WO2006084490A1 - Makroporöses kunststoffperlenmaterial - Google Patents
Makroporöses kunststoffperlenmaterial Download PDFInfo
- Publication number
- WO2006084490A1 WO2006084490A1 PCT/EP2005/011907 EP2005011907W WO2006084490A1 WO 2006084490 A1 WO2006084490 A1 WO 2006084490A1 EP 2005011907 W EP2005011907 W EP 2005011907W WO 2006084490 A1 WO2006084490 A1 WO 2006084490A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- monomers
- plastic bead
- bead material
- material according
- polymerizable monomers
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F2/00—Processes of polymerisation
- C08F2/12—Polymerisation in non-solvents
- C08F2/16—Aqueous medium
- C08F2/18—Suspension polymerisation
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
- C08F220/04—Acids; Metal salts or ammonium salts thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
- C08F220/10—Esters
- C08F220/26—Esters containing oxygen in addition to the carboxy oxygen
- C08F220/32—Esters containing oxygen in addition to the carboxy oxygen containing epoxy radicals
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
- C08F220/52—Amides or imides
- C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
- C08F222/36—Amides or imides
- C08F222/38—Amides
- C08F222/385—Monomers containing two or more (meth)acrylamide groups, e.g. N,N'-methylenebisacrylamide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
Definitions
- the invention relates to a macroporous plastic bead material.
- the plastic bead material is a crosslinked copolymer of hydrophilic, vinylic polymerizable monomers and bond active against ligands with nucleophilic groups.
- the invention further relates to a process for the preparation of the macroporous plastic bead material by inverse suspension polymerization of a monomer phase, as well as its uses.
- Porous polymeric support materials for proteins, in particular enzymes are well known.
- Application areas are in the medical field, eg. B. in the enzymatic cleavage of ß-lactam antibiotics such as penicillin G to 6-aminopenicillan acid (6-APA) by means of penicillin acylase (penicillin amidase).
- ß-lactam antibiotics such as penicillin G to 6-aminopenicillan acid (6-APA) by means of penicillin acylase (penicillin amidase).
- penicillin acylase penicillin amidase
- DE-OS 22 37 316 describes a process for the preparation of peripiform crosslinked copolymers by free-radical polymerization of a radical-forming initiator-containing monomer mixture containing a biologically active substances binding monomer, a crosslinking comonomer and at least one other comonomer, wherein the monomer mixture in a nonpolar organic Liquid is suspended to droplets and polymerized.
- Suitable as non-polar organic liquid in particular aliphatic hydrocarbons, especially those having 6 or more carbon atoms.
- the examples use mixtures of n-heptane and perchlorethylene.
- the ratio of the monomer phase to the continuous organic phase may be between 1: 1 and 1:10, but ratios between 1: 1, 5 and 1: 4 are preferred.
- DE-A 31 06 456 describes a comparison with DE-OS 22 37 316 improved in relation to the binding capacity of the polymer beads method.
- Particularly high binding capacities for proteins, in particular for the enzyme penicillin acylase (penicillin amidase) are obtained when the carrier polymers have high contents of crosslinking monomers and when the monomer phase, formed from the monomers and the diluent, contains a solvent mixture as diluent. Suitable mixtures may, for. B be water / methanol or formamide / methanol. Monomers and diluents are approximately in the ratio of 1: 2.6.
- a mixture of n-hexane and perchlorethylene is used for the organic, continuous phase.
- the ratio of the monomer phase to the continuous organic phase is approximately 1: 2.8 in the examples.
- carrier polymers with a binding capacity, measured as penicillin acylase activity, of up to 125 U / g can be obtained.
- the carrier polymer material is characterized inter alia by a binding capacity for penicillin amidase of at least 220 [U / g wet] and at the same time a low swelling number of at most 1.5.
- EP 1 352 957 A1 describes binding active carrier materials containing epoxide groups for the immobilization of enzymes.
- the described support material has the advantage that enzymes can be covalently bonded even at low ionic strengths.
- the functionality is achieved in a process in which, in the case of carrier bead materials having epoxide groups on the surface, part of the epoxide groups are subsequently reacted with various reagents. This results in additional amino groups, which favor the binding of the enzymes at low ionic strengths in the surrounding medium.
- the disadvantages include u.a.
