WO2005068612A3 - Cellules progenitrices hepatiques humaines et procedes d'utilisation correspondants - Google Patents

Cellules progenitrices hepatiques humaines et procedes d'utilisation correspondants Download PDF

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Publication number
WO2005068612A3
WO2005068612A3 PCT/IB2005/000660 IB2005000660W WO2005068612A3 WO 2005068612 A3 WO2005068612 A3 WO 2005068612A3 IB 2005000660 W IB2005000660 W IB 2005000660W WO 2005068612 A3 WO2005068612 A3 WO 2005068612A3
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WO
WIPO (PCT)
Prior art keywords
progenitor cells
methods
human hepatic
hepatic progenitor
well
Prior art date
Application number
PCT/IB2005/000660
Other languages
English (en)
Other versions
WO2005068612A2 (fr
Inventor
Suchitra Holgersson
Original Assignee
Novahep Ab
Suchitra Holgersson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novahep Ab, Suchitra Holgersson filed Critical Novahep Ab
Priority to EP05708747A priority Critical patent/EP1711597B1/fr
Priority to AT05708747T priority patent/ATE548447T1/de
Priority to ES05708747T priority patent/ES2385248T3/es
Priority to AU2005205240A priority patent/AU2005205240B2/en
Priority to CA2553303A priority patent/CA2553303C/fr
Priority to JP2006548478A priority patent/JP5588587B2/ja
Publication of WO2005068612A2 publication Critical patent/WO2005068612A2/fr
Publication of WO2005068612A3 publication Critical patent/WO2005068612A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5067—Liver cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G01N33/5017—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity for testing neoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/12—Hepatocyte growth factor [HGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/165—Vascular endothelial growth factor [VEGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/02—Hepadnaviridae, e.g. hepatitis B virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/085—Picornaviridae, e.g. coxsackie virus, echovirus, enterovirus
    • G01N2333/10—Hepatitis A virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)

Abstract

L'invention concerne des cellules progénitrices immunoréactives du foie à CD117, ainsi qu'à CD34 capables de proliférer dans une culture et de se différentier in vivo en cellules hépatocytes, cholangiocytes ou sinusoïdales. Les cultures peuvent être étalées sur un nombre élevé de passages et bien s'intégrer après la transplantation dans un foie adulte.
PCT/IB2005/000660 2004-01-14 2005-01-14 Cellules progenitrices hepatiques humaines et procedes d'utilisation correspondants WO2005068612A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP05708747A EP1711597B1 (fr) 2004-01-14 2005-01-14 Cellules progenitrices hepatiques humaines et procedes d'utilisation correspondants
AT05708747T ATE548447T1 (de) 2004-01-14 2005-01-14 Menschlicher hepatische vorläuferzellen und verfahren zur verwendung davon
ES05708747T ES2385248T3 (es) 2004-01-14 2005-01-14 Células progenitoras hepáticas humanas y métodos de uso de las mismas
AU2005205240A AU2005205240B2 (en) 2004-01-14 2005-01-14 Human hepatic progenitor cells and methods of use thereof
CA2553303A CA2553303C (fr) 2004-01-14 2005-01-14 Cellules progenitrices hepatiques humaines et procedes d'utilisation correspondants
JP2006548478A JP5588587B2 (ja) 2004-01-14 2005-01-14 ヒトの肝臓前駆細胞およびその使用方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US53640504P 2004-01-14 2004-01-14
US60/536,405 2004-01-14
US62300304P 2004-10-27 2004-10-27
US60/623,003 2004-10-27

Publications (2)

Publication Number Publication Date
WO2005068612A2 WO2005068612A2 (fr) 2005-07-28
WO2005068612A3 true WO2005068612A3 (fr) 2006-03-30

Family

ID=34798866

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2005/000660 WO2005068612A2 (fr) 2004-01-14 2005-01-14 Cellules progenitrices hepatiques humaines et procedes d'utilisation correspondants

Country Status (8)

Country Link
US (1) US20060040386A1 (fr)
EP (1) EP1711597B1 (fr)
JP (2) JP5588587B2 (fr)
AT (1) ATE548447T1 (fr)
AU (1) AU2005205240B2 (fr)
CA (1) CA2553303C (fr)
ES (1) ES2385248T3 (fr)
WO (1) WO2005068612A2 (fr)

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US7780993B2 (en) * 2001-09-07 2010-08-24 Yeda Research And Development Co. Ltd. Therapeutic transplantation using developing, human or porcine, renal or hepatic, grafts
IL165425A0 (en) * 2004-11-28 2006-01-15 Yeda Res & Dev Methods of treating disease by transplantation of developing allogeneic or xenogeneic organs or tissues
EP1915461B1 (fr) 2005-08-17 2018-08-01 Dx4U GmbH Composition et methode pour la determination de l'expression de ck-19
GR1005424B (el) * 2005-08-17 2007-02-01 Medexis Αεβε Μεθοδος για τον ποσοτικο προσδιορισμο της εκφρασης της ck19
US8124749B2 (en) 2007-06-12 2012-02-28 Case Western Reserve University Targeted cell death
CA2694696A1 (fr) * 2007-07-27 2009-02-05 Immuneregen Biosciences, Inc. Procedes et compositions pour stimuler la proliferation ou la differenciation de cellules souches par la substance p ou un analogue de celle-ci
EP2295537A4 (fr) * 2008-05-14 2011-11-30 Public Univ Corp Yokohama City Cellule souche hépatique humaine, son procédé de préparation, procédé d induction de sa différenciation et son procédé d utilisation
US20110136101A1 (en) * 2009-12-04 2011-06-09 Jan Holgersson Immortalized Human Fetal Liver Cells
EP2333047A1 (fr) * 2009-12-09 2011-06-15 Fresenius Medical Care Deutschland GmbH Support conditionné dérivé de cellule souche adulte et/ou cellules souches adultes à utiliser dans le traitement thérapeutique d'une maladie tumorale
US9533013B2 (en) * 2013-03-13 2017-01-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree
US11066649B2 (en) * 2014-03-19 2021-07-20 Institut National De La Sante Et De La Recherche Medicale (Inserm) Method for inducing human cholangiocyte differentiation
JP2023516484A (ja) 2020-03-11 2023-04-19 ビット バイオ リミテッド 肝細胞作製方法
TW202321440A (zh) * 2021-10-07 2023-06-01 美商肝特斯公司 追蹤接受者中之供體細胞之方法

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JP2004510432A (ja) * 2000-10-03 2004-04-08 ユニバーシティ　オブ　ノース　カロライナ 両能性肝前駆細胞の単離方法
CA2424779C (fr) * 2000-10-03 2016-08-23 University Of North Carolina At Chapel Hill Procede de multiplication par clonage de cellules souches hepathiques
DE60229242D1 (de) * 2001-05-16 2008-11-20 Kanagawa Kagaku Gijutsu Akad Verfahren zum nachweis und zur trennung undifferenzierter leberzellen mit dlk
US20030138951A1 (en) * 2001-10-18 2003-07-24 Li Yin Conversion of liver stem and progenitor cells to pancreatic functional cells
AU2003251397B2 (en) * 2002-06-05 2009-10-01 Genentech, Inc. Compositions and methods for liver growth and liver protection
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Title
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CROSBY H A ET AL: "Human hepatic stem-like cells isolated using c-kit or CD34 can differentiate into biliary epithelium.", GASTROENTEROLOGY, vol. 120, no. 2, February 2001 (2001-02-01), pages 534 - 544, XP002358744, ISSN: 0016-5085 *
GERLACH J C ET AL: "Use of primary human liver cells originating from discarded grafts in a bioreactor for liver support therapy and the prospects of culturing adult liver stem cells in bioreactors: A morphologic study.", TRANSPLANTATION, vol. 76, no. 5, 15 September 2003 (2003-09-15), pages 781 - 786, XP002358743, ISSN: 0041-1337 *
GUETTIER C: "Quelle(s) cellule(s) souche(s) pour la régénération du foie adulte ?", ANNALES DE PATHOLOGIE, vol. 25, no. 1, February 2005 (2005-02-01), pages 33 - 44, XP008057007, ISSN: 0242-6498 *
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Also Published As

Publication number Publication date
ES2385248T3 (es) 2012-07-20
JP2011103884A (ja) 2011-06-02
EP1711597B1 (fr) 2012-03-07
ATE548447T1 (de) 2012-03-15
AU2005205240B2 (en) 2011-04-21
CA2553303A1 (fr) 2005-07-28
CA2553303C (fr) 2014-06-10
JP2007523638A (ja) 2007-08-23
EP1711597A2 (fr) 2006-10-18
WO2005068612A2 (fr) 2005-07-28
AU2005205240A1 (en) 2005-07-28
JP5588587B2 (ja) 2014-09-10
US20060040386A1 (en) 2006-02-23

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