WO2006040871A1 - Plaque a puits et dispositif pour cultures cellulaires - Google Patents

Plaque a puits et dispositif pour cultures cellulaires Download PDF

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Publication number
WO2006040871A1
WO2006040871A1 PCT/JP2005/014082 JP2005014082W WO2006040871A1 WO 2006040871 A1 WO2006040871 A1 WO 2006040871A1 JP 2005014082 W JP2005014082 W JP 2005014082W WO 2006040871 A1 WO2006040871 A1 WO 2006040871A1
Authority
WO
WIPO (PCT)
Prior art keywords
cell culture
well
well plate
cells
cell
Prior art date
Application number
PCT/JP2005/014082
Other languages
English (en)
Japanese (ja)
Inventor
Yohei Yamada
Mikako Saito
Hideaki Matsuoka
Original Assignee
Chuo Precision Industrial Co., Ltd.
Tokyo University Of Agriculture And Technology National University Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chuo Precision Industrial Co., Ltd., Tokyo University Of Agriculture And Technology National University Corporation filed Critical Chuo Precision Industrial Co., Ltd.
Publication of WO2006040871A1 publication Critical patent/WO2006040871A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples

Definitions

  • the present invention relates to a well plate and a cell culture instrument suitable for use in a single cell manipulation support robot that manipulates cells using a manipulator.
  • a cell plate and a cell culture instrument for culturing are disclosed by independently accommodating cells in each of a plurality of wells! /, For example, disclosed in Patent Document 1 Such cell-containing multi-microwells and culture dishes are already known.
  • Patent Document 2 As a general well structure, for example, Patent Document 2 and that disclosed in Patent Document 3 have been proposed.
  • Each of these conventional well plates is formed by forming a plurality of wells that contain cells into a cylindrical shape.
  • the movement direction of the carrier when performing operations such as cell holding or injection is inclined with respect to the surface of the table surface of the well plate.
  • multiple wells that contain cells are formed in a cylindrical shape, so the edges of the openings of each well interfere with the beam and cause troubles such as cell retention and injection. was there.
  • Patent Document 1 International Publication No. WO 2004/015055 A1 Pamphlet (FIG. 3, FIG. 4)
  • Patent Document 2 JP 2001-252068 A (Fig. 2)
  • Patent Document 3 Republished Patent WOOOZ 17316 ( Figure 2)
  • an object of the present invention is to eliminate the disadvantages of the above-described conventional technology, and even when the moving direction of the manipulator or capillary is inclined with respect to the surface of the well plate, the cells can be safely processed.
  • the object is to provide a well plate and a cell culture instrument capable of performing operations such as holding and injection.
  • the wall plate of the present invention is a wall plate having a plurality of tools for accommodating cells, and in order to achieve the above-mentioned problem, in particular, the inner diameter of the well is directed from the bottom side of the well toward the opening. Accordingly, it has a configuration characterized in that the diameter is gradually increased.
  • the inner diameter in the vicinity of the bottom of the well is sufficiently small compared to the opening, so that the cells may inadvertently move on the bottom of the well to capture and inject the injection. If the work becomes difficult, there will be no negative effects.
  • the inner diameter of the well can be gradually increased from the bottom surface toward the opening.
  • the inner wall of the well may be formed using a downwardly convex quadric surface or a downwardly convex spherical surface! /.
  • the inner diameter of the well can be gradually increased from the bottom surface toward the opening.
  • the apex angle of the conical slope is in the range of 40 degrees to 140 degrees.
  • This value is equivalent to a range of 20 to 70 degrees when it is replaced by the slope of the slope relative to the surface of the table plate, and the actual slope is within this range.
  • Specified according to the inclination of the beam the apex angle of the conical slope is less than several degrees is interpreted as the draft angle in the normal molding technology using the core pin, and the technical idea different from this application Therefore, the claim power is also excluded.
  • a recess for holding cells may be formed on the bottom surface of the tool.
  • the cell culture instrument of the present invention has a configuration characterized in that the above-described well plate is integrally formed on the bottom surface of the cell culture container in order to achieve the same problem as described above.
  • the shape of the well provided on the well plate is uniform, it is possible to recombine the nested insert with the core pin forming the well or the dummy insert with the surface forming the inner side of the bottom of the cell culture vessel.
  • a modular mold it is possible to provide a cell culture device having a well plate having a desired group of wells.
  • the above-mentioned well plate may be fixed on the bottom surface of the cell culture container to constitute a cell culture instrument.
  • a well plate formed separately from the cell culture container is adhered to the bottom surface of the cell culture container by adhesion or ultrasonic welding to constitute a cell culture instrument. Since dishes, glass slides, etc. can be used as cell culture containers, the user can use various types of cell culture containers that have been used in the past or that are suitable for the purpose of experimentation. A dish, slide glass, etc. can be freely selected and used.
  • the manufacturing of the wel plate is the same as described above, and a modular that allows recombination of nesting-like nestings with a nesting with a core pin forming a wel and a pressing surface that contacts the parting line of the facing mold.
  • a mold By using a mold, it is possible to provide a cell culture instrument having a well plate having a desired group of wells. The invention's effect
  • the wel plate of the present invention is formed so that the inner diameter of the wel gradually increases from the bottom surface side of the wel to the opening side, and the inner diameter of the wel is increased in the vicinity of the wel opening.
  • the inner diameter of the well is made small enough in the vicinity of the bottom of the well, so that the opening of the capillary and the well that moves toward the cell by moving the directional force inclined with respect to the surface of the table plate. The cell can be held and injected without any interference, and the cells can be moved inadvertently on the bottom of the well, making it difficult to capture and inject by the gyro. Evil is also eliminated
  • the concave portion for holding the cells is formed on the bottom surface of the tool, it is possible to reliably prevent inadvertent movement of the cells on the bottom surface of the well, and to capture and inject the cells with the use of a pill. Can be facilitated.
  • the cell culture instrument of the present invention is a cell culture instrument in which the well plate is integrally formed on the bottom surface of the cell culture container.
  • the cell culture instrument has the same effects as described above and is suitable for mass production of cell culture instruments.
  • a cell culture instrument when configured by fixing a well plate on the bottom surface of a cell culture container, an existing dish or a slide glass can be used as the cell culture container.
  • the user can use the familiar cell culture container or actual Various cell culture containers suitable for the purpose of experimentation, in other words, dishes and slide glasses can be freely selected and used.
  • FIG. 1 is a front view showing the overall configuration of a single-cell manipulation support robot suitable for so-called injection work such as injection of a gene or a drug into a single cell.
  • the single-cell manipulation support robot 1 is roughly composed of a robot body 2 and a controller 3.
  • the robot body 2 is composed of a table 4 for placing a cell culture vessel such as a dish and a cell plate, and a simulator 5 for manipulating the cells in the cell culture device. , A column 8 for supporting the stage 7, and a microscope 9 fixed for observing cells in the cell culture instrument.
  • stage 7 The structure of stage 7 is shown in FIG.
  • the table 4 provided on the stage 7 is driven in a horizontal two-axis horizontal plane by the driving means of each axis consisting of a stepping motor equal force, and the manipulator 5 arranged on the right side of the table 4 has a stepping motor equal force. It is driven independently in the space of three orthogonal axes by the driving means of each axis and the driving means composed of piezo actuators and the like.
  • the structure of the manipulator 6 deployed on the left side of the table 4 is substantially the same.
  • the manipulator 6 arranged on the left side of the table 4 has a cavity 10 for holding a single cell arranged on the well plate 14 of the cell culture device 13 by means such as suction.
  • the mapper 5 provided on the right side of the table 4 includes a capillary 11 having an injection hole at the tip for injecting a gene or a drug with respect to a single cell as an end effector.
  • the manipulators 5 and 6 are inclined symmetrically with respect to the normal of the table 4 and installed at an angle of 45 degrees, for example. This is to avoid interference between Lee 10 and 11, and also to ensure the field of view of the microscope 9.
  • the manipulators 5 and 6 need to be large enough to contain the driving means. Since the pillars 10 and 11 need to be thick enough to secure a certain level of rigidity, they can be installed in parallel with the couplers 5 and 6 and the capillary pillars 10 and 11 in parallel. It is practically impossible to perform simultaneous operations on the, and thus there is a situation in which it must be installed in a state tilted with respect to the normal of the table 4.
  • a device holder 12 for performing rough positioning when placing the cell culture device 13 is fixed to the table 4, and the cell culture device is placed on the table 4 via the device holder 12. 13 comes to be placed.
  • the main part of the controller 3 for driving and controlling each part of the single-cell operation support robot 1 is constituted by a controller body 3a. 'The first operation panel 3R, second operation panel 3L, keyboard 3b with mouse, foot switch 3c, and monitor 3d are connected.
  • the first operation panel 3R is a manual operation means for selectively driving and controlling the table 4 and the manipulator 5 arranged on the right side thereof. Whether it is set to 5 can be selected by operating the head switch 16R provided at the tip of the joystick 15R.
  • the movement of the table 4 and the manipulator 5 in the horizontal plane is controlled by the joystick 15R or the track ball 17R, and the movement of the cab 11 as the end effector of the manipulator 5 is controlled by the rotation operation of the tip of the joystick 15R.
  • the second operation panel 3L is a manual operation means for driving and controlling the manipulator 6 arranged on the left side of the table 4.
  • the movement of the manipulator 6 in the horizontal plane is controlled by the joystick 15L or the trackball 17L, and the movement of the manipulator 6 in the axial direction, which is the end effector 10 of the manipulator 6, is controlled by rotating the tip of the joystick 15L.
  • FIG. 3 is a plan view showing a configuration example of the cell culture instrument 13.
  • the cell culture instrument 13 includes a cell culture container 18 made of a conventional dish and a well plate 14 fixed to the bottom surface 19 of the cell culture container 18.
  • both the cell culture container 18 and the well plate 14 are formed of a synthetic resin such as acrylic. In such a case, it is also possible to use ultrasonic welding or the like.
  • a part of the weld plate 14 is enlarged and shown in the plan view of FIG.
  • the wall plate 14 is formed in a rectangular thin plate as a whole, and a large number of wells 20 for containing cells are provided in a matrix so as to penetrate the front and back sides.
  • protrusions 21 are provided in a lattice pattern on the front surface of the well plate 14 so as to partition each of the wells 20, and mineral oil or the like is stored in a rectangular section partitioned by the protrusions 21.
  • FIG. 4 (b) is a cross-sectional view showing the well plate 14 broken along the line AA in FIG. 4 (a).
  • each of the wels 20 is formed such that its inner diameter gradually increases from the bottom surface side of the wel 20 toward the opening side.
  • the bottom of the well 20 is blocked by the bottom surface 19 of the cell culture vessel 18.
  • Fig. 4 (b) shows an example in which the inner wall 22 of the wel 20 is formed by a conical slope, but the inner wall of the wel 20 is utilized by using a downwardly convex quadratic curved surface or a downwardly convex spherical curved surface. It is also possible to form 22.
  • the apex angle ⁇ of the conical slope is restricted to a range of 40 degrees to 140 degrees. This numerical value is equivalent to a range of 20 to 70 degrees when it is replaced by the slope of the slope ⁇ relative to the surface of the table surface 14 and the actual slope slope j8 is within this range.
  • the manipulators 5 and 6 and the capillaries 10 and 11 are inclined at an angle of 45 degrees (45 degrees relative to the normal), so the slope slope j8 is 45 degrees or less, that is, the top of the conical slope.
  • the angle ⁇ By preventing the angle ⁇ from being over 90 degrees, interference between the tip of the capillary 10 and 11 moving in the direction inclined 45 ° with respect to the surface of the table plate 14 and the opening of the well 20 is prevented. Can do.
  • the minimum value of the apex angle ⁇ of the conical slope should theoretically be 40 degrees or less. May be possible.
  • the capillaries 10 and 11 need to have a certain thickness, and the tips of the capillaries 10 and 11 are tapered to realize simultaneous operation of the capillaries 10 and 11 on a small single cell. Therefore, no matter what structure is applied, as long as these capillaries 10 and 11 are used, there is no chance that the minimum value of the apex angle ⁇ will be less than a few degrees, for example.
  • the structure of the ul 20 where the apex angle ⁇ of the conical slope is less than several degrees is not in accordance with the gist of the present invention.
  • the maximum value of the apex angle ⁇ of the conical slope is set to 140 degrees because, if the angle formed by the capillaries 10 and 11 is 140 degrees or more, the edge of the cell culture vessel 18 that also has a conventional dish force will be This is because the tips of 10 and 11 may interfere.
  • the well plate 14 having the structure shown in FIGS. 4 (a) and 4 (b) is formed by applying a known injection molding technique or the like, and the lower surface of the well plate 14 is bonded or superposed. By adhering to the bottom surface 19 of the cell culture vessel 18 by means such as sonic welding, a cell culture instrument 13 as shown in FIG. 3 is obtained. In this case, the bottom surface of the well 20 is the same as the bottom surface 19 of the cell culture vessel 18.
  • a first nesting composed of a rectangular core block main body provided with an IJ insert equivalent to a width of 1Z2 of 21 and a core pin for forming a well integrally formed on the front end surface of the core block main body. And a dummy that has a length equal to the total length of the first nesting including the core block body and the core pin, and has a flat pressing surface that abuts against the parting line of the mold facing the tip. It is desirable to use a modular mold equipped with a powerful second nesting and a cotter block or the like that holds the first and second nestings in a freely recombinable manner.
  • the well plate 14 may be integrally formed on the bottom surface 19 of the cell culture container 18 by using injection molding or the like.
  • a rectangular core block body provided with a cut corresponding to the width of 1Z2 of the protrusion 21 on the outer periphery of the tip, and a core pin and force for forming a well integrally formed on the tip surface of the core block body.
  • the first nesting and a length equivalent to the total length of the first nesting including the core block body and the core pin, and the tip surface forms a part of the inner surface of the bottom surface 19 of the cell culture container 18.
  • a wel with a desired group of wels By using a modular mold with a second nesting made of dummies and a cotter block or the like for holding the first and second nestings in a recombinable manner, a wel with a desired group of wels.
  • a cell culture vessel 18 having a plate 14 can be produced.
  • the bottom 23 of the well 20 is connected to the lower end of the inner wall 22 that also has a conical slope equal force, and the diameter of the recess 23 for holding cells is smaller than the opening of the well 20.
  • the depth of the recess 23 should be equal to or less than the thickness of the cell to be operated so that the capillaries 10 and 11 do not interfere with the inner peripheral wall of the recess 23 and obstruct the cell capture or injection operation.
  • FIG. 4 (c) describes the cylindrical recess 23
  • the recess 23 may have other shapes.
  • the wel plate 14 ' can be formed separately or integrally with the cell culture vessel 18 in the same manner as the wel plate 14 described above.
  • a slide glass 24 or the like as shown in FIG. 5 is used as a cell culture container, and the well plates 14 and 14 'are fixed to the slide glass 24, or are molded integrally to form a cell. It can also be a culture device 25.
  • various known techniques such as laser sputtering, printing, and engraving (engraving) can be used as the means for forming the weld 20.
  • FIG. 1 is a front view showing the overall configuration of a single-cell manipulation support robot.
  • FIG. 2 is an enlarged front view showing a stage of a single cell manipulation support robot.
  • FIG. 3 is a plan view showing a configuration example of a cell culture instrument composed of a dish and a well plate.
  • FIG. 4 A diagram showing a configuration example of a well plate.
  • Fig. 4 (a) is an enlarged plan view showing a part of the well plate
  • Fig. 4 (b) is a sectional view thereof
  • Fig. 4 (c) is a cross-sectional view showing the ulplate with different modes.
  • FIG. 5 is a plan view showing a configuration example of a cell culture instrument composed of a slide glass and a well plate.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des plaques à puits permettant des opérations telles que la saisie et l'injection de cellules sans créer de problème même si les sens de déplacement d'un manipulateur et des capillaires sont inclinés par rapport aux surfaces des plaques à puits, ainsi qu'un dispositif pour les cultures cellulaires. On empêche l’interférence des capillaires (10, 11) qui se déplacent dans des directions inclinées par rapport aux surfaces des plaques à puits (14, 14') avec les parties ouvertes des puits (20) en augmentant le diamètre intérieur des puits (20) près des parties ouvertes de façon à ce que les opérations telles que la saisie et l'injection de cellules puissent être réalisées sans créer de problème. En outre, selon l'invention, on peut empêcher le déplacement des cellules par inadvertance sur les surfaces inférieures des puits (20) en diminuant le diamètre intérieur des puits (20) près des parties de fond des puits de sorte que les opérations telles que la saisie et l'injection de cellules sont facilement réalisées.
PCT/JP2005/014082 2004-10-12 2005-08-02 Plaque a puits et dispositif pour cultures cellulaires WO2006040871A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004-298134 2004-10-12
JP2004298134A JP2006109715A (ja) 2004-10-12 2004-10-12 ウェルプレートおよび細胞培養器具