- the biomacromolecules to be immobilized may be partially affected or denatured by a high salt content.
- EP 1 352 957 A1 proposes a solution to this problem by reacting some of the epoxide groups subsequently with different reagents, starting from carrier bead materials having epoxide groups on the surface. This results in additional amino groups, which favor the binding of the enzymes at low ionic strengths in the surrounding medium. This process has the disadvantage that it is expensive and thereby makes the production of the carrier polymer materials more expensive.
- a macroporous plastic bead material should be provided, which enables a covalent binding of biomacromolecules at comparatively low ionic strength.
- a complex subsequent modification as described in EP 1 352 957 A1, should be avoided.
- the swelling number should have an acceptable value of not higher than 2.5.
- macroporous plastic bead material having an average particle diameter of 10 to 1000 ⁇ m radically polymerized from the following monomer types
- hydrophilic monomers are meant those monomers which form at room temperature at least 10% aqueous solutions and preferably contain no ionic or ionizable by acid or base addition groups.
- the monomers a), b), c) and d) add up to 100 wt .-%.
- the monomers a) are 5 to 40, preferably 5 to 20, in particular 6 to 10,% by weight of hydrophilic free-radically polymerizable monomers having a vinyl group which form at room temperature at least 10% aqueous solutions.
- Monomers a) are not vinylically polymerizable monomers having a quaternary amino group. The monomers a) are therefore always different from the monomers d).
- monomers a in particular acrylamide and / or methacrylamide are suitable, with methacrylamide being preferred.
- hydroxyalkyl esters of unsaturated polymerizable carboxylic acids such as hydroxyethyl acrylate and hydroxyethyl methacrylate or N-vinylpyrrolidone.
- Monomers b) are 5 to 50, preferably 32 to 40,% by weight of free-radically polymerizable monomers having a vinyl group and an additional functional group, preferably an oxirane group (epoxy group), which can form covalent bonds in a polymer-analogous reaction with the nucleophilic groups of the ligands ,
- oxirane groups are suitable for binding ligands while maintaining their biological activity.
- Preferred monomers b) are glycidyl methacrylate and / or allyl glycidyl ether. Both monomers are particularly preferably simultaneously used in approximately equal amounts, so that they together give a proportion of 30-50, preferably 32-40 wt .-%.
- Monomers c) are 20-60, in particular 25-55, particularly preferably 40-55 wt .-% hydrophilic, crosslinking radically polymerizable monomers having two or more ethylenically unsaturated polymerizable groups.
- Preferred monomers c) are N, N 'methylene-bis-acrylamide or N, N' - methylene-bis-methacrylamide. NN 'methylene-bis-methacrylamide is particularly preferred.
- Suitable are hydrophilic di (meth) acrylates, such as.
- Monomers d) are 1 to 20, preferably 5 to 15, in particular 8 to 12 wt .-% vinylic polymerizable monomers having a quaternary amino group, preferably alkyl (meth) acrylate monomers having a quaternary amino group in the alkyl radical.
- Monomers d) are preferably trimethylammoniumethyl methacrylate or trimethylammoniumethyl methacrylate chloride.
- the macroporous plastic bead material is preferably a copolymer of the following monomers:
- a particularly preferred composition is where the proportions of the five stated monomers of the monomer types a), b), c) and d) add up to 100% by weight:
- the invention thus relates to a
- a), b) c) and d) add up to 100 wt .-% and the ratio of the monomers to the diluent 1: 1, 5 to 1: 2.5, preferably 1: 1, 7 to 1 Is 2.3, and the diluent used is a mixture of methanol and water in the ratio 1: 1, 0 to 1: 4.0, the monomer phase being in a continuous phase of an organic solvent of an aliphatic hydrocarbon having 5 to 7 Carbon atoms is distributed to droplets, wherein the ratio of monomer phase to continuous phase
- the monomer phase consists of the monomers a), b), c) and d), which are dissolved in a diluent, which must be a mixture of methanol and water in the ratio 1: 1, 0 to 1: 4.0.
- a diluent which must be a mixture of methanol and water in the ratio 1: 1, 0 to 1: 4.0.
- Particularly favorable mixing ratios for methanol and water are 1: 1, 2 to 1: 2.5, in particular 1: 1, 3 to 1: 1, 7.
