WO2004088307A3 - Verfahren zum nachweis der expression rekombinanter proteine an einer zelloberfläche und zur immobilisierung und aufreinigung von zellen und proteinen - Google Patents

Verfahren zum nachweis der expression rekombinanter proteine an einer zelloberfläche und zur immobilisierung und aufreinigung von zellen und proteinen Download PDF

Info

Publication number
WO2004088307A3
WO2004088307A3 PCT/DE2004/000506 DE2004000506W WO2004088307A3 WO 2004088307 A3 WO2004088307 A3 WO 2004088307A3 DE 2004000506 W DE2004000506 W DE 2004000506W WO 2004088307 A3 WO2004088307 A3 WO 2004088307A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
expression
cell surface
immobilization
purification
Prior art date
Application number
PCT/DE2004/000506
Other languages
English (en)
French (fr)
Other versions
WO2004088307A2 (de
Inventor
Joachim Jose
Steffen Handel
Original Assignee
Univ Saarland
Joachim Jose
Steffen Handel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Saarland, Joachim Jose, Steffen Handel filed Critical Univ Saarland
Publication of WO2004088307A2 publication Critical patent/WO2004088307A2/de
Publication of WO2004088307A3 publication Critical patent/WO2004088307A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Das Verfahren zum Nachweis der Expression eines rekombinanten Polypeptids in/an/auf der Zelloberfläche seiner Wirtszelle zeichnet sich dadurch aus, daß man (a) das rekombinante Polypeptid derart konstruiert, daß es in einem dem äußeren Medium der Wirtszelle zugänglichen Abschnitt wenigstens einmal die Aminosäure Cystein aufweist, daß man (b) die Wirtszelle einem äußeren Medium aussetzt, welches eine Markersubstanz enthält, die spezifisch an Cystein bindet, daß man (c) die Bindungsreaktion zwischen Cystein und der Markersubstanz herbeiführt, und daß man (d) die Wirtszelle anschließend einem Prozess zur Detektion der Cystein-Marker­Verbindungen unterwirft.
PCT/DE2004/000506 2003-03-28 2004-03-13 Verfahren zum nachweis der expression rekombinanter proteine an einer zelloberfläche und zur immobilisierung und aufreinigung von zellen und proteinen WO2004088307A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10314413.7 2003-03-28
DE10314413.7A DE10314413B4 (de) 2003-03-28 2003-03-28 Verfahren zum Nachweis der Expression rekombinanter Proteine an einer Zelloberfläche und zur Immobilisierung und Aufreinigung von Zellen und Proteinen

Publications (2)

Publication Number Publication Date
WO2004088307A2 WO2004088307A2 (de) 2004-10-14
WO2004088307A3 true WO2004088307A3 (de) 2004-11-25

Family

ID=33103136

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2004/000506 WO2004088307A2 (de) 2003-03-28 2004-03-13 Verfahren zum nachweis der expression rekombinanter proteine an einer zelloberfläche und zur immobilisierung und aufreinigung von zellen und proteinen

Country Status (2)

Country Link
DE (1) DE10314413B4 (de)
WO (1) WO2004088307A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9482663B2 (en) 2009-09-09 2016-11-01 Autodisplay Biotech Gmbh Method for binding a recombinant polypeptide to a carrier
EP2619306B1 (de) * 2010-09-23 2015-05-20 Autodisplay Biotech GmbH Zelloberflächenpräsentation von metall-pophyrin oder -flavin enthaltenen polypeptiden

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997035022A1 (de) * 1996-03-15 1997-09-25 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Berlin Exportsysteme für rekombinante proteine

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4344350C2 (de) * 1993-12-23 1995-09-21 Max Planck Gesellschaft Bakterien zur Herstellung stabiler Fusionsproteine und Verfahren zu deren Nachweis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997035022A1 (de) * 1996-03-15 1997-09-25 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Berlin Exportsysteme für rekombinante proteine

