WO2005035745A1 - ウイルス不活性化方法およびウイルス不活性化装置 - Google Patents
ウイルス不活性化方法およびウイルス不活性化装置 Download PDFInfo
- Publication number
- WO2005035745A1 WO2005035745A1 PCT/JP2004/015074 JP2004015074W WO2005035745A1 WO 2005035745 A1 WO2005035745 A1 WO 2005035745A1 JP 2004015074 W JP2004015074 W JP 2004015074W WO 2005035745 A1 WO2005035745 A1 WO 2005035745A1
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- Prior art keywords
- processing
- gas
- virus
- processing gas
- bag
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/20—Combustible or heat-generating compositions
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2796/00—Viruses not covered by groups C12N2710/00 - C12N2795/00
Definitions
- the present invention relates to a method and an apparatus for inactivating viruses.
- Patent Document 2 Patent Document 3
- Patent Document 5 Patent Document 5
- Patent Document 1 JP-A-2002-282346
- Patent Document 2 Japanese Patent Application Laid-Open No. 2002-85898
- Patent Document 3 Japanese Utility Model Registration No. 3047747
- Patent Document 4 JP 2001-182346 A
- Patent Document 5 JP-A-2000-106856
- the problem to be solved by the present invention is to solve the above-mentioned problems of the conventional technology, that is, a new virus virus that can inactivate a virus existing in a processing object without using ozone or ultraviolet rays.
- An object of the present invention is to provide an activation method and apparatus. Means for solving the problem
- a process gas containing an active species derived from at least one of methanol, ethanol, and formaldehyde is generated while the process gas is generated.
- the active species derived from at least one of methanol, ethanol, and formaldehyde have the strength, nucleic acid destruction ability and strength, and osmotic force.
- virus inactivation processing does not corrode metal parts or weaken plastic parts in the space to be processed.
- the colors and patterns of clothing and bedding in the space to be processed will not fade, and the texture and touch will not be impaired! /.
- the virus inactivating apparatus of the present invention generates a processing gas containing an active species derived from at least one of methanol, ethanol, and formaldehyde, and a processing container capable of containing and sealing a processing target.
- the processing gas generator and the gas transport system are operated under the control. I do.
- the processing gas generator generates a processing gas containing active species derived from at least one of methanol, ethanol, and formaldehyde.
- the gas transfer system introduces the processing gas generated by the processing gas generator into the processing container.
- the virus present inside or on the surface of the processing object stored in the processing container is inactivated by the action of the processing gas. Since the processing gas used in this apparatus has a strong nucleic acid destruction ability and a strong penetrating power, viruses existing inside or on the surface of the processing object can be efficiently inactivated. This virus inactivation processing does not corrode the metal parts constituting the processing target or weaken the plastic parts. Even when clothing and bedding are to be processed, their colors and patterns do not fade, and their texture and texture are not impaired.
- the processing gas is a methanol gas.
- a gas containing an active species generated by a decomposition reaction or a gas containing an active species generated by heating an aqueous formaldehyde solution or paraformaldehyde is preferable.
- viruses existing in the processing target can be efficiently inactivated.
- FIG. 1 is a perspective view showing an embodiment of a virus inactivating apparatus for carrying out the method of the present invention.
- FIG. 2 is a perspective view of a processing bag in FIG. 1.
- FIG. 3 is a configuration diagram of a processing gas supply device in FIG. 1.
- FIG. 4 is a configuration diagram of a processing gas generator in FIG. 3.
- FIG. 5 is a view showing experimental results of virus inactivation treatment for swine parpovirus (PPV).
- FIG. 6 is a view showing an experimental result of virus inactivation treatment for hepatitis A virus (HAV).
- FIG. 1 is a perspective view showing an embodiment of the virus inactivating device of the present invention.
- the virus inactivating device 10 includes a processing bag (processing container) 20 for storing an object to be processed such as clothing and bedding, and a processing gas supply device 30 for supplying a processing gas into the sealed processing bag 20. You.
- FIG. 2 is a perspective view of the processing bag 20.
- the processing bag 20 is formed of a transparent film having resistance to reagents, heat resistance, and pressure resistance, and has an opening 21 at one end for inserting and removing an object to be processed.
