WO2006084644A3 - Gene rcs 33 - Google Patents

Gene rcs 33 Download PDF

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Publication number
WO2006084644A3
WO2006084644A3 PCT/EP2006/001010 EP2006001010W WO2006084644A3 WO 2006084644 A3 WO2006084644 A3 WO 2006084644A3 EP 2006001010 W EP2006001010 W EP 2006001010W WO 2006084644 A3 WO2006084644 A3 WO 2006084644A3
Authority
WO
WIPO (PCT)
Prior art keywords
polynucleotides
production
vitamin
relates
microorganisms
Prior art date
Application number
PCT/EP2006/001010
Other languages
French (fr)
Other versions
WO2006084644A2 (en
Inventor
Masako Shinjoh
Original Assignee
Dsm Ip Assets Bv
Masako Shinjoh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv, Masako Shinjoh filed Critical Dsm Ip Assets Bv
Publication of WO2006084644A2 publication Critical patent/WO2006084644A2/en
Publication of WO2006084644A3 publication Critical patent/WO2006084644A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
PCT/EP2006/001010 2005-02-11 2006-02-06 Gene rcs 33 WO2006084644A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05405178.4 2005-02-11
EP05405178 2005-02-11

Publications (2)

Publication Number Publication Date
WO2006084644A2 WO2006084644A2 (en) 2006-08-17
WO2006084644A3 true WO2006084644A3 (en) 2006-12-14

Family

ID=36552967

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/001010 WO2006084644A2 (en) 2005-02-11 2006-02-06 Gene rcs 33

Country Status (1)

Country Link
WO (1) WO2006084644A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003104445A1 (en) * 2002-06-06 2003-12-18 Roche Vitamins Ag Aldehyde dehydrogenase
WO2004029268A1 (en) * 2002-09-27 2004-04-08 Dsm Ip Assets B.V. Microbial production of vitamin c
WO2004029269A1 (en) * 2002-09-27 2004-04-08 Dsm Ip Assets B.V. Process for producing vitamin c

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003104445A1 (en) * 2002-06-06 2003-12-18 Roche Vitamins Ag Aldehyde dehydrogenase
WO2004029268A1 (en) * 2002-09-27 2004-04-08 Dsm Ip Assets B.V. Microbial production of vitamin c
WO2004029269A1 (en) * 2002-09-27 2004-04-08 Dsm Ip Assets B.V. Process for producing vitamin c

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 24 January 2005 (2005-01-24), "Gluconobacter oxydans 621H, complete genome.", XP002384679, retrieved from EBI accession no. EM_PRO:CP000009 Database accession no. CP000009 *
DATABASE EMBL [online] 30 July 2004 (2004-07-30), "Gluconacetobacter diazotrophicus heme exporter protein C (ccmC), unknown protein, cytochrome C biosynthesis heme-carrier protein (ccmE), and cytochrome C-type biogenesis protein (ccmF) genes, complete cds; and thioredoxin disulfide isomerase (ccmG) gene, partial cds.", XP002384680, retrieved from EBI accession no. EM_PRO:AY456185 Database accession no. AY456185 *
LEE S ET AL: "Indole-3-acetic acid biosynthesis is deficient in Gluconacetobacter diazotrophicus strains with mutations in cytochrome c biogenesis genes", JOURNAL OF BACTERIOLOGY 2004 UNITED STATES, vol. 186, no. 16, 2004, pages 5384 - 5391, XP002384676, ISSN: 0021-9193 *
PRUST C ET AL: "Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 23, no. 2, 23 January 2005 (2005-01-23), pages 195 - 200, XP002377530, ISSN: 1087-0156 *
TOYAMA H ET AL: "Quinohemoprotein alcohol dehydrogenases: structure, function, and physiology", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, NEW YORK, US, US, vol. 428, no. 1, 1 August 2004 (2004-08-01), pages 10 - 21, XP004517983, ISSN: 0003-9861 *

Also Published As

Publication number Publication date
WO2006084644A2 (en) 2006-08-17

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