Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
  • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
  • C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
  • C07H17/08—Hetero rings containing eight or more ring members, e.g. erythromycins
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/04—Antibacterial agents

Definitions

• the present invention relates to novel semi-synthetic macrolides having antimicrobial activity, in particular antibacterial activity. More particularly, the invention relates to 14- and 15-membered macrolides substituted at the 4" position, to processes for their preparation, to compositions containing them and to their use in medicine.
• Macrolide antibacterial agents are known to be useful in the treatment or prevention of bacterial infections.
• the emergence of macrolide-resistant bacterial strains has resulted in the need to develop new macrolide compounds.
• EP 0 895 999 describes derivatives modified at the 4" position of the macrolide ring having antibacterial activity.
• Rl is -OC(O)(CH 2 )dXR ⁇ ;
• R2 is hydrogen or a hydroxyl protecting group;
• R3 is hydrogen, C ⁇ _4alkyl, or C3_galkenyl optionally substituted by 9 to 10 membered fused bicyclic heteroaryl;
• R4 is hydroxy, C2_6alkenyloxy optionally substituted by 9 to 10 membered fused bicyclic heteroaryl, or Cj _galkoxy optionally substituted by C1.galkoxy or -O(CH2) e
• R 7 R ⁇ 3 R5 is hydroxy, or
• Y is a bivalent radical selected from -CH/)-, -CH(CN)-, -O-, -N(R ⁇ )- and
• R" is hydrogen or fluorine
• R 7 is hydrogen or Ci ..galkyl
• R 10 is -OR 17 , C ⁇ _ 6 alkyl, -(CH2) g aryl, -(CH 2 ) g heterocyclyl or -(CH ⁇ OCCH ⁇ iOR 7 wherein each R 0 group is optionally substituted by up to three groups independently selected from R ⁇ ;
• R 1 1 is a heterocyclic group having the following structure:
• Rl2 is hydrogen or Ci _galkyl
• Rl3 is hydrogen or C ⁇ _4alkyl substituted by a group selected from optionally substituted phenyl, optionally substituted 5 or 6 membered heteroaryl and optionally substituted 9 to 10 membered fused bicyclic heteroaryl;
• Rl4 and R 5 are each independently hydrogen or C j .galkyl
• Rl6 is halogen, cyano, nitro, trifluoromethyl, azido, -C(O)R 21 , -C(O)OR 21 , -OC(O)R 21 , - OC(O)OR 21 , -NR 22 C(O)R 23 , -C(O)NR 22 R 2 3, -NR 2 R 23 , hydroxy, C galkyl, -S(O)kC ⁇ _ galkyl, Cj.galkoxy, -(CH ) m aryl or -(CH2)mh eteroar ⁇ wherein the alkoxy group is optionally substituted by up to three groups independently selected from -NR1 R15 3 halogen and -OR ⁇ , and the aryl and heteroaryl groups are optionally substituted by up to five groups independently selected from halogen, cyano, nitro, trifluoromethyl, azido, -C(O)R 2 4 3 - C(O)OR 2 , -
• R! 7 is hydrogen, C ⁇ _5alkyl, C3_7cycloalkyl, C3_galkenyl or a 5 or 6 membered heterocyclic group, wherein the alkyl, cycloalkyl, alkenyl and heterocyclic groups are optionally substituted by up to three substituents independently selected from optionally substituted 5 or 6 membered heterocyclic group, optionally substituted 5 or 6 membered heteroaryl, -OR 27 , -
• R 18 is hydrogen, -C(O)OR 29 , -C(O)NHR 29 5 -C(O)CH 2 NO2 3 or -C(O)CH 2 SO 2 R 7 ;
• R* 9 is hydrogen, C ⁇ _4alkyl optionally substituted by hydroxy, cyano, NH 2 , -NH(C ⁇ _4alkyl) or -N(C ⁇ _4alkyl) ; C 2 _4alkenyl optionally substituted by hydroxy, cyano, NH 2 , -NH(C ⁇ _ 4 alkyl) or -N(C ⁇ _ 4 alkyl) 2 ; C ⁇ _ 4 alkoxy, C 3 _7cycloalkyl, -NH 2 , -NH(C ⁇ _ 4 alkyl) or -N(C ⁇ _ 4alkyl) 2 ; (C ⁇ _4alkyl)OC(O)N(C ⁇ -4 alkyl) or optionally substituted phenyl or benzyl;
• R 20 is halogen, C ⁇ alkyl, C ⁇ thioalkyl, C ⁇ _ alkoxy, -NH 2 , -NH(C ⁇ _4alkyl) or -N(C ⁇ _ 4alkyl) 2 ;
• R 2 1 is hydrogen, Ci _ ⁇ oalkyl, -(CH 2 )paryl or -(CH 2 )pheteroaryl;
• R 22 and R 23 are each independently hydrogen, -OR ⁇ Ci.galkyl, -(CH 2 )qaryl or - (CH )qheterocyclyl;
• R 2 is hydrogen, C ⁇ _ ⁇ alkyl, -(CH ⁇ j ryl or -(CH 2 ) r heteroaryl;
• R 2 ⁇ and R 2 ⁇ are each independently hydrogen, -ORl 5 C ⁇ aUcyl, -(CH 2 ) s aryl or - (CH ) s heterocyclyl;
• R 27 and R 28 are each independently hydrogen, Ci _4alkyl or
• R 29 is hydrogen or C ⁇ _6al yl optionally substituted by up to three groups independently selected from halogen, C ⁇ _4alkoxy, -OC(O)C ⁇ _6alkyl and -OC(O)OC ⁇ galkyl, - (CH )qheterocyclyl, -(CH 2 ) q heteroaryl, -(CH2) q aryl, or -(CH 2 ) q C 3-7 cycloalkyl; R30 is hydrogen, C ⁇ _4alkyl, C3_7cycloalkyl, optionally substituted phenyl or benzyl, acetyl orbenzoyl; R 1 is hydrogen or R 2 ⁇ , or R31 and R i9 are linked to form the bivalent radical -O(CH 2 ) 2 -, -(CH 2 )t-;-NR 7 (CH 2 ) a -, -OCH 2 NR 7 ⁇ , -SCH 2 NR 7 -, -CH
• U, B, D and E are independently divalent radicals selected from -N(R30)-, -0-, -S(O) z -, N(R 30 )C(O)-, -C(O)N(R30)- and -N[C(O)R30].
• W is -C(R 1)- or a nitrogen atom; • a is l or 2 b is an integer from 1 to 3; d is an integer from 1 to 5; e is an integer from 2 to 4; f, g, h, m, p, q, r and s are each independently integers from 0 to 4; i is an integer from 1 to 6; j, k, n and z are each independently integers from 0 to 2; t is 2 or 3; v is an integer from 1 to 8 independantly selected for each occurance;
• salts and solvates of compounds of the invention which are suitable for use in medicine are those wherein the counterion or associated solvent is pharmaceutically acceptable.