- ratio of monomers to diluents This must be in the range from 1: 1, 5 to 1: 2.5, preferably 1: 1, 7 to 1: 2.3, particularly preferably in the range of 1, 9 to 2.1.
- an organic solvent which is an aliphatic hydrocarbon having 4 to 7 carbon atoms is suitable. Preference is given to n-heptane and particularly preferably cyclohexane.
- the ratio of the monomer phase to the continuous phase formed by the organic solvent must be 1: 1.5 to 1: 4.0, preferably 1: 2.0 to 1: 3.0.
- Other Anlagensbedinqunqen
- the suspended monomer phase contains in a conventional manner polymerization initiators, preference is given to sulfur-free initiators, particular preference is given to 4,4 * azobis (4-valeric acid), as well as protective colloids (emulsifiers), such as.
- B a copolymer of 95 parts of n-butyl methacrylate and 5 parts of 2-trimethylammoniumethlyl methacrylate chloride having molecular weights (weight average) in the range of 30,000 to 80,000.
- the perl polymerization (also referred to as suspension polymerization) is otherwise carried out in a known manner by z. B.
- the continuous phase is presented with the protective colloid and the monomer phase, in which the initiator is, with stirring z. B. at 40 to 60 0 C in the organic phase and then heated to 60 - 70 0 C.
- the water / methanol mixture may, for. B. are removed almost completely azeotropically over a period of 6 hours.
- the batch is allowed to finish for about 3 to 5 hours and then cooled to room temperature.
- the resulting beads are sucked off and z. B. dried for 12 hours in vacuo.
- the bead polymers can also be filtered off and washed with water and then used moist or dried.
- the drying is preferably carried out in a fluidized-bed dryer, since in this way solvent residues can be removed particularly effectively.
- An important application of the carrier polymer material according to the invention is the cleavage of penicillin G to 6-aminopenicillan-acid (6-APA) by means of bound penicillin amidase from E. coli.
- the binding capacity is understood as meaning that enzymatic activity which can be achieved with maximum loading of the carrier polymer material with a specific enzyme.
- the binding capacity is expressed as penicillin amidase activity in units per g of carrier polymer beads [U / g wet].
- the binding capacity of the carrier polymer beads according to the invention is at least 200 [U / g wet] in this measurement method.
- the macroporous bead plastic material of the invention has a binding capacity for penicillin amidase from E. coli of at least 200 [U / g wet], resulting from the reaction of 1530 units (units) of penicillin amidase with 1 g of carrier polymer material, in the presence of a salt concentration of at most 0.1 at most 0.05 [mol / l], on.
- the salt concentration is calculated from the salt optionally present in the enzyme solution and the immobilized salt or buffer salt in the immobilization mixture.
- carrier polymer material 1 g is added to 1530 units of penicillin amidase in 5 ml of sterile potassium phosphate buffer pH 7.5 and incubated for 48 hours at 23 0 C.
- the polymer beads are then placed on a sintered glass frit (porosity 2 or 3) and washed twice with deionized water and then twice with 0.1 M potassium phosphate buffer pH 7.5 containing 0.05% ethyl 4-hydroxybenzoate Absauggen washed on the frit. The wet weight of the resulting penicillin-acylase loaded beads is determined.
- the polymer beads are obtained as in a) via a glass frit by means of suction through 20 ml of deionized water.
- Binding capacity is reported as penicillin amidase units per g of wet carrier polymer material (U / g wet). One unit corresponds to one ⁇ mole of hydrolyzed penicillin G per minute ( ⁇ mol / min);
- 1 l of 0.5M NaOH is equivalent to 500 ⁇ mol of hydrolyzed penicillin G. (The water content of the carrier polymer material is approximately constant and can therefore be neglected.)
- PcA units used PcA per g of polymer carrier dry matter Pavliere
- the swellability of the polymer beads in water is expressed by the swelling number [ml wet / ml dry].
- the macroporous plastic bead material according to the invention has a swelling index in water of greater than 1.5 to 2.5, preferably from 1.7 to 2.3.
- the swelling number is thus higher than in the case of the plastic bead material according to DE 198 04 518 C2 ( ⁇ 1, 5) and lower than in the case of the plastic bead material according to DE 34 04 021 A1 (about 3.0).