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DAUGHERTY PATRICK S ET AL: "Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface", PROTEIN ENGINEERING, vol. 12, no. 7, July 1999 (1999-07-01), pages 613 - 621, XP002296794, ISSN: 0269-2139 *
GEORGIOU G ET AL: "DISPLAY OF HETEROLOGOUS PROTEINS ON THE SURFACE OF MICROORGANISMS: FROM THE SCREENING OF COMBINATORIAL LIBRARIES TO LIVE RECOMBINANT VACCINES", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 15, January 1997 (1997-01-01), pages 29 - 34, XP002950292, ISSN: 1087-0156 *
JOSE JOACHIM ET AL: "Cellular surface display of dimeric Adx and whole cell P450-mediated steroid synthesis on E. coli", JOURNAL OF BIOTECHNOLOGY, vol. 95, no. 3, 23 May 2002 (2002-05-23), pages 257 - 268, XP002296795, ISSN: 0168-1656 *
KRATZ FELIX ET AL: "A novel macromolecular prodrug concept exploiting endogenous serum albumin as a drug carrier for cancer chemotherapy", JOURNAL OF MEDICINAL CHEMISTRY, vol. 43, no. 7, 6 April 2000 (2000-04-06), pages 1253 - 1256, XP002296797, ISSN: 0022-2623 *
MAURER JOCHEN ET AL: "Autodisplay: One component system for efficient surface display and release of soluble recombinant proteins from Escherichia coli", JOURNAL OF BACTERIOLOGY, vol. 179, no. 3, 1997, pages 794 - 804, XP002296793, ISSN: 0021-9193 *
SCHULTHEISS EVA ET AL: "Functional esterase surface display by the autotransporter pathway in Escherichia coli", JOURNAL OF MOLECULAR CATALYSIS B ENZYMATIC, vol. 18, no. 1-3, 13 September 2002 (2002-09-13), pages 89 - 97, XP002296796, ISSN: 1381-1177 *

Also Published As

Publication number Publication date
WO2004088307A2 (de) 2004-10-14
DE10314413B4 (de) 2015-08-06
DE10314413A1 (de) 2004-12-09

Similar Documents

Publication Publication Date Title
WO2004048592A3 (en) Bgl7 beta-glucosidase and nucleic acids encoding the same
WO2004043980A3 (en) Bgl6 beta-glucosidase and nucleic acids encoding the same
WO2008008975A8 (en) Refolding of recombinant proteins
WO2003052054A3 (en) Bgl5 beta-glucosidase and nucleic acids encoding the same
WO2003027306A3 (en) Bgl3 beta-glucosidase and nucleic acids encoding the same
WO2002025287A3 (en) Detection of peptides
WO2007018619A3 (en) Chimeric therapeutic agents
WO2004022593A3 (en) Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules
WO2003052118A3 (en) Bgl4 beta-glucosidase and nucleic acids encoding the same
WO2003010176A3 (en) Protein-nucleic acid complexes
WO2004074310A3 (en) Mycobacterial diagnostics
WO2003052055A3 (en) Egvii endoglucanase and nucleic acids encoding the same
WO2003052056A3 (en) Egviii endoglucanase and nucleic acids encoding the same
WO2003074992A3 (en) Phosphorylated proteins and uses related thereto
WO2005116660A3 (en) Methods for making and using mass tag standards for quantitative proteomics
WO2009054435A1 (ja) 増強されたエフェクター機能を有するポリペプチド
WO2007051002A3 (en) Activated split-polypeptides and methods for their production and use
WO2007064844A3 (en) Anti-viral griffithsin compounds, compositions, and methods of use
WO2006021584A3 (en) Purification of factor xiii polypeptides from biological materials
DE60313866D1 (de) Polypeptide mit alpha-h alpha amino acid amide racemase aktivitität und nukleinsäuren kodierend dafür
WO2003074706A1 (fr) Proteine ou polypeptide ayant une activite de synthase de type substance lacrymogene, adn codant la proteine ou le polypeptide, procede d'obtention de ladite proteine ou dudit polypeptide, et molecule d'acides nucleiques inhibant la translation de l'arn messager concernant la proteine ou le peptide
AU2003260392A1 (en) Method for enriching a prokaryotic dna
WO2005001099A8 (en) Protein expression system
WO2004088307A3 (de) Verfahren zum nachweis der expression rekombinanter proteine an einer zelloberfläche und zur immobilisierung und aufreinigung von zellen und proteinen
WO2003060524A3 (en) Enzyme/chemical reactor based protein processing method for proteomics analysis by mass spectrometry

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase