WO2006084722A3 - Gene sts 15 - Google Patents

Gene sts 15 Download PDF

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Publication number
WO2006084722A3
WO2006084722A3 PCT/EP2006/001217 EP2006001217W WO2006084722A3 WO 2006084722 A3 WO2006084722 A3 WO 2006084722A3 EP 2006001217 W EP2006001217 W EP 2006001217W WO 2006084722 A3 WO2006084722 A3 WO 2006084722A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotides
production
vitamin
relates
polypeptides
Prior art date
Application number
PCT/EP2006/001217
Other languages
French (fr)
Other versions
WO2006084722A2 (en
Inventor
Bastien Chevreux
Anne Francoise Mayer
Anja Meury
Nigel John Mouncey
Masako Shinjoh
Original Assignee
Dsm Ip Assets Bv
Bastien Chevreux
Anne Francoise Mayer
Anja Meury
Nigel John Mouncey
Masako Shinjoh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv, Bastien Chevreux, Anne Francoise Mayer, Anja Meury, Nigel John Mouncey, Masako Shinjoh filed Critical Dsm Ip Assets Bv
Publication of WO2006084722A2 publication Critical patent/WO2006084722A2/en
Publication of WO2006084722A3 publication Critical patent/WO2006084722A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids
    • C12P7/60—2-Ketogulonic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
PCT/EP2006/001217 2005-02-11 2006-02-10 Gene sts 15 WO2006084722A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP05405081 2005-02-11
EP05405066 2005-02-11
EP05405066.1 2005-02-11
EP05405081.0 2005-02-11

Publications (2)

Publication Number Publication Date
WO2006084722A2 WO2006084722A2 (en) 2006-08-17
WO2006084722A3 true WO2006084722A3 (en) 2006-09-28

Family

ID=36636268

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/001217 WO2006084722A2 (en) 2005-02-11 2006-02-10 Gene sts 15

Country Status (1)

Country Link
WO (1) WO2006084722A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004029268A1 (en) * 2002-09-27 2004-04-08 Dsm Ip Assets B.V. Microbial production of vitamin c

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004029268A1 (en) * 2002-09-27 2004-04-08 Dsm Ip Assets B.V. Microbial production of vitamin c

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHENG ET AL: "Molecular cloning and functional expression of d-arabitol dehydrogenase gene from Gluconobacter oxydans in Escherichia coli", FEMS MICROBIOLOGY LETTERS, AMSTERDAM, NL, vol. 252, no. 1, 1 November 2005 (2005-11-01), pages 35 - 42, XP005121712, ISSN: 0378-1097 *
DATABASE EMBL [online] 19 March 2005 (2005-03-19), "Gluconobacter oxydans polyol ABC transporter permease (dalT) and D-arabitol dehydrogenase (dalD) genes, complete cds.", XP002390747, retrieved from EBI accession no. EM_PRO:AY940143 Database accession no. AY940143 *
DATABASE EMBL [online] 24 January 2005 (2005-01-24), "Gluconobacter oxydans 621H, complete genome.", XP002390746, retrieved from EBI accession no. EM_PRO:CP000009 Database accession no. CP000009 *
PRUST CHRISTINA ET AL: "Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 23, no. 2, 23 January 2005 (2005-01-23), pages 195 - 200, XP002334994, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2006084722A2 (en) 2006-08-17

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