WO2005117923A1

WO2005117923A1 - Bioprotective

Info

Publication number: WO2005117923A1
Application number: PCT/EP2005/005547
Authority: WO
Inventors: Björn-Oliver JACKISCH; Arno Cordes
Original assignee: Süd-Chemie AG
Priority date: 2004-05-27
Filing date: 2005-05-22
Publication date: 2005-12-15
Also published as: CN101001636A; MXPA06013619A; DE102004025869A1; BRPI0511563A; TW200608986A

Abstract

The invention relates to a method for health promotion, in particular, for control, treatment and/or prevention of diseases in aquatic organisms, using a composition comprising at least one bacterium of the type Brevibacillus parabrevis .

Description

BIOPROTEKTIVUM

The invention relates to a method for promoting health, in particular for combating and / or preventing diseases of aquatic organisms, using a composition which contains at least one brevibacillus parabrevis bacterium. Also described are additives containing at least one brevibacillus parabrevis bacterium and their preferred use.

The commercial production of aquatic life in spatially and temporally limited systems is called aquaculture. Today it represents an essential economic factor. The share of aquaculture in the world production of fish, shellfish and mussels was already 27.3% in 2000 (source: FAO). Annual growth rates in production of> 10% can be observed especially in Asian countries.

It is generally accepted that aquaculture helps reduce the pressure on wild species. Likewise, however, discusses that aquaculture, for example through the use of pharmaceuticals and the excessive introduction of nutrients into water, has negative effects on the environment.

To increase the space-time yield in culture systems, in addition to the energy content of feed, the culture density, i.e. maximizes the number of living things produced per unit area. Increasing culture densities go hand in hand both in water and on land with an increasing exposure to - often pathogenic - microorganisms. In addition, the use of fresh and / or live feed, e.g. Cuttlefish, polychaete, Artemia nauplia or microalgae, especially in the early culture phases of the production of aquatic organisms, additional pathogenic microorganisms that adhere to the feed are introduced into the culture environment. In addition, the constant presence of nutrient-rich feed in the culture tanks is ideal for the growth of bacteria.

Antibiotics are used therapeutically and prophylactically in considerable amounts to minimize the contamination of the cultural environment with microorganisms. The use of antibiotics is of great importance in the discussion about the negative effects of aquaculture. Two aspects in particular are discussed: on the one hand the introduction of antibiotics into the food chain and on the other hand the formation of antibiotic-resistant microorganisms with all their implications, especially with regard to increasing their pathogenicity potential.

The antibiotics used in aquaculture can be divided into four groups according to their mode of action. On the one hand, this is a group of substances that inhibit cell wall synthesis (penicillins and cephalosporins). This group includes, for example, penicillin, ampicillin, cefuroxi, Cefaclor, Vancomycin or Bacitracin. The second group includes substances that inhibit protein synthesis. It includes substances such as gentamycin, kanamycin, streptomycin, tetracycline, chloramphenicol or erythromycin. Inhibitors of nucleic acid synthesis such as sulphametizol, trimethoprim or nalidixic acid represent the third group. The fourth group includes substances which are thought to have an antimicrobial effect, but whose mechanism of action is not exactly understood. This includes, for example, inhibitory furanones (see, for example, WO 02/47681 for a comprehensive description of the use of these substances for controlling pathogenic organisms).

As already described above, these and other antibiotics are used to minimize pathogenic microorganisms in aquaculture and thus economic damage from diseases. Against the background of the described negative effects of direct antibiotics in aquaculture systems, alternative approaches to reducing pathogenic microorganisms have also been pursued in the past. For example, the indirect entry of antibiotics or vaccines by Artemia sp. (Dixon et al. (1995) Journal of Aquatic Animal Heal th 7, 42-45) or bacteria (Gomez-Gil et al. (1998) Applied and Environmental Microbiology 64, 2318-2322; Oggioni et al. (2003) Vaccine 21, 96-101). WO 03/103692 also describes the production and use of zooplankton, which is enriched with probiotics and prebiotics. The absorption and digestion of the carrier organisms mediate a direct release of the active substances in the target organism. It should be noted that this method is very complex and is also associated with a transition of the antibiotics into the food chain. The use of ozone to reduce the microbial load in aquacultures is also known from the scientific literature (Colberg & Lingg (1978) Fish. Res. Board Can. 35, 1290-1296; Wedemeyer et al. (1979) J. Fish. Res Board Can. 36, 605-614; Liltved et al. (1995) Aquac. Eng. 14, 107-114; Meunpol et al. (2003) Aquaculture 220, 437-448). It is also known that certain microorganisms can be destroyed by short-wave light, such as UV light. A combination of ozone and UV light application is taken up, for example, in US Pat. No. 5,961,831 in a description of a closed aquaculture filter system. However, the poor availability of powerful ozone generators, the frequent use of open or semi-open aquaculture systems and the high costs for this system limit the practical usability. In addition, the use of ozone and UV light also kills microorganisms with a positive effect.

