WO2005028619A3 - Synthese, a l'echelle du nanolitre, de biomateriaux en reseaux et criblage de ceux-ci - Google Patents

Synthese, a l'echelle du nanolitre, de biomateriaux en reseaux et criblage de ceux-ci Download PDF

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Publication number
WO2005028619A3
WO2005028619A3 PCT/US2004/030095 US2004030095W WO2005028619A3 WO 2005028619 A3 WO2005028619 A3 WO 2005028619A3 US 2004030095 W US2004030095 W US 2004030095W WO 2005028619 A3 WO2005028619 A3 WO 2005028619A3
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WIPO (PCT)
Prior art keywords
screening
substrate
nanoliter
polymer
scale synthesis
Prior art date
Application number
PCT/US2004/030095
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English (en)
Other versions
WO2005028619A2 (fr
Inventor
Daniel G Anderson
Shulamit Levenberg
Robert S Langer
Original Assignee
Massachusetts Inst Technology
Daniel G Anderson
Shulamit Levenberg
Robert S Langer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/843,707 external-priority patent/US20050019747A1/en
Application filed by Massachusetts Inst Technology, Daniel G Anderson, Shulamit Levenberg, Robert S Langer filed Critical Massachusetts Inst Technology
Priority to EP04788758A priority Critical patent/EP1675943A4/fr
Priority to CA002539670A priority patent/CA2539670A1/fr
Publication of WO2005028619A2 publication Critical patent/WO2005028619A2/fr
Publication of WO2005028619A3 publication Critical patent/WO2005028619A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Reproductive Health (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Une population de cellules épithéliales embryonnaires est produite in vitro à partir de cellules souches embryonnaires. Dans une forme d'exécution, au moins 45 % des cellules expriment de la cytokératine, par exemple, de la cytokératine-7. L'invention concerne un procédé de criblage d'interactions cellule-polymère, procédé comprenant : le dépôt de monomères, sous forme d'une pluralité d'éléments discrets, sur un substrat, provoquant ainsi la polymérisation des monomères déposés, et créant ainsi un réseau d'éléments polymères discrets sur le substrat ; l'incubation du substrat dans un milieu de culture contenant des cellules ; et la caractérisation d'un comportement cellulaire prédéterminé sur chaque élément polymère.
PCT/US2004/030095 2003-09-15 2004-09-15 Synthese, a l'echelle du nanolitre, de biomateriaux en reseaux et criblage de ceux-ci WO2005028619A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP04788758A EP1675943A4 (fr) 2003-09-15 2004-09-15 Synthese, a l'echelle du nanolitre, de biomateriaux en reseaux et criblage de ceux-ci
CA002539670A CA2539670A1 (fr) 2003-09-15 2004-09-15 Synthese, a l'echelle du nanolitre, de biomateriaux en reseaux et criblage de ceux-ci

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US50316503P 2003-09-15 2003-09-15
US60/503,165 2003-09-15
US57018704P 2004-05-12 2004-05-12
US10/843,707 US20050019747A1 (en) 2002-08-07 2004-05-12 Nanoliter-scale synthesis of arrayed biomaterials and screening thereof
US60/570,187 2004-05-12
US10/843,707 2004-05-12

Publications (2)

Publication Number Publication Date
WO2005028619A2 WO2005028619A2 (fr) 2005-03-31
WO2005028619A3 true WO2005028619A3 (fr) 2005-09-09

Family

ID=34381975

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/030095 WO2005028619A2 (fr) 2003-09-15 2004-09-15 Synthese, a l'echelle du nanolitre, de biomateriaux en reseaux et criblage de ceux-ci

Country Status (3)