- the opening 21 is provided with a lid member 22 having a heat seal 23 along the edge.
- the opening 21 is closed by the lid member 22, and the heat seal 23 is heated and brought into close contact with the main body of the processing bag 20, whereby the processing bag 20 can be completely sealed.
- an inlet 24 for introducing the processing gas and an outlet 25 for discharging the exhaust gas are provided on the side of the processing bag 20.
- the inlet 24 and the outlet 25 are normally closed, and are opened only when the distal end of the processing gas inlet pipe 31 and the distal end of the exhaust pipe 32 of the processing gas supply device 30 are connected.
- FIG. 3 is a configuration diagram of the processing gas supply device 30.
- the processing gas supply device 30 includes a processing gas generator 40, a gas transport system 50 for introducing the processing gas generated by the processing gas generator 40 into the sealed processing bag 20, and a processing gas generator. 40 and a controller 60 for controlling the operation of the gas transport system 50.
- FIG. 4 is a configuration diagram of the processing gas generator 40.
- a methanol supply source (not shown) is supplied with methanol through a transfer pipe 41, a first temperature controller 43 for heating the vaporization chamber 42 from the surroundings, A substantially cylindrical catalyst tank 44 connected to the fuel cell and a second temperature controller 45 for heating the catalyst tank 44 from the surroundings are provided.
- the catalyst tank 44 is filled with a granulated catalyst 46.
- platinum, copper, aluminum, carbon, or a mixture thereof is used.
- the catalyst acts on the methanol gas to generate a processing gas containing active species generated by the oxidative decomposition reaction of methanol.
- the amount of processing gas generated depends on the amount of methanol vaporized in the vaporization chamber 42, the amount of methanol gas supplied to the catalyst tank 44, the heating temperature of the catalyst tank 44, and the like.
- the gas transfer system 50 includes an intake pump (processing gas transfer device) 51 for sending outside air or a processing gas (a processing gas or a mixed gas of the processing gas and the outside air) into the processing bag 20, and A humidity controller 52 for adjusting the humidity of the supplied processing gas, a temperature controller 53 for adjusting the temperature of the processing gas supplied into the processing bag 20, and an exhaust gas from the processing bag 20 (inactivated). And an exhaust pump (gas ejector) 55 for discharging the treated exhaust gas.
- the exhaust-port-side passage of the exhaust pump 55 and the intake-port-side passage of the intake pump 51 are connected by a return air passage 56.
- the processing gas generator 40, the suction pump 51, the humidity controller 52, the temperature controller 53, the exhaust gas processor 54, and the exhaust pump 55 are controlled by a controller 60.
- the controller 60 controls the humidity controller 52 and the temperature controller 53 while controlling the concentration (generation amount) of the processing gas within a predetermined range by controlling the processing gas generator 40.
- the temperature and humidity of the processing gas supplied into the processing bag 20 are controlled within a predetermined range, and the amount of the processing gas transported into the processing bag 20 and the processing bag are controlled by controlling the suction pump 51 and the exhaust pump 55. Controls the amount of exhaust gas emitted from inside 20.
- virus inactivation processing method by the virus inactivation apparatus 10 configured as described above.
- a specialized worker handles the object to be treated and the treatment bag 20 and operates the treatment gas supply device 30.
- the processing bag 20 At the time of performing the virus inactivation process, bedding, clothing, and the like to be processed are accommodated in the processing bag 20. At this time, a chemical indicator (not shown) is put in with the object to be processed. Thereafter, the opening of the processing bag 20 is closed with the lid member 22 and the heat seal 23 provided on the lid member 22 is heated, whereby the lid member 22 is brought into close contact with the processing bag main body and the processing bag 20 is sealed. . Next, the distal end of the processing gas inlet pipe 31 and the distal end of the exhaust pipe 32 of the processing gas supply device 30 are connected to the inlet 24 and the outlet 25 of the processing bag 20, respectively.
- the processing gas supply device 30 is operated in the virus removal processing mode. Then, the processing gas supply device 30 first exhausts the processing bag 20. That is, the air in the processing bag 20 is discharged by driving the exhaust pump 55 with the intake pump 51 stopped. Next, a processing gas is supplied into the processing bag 20 to perform a virus inactivation process on the processing target housed in the processing bag 20. That is, by controlling the driving of the processing gas generator 40, the suction pump 51, the humidity controller 52, and the temperature controller 53, the temperature and the humidity (hereinafter, relative humidity) are stored within the processing bag 20 respectively. Supply process gas at 40 ° C (preferably 35 ° C) and 80-90% humidity.