• salts and solvates having non-pharmaceutically acceptable counterions or associated solvents are within the scope of the present invention, for example, for use as intermediates in the preparation of other compounds of the invention and their pharmaceutically acceptable salts and solvates.
• pharmaceutically acceptable derivative means any pharmaceutically acceptable salt, solvate or prodrug, e.g. ester, of a compound of the invention, which upon administration to the recipient is capable of providing (directly or indirectly) a compound of the invention, or an active metabolite or residue thereof.
• Such derivatives are recognizable to those skilled in the art, without undue experimentation. Nevertheless, reference is made to the teaching of Burger's Medicinal Chemistry and Drug Discovery, 5 th Edition, Vol 1: Principles and Practice, which is incorporated herein by reference to the extent of teaching such derivatives.
• Preferred pharmaceutically acceptable derivatives are salts, solvates, esters, carbamates and phosphate esters. Particularly preferred pharmaceutically acceptable derivatives are salts, solvates and esters. Most preferred pharmaceutically acceptable derivatives are salts and esters.
• the compounds of the present invention may be in the form of and/or may be administered as a pharmaceutically acceptable salt.
• suitable salts see Berge et ah, J. Pharm. Sci., 1977, 66, 1-19.
• a pharmaceutical acceptable salt may be readily prepared by using a desired acid or base as appropriate.
• the salt may precipitate from solution and be collected by filtration or may be recovered by evaporation of the solvent.
• an aqueous solution of an acid such as hydrochloric acid may be added to an aqueous suspension of a compound of formula (I) and the resulting mixture evaporated to dryness (lyophilised) to obtain the acid addition salt as a solid.
• a compound of formula (I) may be dissolved in a suitable solvent, for example an alcohol such as isopropanol, and the acid may be added in the same solvent or another suitable solvent.
• the resulting acid addition salt may then be precipitated directly, or by addition of a less polar solvent such as diisopropyl ether or hexane, and isolated by filtration.
• Suitable addition salts are formed from inorganic or organic acids which form non-toxic salts and examples are hydrochloride, hydrobromide, hydroiodide, sulphate, bisulphate, nitrate, phosphate, hydrogen phosphate, acetate, trifluoroacetate, maleate, malate, fumarate, lactate, tartrate, citrate, formate, gluconate, succinate, pyruvate, oxalate, oxaloacetate, trifluoroacetate, saccharate, benzoate, alkyl or aryl sulphonates (eg methanesulphonate, ethanesulphonate, benzenesulphonate or p-toluenesulphonate) and isethionate.
• Representative examples include trifluoroacetate and formate salts, for example the bis or tris trifluoroacetate salts and the mono or diformate salts, in particular the tris or bis trifluoroa
• Pharmaceutically acceptable base salts include ammonium salts, alkali metal salts such as those of sodium and potassium, alkaline earth metal salts such as those of calcium and magnesium and salts with organic bases, including salts of primary, secondary and tertiary amines, such as isopropylamine, diethylamine, ethanolamine, trimemylamine, dicyclohexyl amine and N-methyl-D-glucamine.
• Compounds of the invention may have both a basic and an acidic centre may therefore be in the form of zwitterions.
• solvates complexes with solvents in which they are reacted or from which they are precipitated or crystallized. These complexes are known as "solvates". For example, a complex with water is known as a "hydrate”. Solvates of the compound of the invention are within the scope of the invention.
• the salts of the compound of formula (I) may form solvates (e.g. hydrates) and the invention also includes all such solvates.
• prodrug as used herein means a compound which is converted within the body, e.g. by hydrolysis in the blood, into its active form that has medical effects. Pharmaceutically acceptable prodrugs are described in T. Higuchi and V.
• Prodrugs are any covalently bonded carriers that release a compound of structure (I) in vivo when such prodrug is administered to a patient.
• Prodrugs are generally prepared by modifying functional groups in a way such that the modification is cleaved, either by routine manipulation or in vivo, yielding the parent compound.
• Prodrugs include, for example, compounds of this invention wherein hydroxy, amine or sulfhydryl groups are bonded to any group that, when administered to a patient, cleaves to form the hydroxy, amine or sulfhydryl groups.
• prodrugs include (but are not limited to) acetate, formate and benzoate derivatives of alcohol, sulfhydryl and amine functional groups of the compounds of structure (I).
• esters may be employed, such as methyl esters, ethyl esters, and the like. Esters may be active in their own right and/or be hydrolysable under in vivo conditions in the human body. Suitable pharmaceutically acceptable in vivo hydrolysable ester groups include those which break down readily in the human body to leave the parent acid or its salt.
• references hereinafter to a compound according to the invention include both compounds of formula (I) and their pharmaceutically acceptable derivatives.
• the compounds of structure (I) have more than one asymmetric carbon atom.
• the solid wedge shaped bond indicates that the bond is above the plane of the paper.
• the broken bond indicates that the bond is below the plane of the paper.
• the substituents on the macrolide may also have one or more asymmetric carbon atoms.
• the compounds of structure (I) may occur, as individual enantiomers or diastereomers. All such isomeric forms are included within the present invention, including mixtures thereof.
• a compound of the invention contains an alkenyl group
• cis (Z) and trans (E) isomerism may also occur.
• the present invention includes the individual stereoisomers of the compound of the invention and, where appropriate, the individual tautomeric forms thereof, together with mixtures thereof.
• Separation of diastereoisomers or cis and trans isomers may be achieved by conventional techniques, e.g. by fractional crystallisation, chromatography or HPLC.
• a stereoisomeric mixture of the agent may also be prepared from a corresponding optically pure intermediate or by resolution, such as HPLC of the corresponding mixture using a suitable chiral support or by fractional crystallisation of the diastereoisomeric salts formed by reaction of the corresponding mixture with a suitable optically active acid or base, as appropriate.
• the compounds of structure (I) may be in crystalline or amorphous form. Furthermore, some of the crystalline forms of the compounds of structure (I) may exist as polymorphs, which are included in the present invention.
• group OR 2 When the group OR 2 is a protected hydroxyl group this is conveniently an ether or an acyloxy group.
• ether groups include those in which R 2 is a trialkylsilyl (i.e. trimethylsilyl).
• R 2 When the group OR 2 represents an acyloxy group, then examples of suitable groups R 2 include acetyl orbenzoyl.
• R6 is hydrogen or fluorine. However, it will be appreciated that when A is -C(O)NH- or - CH 2 -N(R 7 )-, R 6 is hydrogen.
• said heterocyclic is linked in the 6 or 7 position to the X group as above defined.
• the R 2 ⁇ group or groups may be attached at any position on the ring.
• an RTM group is attached at the 6 or 7 position.
• R 1 1 is a heterocyclic group having the following structure:
• W is -C(R31)- wherein R31 is R ⁇ or R31 and R! 9 are linked to form the bivalent radical -O(CH 2 ) 2 -, -(CH2) t -;-NR 7 (CH 2 ) a -, -OCH 2 NR 7 -, -SCH 2 NR 7 -, -CH 2 NR 7 CH 2 -, - 7 CH2OCH 2 -, -CH2SCH 2 -, -(CH2) a NR -, said heterocyclic is linked in the (ii) or (iii) position to the X group as above defined.
