WO2005114171A3 - Analyse d'eluats de chromatographie en phase liquide - Google Patents

Analyse d'eluats de chromatographie en phase liquide Download PDF

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Publication number
WO2005114171A3
WO2005114171A3 PCT/IB2005/001772 IB2005001772W WO2005114171A3 WO 2005114171 A3 WO2005114171 A3 WO 2005114171A3 IB 2005001772 W IB2005001772 W IB 2005001772W WO 2005114171 A3 WO2005114171 A3 WO 2005114171A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
eluate
liquid chromatography
analysis
amperometric
Prior art date
Application number
PCT/IB2005/001772
Other languages
English (en)
Other versions
WO2005114171A2 (fr
Inventor
Daniela Proietti
Angela Bardotti
Stefano Ricci
Original Assignee
Chiron Srl
Daniela Proietti
Angela Bardotti
Stefano Ricci
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0411283A external-priority patent/GB0411283D0/en
Priority claimed from GB0420649A external-priority patent/GB0420649D0/en
Priority to BRPI0511442-0A priority Critical patent/BRPI0511442A/pt
Priority to AU2005246093A priority patent/AU2005246093B2/en
Priority to MXPA06013473A priority patent/MXPA06013473A/es
Priority to AT05748363T priority patent/ATE535804T1/de
Application filed by Chiron Srl, Daniela Proietti, Angela Bardotti, Stefano Ricci filed Critical Chiron Srl
Priority to CA002567528A priority patent/CA2567528A1/fr
Priority to EP05748363A priority patent/EP1747457B1/fr
Priority to CN2005800211868A priority patent/CN1973202B/zh
Priority to JP2007517520A priority patent/JP4714739B2/ja
Priority to NZ551448A priority patent/NZ551448A/en
Priority to US11/597,254 priority patent/US7833803B2/en
Publication of WO2005114171A2 publication Critical patent/WO2005114171A2/fr
Publication of WO2005114171A3 publication Critical patent/WO2005114171A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/78—Detectors specially adapted therefor using more than one detector
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/64—Electrical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8836—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving saccharides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

Lors de l'analyse de saccharides par chromatographie d'échange d'anions haute performance (HPAEC), l'éluat issu de la colonne est en général analysé à l'aide d'un détecteur ampérométrique. Selon l'invention, la détection ampérométrique est associée à la détection UV, les deux techniques étant appliquées à l'éluat. L'invention concerne donc un procédé permettant d'analyser l'éluat issu d'une colonne de chromatographie en phase liquide, qui consiste à analyser l'éluat à la fois par (a) détection ampérométrique et (b) détection UV. Les données pouvant être dérivées des deux types de détection dépassent avantageusement celles qui peuvent être obtenues avec seulement l'une des deux techniques.
PCT/IB2005/001772 2004-05-20 2005-05-20 Analyse d'eluats de chromatographie en phase liquide WO2005114171A2 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
US11/597,254 US7833803B2 (en) 2004-05-20 2005-05-20 Analysis of liquid chromatography eluates
NZ551448A NZ551448A (en) 2004-05-20 2005-05-20 Analysis of liquid chromotography eluates
AU2005246093A AU2005246093B2 (en) 2004-05-20 2005-05-20 Analysis of liquid chromatography eluates
MXPA06013473A MXPA06013473A (es) 2004-05-20 2005-05-20 Analisis de fluidos de cromatografia liquida.
AT05748363T ATE535804T1 (de) 2004-05-20 2005-05-20 Analyse flüssigkeitschromatographischer eluate
BRPI0511442-0A BRPI0511442A (pt) 2004-05-20 2005-05-20 análise de eluatos por cromatografia lìquida
CA002567528A CA2567528A1 (fr) 2004-05-20 2005-05-20 Analyse d'eluats de chromatographie en phase liquide
EP05748363A EP1747457B1 (fr) 2004-05-20 2005-05-20 Analyse d'eluats de chromatographie en phase liquide
CN2005800211868A CN1973202B (zh) 2004-05-20 2005-05-20 液体色谱洗出液的分析
JP2007517520A JP4714739B2 (ja) 2004-05-20 2005-05-20 液体クロマトグラフィー溶出液の分析

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0411283A GB0411283D0 (en) 2004-05-20 2004-05-20 Analysis of liquid chromatography eluates
GB0411283.5 2004-05-20
GB0420649.6 2004-09-16
GB0420649A GB0420649D0 (en) 2004-09-16 2004-09-16 Analysis of liquid chromatography eluates

Publications (2)

Publication Number Publication Date
WO2005114171A2 WO2005114171A2 (fr) 2005-12-01
WO2005114171A3 true WO2005114171A3 (fr) 2006-04-13

Family

ID=34979826

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2005/001772 WO2005114171A2 (fr) 2004-05-20 2005-05-20 Analyse d'eluats de chromatographie en phase liquide

Country Status (11)

