U ET AL: "Biochemistry and biotechnological applications of Gluconobacter strains", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER VERLAG, BERLIN, DE, vol. 60, no. 3, November 2002 (2002-11-01), pages 233 - 242, XP002334995, ISSN: 0175-7598 *
MACAULEY S ET AL: "THE GENUS GLUCONOBACTER AND ITS APPLICATIONS IN BIOTECHNOLOGY", CRC CRITICAL REVIEWS IN BIOTECHNOLOGY, CRC PRESS, BOCA RATON, FL, US, vol. 21, no. 1, 2001, pages 1 - 25, XP008026977, ISSN: 0738-8551 *
PRUST C ET AL: "Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 23, no. 2, 23 January 2005 (2005-01-23), pages 195 - 200, XP002334906, ISSN: 1087-0156 *
SUGISAWA T ET AL: "ISOLATION AND CHARACTERIZATION OF A NEW VITAMIN C PRODUCING ENZYME (L-GULONO-GAMMA-LACTONE DEHYDROGENASE) OF BACTERIAL ORIGIN", BIOSCIENCE, BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 59, no. 2, February 1995 (1995-02-01), pages 190 - 196, XP001084987, ISSN: 0916-8451 *

Also Published As

Publication number Publication date
WO2006084642A2 (en) 2006-08-17

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