WO2005051970A3 - Hochparalleler dna-synthesizer auf matrizenbasis - Google Patents

Hochparalleler dna-synthesizer auf matrizenbasis Download PDF

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Publication number
WO2005051970A3
WO2005051970A3 PCT/EP2004/013131 EP2004013131W WO2005051970A3 WO 2005051970 A3 WO2005051970 A3 WO 2005051970A3 EP 2004013131 W EP2004013131 W EP 2004013131W WO 2005051970 A3 WO2005051970 A3 WO 2005051970A3
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WO
WIPO (PCT)
Prior art keywords
matrices
nucleic acid
highly parallel
parallel dna
dna synthesiser
Prior art date
Application number
PCT/EP2004/013131
Other languages
English (en)
French (fr)
Other versions
WO2005051970A2 (de
Inventor
Peer Staehler
Cord Staehler
Markus Beier
Original Assignee
Febit Ag
Peer Staehler
Cord Staehler
Markus Beier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Febit Ag, Peer Staehler, Cord Staehler, Markus Beier filed Critical Febit Ag
Priority to US10/579,769 priority Critical patent/US20070087349A1/en
Priority to EP04803181.9A priority patent/EP1685261B1/de
Publication of WO2005051970A2 publication Critical patent/WO2005051970A2/de
Publication of WO2005051970A3 publication Critical patent/WO2005051970A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Herstellung von Nukleinsäuredoppelsträngen auf Matrizenbasis. Die Matrizen für die enzymbasierte Synthese mittels Kopiervorgang bestehen ihrerseits aus kopierfähigenNukleinsäurepolymeren, die in Form einer Array-Anordnung auf einem gemeinsamen Träger synthetisiert werden. Sie stehen nach ihrer eigentlichen Synthese in unter Zugabe entsprechender Reagenzien und Hilfsstoffe, wie Nukleotiden, vervielfältigt werden.
PCT/EP2004/013131 2003-11-18 2004-11-18 Hochparalleler dna-synthesizer auf matrizenbasis WO2005051970A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/579,769 US20070087349A1 (en) 2003-11-18 2004-11-18 Highly parallel template-based dna synthesizer
EP04803181.9A EP1685261B1 (de) 2003-11-18 2004-11-18 Hochparalleler dna-synthesizer auf matrizenbasis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10353887.9 2003-11-18
DE10353887A DE10353887A1 (de) 2003-11-18 2003-11-18 Hochparalleler DNA-Synthesizer auf Matrizenbasis

Publications (2)

Publication Number Publication Date
WO2005051970A2 WO2005051970A2 (de) 2005-06-09
WO2005051970A3 true WO2005051970A3 (de) 2005-07-28

Family

ID=34585201

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/013131 WO2005051970A2 (de) 2003-11-18 2004-11-18 Hochparalleler dna-synthesizer auf matrizenbasis

Country Status (4)