• R! 1 is a heterocyclic group having the following structure:
• R 1 ! is a heterocyclic group having the following structure:
• heterocyclic is linked in the 7 or 8 position to the X group as above defined.
• RU is a heterocyclic group having the following structure:
• W is -C(R 1)- wherein R31 is R 2 ⁇ or R31 and R ⁇ 9 are linked to form the bivalent radical -O(CH 2 ) 2 -, -(CH 2 ) t -;-NR 7 (CH 2 ) a -, -OCH 2 NR 7 -, -SCH 2 NR 7 -, -CH 2 NR 7 CH 2 -, - CH 2 OCH 2 -, -CH 2 SCH 2 -, -(CH 2 ) a NR 7 -, said heterocyclic is linked in the (i), (ii) or (iii) position to the X group as above defined. In one embodiment, the heterocyclic is linked to the 15. (i) position. In another embodiment, the heterocyclic is linked in the (ii) or (iii) position.
• R 1 1 is a heterocyclic group having the following structure:
• heterocyclic is linked in the 2 or 3 position to the X group as above defined.
• the heterocyclic is linked in the 2 or 3 position.
• the heterocyclic is linked in the 4 position.
• alkyl as used herein as a group or a part of a group refers to a straight or branched hydrocarbon chain containing the specified number of carbon atoms.
• Ci. i Q alkyl means a straight or branched alkyl containing at least 1, and at most 10, carbon atoms.
• alkyl as used herein include, but are not limited to, methyl, ethyl, n- propyl, n-butyl, n-pentyl, isobutyl, isopropyl, t-butyl, hexyl, heptyl, octyl, nonyl and decyl.
• a Ci _4alkyl group is preferred, for example methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl or t-butyl.
• C3_7cycloalkyl group refers to a non-aromatic monocyclic hydrocarbon ring of 3 to 7 carbon atoms such as, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl.
• alkoxy refers to a straight or branched chain alkoxy group containing the specified number of carbon atoms.
• C ⁇ .galkoxy means a straight or branched alkoxy containing at least 1, and at most 6, carbon atoms.
• alkoxy as used herein include, but are not limited to, methoxy, ethoxy, propoxy, prop-2-oxy, butoxy, but-2-oxy, 2-methylprop-l-oxy, 2-methylprop-2-oxy, pentoxy and hexyloxy.
• a C ⁇ _4alkoxy group is preferred, for example methoxy, ethoxy, propoxy, prop-2-oxy, butoxy, but-2-oxy or 2-methylprop-2-oxy.
• alkenyl as used herein as a group or a part of a group refers to a straight or branched hydrocarbon chain containing the specified number of carbon atoms and containing at least one double bond.
• C _galkenyl means a straight or branched alkenyl containing at least 2, and at most 6, carbon atoms and containing at least one double bond.
• alkenyl examples include, but are not limited to, ethenyl, 2- propenyl, 3-butenyl, 2-butenyl, 2-pentenyh 3-pentenyl, 3-methyl-2-butenyl, 3-methylbut-2- enyl, 3-hexenyl and l,l-dimethylbut-2-enyl. It will be appreciated that in groups of the form - O-C 2 _6alkenyl, the double bond is preferably not adjacent to the oxygen.
• alkynyl as used herein as a group or a part of a group refers to a straight or branched hydrocarbon chain containing the specified number of carbon atoms and containing at least one triple bond.
• C 2 _5alkynyl means a straight or branched alkynyl containing at least 2, and at most 6, carbon atoms and containing at least one triple bond.
• alkynyl as used herein include, but are not limited to, ethynyl, 2-propynyl, 3-butynyl, 2-butynyl, 2-pentynyl, 3-pentynyl, 3-methyl-2-butynyl, 3- methylbut-2-ynyl, 3-hexynyl and l,l-dimethylbut-2-ynyl.
• the triple bond is preferably, not adjacent to the oxygen.
• aryl refers to an aromatic carbocyclic moiety such as phenyl, biphenyl or naphthyl.
• heteroaryl refers to an aromatic heterocycle of 5 to 10 members, having at least one heteroatom selected from nitrogen, oxygen and sulfur, and containing at least 1 carbon atom, including both mono and bicyclic ring systems.
• heteroaryl rings include, but are not limited to, furanyl, thiophenyl, pyrrolyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, tetrazolyl, thiadiazolyl, pyridyl, pyridazinyl, pyrazinyl, pyrimidinyl, triazinyl, quinolinyl, isoquinolinyl, 1,2,3,4-tetrahydroisoquinolinyl, benzofuranyl, benzimidazolyl, benzothienyl, benzoxazolyl, 1,3-benzodioxazolyl, indolyl, benzo thiazolyl, furylpyridine, oxazolopyridyl and benzothiophenyl.
• 5 or 6 membered heteroaryl refers to a monocyclic 5 or 6 membered aromatic heterocycle containing at least one heteroatom independently selected from oxygen, nitrogen and sulfur.
• examples include, but are not limited to, furanyl, thiophenyl, pyrrolyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, oxadiazolyl, tetrazolyl, pyridyl, pyridazinyl, pyrazinyl, pyrimidinyl and triazinyl.
• 9 to 10 membered fused bicyclic heteroaryl refers to quinolinyl, isoquinolinyl, 1,2,3,4-tetrahydroisoquinolinyl, benzofuranyl, benzimidazolyl, benzothienyl, benzoxazolyl, 1,3-benzodioxazolyl, indolyl, benzothiazolyl, furylpyridine, oxazolopyridyl or benzothiophenyl.
• heterocyclyl refers to a monocyclic or bicyclic three- to ten-membered saturated or non-aromatic, unsaturated hydrocarbon ring containing at least one heteroatom selected from oxygen, nitrogen and sulfur.
• the heterocyclyl ring has five or six ring atoms.
• heterocyclyl groups include, but are not Umited to, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothiophenyl, imidazolidinyl, pyrazolidinyl, piperidyl, piperazinyl, morpholino, tetrahydropyranyl and thiomo ⁇ holino.
• heterocyclic group refers to a monocyclic 5 or 6 membered saturated hydrocarbon ring containing at least one heteroatom independently selected from oxygen, nitrogen and sulfur.
• heterocyclyl groups include, but are not limited to, pyrrolidinyl, tetrahydrofuranyl, tetrahydrothiophenyl, imidazolidinyl, pyrazolidinyl, piperidyl, piperazinyl, morpholino, tetrahydropyranyl and thiomo ⁇ holino.
• halogen refers to a fluorine, chlorine, bromine or iodine atom.
• phenyl optionally substituted phenyl
• phenyl or benzyl optionally substituted 5 or 6 membered heteroaryl
• optionally substituted 9 to 10 membered fused bicyclic heteroaryl optionally substituted 5 or 6 membered heterocyclic group
• group which is substituted by 1 to 3 groups selected from halogen, Ci _4alkyl, Ci _4alkoxy, hydroxy, nitro, cyano, amino, Ci _4alkylanu.n0 or diCi _4alkylamino, phenyl and 5 or 6 membered heteroaryl.
• A is -C(O)-, -C(O)NH-, -NHC(O)-, -N(R 7 )-CH 2 -, -CH 2 -N(R 7 )- or -
• A is -C(O)-, -C(O)NH-, -NHC(O)-, -CH 2 -N(R 7 )-, -
• A is -C(O)-, -C(O)NH-, -NHC(O)-, -CH 2 -NR 7 - or -CH(NR 8 R 9 )-.
• Representative examples of A include -C(O)- and -N(R 7 )-
• A is -N(R 7 )-CH 2 -.
• R 2 is hydrogen.
• Representative examples of R include hydrogen and C ⁇ alkyl, in particular hydrogen and methyl.
• R is hydroxy or Ci. ⁇ alkoxy, in particular hydroxy or methoxy.
• R4 is hydroxy.
• ⁇ is hydroxy.
• Y is a bivalent radical selected from -O- and -N(Rl3)_
• R6 is hydrogen
• R 7 is Ci .galkyl, for example Ci _4alkyl, in particular methyl.
• R 1 include heterocyclic groups having the following structures:
• heterocyclic is linked in the 6 or 7 position to the X group as above defined, and heterocyclic groups having the following structure:
• R* * is a heterocyclic group having the following structure:
• R ⁇ 3 is hydrogen.
• R 18 is -C(O)OR 29 , -C(O)NHR 29 > -C(O)CH 2 NO 2 . or -C(O)CH 2 SO 2 R 7 .
• a representative example of R 8 is -C(O)OR 29 .
• R* 8 is -C(O)OR 29 wherein R 29 is hydrogen.
• R* 9 include C ⁇ _4alkyl, in particular ethyl, and C3_7cycloalkyl, in particular cyclopropyl.
• R 2 ⁇ is halogen, in particular chlorine or fluorine, or methoxy.
• R30 is hydrogen or Ci _4alkyl.
• a representative example of R30 is hydrogen or methyl.
• a representative example of R 1 is hydrogen, or R.31 and R 9 are linked to form the bivalent radical -(CH ) t -.
• a representative example of X is -U(CH 2 ) V B(CH 2 ) V D-, -U(CH 2 ) V B(CH 2 ) V D(CH 2 ) V E-, -U(CH 2 ) V B-R 33 -, or U(CH 2 ) V B(CH 2 ) V D-R 33 -.
• U, B, D and E include the divalent radicals -N(R30)-, -0-, S(O) z - , -N(R30)C(O)- and -C(O)N(R 30 )-.
• R 33 is C ⁇ - 8 alkyl or C 2-6 alkynyl. In one preferred embodiment,R 33 is propyl.
• a representative example of d is 1 to 4, for example 2 to 4.
• a particularly preferred example ofd is 2.
• a representative example of v is 1 to 4, for example 2 or 3.
• a particularly preferred example is when each v independently is 2.
• X is -U(CH 2 ) V B(CH 2 ) V D- or -U(CH 2 ) V B-R 33 - wherein U is -O-, B is - O-, and D is -N-.
• Particulalry preferred X groups are O(CH 2 ) 2 O(CH 2 ) 2 N- and -O(CH 2 ) 2 O- (CH 2 ) 3 -
• j is 0 to 2.
• a representative example of j is 0 or 1.
• Particularly preferred compounds of the invention are:
• Compounds according to the invention also exhibit a broad spectrum of antimicrobial activity, in particular antibacterial activity, against a wide range of clinical pathogenic microorganisms. Using a standard microtiter broth serial dilution test, compounds of the invention have been found to exhibit useful levels of activity against a wide range of pathogenic microorganisims.
• the compounds of the invention may be active against strains of Staphylococcus aureus, Streptopococcus pneumoniae, Moraxella catarrhalis, Streptococcus pyogenes, Haemophilus influenzae, Enterococcus faecalis, Chlamydia pneumoniae, Mycoplasma pneumomae and Legionella pneumophila.
• the compounds of the invention may also be active against resistant strains, for example erythromycin resistant strains.
• the compounds of the invention may be active against erythromycin resistant strains of Streptococcus pneumoniae, Streptococcus pyogenes and Staphylococcus aureus.
• the compounds of the invention may therefore be used for treating a variety of diseases caused by pathogenic microorganisms, in particular bacteria, in human beings and animals. It will be appreciated that reference to treatment includes acute treatment or prophylaxis as well as the alleviation of established symptoms.
• a method of treatment of the human or non-human animal body to combat microbial infections comprising adininistration to a body in need of such treatment of an effective amount of a compound of formula (I) or a pharmaceutically acceptable derivative thereof.
• a compound of the invention may be administered as the raw chemical it is preferable to present the active ingredient as a pharmaceutical formulation eg when the agent is in admixture with a suitable pharmaceutical excipient, diluent or carrier selected with regard to the intended route of administration and standard pharmaceutical practice.
• the present invention provides a pharmaceutical composition or formulation comprising at least one compound of the invention or a pharmaceutically acceptable derivative thereof in association with a pharmaceutically acceptable excipient, diluent and/or carrier.
• the excipient, diluent and/or carrier must be "acceptable” in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
• the invention provides a pharmaceutical composition
• a pharmaceutical composition comprising, as active ingredient, at least one compound of the invention or a pharmaceutically acceptable derivative thereof in association with a pharmaceutically acceptable excipient, diluent and/or carrier for use in therapy, and in particular, in the treatment of human or ar ⁇ nal subjects suffering from a condition susceptible to amelioration by an antimicrobial compound.
• the invention provides a pharmaceutical composition
• a pharmaceutical composition comprising a therapeutically effective amount of the compounds of the present invention and a pharmaceutically acceptable excipient, diluent and/or carrier (including combinations thereof).
• a process of preparing a pharmaceutical composition which process comprises mixing at least one compound of the invention or a pharmaceutically acceptable derivative thereof, together with a pharmaceutically acceptable excipient, diluent and/or carrier.
• the compounds of the invention may be formulated for administration in any convenient way for use in human or veterinary medicine and the invention therefore includes within its scope pharmaceutical compositions comprising a compound of the invention adapted for use in human or veterinary medicine.
• Such compositions may be presented for use in a conventional manner with the aid of one or more suitable excipients, diluents and/or carriers.
• compositions may comprise as - or in addition to - the excipient, diluent and/or carrier any suitable binder(s), lubricant(s), suspending agent(s), coating agent(s), solubilising agent(s).
• Preservatives may be provided in the pharmaceutical composition.
• preservatives include sodium benzoate, sorbic acid and esters of p-hydroxybenzoic acid.
• Antioxidants and suspending agents maybe also used.
• the agents of the present invention may also be used in combination with a cyclodextrin.
• Cyclodextrins are known to form inclusion and non-inclusion complexes with drug molecules. Formation of a drug-cyclodextrin complex may modify the solubility, dissolution rate, bioavailability and/or stability property of a drug molecule. Drug- cyclodextrin complexes are generally useful for most dosage forms and administration routes.
• the cyclodextrin may be used as an auxiliary additive, e. g. as a carrier, diluent or solubiliser.
• Alpha-, beta- and gamma- cyclodextrins are most commonly used and suitable examples are described in WO 91/11172, WO 94/02518 and WO 98/55148.
• the compounds of the invention may be milled using known milling procedures such as wet milling to obtain a particle size appropriate for tablet formation and for other formulation types. Finely divided (nanoparticulate) preparations of the compounds of the invention may be prepared by processes known in the art, for example see International Patent Application No. WO 02/00196 (SmithKline Beecham).
• the routes for aclministration (delivery) include, but are not limited to, one or more of: oral (e. g. as a tablet, capsule, or as an ingestable solution), topical, mucosal (e. g. as a nasal spray or aerosol for inhalation), nasal, parenteral (e. g.
• an injectable form by an injectable form), gastrointestinal, intraspinal, intraperitoneal, intramuscular, intravenous, intrauterine, intraocular, intradermal, intracranial, intratracheal, intravaginal, intracerebroventricular, intracerebral, subcutaneous, ophthalmic (including intravitreal or intracameral), transdermal, rectal, buccal, epidural and sublingual.
• the pharmaceutical composition of the present invention may be formulated to be delivered using a mini-pump or by a mucosal route, for example, as a nasal spray or aerosol for inhalation or ingestable solution, or parenterally in which the composition is formulated by an injectable form, for delivery, by, for example, an intravenous, intramuscular or subcutaneous route.
• the formulation may be designed to be delivered by both routes.
• the agent is to be delivered mucosally through the gastrointestinal mucosa, it should be able to remain stable during transit though the gastrointestinal tract; for example, it should be resistant to proteolytic degradation, stable at acid pH and resistant to the detergent effects of bile.
• compositions can be administered by inhalation, in the form of a suppository or pessary, topically in the form of a lotion, solution, cream, ointment or dusting powder, by use of a skin patch, orally in the form of tablets containing excipients such as starch or lactose, or in capsules or ovules either alone or in admixture with excipients, or in the form of elixirs, solutions or suspensions containing flavouring or colouring agents, or they can be injected parenterally, for example intravenously, intramuscularly or subcutaneously.
• compositions may be best used in the form of a sterile aqueous solution, which may contain other substances, for example enough salts or monosaccharides to make the solution isotonic with blood.
• a sterile aqueous solution which may contain other substances, for example enough salts or monosaccharides to make the solution isotonic with blood.
• buccal or sublingual administration the compositions may be administered in the form of tablets or lozenges, which can be formulated in a conventional manner. It is to be understood that not all of the compounds need be administered by the same route. Likewise, if the composition comprises more than one active component, then those components may be administered by different routes.
• compositions of the invention include those in a form especially formulated for parenteral, oral, buccal, rectal, topical, implant, ophthalmic, nasal or genito-urinary use.
• the agents of the present invention are delivered systemically (such as orally, buccally, sublingually), more preferably orally.
• the agent is in a form that is suitable for oral delivery.
• examples of such administration include one or more of: intravenously, intraarterially, intraperitoneally, intrathecally, intraventricularly, intraurethrally, intrasternally, intracranially, intramuscularly or subcutaneously administering the agent; and/or by using infusion techniques.
• the compound is best used in the form of a sterile aqueous solution which may contain other substances, for example, enough salts or glucose to make the solution isotonic with blood.
• aqueous solutions should be suitably buffered (preferably to a pH of from 3 to 9), if necessary.
• suitable parenteral formulations under sterile conditions is readily accomplished by standard pharmaceutical techniques well-known to those skilled in the art.
• the compounds according to the invention may be formulated for use in human or veterinary medicine by injection (e.g. by intravenous bolus injection or infusion or via intramuscular, subcutaneous or intrathecal routes) and may be presented in unit dose form, in ampoules, or other unit-dose containers, or in multi-dose containers, if necessary with an added preservative.
• the compositions for injection may be in the form of suspensions, solutions, or emulsions, in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising, solubilising and/or dispersing agents.
• the active ingredient may be in sterile powder form for reconstitution with a suitable vehicle, e.g. sterile, pyrogen-free water, before use.
• the compounds of the invention can be administered (e. g. orally or topically) in the form of tablets, capsules, ovules, elixirs, solutions or suspensions, which may contain flavouring or colouring agents, for immediate-, delayed-, modified-, sustained-, pulsed-or controlled- release applications.
• the compounds of the invention may also be presented for human or veterinary use in a form suitable for oral or buccal administration, for example in the form of solutions, gels, syrups, mouth washes or suspensions, or a dry powder for constitution with water or other suitable vehicle before use, optionally with flavouring and colouring agents.
• Solid compositions such as tablets, capsules, lozenges, pastilles, pills, boluses, powder, pastes, granules, bullets or premix preparations may also be used.
• Solid and liquid compositions for oral use may be prepared according to methods well known in the art. Such compositions may also contain one or more pharmaceutically acceptable carriers and excipients which may be in solid or liquid form.
• the tablets may contain excipients such as microcrystalline cellulose, lactose, sodium citrate, calcium carbonate, dibasic calcium phosphate and glycine, disintegrants such as starch (preferably corn, potato or tapioca starch), sodium starch glycollate, croscarmellose sodium and certain complex silicates, and granulation binders such as polyvinylpyrrolidone, hydroxypropylmethylcellulose (HPMC), hydroxypropylcellulose (HPC), sucrose, gelatin and acacia.
• excipients such as microcrystalline cellulose, lactose, sodium citrate, calcium carbonate, dibasic calcium phosphate and glycine
• disintegrants such as starch (preferably corn, potato or tapioca starch), sodium starch glycollate, croscarmellose sodium and certain complex silicates
• granulation binders such as polyvinylpyrrolidone, hydroxypropylmethylcellulose (HPMC), hydroxypropylcellulose (HPC),
• lubricating agents such as magnesium stearate, stearic acid, glyceryl behenate and talc may be included.
• compositions of a similar type may also be employed as fillers in gelatin capsules.
• Preferred excipients in this regard include lactose, starch, a cellulose, milk sugar or high molecular weight polyethylene glycols.
• the agent may be combined with various sweetening or flavouring agents, colouring matter or dyes, with emulsifying and/or suspending agents and with diluents such as water, ethanol, propylene glycol and glycerin, and combinations thereof.
• the compounds of the invention may also be administered orally in veterinary medicine in the form of a liquid drench such as a solution, suspension or dispersion of the active ingredient together with a pharmaceutically acceptable carrier or excipient.
• the compounds of the invention may also, for example, be formulated as suppositories e.g. containing conventional suppository bases for use in human or veterinary medicine or as pessaries e.g. containing conventional pessary bases.
• the compounds according to the invention may be formulated for topical administration, for use in human and veterinary medicine, in the form of ointments, creams, gels, hydrogels, lotions, solutions, shampoos, powders (including spray or dusting powders), pessaries, tampons, sprays, dips, aerosols, drops (e.g. eye ear or nose drops) or pour-ons.
• the agent of the present invention can be formulated as a suitable ointment containing the active compound suspended or dissolved in, for example, a mixture with one or more of the following: mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene polyoxypropylene compound, emulsifying wax and water.
• a suitable lotion or cream suspended or dissolved in, for example, a mixture of one or more of the following: mineral oil, sorbitan monostearate, a polyethylene glycol, liquid paraffin, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2- octyldodecanol, benzyl alcohol and water.
• the compounds may also be dermally or transdermally administered, for example, by use of a skin patch.
• the compounds can be formulated as micronised suspensions in isotonic, pH adjusted, sterile saline, or, preferably, as solutions in isotonic, pH adjusted, sterile saline, optionally in combination with a preservative such as a benzylalkonium chloride.
• a preservative such as a benzylalkonium chloride.
• they may be formulated in an ointment such as petrolatum.
• the compound of the present invention can be administered intranasally or by inhalation and is conveniently delivered in the form of a dry powder inhaler or an aerosol spray presentation from a pressurised container, pump, spray or nebuliser with the use of a suitable propellant, e.g. dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, a hydrofluoroalkane such as 1,1,1,2-tetrafluoroethane (HFA 134AT"”) or 1,1,1,2,3,3,3-he ⁇ tafluoropro ⁇ ane (HFA 227EA), carbon dioxide or other suitable gas.
• a suitable propellant e.g. dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, a hydrofluoroalkane such as 1,1,1,2-tetrafluoroethane (HFA 134AT"") or
• the dosage unit may be determined by providing a valve to deliver a metered amount.
• the pressurised container, pump, spray or nebuliser may contain a solution or suspension of the active compound, e. g. using a mixture of ethanol and the propellant as the solvent, which may additionally contain a lubricant, e. g. sorbitan trioleate.
• Capsules and cartridges for use in an inhaler or insufflator may be formulated to contain a powder mix of the compound and a suitable powder base such as lactose or starch.
• the compounds according to the invention may be delivered for use in human or veterinary medicine via a nebuliser.
• the compounds of the invention may also be used in combination with other therapeutic agents.
• the invention thus provides, in a further aspect, a combination comprising a compound of the invention or a pharmaceutically acceptable derivative thereof together with a further therapeutic agent.
• a compound of the invention or a pharmaceutically acceptable derivative thereof When a compound of the invention or a pharmaceutically acceptable derivative thereof is used in combination with a second therapeutic agent active against the same disease state the dose of each compound may differ from that when the compound is used alone. Appropriate doses will be readily appreciated by those skilled in the art. It will be appreciated that the amount of a compound of the invention required for use in treatment will vary with the nature of the condition being treated and the age and the condition of the patient and will be ultimately at the discretion of the attendant physician or veterinarian.
• the compounds of the present invention may for example be used for topical administration with other active ingredients such as corticosteroids or antifungals as appropriate.
• compositions comprising a combination as defined above together with a pharmaceutically acceptable carrier or excipient comprise a further aspect of the invention.
• the individual components of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations by any convenient route.
• either the compound of the invention or the second therapeutic agent may be administered first.
• the combination may be administered either in the same or different pharmaceutical composition.
• the two compounds When combined in the same formulation it will be appreciated that the two compounds must be stable and compatible with each other and the other components of the formulation. When formulated separately they may be provided in any convenient formulation, conveniently in such manner as are known for such compounds in the art.
• compositions may contain from 0.01-99% of the active material.
• the composition will generally contain from 0.01-10%, more preferably 0.01-1% of the active material.
• a physician will determine the actual dosage which will be most suitable for an individual subject.
• the specific dose level and frequency of dosage for any particular individual may be varied and will depend upon a variety of factors including the activity of the specific compound employed, the metabolic stability and length of action of that compound, the age, body weight, general health, sex, diet, mode and time of administration, rate of excretion, drug combination, the severity of the particular condition, and the individual undergoing therapy.
• the daily dosage level of the agent may be in single or divided doses.
• the daily dose as employed for adult human treatment it will range from 2-100mg/kg body weight, preferably 5-60mg/kg body weight, which may be administered in 1 to 4 daily doses, for example, depending on the route of administration and the condition of the patient.
• each unit will preferably contain 200mg to lg of active ingredient.
• the duration of treatment will be dictated by the rate of response rather than by arbitrary numbers of days.
• the group X a R ⁇ ia is R.H as defined for formula (I) or a group convertible to XR 11 .
• aromatic urethane type protecting groups e.g. benzyloxycarbonyl (Cbz) and substituted Cbz, and 9-fluorenylmethoxycarbonyl (Fmoc)
• aliphatic urethane protecting groups e.g. t-butyloxycarbonyl (Boc)
• alkyl type protecting groups e.g. benzyl, trityl and chlorotrityl.
• oxygen protecting groups may include for example alkyl silyl groups, such as trimethylsilyl or tert-butyldimethylsilyl; alkyl ethers such as tetrahydropyranyl or tert-butyl; or esters such as acetate.
• Hydroxy groups may be protected by reaction of for example acetic anhydride, benzoic anhydride or a trialkylsilyl chloride in an aprotic solvent.
• aprotic solvents are dichloromethane, N,N-dimethylformamide, dimethylsulfoxide, tetrahydrofuran and the like.
• Suitable activated derivatives of the carboxyl group include the corresponding acyl halide, mixed anhydride or activated ester such as a thioester.
• the reaction is preferably carried out in a suitable aprotic solvent such as a halohydrocarbon (e.g. dichloromethane) or N,N- dimethylformamide optionally in the presence of a tertiary organic base such as dimethylaminopyridine or triethylamine or in the presence of inorganic base (eg sodium hydroxide) and at a temperature within the range of 0° to 120°C.
• a aprotic solvent such as a halohydrocarbon (e.g. dichloromethane) or N,N- dimethylformamide
• a tertiary organic base such as dimethylaminopyridine or triethylamine
• inorganic base eg sodium hydroxide
• the compounds of formula (II) and (III) may also be reacted
• compounds of formula (I) wherein d is an integer from 1 to 5 and U is -N(R30)- may be prepared by reaction of compounds of formula (V),
• d is an integer from 1 to 5 and L is a suitable leaving group, with X a Rl ia (TV) in which U is -N(R30)-.
• a solvent such as a halohydrocarbon (e.g. dichloromethane), an ether (e.g.
• bases examples include organic bases such as diisopropylethylamine, triethylamine and l,8-diazabicyclo[5.4.0]undec-7-ene, and inorganic bases such as potassium hydroxide, cesium hydroxide, tetraalkylammonium hydroxide, sodium hydride, potassium hydride and the like.
• Suitable leaving groups for this reaction include halides (e.g. chloride, bromide or iodide) and sulfonyloxy groups (e.g. tosyloxy or methanesulfonyloxy).
• Compounds of formula (V) may be prepared by reaction of a compound of formula (11), wherein R 2 is a hydroxyl protecting group, with a suitable activated derivative of the carboxylic acid HOC(O)(CH 2 ) ⁇ L (VI), wherein L is a suitable leaving group as above defined.