Another path that has been taken to control pathogenic microorganisms in animal production and in humans is the use of so-called probiotics. The latter are defined as living microorganisms which, when ingested by an animal or by humans, have beneficial effects on the target organism in question. The positive effects of probiotics are conveyed, for example, by immunostimulatory effects or anti-microbial properties. Furthermore, it is assumed that probiotics are able to displace pathogenic microorganisms through competitive exclusion, for example through competition for food sources or settlement areas. A further discrimination of probiotics into endo-probiotics and exo-probiotics can be made: the former have positive effects after being absorbed by the target organism, for example by settling and acting in the gastrointestinal tract of the target organism or stimulating special specific or non-specific immune responses, the latter causing these effects by settling in the environment of the target organism.

Also in the field of aquaculture, numerous studies and developments related to the use of probiotic systems have been made in recent years. For example, the influence of Pseudomonas sp. on Vibrio sp. investigated (Gram et al. (1999) Appl. Environ. Microbiol. 65, 969-973). As a target organism, i.e. the organism on which the probiotics should have a positive influence was chosen in this study Oncorynchus mykiss (rainbow trout). Further studies with fluorescent pseudo-months to control the fish pathogens Saprolegnia sp. (Bly et al. (1997) J. Fish Dis. 20, 35-40) or Aeromonas salmonicida (Smith et al (1993) J. Fish Dis. 16, 521-524) are known. The mechanisms underlying the control of the pathogens are attributed to the endogenous production of antibiotics, hydrocyanic acid or siderophores by the pseudomonads.

The influence of various commercial endo-probiotic mixtures on the ecological balance in the production of Cyprinus carpio (carp) was also investigated (Niiro et al. (2001) Suisanzoshoku 49, 91-96). The probiotic mixtures used contained Bacillus spp. Lactojacillus spp., Streptococcus spp. Saccharomyces spp., Candida utilis, - Clostridium spp. and Bifidobacterium spp.

A Lactococcus sp., Obtained from the gastrointestinal tract of the flounder, could have an inhibitory effect on a pathogenic Streptococcus sp. (Jeong et al. (2003) J. Fish. Sei. Tech. 6, 27-33). A particular focus in the investigation and development of probiotic systems is on Penaeus spp. (Shrimp) as target organisms. In this context, Bacillus spp. and Lactobacillus spp. examined as probiotics. For example, Dalmin et al. the application of Bacillus spp. in the production of Penaeus monodon, which are particularly useful for reducing pathogenic Vijbrio spp. contribute and also have a positive influence on water quality (Dalmin et al. (2001) Indian J. Exp. Biol. 39, 939-942). Vaseeharan et al. use cell-free extract or cultures of Bacillus subtilis BT23 in a similar study and have a controlling effect on pathogenic Vibrio spp. in vitro and in vivo (Vaseeharan et al. (2003) Lett. Appl. Microbiol. 36, 83-87). The influence of Bacillus Sll on Penaei infected with Vibrio harveyi has also been described by Meunpol et al. examined (Meunpol et al. (2003) Aquaculture 220, 437-448). Another study by the Renpipat group describes the positive effect of a mixture of Lactobacillus spp. in the pathogen / target organism system described above (Phianphak et al. (1999) J. Sei. Res. Chula. Univ. 24, 41-51).

WO 97/06811 describes the use of a bacteriostatic or a bacteriocidal Carnojbacterium strain for the treatment of mammals including humans, fish, shellfish and mussels.

WO 02/00035, on the other hand, describes a method for controlling or preventing disease in aquatic organisms by feeding the organisms with a composition according to the invention which contains at least one type of probiotic bacteria and at least one inhibitory or regulatory component, in particular furanones. Finally, US Pat. No. 6,562,585 describes a mixture and a method for aerobic oxidation and reduction of organic and inorganic N ₂ loads. The bacterial composition according to the invention is selected from and limited to Bacillus spp. for the cleaning of contaminated water, waste water flows and waste water systems. The cleaning takes place in an aerobic process. In addition to the use of bacteria, the use of enzymes such as cellulases, amylases, proteases and lipases is also described.