- the carrier polymer materials of this invention can be used to covalently bond ligands using the existing oxirane groups in stirred or flow-through reactors. This can be z. B. by addition of proteins, in particular enzymes, from concentrated solutions covalent bonding while maintaining their biological activity. Furthermore, peptides, amino acids, ß-lactam antibiotics, lipids, nucleotides, polynucleotides, low molecular weight nucleophilic compounds or metalloraganische compounds with the oxirane groups of the carrier beads can be implemented.
- the polymer beads loaded with ligands can be prepared in a manner known per se for the stereospecific synthesis of chiral substances, such as amino acids (D-phenylalanine, p-hydroxy-D-phenylalanine, L-tert-leucine) or medicaments, for.
- ibuprofen used. They are also used as carriers in the enzymatic cleavage of penicillin G to 6-aminopenicillinic acid (6-APA), cephalosporin G to 7-aminodesacetoxycephalosporanic acid (7-ADCA) or cephalosporin C to 7-aminocephalosporanic acid (7-ACA). The method is described in DECHEMA Annual Meeting 1996 - Abstracts, Vol. 1, DECHEMA eV.
- polymer beads can also be used in the separation technique for adsorption chromatography or gel permeation chromatography. For specific adsorption, the polymer beads may be loaded with immunoglobulin fractions from antisera or with monoclonal antibody.
- a further field of use is the use of the carrier polymer material loaded with enzymes or antibodies as an adsorbent in extracorporeal therapy, in which pathogenic or toxic substances are removed from whole blood.
- the macroporous plastic bead material according to the invention can be used in particular:
- the macroporous plastic bead material of the present invention because of the vinylic polymerizable monomers having a quaternary amino group, enables the physical adsorption of ligands, e.g. B enzymes, via ionic interactions independent of the pH of the immobilization mixture.
- ligands e.g. B enzymes
- the preparation of the plastic bead material is possible in one step without post-treatments of the polymer.
- ligands can be covalently bound in good yield even at extremely low salt content in a manner similar to that of the material EP 1 352 957 A1.
- the swelling number of the plastic bead material is in acceptable ranges. Examples
- carrier polymer material 1 g is added to 1530 units of penicillin amidase in 5 ml of sterile potassium phosphate buffer pH 7.5 and incubated for 48 hours at 23 0 C.
- the polymer beads are then placed on a sintered glass frit (porosity 2 or 3) and washed twice with deionized water and then twice with 0.1 M potassium phosphate buffer pH 7.5 containing 0.05% ethyl 4-hydroxybenzoate Absauggen washed on the frit. The wet weight of the resulting penicillin-acylase loaded beads is determined. b) Determination of the binding capacity
- the polymer beads are obtained as in a) via a glass frit by means of suction through 20 ml of deionized water.
- Binding capacity is reported as penicillin amidase units per g of wet carrier polymer material (U / g wet). One unit corresponds to one ⁇ mole of hydrolyzed penicillin G per minute ( ⁇ mol / min);
- PcA units used PcA per g of polymer carrier dry matter
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007554436A JP2008530280A (ja) | 2005-02-08 | 2005-11-08 | マクロ孔質プラスチックパール材料 |
EP05801988A EP1851257A1 (de) | 2005-02-08 | 2005-11-08 | Makroporöses kunststoffperlenmaterial |
US11/719,969 US20070259968A1 (en) | 2005-02-08 | 2005-11-08 | Macroporous Plastic Bead |
IL185019A IL185019A0 (en) | 2005-02-08 | 2007-08-02 | Macroporous plastic bead material |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102005005828A DE102005005828A1 (de) | 2005-02-08 | 2005-02-08 | Makroporöses Kunststoffperlenmaterial |