• Suitable activated derivatives of the carboxyl group are those defined above for carboxylic acid (III). The reaction is carried out using the conditions described above for the reaction of a compound of formula (II) with carboxylic acid (III).
• -N(R30)- may be prepared by Michael reaction of a compound of formula (VII) wherein R 2 is optionally a hydroxy protecting group
• reaction is suitably carried out in a solvent such as dimethylsulfoxide, N,N-dimethylformamide, 1-methyl-pyrrolidone, a halohydrocarbon (e.g. dichloromethane), an ether (e.g. tetrahydrofuran or dimethoxyethane), acetonitrile or alcohol (e.g methanol or isopropanol) and the like, and in the presence of a base, followed, if desired, by removal of hydroxyl protecting group R 2 and conversion of the
• -CH 2 SCH 2 - or -(CH 2 ) a NR 7 - are known compounds or they may be prepared by analogous methods to those known in the art. Thus, they can be prepared according to the procedures described in US 2002/0025959 Al.
• R34 carboxyl protecting group include t-butyl, allyl or benzyl.
• Compounds of formula (III) may also be prepared by reaction of X a R ⁇ 1 & (V) with acrylonitrile followed by hydrolysis of the nitrile to the acid.
• L is a suitable leaving group such as chlorine, fluorine or bromine
• Compounds of formula (I) may be converted into other compounds of formula (I).
• compounds of formula (I) wherein B is -S(O) z - and z is 1 or 2 may be prepared by oxidation of the corresponding compound of formula (I) wherein z is 0.
• the oxidation is preferably carried out using a peracid, e.g. peroxybenzoic acid, followed by treatment with a phosphine, such as triphenylphosphine.
• the reaction is suitably carried out in an organic solvent such as methylene chloride.
• Compounds of formula ( ⁇ ) wherein U or B is -N(R30)- and R 3 ⁇ is C ⁇ _ 4alkyl can be prepared from compounds wherein R 0 is hydrogen by reductive alkylation.
• R3 is Ci _4alkyl, or C3_6alkenyl optionally substituted by 9 to 10 membered fused bicyclic heteroaryl and R ⁇ is hydrogen are known compounds or they may be prepared by analogous methods to those known in the art. Thus they can be prepared according to the procedures described in US 6262030.
• R 4 or R 5 are hydroxy or R 4 and R 5 taken together with the mtervening atoms form a cyclic group having the following structure:
• Y is a bivalent radical selected from -O- and -N(R13)_ 5 and R is C ⁇ _4alkyl, or C3_ galkenyl optionally substituted by 9 to 10 membered fused bicyclic heteroaryl are known compounds or they may be prepared by analogous methods to those known in the art. Thus they can be prepared according to the procedures described in EP 307177, EP 248279, WO 0078773, WO 9742204.
• R6 is hydrogen and R is Cj .4 alkyl may be prepared by decarboxylation of a compound of formula (XI), wherein R 3 ⁇ is hydroxy protecting group followed, if required, by removal of the protecting group R 2 or R35.
• the decarboxylation may be carried out in the presence of a lithium salt such as lithium chloride, preferably in an organic solvent such as dimethylsulfoxide.
• a lithium salt such as lithium chloride
• organic solvent such as dimethylsulfoxide.
• R3 is C ⁇ _4 alkyl may be prepared according to the procedures described in WO 02/50091 and WO 02/50092.
• DBU for l,8-diazabicyclo[5.4.0]undec-7- ene
• DCM for dichloromethane
• DMAP for 4-dimethylaminopyridine
• DMF for N,N- dimethylformamide
• DMSO for dimethyl sulfoxide
• EDAC.HCl for l-(3- dimethylan ⁇ mopropyl)-3-ethylcarbodiimide hydrochloride
• EtOAc for ethyl acetate
• KO-t-Bu for potassium tert-butoxide
• MeOH for methanol
• TEA for triethylamine and THF for tetrahydrofuran
• MTBK for methyl iso-buthyl ketone.
• 2'-O-Acetyl-6-O methyl-erythromycin A may be prepared by the procedure described by W. R. Baker et al. in J. Org. Chem. 1988, 53, 2340, 2'-0-acetyl-azitbromycin and 2'-0-acetyl- azithromycin- 11,12-carbonate may be prepared by the procedures described by S. Djokic et al. in J. Chem. Res. (S) 1988, 152 and 11 -O-methyl-azithromycin may be prepared by the procedure described by G.Kobrehel et al. in J. Antibiotics 1992, 45,521-532.
• 9(E)- Ethoxyimmo-erythromycin A may be prepared by the procedures described in EP 1 167 375.
• 6-O-Ethyl erythromycin A, 6-0-propyl erythromycin A and 9-(l-isopropoxy- cyclohexyl)oxirnino-erythromycin A may be prepared by procedure described in US Patent 4,990,602 and Bioorg.Med.Chem.Lett. 2000, 10, 815-819.
• 6-O-Methyl-8a-aza-8a- homoerythromycin A may be prepared by procedure described in US patent_6, 110,965.
• 6,9-cyclic ether may be prepared by procedure described in Experientia 1971, 27, 362.
• 2'-C>-Acetyl-C>-(9J?)-acety ⁇ hydroximino erythromycin A may be prepared by procedure described in WO2004/039822.
• 4"-0-Propenoyl-azithromycin may be prepared by procedure described in WO03/042228.
• l-Cyclopropyl-6-iodo-4- oxo-l,4-dihydro-qunoline-3-carboxylic acid ethyl ester, 7-chloro-l-isopropyl-6-fluoro-4-oxo- l,4-dihydro-qurnoline-3 -carboxylic acid and 7-chloro-l-tert-butyl-6-fluoro-4-oxo-l,4- dihydro-quinoline-3-carboxylic acid may be prepared by the procedure described in J.Med.Chem., 1995, 38, 973.
• l-Ethyl-6-iodo-4-oxo-l,4-dihydro-qunoline-3-carboxylic acid ethyl ester my be prepared by procedure described hiAust. J. Chem., 1973, 26, 907.
• Ethyl 1- emyl-6-hydroxy-4-oxo-l,4-dmydro-3-qumolinecarboxylate may be prepared by the procedure described in GB 1433774.
• Trifluoroacetic acid (0.386 mL) was added into solution of Intermediate 9c (0.42 g) in MeCN (5 mL) at room temperature. The solution has been stirring at room temperature for 48 hours and evaporated yielding 0.80 g of the title compound.
• a Pyrex tube was charged with sodium tert-butoxide (1.4 mmol), Pd 2 (dba) 3 (0.00125 mmol), and BL AP (0.00375 mmol).
• the Pyrex tube was fitted with a septum and after the air atmosphere was replaced with argon, toluene (4 mL), l-ethyl-6-iodo-4-oxo-l,4-dihydro- qunoline-3 -carboxylic acid ethyl ester (1.0 mmol), and benzophenone imine (1.2 mmol) were added by syringe.
• the reaction was sealed and heated to 80°C with stirring until starting material was consumed as judged by GC analysis.