There is therefore a growing need for methods or compositions by means of which diseases of aquatic organisms can be combated particularly effectively, in particular without the above-mentioned undesirable side effects.

The object of the present invention was therefore to provide a method and a composition which enable efficient control of important pathogens of aquatic organisms or the improvement of the health of these organisms and which avoids the disadvantages of the prior art.

This object is achieved by the method according to claim 1. It has surprisingly been found that the use of at least one Brevibacillus parabrevis bacterium enables a particularly positive health-promoting effect in aquatic organisms and an efficient control of important pathogens in aquatic organisms. It has unexpectedly been shown that Brevibacillus parabrevis has a significantly greater reduction, e.g. of the lead pathogen Vibrio harveyi than is the case with known probiotic microorganisms.

The method according to the invention can initially be used for the (therapeutic) control or control of an aquatic disease Organisms, in particular through an infestation with pathogenic microorganisms, are used. However, preventive use is also included, ie the composition containing Brevibacillus parabrevis can also be used in "healthy" aquatic organisms, ie before a disease or an infestation with pathogens or before disease symptoms become visible. In many cases, such a prophylactic or preventive use of the method according to the invention can be useful in order to exclude or minimize the spread of pathogenic organisms from the outset.

The diseases to be treated are in particular diseases which are at least partially caused by microorganisms, in particular bacteria or viruses. Also included are diseases in which at least one or more symptoms are directly or indirectly due to the presence of such microorganisms or their metabolites.

Typical disease-causing microorganisms are found in aquatic organisms such as crustacea or Penaeus spec. (Shrimp) gram-negative bacteria, especially Vibrio spec. , In addition to the most widespread leading pathogen Vibrio harveyi, examples include V. parahaemolyticus, V. splendidus, V. mimicus, V. cholerae, V. alginolyticus, V. anguillarum. Furthermore, species of the bacterial genus Aeromonas are known as pathogens of aquatic organisms.

Numerous disease-causing microorganisms are also known among the so-called gram-positive bacteria, in particular from the bacterial genera Streptococcus, Carnobacterium, Micrococcus. Such pathogens are familiar to the person skilled in the art and therefore need not be characterized in more detail here. The method according to the invention is preferably used in aquatic organisms, but use in land organisms is also not excluded. In practice, commercial aquacultures, in particular in basins, containers, tubs, cages, aquariums, tanks, ponds or the like, will often be in the foreground. Of course, the method according to the invention is also useful in the private keeping or breeding of aquatic organisms.

Preferred aquatic organisms, in which the method according to the invention can be carried out, include, without limitation, crustaceans, fish, aquatic plants, mollusks and in particular Penaeus spec. (Shrimp).

The composition used in the process according to the invention contains at least one bacterium of the brevibacillus parabrevis type. It is believed, without restricting the invention to the assumption of this theoretical mechanism of action, that Brevibacillus parabrevis acts as a particularly efficient probiotic agent. The health-promoting effect on the aquatic organisms could be based on at least one of the following mechanisms, in particular on the interaction of several of the following mechanisms: a) control of pathogenic organisms by competitive exclusion, such as competition for feed and habitat; b) direct inhibition by production of bactericidal or bacteriostatic compounds against pathogenic microorganisms; c) inhibition of the expression of virulence genes or harmful metabolites in or by the pathogenic microorganisms; d) degradation of harmful metabolic products of the pathogenic microorganisms, for example by absorption or degradation of such harmful metabolic products by secreted or cellular enzymes; e) positive influence on water quality, in particular by stabilizing the sludge formation and reducing the nitrogen load of the culture medium. f) Positive influence on the immune system and the immune system of aquatic organisms, especially after absorption in the digestive tract.

According to the invention, living cells from Brevibacillus parabrevis are used in particular. In addition, cells are preferably capable of reproduction in order to ensure the effect also with regard to the control of pathogenic organisms by competitive exclusion. Dead or destroyed cells, for example cells destroyed by heat sterilization, lysis or fragmentation or no longer capable of reproduction, would not be suitable for this. The amount or concentration of the cells (cultures) of Brevibacillus parabrevis used according to the invention can vary within wide limits, but will generally preferably be at least 10 ¹ ', preferably at least 10 ² cells per ml of the aquaculture medium to be treated.