DE102005005828.0 | 2005-02-08 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2006084490A1 true WO2006084490A1 (de) | 2006-08-17 |
Family
ID=35717434
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2005/011907 WO2006084490A1 (de) | 2005-02-08 | 2005-11-08 | Makroporöses kunststoffperlenmaterial |
Country Status (9)
Country | Link |
---|---|
US (1) | US20070259968A1 (de) |
EP (1) | EP1851257A1 (de) |
JP (1) | JP2008530280A (de) |
KR (1) | KR20070114120A (de) |
CN (1) | CN101084248A (de) |
DE (1) | DE102005005828A1 (de) |
IL (1) | IL185019A0 (de) |
TW (1) | TW200635611A (de) |
WO (1) | WO2006084490A1 (de) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9958421B2 (en) * | 2009-12-22 | 2018-05-01 | Ge Healthcare Bioprocess R&D Ab | Method for dry packing chromatography columns |
KR102316276B1 (ko) | 2020-04-20 | 2021-10-25 | 한국과학기술연구원 | 다단기공구조를 갖는 킬레이트 복합체 및 그 제조방법 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4070348A (en) * | 1973-07-25 | 1978-01-24 | Rohm Gmbh | Water-swellable, bead copolymer |
WO1999040122A1 (de) * | 1998-02-05 | 1999-08-12 | Röhm Gmbh | Vorrichtung zur herstellung von trägerpolymermaterialien in form von porösen polymerperlen |
WO2004039854A2 (de) * | 2002-10-31 | 2004-05-13 | Röhm Gmbh & Co.Kg | Makroporöses kunststoffperlenmaterial |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3106456A1 (de) * | 1981-02-21 | 1982-10-07 | Röhm GmbH, 6100 Darmstadt | Verfahren zur herstellung von perlfoermigen, hydrophilen, gegenueber proteinen bindungsaktiven traegerpolymeren |
DE3505920A1 (de) * | 1985-02-21 | 1986-08-21 | Röhm GmbH, 6100 Darmstadt | Schwach vernetzte, in wasser schnell quellende, teilchenfoermige, feste polymerisate oder mischpolymerisate, verfahren zu ihrer herstellung und ihre verwendung in hygieneartikeln |
DE3537259A1 (de) * | 1985-10-19 | 1987-04-23 | Hoechst Ag | Copolymerisat, verfahren zu seiner herstellung und seine verwendung als enzymtraeger |
DE60040850D1 (de) * | 1999-07-16 | 2009-01-02 | Calgon Corp | Wasserlösliche polymerzubereitung und verfahren zu deren anwendung |
JP3434800B2 (ja) * | 2001-01-31 | 2003-08-11 | 海洋科学技術センター | 地殻コア試料の採取方法、並びにこれに用いる抗菌性高分子ゲルおよびゲル材料 |
DE10256656A1 (de) * | 2002-12-03 | 2004-06-17 | Röhm GmbH & Co. KG | Verfahren zur Herstellung von Cephalexin |
-
2005
- 2005-02-08 DE DE102005005828A patent/DE102005005828A1/de not_active Withdrawn
- 2005-11-08 EP EP05801988A patent/EP1851257A1/de not_active Withdrawn
- 2005-11-08 CN CNA2005800439696A patent/CN101084248A/zh active Pending
- 2005-11-08 JP JP2007554436A patent/JP2008530280A/ja active Pending
- 2005-11-08 US US11/719,969 patent/US20070259968A1/en not_active Abandoned
- 2005-11-08 WO PCT/EP2005/011907 patent/WO2006084490A1/de active Application Filing
- 2005-11-08 KR KR1020077018152A patent/KR20070114120A/ko not_active Application Discontinuation
- 2005-11-16 TW TW094140338A patent/TW200635611A/zh unknown
-
2007
- 2007-08-02 IL IL185019A patent/IL185019A0/en unknown
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4070348A (en) * | 1973-07-25 | 1978-01-24 | Rohm Gmbh | Water-swellable, bead copolymer |
WO1999040122A1 (de) * | 1998-02-05 | 1999-08-12 | Röhm Gmbh | Vorrichtung zur herstellung von trägerpolymermaterialien in form von porösen polymerperlen |
WO2004039854A2 (de) * | 2002-10-31 | 2004-05-13 | Röhm Gmbh & Co.Kg | Makroporöses kunststoffperlenmaterial |
Also Published As
Publication number | Publication date |
---|---|
TW200635611A (en) | 2006-10-16 |
EP1851257A1 (de) | 2007-11-07 |
KR20070114120A (ko) | 2007-11-29 |
JP2008530280A (ja) | 2008-08-07 |
DE102005005828A1 (de) | 2006-08-17 |
CN101084248A (zh) | 2007-12-05 |
IL185019A0 (en) | 2007-12-03 |
US20070259968A1 (en) | 2007-11-08 |
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