Country Link
US (1) US7833803B2 (fr)
EP (1) EP1747457B1 (fr)
JP (1) JP4714739B2 (fr)
AT (1) ATE535804T1 (fr)
AU (1) AU2005246093B2 (fr)
BR (1) BRPI0511442A (fr)
CA (1) CA2567528A1 (fr)
MX (1) MXPA06013473A (fr)
NZ (1) NZ551448A (fr)
RU (1) RU2380700C2 (fr)
WO (1) WO2005114171A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0524782D0 (en) * 2005-12-05 2006-01-11 Chiron Srl Analysis of samples
GB0612854D0 (en) 2006-06-28 2006-08-09 Novartis Ag Saccharide analysis
EP2820408A4 (fr) 2012-02-27 2015-11-18 Del Mar Pharmaceuticals Procédés analytiques améliorés pour analyser et déterminer des impuretés dans le dianhydrogalactitol
CN102645504B (zh) * 2012-05-10 2014-04-30 江南大学 一种灵芝孢子粉多糖离子色谱指纹图谱的构建方法
US10124051B2 (en) 2012-05-22 2018-11-13 Glaxosmithkline Biologicals Sa Meningococcus serogroup X conjugate
JP6214999B2 (ja) * 2013-10-21 2017-10-18 住友ベークライト株式会社 電気化学的検出のための糖鎖試料の調製方法
EP3071555A4 (fr) * 2013-11-18 2017-05-31 Del Mar Pharmaceuticals Analyse par clhp des impuretés contenues dans du dianhydrogalactitol
EP2944955A1 (fr) * 2014-05-13 2015-11-18 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Étalon pour systèmes LC-MS
KR102204927B1 (ko) * 2016-07-25 2021-01-19 에스케이바이오사이언스(주) 피막 다당류-단백질 접합 백신의 품질 평가 방법
US10877005B2 (en) * 2016-12-20 2020-12-29 Dionex Corporation Method of measuring an analyte with a reductive detection waveform

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1151922A (ja) * 1997-08-04 1999-02-26 Hitachi Ltd 液体クロマトグラフ
US20030062475A1 (en) * 2001-09-20 2003-04-03 Esa, Inc. Coupling electrochemistry to mass spectrometry and high performance liquid chromatography

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1220053A (fr) * 1983-10-01 1987-04-07 Toyo Soda Manufacturing Co., Ltd. Methode et appareil d'analyse par chromatographie
FR2737730B1 (fr) * 1995-08-10 1997-09-05 Pasteur Merieux Serums Vacc Procede de purification de virus par chromatographie
JPH0980039A (ja) * 1995-09-14 1997-03-28 Shiseido Co Ltd 電気化学検出器を有する液体クロマトグラフィ装置
MXPA04009339A (es) * 2002-03-26 2005-01-25 Chiron Srl Sacaridos modificados que tienen estabilidad mejorada en agua.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1151922A (ja) * 1997-08-04 1999-02-26 Hitachi Ltd 液体クロマトグラフ
US20030062475A1 (en) * 2001-09-20 2003-04-03 Esa, Inc. Coupling electrochemistry to mass spectrometry and high performance liquid chromatography

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BARDOTTI A A ET AL: "Quantitative determination of saccharide in Haemophilus influenzae type b glycoconjugate vaccines, alone and in combination with DPT, by use of high-performance anion-exchange chromatography with pulsed amperometric detection", VACCINE, BUTTERWORTH SCIENTIFIC. GUILDFORD, GB, vol. 18, no. 19, April 2000 (2000-04-01), pages 1982 - 1993, XP004202493, ISSN: 0264-410X *
CATALDI TOMMASO R I ET AL: "Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection: The potential is still growing", FRESENIUS' JOURNAL OF ANALYTICAL CHEMISTRY, vol. 368, no. 8, December 2000 (2000-12-01), pages 739 - 758, XP002347664, ISSN: 0937-0633 *
KOTANI NOIHIRO ET AL: "Analysis of 2-aminobenzamide-labeled oligosaccharides by high-pH anion-exchange chromatography with fluorometric detection", ANALYTICAL BIOCHEMISTRY, vol. 264, no. 1, 1 November 1998 (1998-11-01), pages 66 - 73, XP002347663, ISSN: 0003-2697 *
OBREZKOV O N ET AL: "ION CHROMATOGRAPHIC DETERMINATION OF ANIONS, ESPECIALLY SULPHUR- CONTAINING ANIONS, WITH CONDUCTIMETRIC AND KINETIC DETECTION", JOURNAL OF CHROMATOGRAPHY, ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, NL, vol. 558, no. 1, 1 September 1991 (1991-09-01), pages 209 - 213, XP000223257, ISSN: 0021-9673 *
PATENT ABSTRACTS OF JAPAN vol. 1999, no. 05 31 May 1999 (1999-05-31) *

Also Published As

Publication number Publication date
EP1747457B1 (fr) 2011-11-30
AU2005246093B2 (en) 2011-09-29
US7833803B2 (en) 2010-11-16
BRPI0511442A (pt) 2007-12-26
AU2005246093A1 (en) 2005-12-01
JP4714739B2 (ja) 2011-06-29
NZ551448A (en) 2011-02-25
RU2006144969A (ru) 2008-06-27
RU2380700C2 (ru) 2010-01-27
JP2007538251A (ja) 2007-12-27
MXPA06013473A (es) 2007-03-01
US20080258710A1 (en) 2008-10-23
WO2005114171A2 (fr) 2005-12-01
ATE535804T1 (de) 2011-12-15
EP1747457A2 (fr) 2007-01-31
CA2567528A1 (fr) 2005-12-01

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