According to a preferred embodiment of the invention, Brevibacillus parabrevis can be used together with other probiotic microorganisms, such as, for example, species of the genus Bacillus or Lactobacillus, Vibrio, Cellulomons, Pseudomonas or Bifidobacterium spp. Probiotic bacteria can be selected, for example, from strains of: Bacillus subtilis, Bacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis _r Bacillus azotoformans, Bacillus cir- culans, Bacillus pumilus, Bacillus firmus _r Paenibacillus polymyxa, Paenibacillus macarans or Alteramonas spp., Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei _r Lactobacillus casei subsp. tolerans, Lactobacillus jensenii, Bifidobacterium bifidum, Vibrio alginolyticus, Pseudomonas stutzeri.

In the context of the present invention, it was also unexpectedly found that compositions show particularly good results which, in addition to at least one representative of Brevibacil lus parabrevis, also contain the following additional microorganisms: Pseudomonas spp. _r Cellulomonas biazotea, Bacillus spp. , Rhodopseudomonas pallustris, Nitrosomonas europaea, Nitrobacter winogradskyi.

It has been shown that the compositions according to the invention, as defined in the present description, not only particularly effectively inhibit or combat pathogens of aquatic organisms, but also improve the water quality and stabilize the critical sludge and nitrogen cycles in the water or Culture medium can be achieved. A synergistic interaction with nitrifying organisms such as Nitrosomonas spec. and / or Nitrobacter spec. be determined. It is known that pathogenic organisms, such as Vibrio harveyi as the most common pathogenic bacterium in crabs, find an ideal breeding ground in the sludge sediment of aquacultures. Here, the composition according to the invention brings about a reduction in the pathogens in the sludge sediment and a reduction in the harmful sludge sediment and the nitrogen load itself, in particular in interaction with the above nitrifying agents. By displacing filamentous and free-floating algae often associated with pathogens, eliminating feed residues and the reduction of organic sludge deposits, the water quality is also increased. According to a particularly preferred embodiment of the invention, the following composition has proven to be particularly effective here: at least one representative of Nitrosomonas and Nitrobacter, at least one representative of the Psedomonades, at least one Bacillus spec, at least one cellulose-degrading bacterium, for example Cellulomonas spec, and at least one representative of the phototrophic bacteria, such as, for example, Rhodopseudomonas pallustris.

It has also been found that the use of the composition according to the invention, in particular in aquaculture, with an increase in stocking density, does not impair the growth rate, survival rate and feed turnover rate of the aquatic organisms and the yield can be increased significantly better than without using the composition according to the invention.

Furthermore, any other additional components can be contained in the composition according to the invention, such as, for example, feed ingredients, other veterinary components such as antibiotics, fungicides or the like, vitamins, nutrients, colorants, or the like. Such additives are familiar to the person skilled in the art in this field and can easily be selected depending on the aquatic organism of interest, the form of aquaculture or the desired dosage unit of the composition according to the invention and can also achieve positive effects in addition to i3revibacillus parabrevis.

In general, the composition used according to the invention can be a liquid or solid composition. The shape and (dosage) size of the composition can be chosen as desired, depending on the desired application. Within the scope of the method according to the invention, a di- the aquatic organisms themselves are treated directly, for example by spraying on or applying the composition comprising Brevibacillus parabrevis, or by adding them to the water or culture medium. It is also possible to treat the individual components of aquaculture or the containers, pipes, pumps, tubs, basins, cages, aquariums, tanks, ponds or the like that are used. Here, too, the composition can be used in liquid or solid form or sprayed on, for example. As mentioned above, it is known that many pathogenic organisms in aquacultures are preferably present in the container walls or in existing sediments or sludge fractions. Thus, according to a preferred embodiment of the invention, the treatment with the composition according to the invention is carried out at these locations in aquaculture.

The at least one brevibacillus parabrevis bacterium can be used in any form (including on a solid support material), preferably in the form of a bacterial culture, a bacterial concentrate, an inoculum, or in the form of spores or lyophilisates from Brevibacillus parabrevis. The composition preferably contains viable or reproducible cells or spores of BreviJbacillus parabrevis, also in lyophilized form. If spores are used, induction of the mination, in particular by heat treatment of the composition, before introduction into the aquaculture can be useful. Parts, e.g. Cell fragments or cell extracts from Brevibacillus parabrevis can be used.