• the reaction mixture was cooled to room temperature, diluted with ether (40 mL), filtered, and concentrated.
• the crude reaction mixture was then recrystallized from MeOH to furnish the desired product in 90% yield.
• Example 1 Hydrogenation of Example 1 (0.25 mg) in methanol (20 mL) with addition of 10 % Pd/C (100 mg) at 5 bar for 20 hours yielded the title product.
• MS (ES+) m/z : [MH] + 1174.8
• Example 9 4"-O-(3-(2-f2-(3-carboxy-7-chloro-l-cvclopropyl-4-oxo-l,4-dihydro-quinolin-6- ylaminoVethoxyl -ethoxyl-pr opionyl " )-! 1-O-methyl-azithromycin Using the procedure of Example 6, Intermediate? and 2'-O-acetyl-ll-0-methyl- azithromycin gave the title compound. Product was purified by column chromatography
• Example 9 To a solution of Example 9 (0.3g, 0.25 mmol) in EtOAc (2.5 mL) acetic acid (0.032mL, 0.56 mmol) was added under stirring in an ice bath. Addition of diisopropylether (15mL) and n-hexane (30 mL) yielded 0,289g of precipitated acetate salt. (92,5% pure).
• Example 8 (300 mg, 0.26 mmol) was dissolved in 20 mL of ethanol, 100 mg of 10 % Pd/C was added and the mixture was stired under H 2 pressure (5 bar) for 15 hours.
• Example 12 4"-0-t2- ⁇ 3-f2-(3-Carboxy-7-chIoro-l-cyclopropyl-4-oxo-l,4-dihydro-quinolin-6-yIoxy)- ethoxyl-propionylaminol-acetvIVazithromvcin 11.12-cyclic carbonate
• Example 14 4"-0-(2-f3-f2-(3-Carboxy-6-fluoro-l-cvclopropyl-8-methoxy-4-oxo-l,4-dihydro- quinoIin-7-ylamino)-ethoxyl-propionylamino ⁇ -acetyl)-azithromycin 11,12-cyclic carbonate
• Example 17 4"-Q-f2-r3-((2-r3-(6-Ethoxycarbonyl-7-oxo-2.3-dihvdro-lH.7H-pyridof3,2J-iilquinoMn- 9-vIVprop-2-vnvIaminol -ethyll-propyI-aminoVpropionylaminol-acetyll-azithromvcin
• Example 19
• Example 20 4 -Q-r3- - ⁇ 2-r2-f3-Carboxy-7-chIoro-l-cvcIopropyl-4-oxo-l,4-dihvdro-quinoIin-6- ylamino -ethoxyl-ethoxy
• Example 25 4' , -0-f3- ⁇ 2-f2-(3-Carboxy-6-fluoro-l-cvclopropyl-4-oxo-l,4-dihvdro-quinolin-7- ylaminoVethoxyI-ethoxyl-propionylVazithromvcin
• Example 26 4"-0-(3-(2-f2-(3-Carboxy-7-chloro-l-cvclopropyI-4-oxo-l,4-dihvdro-quinolin-6- ylaminoVethoxyl-ethoxyl-propionyl)-6-0-methyl-ervthromvcin A 11.12-cvclic carbamate
• Example 8 (200 mg, 0.17 mmol) was dissolved in DCM (5 mL), and to that solution NaHCO 3 (67 mg, 0.79 mmol) and propionic acid anhydride (0.27 ml, 0.207 mmol) were added and stirred at R.T. overnight.
• Example 29A (100 mg, 0.086 mmol) yielded 90 mg of the title compound.
• HPLC/MS (ES) m/z: [MH] + 1124.5
• C-NMR 500MHz, DMSO-d6): 8.90, 10.40, 11.85, 15.89, 16.93, 17.62, 18.05, 19.77, 20.26, 20.63, 20.77, 21.52, 26.21, 30.14, 34.25, 34.90, 37.93, 40.26, 42.47, 43.62, 44.25, 48.86, 50.10, 52.56, 62.40, 64.51, 66.07, 66.82, 68.66, 68.85, 69.58, 70.60, 72.07, 74.10, 75.91, 77.03, 77.75, 77.83, 79.20, 95.44, 100.29, 101.88, 120.34, 127.11, 128.48, 129.81, 143.90, 146.71, 166.91, 170.87, 175.02, 176.40, 218.63.
• Example 36 (50 mg) gave the title compound.
• Product was precipitated from EtOAc: n-hexane yielding 80% pure title compound (24 mg).
• MS (ES+) m z [MH] + 1121.2.
• Example 43 To the solution of Example 43 (166 mg) in CH 2 C1 2 (1 mL) at R.T. acetic acid (16 ⁇ l, 2 eq) was added and the product was precipitated by addition of di-z'-propyl ether (10 mL) yielding 148 mg of the title product.
• Example 47 4"-0-(3- ⁇ 2-[2-(3-Carboxy-l-cyclopropyl-7-methoxy-4-oxo-l,4-dihvdro-quinolin-6- ylammoVethoxyl-ethoxyl-propionvD-azithromvcin
• Example 50 4"-0-(3-f2-[3-f3-carboxy-l-ethyI-4-oxo-1.4-dihvdro-quinolin-6-yl)-propoxyl-ethoxy)- propionvD-azithromycin diacetate salt
• EtOAc 10 mL
• acetic acid 0.017 mL, 0,29 mmol
• Precipitate occurred after addition of n- hexane (100 mL), it was filtered off yielding the titled product (135 mg).
• Example 51 4"-0-(3-f2-[3-f3-carboxy-l-ethyI-4-oxo-1.4-dihvdro-quinolin-6-yl)-propoxyl-ethoxy)- propionvD-azithromycin diacetate salt
• Example 61 4"-O-(3- ⁇ 2-f2-(3-Carboxy-7-chloro-l-cvclopropyl-4-oxo-l,4-dihvdro-quinolin-6- ylaminoV ethoxy] -ethylaminol-propionvD-azithromvcin (A) 4 ⁇ -O-(3- ⁇ 2-f2-f3-Carboxy-6-fluoro-l-cvclopropyl-4-oxo-l,4-dihvdro-quinolin-7- ylaminoVethoxyl-ethvIaminol-propionvO-azithromvcm fB)
• Example 55 (80 mg, 0.068 mmol) was dissolved in 30 mL of methanol, 40 mg of 10 % Pd/C was added and the mixture was stired under H 2 pressure (5 bar) for 6 hours. The reaction mixture was filtrated and solvent evaporated in vacuum. Crude product was diluted in isopropylacetate
• Example 75 A solution of Example 75 (0.032 g, 0.049 mmol) dissolved in a mixture of chloroform (0.7 mL), 37% aqueous formaldehyde (7 uL) and formic acid (7 uL) was stirred at 60°C. After 4 h the mixture was cooled, evaporated and the residue subjected to chromatography over silica gel eluting with with 0 - 10% [9:1 methanol/20 M ammonia] in dichloromethane gave the title compound in 58 % yield.
• ESMS m/z [MH] + 1137.0.

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