- the worker checks the value of the chemical indicator, and if the value is an appropriate value, switches the operation mode of the processing gas supply device 30 to the post-processing mode. Then, the processing gas supply device 30 starts exhausting the processing bag 20. That is, the processing gas generator 40 is stopped, and the suction pump 51 and the exhaust pump 55 are operated to exhaust the inside of the processing bag 20. Then, the exhaust gas from inside the processing bag 20 is processed by the exhaust gas processor 54. The exhaust gas treated by the exhaust gas treatment device 54 is supplied to the intake pump 51 via the recirculating air passage 56 and is supplied again into the treatment bag 20 to circulate the air in the treatment bag 20. This processing is performed until the processing gas concentration in the processing bag 20 becomes lower than a predetermined value.
- the processing object 20 is dried by supplying dry hot air into the processing bag 20. That is, the air in the processing bag 20 is circulated by operating the exhaust pump 55 and the intake pump 51, and the humidity controller 52 keeps the temperature of the air at an appropriate level while the humidity controller 52 dehumidifies the air. Perform
- the worker After completing the above series of steps, the worker removes the processing gas introduction pipe 31 and the exhaust pipe 32 of the processing gas supply device 30 from the inlet 24 and the outlet 25 of the processing bag 20.
- the processing target having been subjected to the virus inactivation processing is stored in the processing bag 20, or taken out of the processing bag 20 and delivered to the processing request source.
- the processing gas containing the active species generated by the oxidative decomposition reaction of methanol is used as the processing gas. This makes it possible to inactivate viruses existing inside or on the surface of the object to be processed at a low processing temperature of 20 ° C. to 40 ° C.
- the concentration, temperature, and humidity of the processing gas introduced into the processing bag 20 are controlled within predetermined ranges, and the amount of the processing gas transported into the processing bag 20 and the amount of exhaust gas discharged are controlled. Therefore, the ability of the processing gas is maximized while the amount of the processing gas used is suppressed, and the virus inactivation treatment can be efficiently performed.
- the processing gas is supplied into the processing bag 20 after the air in the processing bag 20 is exhausted, so that the processing gas of a predetermined concentration can be quickly and rapidly distributed throughout the processing bag 20. Therefore, all viruses existing inside or on the surface of the bedding or clothing to be treated can be inactivated by the treatment gas.
- the virus inactivation apparatus 10 of the above embodiment generates a processing gas containing activated species generated by an oxidative decomposition reaction of methanol by causing a catalyst to act on methanol.
- Power provided with the device 40 Alternatively, a configuration may be adopted in which a processing gas generator that generates a processing gas by applying ultrasonic waves, ultraviolet rays, or plasma to methanol is provided.
- a process for generating a processing gas containing active species by heating an aqueous formaldehyde solution or paraformaldehyde (solid) to a temperature equal to or higher than the vaporization temperature, or by applying ultrasonic waves, ultraviolet rays, plasma, or high-frequency electromagnetic waves thereto. It is good also as composition provided with a gas generator.
- a configuration including a processing gas generator for generating a processing gas containing an active species derived from ethanol may be provided.
- the processing bag 20 is provided as the processing container
- a hard processing container having chemical resistance, heat resistance, and pressure resistance to the processing gas may be used.
- the good thing is, of course.
- the inside of the processing vessel can be depressurized by exhausting the processing vessel before supplying the processing gas. And the virus inactivation treatment of the object to be treated can be performed extremely efficiently.
- the gas transfer system 50 of the processing gas supply device 30 includes only one force pump described in the configuration example including the two pumps 51 and 55, and the processing pump is used by the pump.
- the supply and discharge of gas into and from the container may be configured.
- HAV Hepatitis A virus
- sample preparation 100 ⁇ L of each virus mother liquor was injected into six well plates, dried in a safety cabinet for 2 hours, and sealed with a gas sterilization bag (sample preparation).