Brevibacillus parabrevis as such is known to the person skilled in the art and is commercially available. A particularly suitable isolate (isolate 2) of Brevibacillus parabrevis was published on May 17, 2004 under the deposit number DSM 16410 on the Name of ASA Spezialenzyme GmbH, Wolfenbüttel, DE, and again on December 16, 2004 under the deposit number DSMZ 16986 in the name of Süd-Chemie AG, Munich, DE, in accordance with the Budapest Treaty with DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Mascheroder Weg lb, D-38124 Braunschweig, deposited.

According to a preferred aspect of the present invention, the method according to the invention comprises the following steps:

(a) providing a composition comprising at least one bacterium of the brevibacillus parabrevis type,

(b) bringing the composition from (a) into contact with an aquatic organism, in particular in an aquaculture.

According to a particularly preferred embodiment, the composition is metered directly into the water or the aquaculture medium, if appropriate via an existing water exchange circuit or a pump system.

As mentioned above, according to a further preferred embodiment according to the invention, the composition can be mixed with feed for the aquatic organisms or incorporated therein. Feed and their components are known to those skilled in the art as such. For example, reference may be made here to WO 02/00035 or WO 02/071867, the disclosure content of which in this regard is expressly incorporated into the present description by reference. This enables targeted absorption by the aquatic organisms to be treated. In addition, an effect as a so-called endo-probiotic after absorption by the aquatic organisms is possible, with the strengthening of the organism's immune system seems to play a role.

In principle, the composition according to the invention can be introduced before, during or after the cultivation of the aquatic organisms. In aquacultures, the plants are generally cleaned after the aquatic organisms have been harvested. In many cases, it may make sense to carry out the method according to the invention before a new generation of aquatic organisms is introduced into aquaculture.

A further aspect of the present invention relates to an aquaculture system for the cultivation or cultivation of aquatic organisms, comprising at least one bacterium of the species Brevibacillus parabrevis, preferably in a concentration of more than 10 ² germs / ml, in particular more than 10 ³ / ml of the aquaculture Medium (e.g. water).

Yet another aspect of the present invention relates to a composition, in particular an additive for aquacultures, containing at least one bacterium of the brevibacillus parabrevis type. The statements made above for the composition used in the process according to the invention apply correspondingly to the additive according to the invention.

The additive can also be a veterinary composition or a bioprotective, i.e. a composition promoting the health of the organisms or living beings to be treated.

The invention will now be explained in more detail with reference to the following non-limiting examples: EXAMPLES

1. Laboratory tests to detect the inhibition of the leading germ Vibrio harveyi

The tests were carried out in shake flasks. V. harveyi was always placed in a concentration of 10 ³ germs (K) / ml in the nutrient medium SMW 3 and incubated with different concentrations of the respective bacterial species or combinations at 30 ° C.

All bacteria used were freshly spread on SMW 1 agar before addition to SMW 3 and, based on this, precultivated in SMW 1 liquid medium. The culture media used:

"Sea water: 19 g sea salt in 500 ml deionized water 2nd test results

The results are summarized in Table 1 below.

The results clearly show the reduction in the growth of Vibrio harveyi with the addition of Brevibacillus parabrevis in different bacterial counts. Other Brevibacilles tested (except parabrevis) showed no inhibitory effect or even a growth-promoting effect on the pathogenic germ. The same applies to tested Brevibacterium spec. , It can also be seen that a combination of Brevibacillus parabrevis with other probiotic bacteria shows positive effects. In both cases, Vibrio's growth When adding 10 ³ K / ml, harveyi was reduced by a factor of more than 1,000 compared to the control. In contrast, the probiotic bacteria known from the prior art Bacillus subtilis, Pseudomonas putida and Pseudomonas stutzeri alone show almost no growth-inhibiting effect on Vibrio harveyi.

Claims

1. A method for promoting health, in particular for the control, control and / or prevention of diseases of aquatic organisms, using a composition which contains at least one bacterium of the species Brevibacil lus parabrevis.

2. The method according to claim 1, characterized in that living, preferably reproducible cells of at least one bacterium of the brevibacillus parabrevis type are used.

S. Method according to one of the preceding claims, characterized in that the aquatic organisms are plant or animal organisms, in particular crustaceans, fish, aquatic plants, mollusks and Penaeus spec. (Shrimp).

i. Method according to one of the preceding claims, characterized in that the disease is at least partially caused by microorganisms, in particular bacteria or viruses, or disease symptoms can be attributed to such microorganisms.

i. Method according to one of the preceding claims, characterized in that the disease-causing microorganism is Gram-negative bacteria, in particular Vibrio spec. , eg Vibrio harveyi, V. parahaemolyticus, V. splendidus, V. mimicus, V. cholerae, V. alginolyticus, V. anguillarum) or Aeromonas spec. is.

6. The method according to any one of the preceding claims, characterized in that the disease-causing microorganism is gram-positive bacteria, such as Streptococcus, Staphylococcus, Carnobacterium, Micrococcus and others.

7. The method according to any one of the preceding claims, characterized in that it is carried out on or in an aquaculture, in particular in containers, tubs, basins, cages, aquariums, tanks, ponds or the like, including the water, surfaces and sediments contained therein.

8. The method according to any one of the preceding claims, characterized in that the composition is a liquid or solid composition.

9. The method according to any one of the preceding claims, characterized in that the at least one of the species Brevibacillus parabrevis is immobilized on a solid support in the composition.

10. The method according to any one of the preceding claims, characterized in that the composition contains: a bacterial culture or a bacterial concentrate, a vaccine culture, spores, a lyophilisate, cell fragments or cell extracts from Brevibacillus parabrevis, and mixtures thereof.

11. The method according to any one of the preceding claims, characterized in that the composition contains further components, in particular other probiotics such as probiotic microorganisms, feed components, other veterinary components such as antibiotics or the like, vitamins, nutrients, binders, Enzymes, biopolymers such as alginates, colorants, fungicides or the like.

12. The method according to any one of the preceding claims, characterized by the following process steps:

(a) provision of a composition comprising at least one bacterium of the breviba cillus pa rabrevis type,

13. The method according to any one of the preceding claims, characterized in that the composition is metered directly into the water or aquaculture medium, if appropriate via an existing water exchange circuit.

14. The method according to any one of the preceding claims, characterized in that the composition is sprayed onto the aquaculture or parts or surfaces of the basins or containers, applied or applied in a similar manner.

15. The method according to any one of the preceding claims, characterized in that a solid form is introduced into or onto the water, the pool or container surfaces, or existing sediment or the like.

16. The method according to any one of the preceding claims, characterized in that the composition is introduced together with or as part of feed.

17. The method according to any one of the preceding claims, characterized in that the at least one brevibacillus parabrevis bacterium is isolate 2 according to the deposits DSM 16410 and DSMZ 16986.

18. The method according to any one of the preceding claims, characterized in that the composition is introduced before, during or after the cultivation of the aquatic organisms.

19. The method according to any one of the preceding claims, characterized in that the application or introduction of the composition takes place directly on or in the aquatic organisms.

20. Aquaculture system for the cultivation or cultivation of aquatic organisms, containing Brevibacillus parabrevis, preferably in a concentration of more than 10 ² / ml, preferably more than 10 ³ / ml of the aquaculture medium.

21. Aquaculture system according to claim 20, characterized in that Brevibacillus parabrevis is applied or introduced via a composition according to claims 1 to 19.

22. Aquaculture according to one of claims 20 and 21, comprising a container or basin with a culture medium for cultivating or cultivating the aquatic organisms and optionally aquatic organisms, at least one bacterium of the species Brevibacillus parabrevis in the culture medium, the container, the surfaces thereof, existing sediments or aquatic organisms.

23. Additive for aquacultures, containing at least one bacterium of the species Brevibacillus parabrevis.

24. Additive according to claim 23, characterized in that it is a feed or a feed additive.

25. Additive according to one of claims 23 or 24, characterized in that it is a veterinary composition.

26. Additive according to one of claims 23 to 25, characterized in that it contains a composition as defined in one of claims 1 to 18.

27. Use of Brevibacillus parabrevis for therapeutic and / or prophylactic treatment or control of diseases of aquatic organisms, in particular crustaceans, fish, aquatic plants, mollusks or Penaeus spec ..

28. Use according to claim 27, wherein Brevibacillus parabrevis is used in the form of living, reproducible cells.

29. Use according to claim 27, wherein Brevibacillus parabrevis is contained in a veterinary or probiotic composition, optionally with further veterinary or probiotic components.