WO2006037570A3 - Typ-sglt1-protein-assay - Google Patents

Typ-sglt1-protein-assay Download PDF

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Publication number
WO2006037570A3
WO2006037570A3 PCT/EP2005/010598 EP2005010598W WO2006037570A3 WO 2006037570 A3 WO2006037570 A3 WO 2006037570A3 EP 2005010598 W EP2005010598 W EP 2005010598W WO 2006037570 A3 WO2006037570 A3 WO 2006037570A3
Authority
WO
WIPO (PCT)
Prior art keywords
sglt1
active substance
type
sglt1 protein
protein assay
Prior art date
Application number
PCT/EP2005/010598
Other languages
English (en)
French (fr)
Other versions
WO2006037570A2 (de
Inventor
Natalie Watzke
Maarten Ruitenberg
Wolfgang Doerner
Renate Gauss
Bela Kelety
Kerstin Diekert
Original Assignee
Iongate Biosciences Gmbh
Natalie Watzke
Maarten Ruitenberg
Wolfgang Doerner
Renate Gauss
Bela Kelety
Kerstin Diekert
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iongate Biosciences Gmbh, Natalie Watzke, Maarten Ruitenberg, Wolfgang Doerner, Renate Gauss, Bela Kelety, Kerstin Diekert filed Critical Iongate Biosciences Gmbh
Publication of WO2006037570A2 publication Critical patent/WO2006037570A2/de
Publication of WO2006037570A3 publication Critical patent/WO2006037570A3/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Vorgeschlagen wird ein Verfahren zum Identifizieren eines Wirkstoffes, welcher eine enzymatische Eigenschaft des SGLT1-Proteins modifiziert. Es werden Primärträger bereitgestellt, welche das SGLT1-Protein im Bereich ihrer Membranen enthalten. Die Primärträger werden im Oberflächenbereich einer als Sekundärträger dienenden Biosensorelektrode angelagert. Es wird ein potenzieller Wirkstoff bereitgestellt. Der potenzielle Wirkstoff wird mit dem SGLT1-Protein zur Wechselwirkung gebracht. Bestimmt wird der qualitative und/oder quantitative Einfluss des potenziellen Wirkstoffes durch Detektieren einer elektrischen Aktion des SGLT1-Proteins oder deren Änderung über die Biosensorelektrode als Sekundärträger , z.B. mittels einer elektrischen Strommessung oder Spannungsmessung.
PCT/EP2005/010598 2004-10-01 2005-09-30 Typ-sglt1-protein-assay WO2006037570A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200410048397 DE102004048397B4 (de) 2004-10-01 2004-10-01 Typ-SGLT1-Protein-Assay
DE102004048397.3 2004-10-01

Publications (2)

Publication Number Publication Date
WO2006037570A2 WO2006037570A2 (de) 2006-04-13
WO2006037570A3 true WO2006037570A3 (de) 2006-06-08

Family

ID=35897398

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/010598 WO2006037570A2 (de) 2004-10-01 2005-09-30 Typ-sglt1-protein-assay

Country Status (2)

Country Link
DE (1) DE102004048397B4 (de)
WO (1) WO2006037570A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006011925B3 (de) * 2006-03-15 2007-08-23 Iongate Biosciences Gmbh Typ-PAT1-Protein-Assay
EP2226076A1 (de) 2009-02-25 2010-09-08 Henning Vollert Pflanzlicher Extrakt zur Prophylaxe und Behandlung von hyperglykämischen Erkrankungen

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1400529A1 (de) * 2001-05-30 2004-03-24 Kissei Pharmaceutical Co., Ltd. Glucopyranosyloxypyrazol derivat, diese enthaltende medizinische zusammensetzung, dessen medizinische verwendung und zwischenprodukt zu dessen herstellung
DE10244129A1 (de) * 2002-09-23 2004-04-01 Iongate Biosciences Gmbh H+-K+-ATPase-Assay

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10112505C1 (de) * 2001-03-15 2003-01-30 Iongate Biosciences Gmbh Sensoranordnung und Vorrichtung zur amperometrischen und/oder potentiometrischen, pharmakologischen Wirkort- und/oder Wirkstofftestung sowie Verfahren zur amperometrischen und/oder potentiometrischen, pharmakologischen Wirkort- und/oder Wirkstofftestung

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1400529A1 (de) * 2001-05-30 2004-03-24 Kissei Pharmaceutical Co., Ltd. Glucopyranosyloxypyrazol derivat, diese enthaltende medizinische zusammensetzung, dessen medizinische verwendung und zwischenprodukt zu dessen herstellung
DE10244129A1 (de) * 2002-09-23 2004-04-01 Iongate Biosciences Gmbh H+-K+-ATPase-Assay

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Patch clamp", XP002371666, Retrieved from the Internet <URL:http://en.wikipedia.org/wiki/Patch_clamp> [retrieved on 20060306] *
GUDRUN ANNA SAUER: "Untersuchungen zum Glucose-Auswärtstransport des Na+/Glucosetransporter SGLT1", ONLINE HOCHSCHULPUBLIKATIONSSYSTEM DER UNIVERSITÄTSBIBLIOTHEK FRANKFURT/MAIN, 2002, XP002371665, Retrieved from the Internet <URL:http://publikationen.ub.uni-frankfurt.de/volltexte/2003/180/pdf/00000282.pdf> [retrieved on 20060306] *
HABER C ET AL: "A NOVEL ELECTROGENIC SCREENING TOOL FOR MEMBRANE TRANSPORTER ASSAYS", AMERICAN BIOTECHNOLOGY LABORATORY, SHELTON, CT, US, vol. 23, no. 9, August 2005 (2005-08-01), pages 18 - 20, XP009059242, ISSN: 0749-3223 *

Also Published As

Publication number Publication date
DE102004048397A1 (de) 2006-04-13
DE102004048397B4 (de) 2007-08-16
WO2006037570A2 (de) 2006-04-13

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