Country Link
EP (1) EP1675943A4 (fr)
CA (1) CA2539670A1 (fr)
WO (1) WO2005028619A2 (fr)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2476756A1 (fr) 2005-06-15 2012-07-18 Massachusetts Institute of Technology Lipides contenant des amines et utilisations associées
EP2064375B1 (fr) * 2006-06-06 2012-11-21 Société de Commercialisation des Produits de la Recherche Appliquée - SOCPRA- Sciences et Génie s.e.c. Supports d'essai comportant un support de polyethylene glycol, ledit support fixe provenant d'une solution de polyethylene glycol dans des conditions de point de trouble (solvent theta)
EP2240536B1 (fr) 2008-01-30 2019-09-04 Asterias Biotherapeutics, Inc. Surfaces synthétiques pour la culture de cellules progénitrices d'oligodendrocytes issues de cellules souches
US8329469B2 (en) * 2008-01-30 2012-12-11 Geron Corporation Swellable (meth)acrylate surfaces for culturing cells in chemically defined media
CA2712891A1 (fr) * 2008-01-30 2009-08-06 Corning Incorporated Surfaces synthetiques pour la culture de cardiomyocytes issus de cellules souches
ATE555191T1 (de) * 2008-01-30 2012-05-15 Geron Corp Synthetische oberflächen zur kultivierung von zellen in chemisch definierten medien
WO2009099539A2 (fr) * 2008-01-30 2009-08-13 Corning Incorporated Surfaces de (méth)acrylate de culture cellulaire, et procédé de fabrication et d'utilisation desdites surfaces
MX353900B (es) 2008-11-07 2018-02-01 Massachusetts Inst Technology Lipidoides de aminoalcohol y usos de los mismos.
WO2011014845A1 (fr) * 2009-07-31 2011-02-03 Nanoink, Inc. Système de criblage destiné à identifier des motifs sur des surfaces de substrats pour induire la différenciation des cellules souches et pour produire une population homogène d'un type de cellule recherché
RS58405B1 (sr) 2009-12-01 2019-04-30 Translate Bio Inc Stereoidni derivati za isporuku irnk u humanim genetskim oboljenjima
EP2609135A4 (fr) 2010-08-26 2015-05-20 Massachusetts Inst Technology Poly(bêta-amino-alcools), leur préparation et utilisations de ceux-ci
WO2012135025A2 (fr) 2011-03-28 2012-10-04 Massachusetts Institute Of Technology Lipomères conjugués et utilisations associées
RS59037B1 (sr) 2011-06-08 2019-08-30 Translate Bio Inc Kompozicije lipidnih nanočestica i postupci za isporuku irnk
EP2859102A4 (fr) 2012-06-08 2016-05-11 Shire Human Genetic Therapies Polynucléotides résistant à la nucléase et leurs utilisations
WO2014028487A1 (fr) 2012-08-13 2014-02-20 Massachusetts Institute Of Technology Lipidoïdes contenant des amines et leurs utilisations
AU2014236396A1 (en) 2013-03-14 2015-08-13 Shire Human Genetic Therapies, Inc. Methods for purification of messenger RNA
EP3446712A1 (fr) 2013-03-14 2019-02-27 Translate Bio Ma, Inc. Compositions d'arnm cftr et procédés et utilisations associés
WO2014179562A1 (fr) 2013-05-01 2014-11-06 Massachusetts Institute Of Technology Dérivés de 1,3,5-triazinane-2,4,6-trione et leurs utilisations
US11224642B2 (en) 2013-10-22 2022-01-18 Translate Bio, Inc. MRNA therapy for argininosuccinate synthetase deficiency
EA034103B1 (ru) 2013-10-22 2019-12-27 Транслейт Био, Инк. СПОСОБ ЛЕЧЕНИЯ ФЕНИЛКЕТОНУРИИ С ПРИМЕНЕНИЕМ мРНК
MX2016005238A (es) 2013-10-22 2016-08-12 Shire Human Genetic Therapies Formulaciones de lipidos para la administracion de acido ribonucleico mensajero.
BR112016024632A2 (pt) 2014-04-25 2018-01-30 Shire Human Genetic Therapies métodos de purificação de rna mensageiro
EP3148552B1 (fr) 2014-05-30 2019-07-31 Translate Bio, Inc. Lipides biodégradables pour l'administration d'acides nucléiques
AU2015279968B2 (en) 2014-06-24 2019-11-14 Translate Bio, Inc. Stereochemically enriched compositions for delivery of nucleic acids
WO2016004202A1 (fr) 2014-07-02 2016-01-07 Massachusetts Institute Of Technology Lipidoïdes dérivés de polyamine-acide gras et leurs utilisations
GB201416078D0 (en) * 2014-09-11 2014-10-29 Univ Nottingham Cell culture substrate
MX2019010155A (es) 2017-02-27 2020-12-10 Translate Bio Inc Arnm de cftr optimizado por codón novedoso.
MA49138A (fr) 2017-05-16 2020-03-25 Translate Bio Inc Traitement de la fibrose kystique par administration d'arnm à codons optimisés codant pour la cftr
AU2019325702A1 (en) 2018-08-24 2021-02-25 Translate Bio, Inc. Methods for purification of messenger RNA

Citations (1)

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Publication number Priority date Publication date Assignee Title
US20020142304A1 (en) * 2001-03-09 2002-10-03 Anderson Daniel G. Uses and methods of making microarrays of polymeric biomaterials

Family Cites Families (1)

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Publication number Priority date Publication date Assignee Title
FR2825370A1 (fr) * 2001-05-31 2002-12-06 Inst Nat Sante Rech Med Keratinocytes obtenus a partir de cellules souches embryonnaires de mammiferes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020142304A1 (en) * 2001-03-09 2002-10-03 Anderson Daniel G. Uses and methods of making microarrays of polymeric biomaterials

Non-Patent Citations (2)

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Title
LI Y. ET AL: "Plasticity of the urothelial phenotype: effects of gastro-intestinal mesenchyme/stroma and implications for urinary tract reconstruction", DIFFERENTIATION, vol. 66, 2000, pages 126 - 135, XP002989066 *
See also references of EP1675943A4 *

Also Published As

Publication number Publication date
EP1675943A2 (fr) 2006-07-05
EP1675943A4 (fr) 2007-12-05
CA2539670A1 (fr) 2005-03-31
WO2005028619A2 (fr) 2005-03-31

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