Country Link
US (1) US20070087349A1 (de)
EP (1) EP1685261B1 (de)
DE (1) DE10353887A1 (de)
WO (1) WO2005051970A2 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006039479A1 (de) 2006-08-23 2008-03-06 Febit Biotech Gmbh Programmierbare Oligonukleotidsynthese
DE102007018833A1 (de) 2007-04-20 2008-10-23 Febit Holding Gmbh Verbesserte molekularbiologische Prozessanlage
DE102006062089A1 (de) 2006-12-29 2008-07-03 Febit Holding Gmbh Verbesserte molekularbiologische Prozessanlage
EP2053132A1 (de) * 2007-10-23 2009-04-29 Roche Diagnostics GmbH Anreicherung und Sequenzanalyse von Genombereichen
DE102007056398A1 (de) 2007-11-23 2009-05-28 Febit Holding Gmbh Flexibles Extraktionsverfahren für die Herstellung sequenzspezifischer Molekülbibliotheken
EP3257942B1 (de) 2008-12-18 2020-07-22 ITI Scotland Limited Verfahren zum zusammensetzen von polynukleinsäuresequenzen
WO2010094772A1 (en) 2009-02-20 2010-08-26 Febit Holding Gmbh Synthesis of sequence-verified nucleic acids
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
KR102291045B1 (ko) 2013-08-05 2021-08-19 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10844373B2 (en) 2015-09-18 2020-11-24 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
GB2568444A (en) 2016-08-22 2019-05-15 Twist Bioscience Corp De novo synthesized nucleic acid libraries
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
CA3047128A1 (en) 2016-12-16 2018-06-21 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CA3054303A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
JP2020536504A (ja) 2017-09-11 2020-12-17 ツイスト バイオサイエンス コーポレーション Ｇｐｃｒ結合タンパク質およびその合成
WO2019079769A1 (en) 2017-10-20 2019-04-25 Twist Bioscience Corporation HEATED NANOWELLS FOR THE SYNTHESIS OF POLYNUCLEOTIDES
AU2019205269A1 (en) 2018-01-04 2020-07-30 Twist Bioscience Corporation DNA-based digital information storage
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
SG11202109283UA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for antibody optimization
CN113766930A (zh) 2019-02-26 2021-12-07 特韦斯特生物科学公司 Glp1受体的变异核酸文库
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
WO2023035003A1 (en) 2021-09-03 2023-03-09 Elegen Corp. Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
WO1999005321A1 (en) * 1997-07-22 1999-02-04 Rapigene, Inc. Amplification and other enzymatic reactions performed on nucleic acid arrays
WO1999032654A1 (en) * 1997-12-22 1999-07-01 Hitachi Chemical Co., Ltd. Direct rt-pcr on oligonucleotide-immobilized pcr microplates
WO2000049142A1 (de) * 1999-02-19 2000-08-24 Febit Ferrarius Biotechnology Gmbh Verfahren zur herstellung von polymeren
WO2001048242A2 (en) * 1999-12-29 2001-07-05 Mergen Ltd. Methods for amplifying and detecting multiple polynucleotides on a solid phase support

Family Cites Families (3)

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US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
EP1598432A3 (de) * 2000-07-31 2006-06-07 Agilent Technologies, Inc. Array-basierende Methoden zur Synthese von Nukleinsäuregemischen
US20040203085A1 (en) * 2001-04-26 2004-10-14 Andre Bernard Method for parallel synthesis and transfer of molecules to a substrates

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
WO1999005321A1 (en) * 1997-07-22 1999-02-04 Rapigene, Inc. Amplification and other enzymatic reactions performed on nucleic acid arrays
WO1999032654A1 (en) * 1997-12-22 1999-07-01 Hitachi Chemical Co., Ltd. Direct rt-pcr on oligonucleotide-immobilized pcr microplates
WO2000049142A1 (de) * 1999-02-19 2000-08-24 Febit Ferrarius Biotechnology Gmbh Verfahren zur herstellung von polymeren
WO2001048242A2 (en) * 1999-12-29 2001-07-05 Mergen Ltd. Methods for amplifying and detecting multiple polynucleotides on a solid phase support

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BEIER M ET AL: "Analysis of DNA-microarrays produced by inverse in situ oligonucleotide synthesis", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS B.V., AMSTERDAM, NL, vol. 94, no. 1, 14 March 2002 (2002-03-14), pages 15 - 22, XP002281008, ISSN: 0168-1656 *
WANG J ET AL: "DNA microarrays with unimolecular hairpin double-stranded DNA probes: Fabrication and exploration of sequence-specific DNA/protein interactions", JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, AMSTERDAM, NL, vol. 55, no. 3, March 2003 (2003-03-01), pages 215 - 232, XP002251375, ISSN: 0165-022X *

Also Published As

Publication number Publication date
WO2005051970A2 (de) 2005-06-09
DE10353887A1 (de) 2005-06-16
US20070087349A1 (en) 2007-04-19
EP1685261B1 (de) 2015-03-25
EP1685261A2 (de) 2006-08-02

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