WO2004076669A3 - Cystein-substituierte fad-abhängige sulfhydryloxidasen - Google Patents

Cystein-substituierte fad-abhängige sulfhydryloxidasen Download PDF

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Publication number
WO2004076669A3
WO2004076669A3 PCT/DE2004/000308 DE2004000308W WO2004076669A3 WO 2004076669 A3 WO2004076669 A3 WO 2004076669A3 DE 2004000308 W DE2004000308 W DE 2004000308W WO 2004076669 A3 WO2004076669 A3 WO 2004076669A3
Authority
WO
WIPO (PCT)
Prior art keywords
fad
cysteine
substituted
relates
sulfhydryl oxidases
Prior art date
Application number
PCT/DE2004/000308
Other languages
English (en)
French (fr)
Other versions
WO2004076669A2 (de
Inventor
Goetz Hofhaus
Thomas Lisowsky
Original Assignee
Univ Duesseldorf H Heine
Goetz Hofhaus
Thomas Lisowsky
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Duesseldorf H Heine, Goetz Hofhaus, Thomas Lisowsky filed Critical Univ Duesseldorf H Heine
Publication of WO2004076669A2 publication Critical patent/WO2004076669A2/de
Publication of WO2004076669A3 publication Critical patent/WO2004076669A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P11/00—Preparation of sulfur-containing organic compounds

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft rekombinante, FAD-abhängige Sulfhydryloxidasen, in denen min-destens ein Cysteinrest derart mutiert wurde, dass dies zu einer Veränderung des Absorptionssmaximums im Vergleich zum Wildtypprotein führt, die korrespondierenden Nukleinsäuremoleküle sowie ein Verfahren zu ihrer Herstellung. Ferner betrifft die Erfindung die Verwendung dieser Proteine als Biokatalysatoren für die Synthese von Disulfidbrückenbindungen und deren Übertragung auf Substrate sowie als regulierbare Indikatoren für Redoxreaktionen.
PCT/DE2004/000308 2003-02-28 2004-02-19 Cystein-substituierte fad-abhängige sulfhydryloxidasen WO2004076669A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10309169.6 2003-02-28
DE10309169A DE10309169B4 (de) 2003-02-28 2003-02-28 Rekombinante, FAD-abhängige Sulfhydryloxidasen, Verfahren zu deren Herstellung und deren Verwendung

Publications (2)

Publication Number Publication Date
WO2004076669A2 WO2004076669A2 (de) 2004-09-10
WO2004076669A3 true WO2004076669A3 (de) 2004-11-18

Family

ID=32864058

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2004/000308 WO2004076669A2 (de) 2003-02-28 2004-02-19 Cystein-substituierte fad-abhängige sulfhydryloxidasen

Country Status (2)

Country Link
DE (1) DE10309169B4 (de)
WO (1) WO2004076669A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004028778A1 (de) * 2004-06-16 2006-01-05 Heinrich-Heine-Universität Düsseldorf Regulatorische Oligopeptide und deren Verwendung
EP2103220B1 (de) 2008-03-17 2018-09-26 Stern Enzym GmbH & Co. KG Verfahren zur Herstellung von laminiertem Teig, Sulfhydryl-Oxidase enthaltend
DE102009034601A1 (de) 2009-07-24 2011-01-27 Stern-Enzym Gmbh & Co. Kg Erhöhung der Viskosität eihaltiger Lebensmittel
GB201018664D0 (en) * 2010-11-05 2010-12-22 Msd Biolog Uk Ltd Expression process

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHEN XIAOXIAO ET AL: "The potentiation role of hepatopoietin on activator protein-1 is dependent on its sulfhydryl oxidase activity.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 49, 5 December 2003 (2003-12-05), pages 49022 - 49030, XP002291355, ISSN: 0021-9258 *
DATABASE UNIPROT 1 August 1992 (1992-08-01), "ERV1 proein; mitochondrial precursor (14 kDa regulatory protein)", XP002291356, Database accession no. P27882 *
DATABASE UNIPROT 1 March 2003 (2003-03-01), "Hypothetical protein At1g49880/F10F5_3 (At1g49880) (Mitochondrial sulfhydryl oxidase Erv1p)", XP002291357, Database accession no. Q8GXX0 *
HOFHAUS GOETZ ET AL: "The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 270, no. 7, April 2003 (2003-04-01), pages 1528 - 1535, XP002291353, ISSN: 0014-2956 *
LEE^A J-E ET AL: "Erv1p from Saccharomyces cerevisiae is a FAD-linked sulfhydryl oxidase", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 477, no. 1-2, 14 July 2000 (2000-07-14), pages 62 - 66, XP004337352, ISSN: 0014-5793 *
LISOWSKY T ET AL: "Mammalian augmenter of liver regeneration protein is a sulfhydryl oxidase", DIGESTIVE AND LIVER DISEASE, vol. 33, no. 2, March 2001 (2001-03-01), pages 173 - 180, XP002291352 *
WU CHIA-KUEI ET AL: "The crystal structure of augmenter of liver regeneration: A mammalian FAD-dependent sulfhydryl oxidase.", PROTEIN SCIENCE, vol. 12, no. 5, May 2003 (2003-05-01), pages 1109 - 1118, XP002291354, ISSN: 0961-8368 *

Also Published As

Publication number Publication date
WO2004076669A2 (de) 2004-09-10
DE10309169A1 (de) 2004-09-16
DE10309169B4 (de) 2010-04-08

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