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Publication Number Publication Date
WO2006040871A1 true WO2006040871A1 (fr) 2006-04-20

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015129577A1 (fr) * 2014-02-27 2015-09-03 学校法人近畿大学 Dispositif de culture cellulaire et procédé de culture cellulaire utilisant ledit dispositif de culture cellulaire
GB2539935A (en) * 2015-07-01 2017-01-04 Insphero Ag Device for propagating microtissues
WO2017108087A1 (fr) * 2015-12-21 2017-06-29 Curevac Ag Incrustation pour plaque de culture et procédé de préparation de système de plaque de culture correspondant doté d'une telle incrustation

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JP2010041960A (ja) 2008-08-13 2010-02-25 Fujitsu Ltd 捕捉シャーレ、マイクロインジェクション装置、及び、マイクロインジェクション方法
JP5776956B2 (ja) * 2009-02-09 2015-09-09 大日本印刷株式会社 細胞培養容器
JP6278590B2 (ja) * 2012-11-02 2018-02-14 大日本印刷株式会社 細胞培養容器および細胞培養方法
US20160312164A1 (en) * 2013-12-12 2016-10-27 Yamaha Hatsudoki Kabushiki Kaisha Well plate and subject selection device provided with well plate
WO2016046938A1 (fr) * 2014-09-25 2016-03-31 株式会社サイフューズ Support pour cellules et dispositif, procédé et système de production d'une structure cellulaire
JP6427424B2 (ja) * 2015-01-19 2018-11-21 ヤマハ発動機株式会社 対象物の操作方法
WO2017134787A1 (fr) * 2016-02-04 2017-08-10 株式会社サイフューズ Plateau de cellules, et dispositif de production d'une structure cellulaire, procédé, et système
JP6454830B1 (ja) * 2017-10-10 2019-01-16 株式会社サイフューズ 細胞トレイおよび不織布組立部材
US20190344265A1 (en) * 2018-05-08 2019-11-14 Lidong Qin Networked Cell Holder Chip

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Publication number Priority date Publication date Assignee Title
JP2000069957A (ja) * 1998-09-02 2000-03-07 Asahi Techno Glass Corp 蓋付き容器
WO2000017316A1 (fr) * 1998-09-22 2000-03-30 Sumitomo Bakelite Co., Ltd. Plaque a cupules multiples, pour la congelation de cellules de culture
WO2004015055A1 (fr) * 2002-08-08 2004-02-19 National University Corporation Tokyo University Of Agriculture And Technology Robot pouvant mettre en oeuvre des operations sur une cellule unique
JP2004254622A (ja) * 2003-02-26 2004-09-16 Yamanashi Tlo:Kk 胚性幹細胞（ｅｓ細胞）の胚様体（ｅｂ）形成のための培養容器及び培養方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000069957A (ja) * 1998-09-02 2000-03-07 Asahi Techno Glass Corp 蓋付き容器
WO2000017316A1 (fr) * 1998-09-22 2000-03-30 Sumitomo Bakelite Co., Ltd. Plaque a cupules multiples, pour la congelation de cellules de culture
WO2004015055A1 (fr) * 2002-08-08 2004-02-19 National University Corporation Tokyo University Of Agriculture And Technology Robot pouvant mettre en oeuvre des operations sur une cellule unique
JP2004254622A (ja) * 2003-02-26 2004-09-16 Yamanashi Tlo:Kk 胚性幹細胞（ｅｓ細胞）の胚様体（ｅｂ）形成のための培養容器及び培養方法

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015129577A1 (fr) * 2014-02-27 2015-09-03 学校法人近畿大学 Dispositif de culture cellulaire et procédé de culture cellulaire utilisant ledit dispositif de culture cellulaire
JPWO2015129577A1 (ja) * 2014-02-27 2017-03-30 学校法人近畿大学 細胞培養治具およびこの細胞培養治具を用いた細胞培養方法
GB2539935A (en) * 2015-07-01 2017-01-04 Insphero Ag Device for propagating microtissues
US10704016B2 (en) 2015-07-01 2020-07-07 Insphero Ag Device for propagating microtissues
WO2017108087A1 (fr) * 2015-12-21 2017-06-29 Curevac Ag Incrustation pour plaque de culture et procédé de préparation de système de plaque de culture correspondant doté d'une telle incrustation

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