- MR gas effectively inactivated viruses regardless of the type of nucleic acid (DNA, RNA). In addition, since the infectivity titer and the LRV results of the quantitative PCR were almost the same, it was indicated that the inactivation of the virus was due to nucleic acid destruction. [0048] 2) MR gas has the same virus inactivation effect regardless of the presence of the pressurization, circulation, and decompression cycles, and therefore has high permeability into the treatment object. It was shown that.
- UV irradiation is said to have a nucleic acid destruction effect! / ⁇ , but suggests that there is no effective virus inactivation and nucleic acid destruction effect.
- HAV Hepatitis A virus
- FIG. 5 shows the results of the virus inactivation experiment for HAV (measurement results by the quantitative PCR method).
- Figure 6 shows the results of the virus inactivation experiment for PPV (measurement results by the infectious titer method).
- the virus inactivation method of the present invention efficiently inactivates viruses present in the space, object or site to be treated by the strong nucleic acid destruction ability and strong penetrating power of the MR gas. Therefore, for example, there is a possibility that the present invention can be applied to a process for killing cells at a specific site (malignant tumor site such as cancer) of an animal.
- this method by implementing this method as an apparatus, for example, while generating MR gas, it is conveyed (supplied) to a specific site of a living body, and the living cells at the specific site are exposed to fresh MR gas.
- a specific site of a living body By realizing such a device, there is a possibility that it can be used for human cancer treatment.
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- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Pest Control & Pesticides (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Virology (AREA)
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- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
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Abstract
Description
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Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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JP2003354286 | 2003-10-14 | ||
JP2003-354286 | 2003-10-14 |
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WO2005035745A1 true WO2005035745A1 (ja) | 2005-04-21 |
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PCT/JP2004/015074 WO2005035745A1 (ja) | 2003-10-14 | 2004-10-13 | ウイルス不活性化方法およびウイルス不活性化装置 |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07231932A (ja) * | 1994-02-22 | 1995-09-05 | France Bed Co Ltd | 被消毒物の消毒方法およびその装置 |
JP2000246119A (ja) * | 1999-03-01 | 2000-09-12 | Hitachi Ltd | 安全キャビネット |
-
2004
- 2004-10-13 WO PCT/JP2004/015074 patent/WO2005035745A1/ja not_active Application Discontinuation
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07231932A (ja) * | 1994-02-22 | 1995-09-05 | France Bed Co Ltd | 被消毒物の消毒方法およびその装置 |
JP2000246119A (ja) * | 1999-03-01 | 2000-09-12 | Hitachi Ltd | 安全キャビネット |
Non-Patent Citations (5)
Title |
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FUJIWARA M. ET AL.: "MR gas no seimitsu iryo kiki no sakkin koka", THE JOURNAL OF JAPANESE MEDICAL INSTRUMENTS, vol. 71, no. 10, 2001, pages 592 - 593, XP002988139 * |
FUKUDA Y.: "MR gas mekkin system jurai no mekkinho no mondai o kaiketsu shita atarashii mekkin system", KENCHIKU SETSUBI TO HAIKAN KOJI, vol. 40, no. 11, 2002, pages 72 - 74, XP002988140 * |
KAWAMURA K. ET AL.: "MR gas sakkin system ni okeru sakkin kiko no kaiseki", CSJ: THE CHEMICAL SOCIETY OF JAPAN, BIOTECHNOLOGY BUKAI SYMPOSIUM KOEN YOSHISHU, vol. 6, 27 September 2002 (2002-09-27), pages 65, XP002988137 * |
KAWAMURA K. ET AL.: "Osen iryo kiki iryo haikibutsu no shoriho no kaihatsu", MR GAS SAKKIN SYSTEM NO OSHIGEN KANKYO TAISAKU, vol. 38, no. 12, 2002, pages 90 - 92, XP002988138 * |
KOMATSU T.: "Shikkan model kaihatsu no tameno kiso kenkyu oyobi model dobutsu no kaihatsu kansen dobutsu jikken shisetsu no biohazard taisaku to anzen hyoka ni kansuru kaihatsu", KANMIN KYODO PROJECT KENKYU HOKOKU HEISEI 7 NENDO DAI 1 BUN'YA, 1996, pages 194 - 203, XP002988136 * |
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