WO2004076669A3 - Cystein-substituierte fad-abhängige sulfhydryloxidasen - Google Patents
Cystein-substituierte fad-abhängige sulfhydryloxidasen Download PDFInfo
- Publication number
- WO2004076669A3 WO2004076669A3 PCT/DE2004/000308 DE2004000308W WO2004076669A3 WO 2004076669 A3 WO2004076669 A3 WO 2004076669A3 DE 2004000308 W DE2004000308 W DE 2004000308W WO 2004076669 A3 WO2004076669 A3 WO 2004076669A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- fad
- cysteine
- substituted
- relates
- sulfhydryl oxidases
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P11/00—Preparation of sulfur-containing organic compounds
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Die Erfindung betrifft rekombinante, FAD-abhängige Sulfhydryloxidasen, in denen min-destens ein Cysteinrest derart mutiert wurde, dass dies zu einer Veränderung des Absorptionssmaximums im Vergleich zum Wildtypprotein führt, die korrespondierenden Nukleinsäuremoleküle sowie ein Verfahren zu ihrer Herstellung. Ferner betrifft die Erfindung die Verwendung dieser Proteine als Biokatalysatoren für die Synthese von Disulfidbrückenbindungen und deren Übertragung auf Substrate sowie als regulierbare Indikatoren für Redoxreaktionen.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10309169.6 | 2003-02-28 | ||
DE10309169A DE10309169B4 (de) | 2003-02-28 | 2003-02-28 | Rekombinante, FAD-abhängige Sulfhydryloxidasen, Verfahren zu deren Herstellung und deren Verwendung |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2004076669A2 WO2004076669A2 (de) | 2004-09-10 |
WO2004076669A3 true WO2004076669A3 (de) | 2004-11-18 |
Family
ID=32864058
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DE2004/000308 WO2004076669A2 (de) | 2003-02-28 | 2004-02-19 | Cystein-substituierte fad-abhängige sulfhydryloxidasen |
Country Status (2)
Country | Link |
---|---|
DE (1) | DE10309169B4 (de) |
WO (1) | WO2004076669A2 (de) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102004028778A1 (de) * | 2004-06-16 | 2006-01-05 | Heinrich-Heine-Universität Düsseldorf | Regulatorische Oligopeptide und deren Verwendung |
EP2103220B1 (de) | 2008-03-17 | 2018-09-26 | Stern Enzym GmbH & Co. KG | Verfahren zur Herstellung von laminiertem Teig, Sulfhydryl-Oxidase enthaltend |
DE102009034601A1 (de) | 2009-07-24 | 2011-01-27 | Stern-Enzym Gmbh & Co. Kg | Erhöhung der Viskosität eihaltiger Lebensmittel |
GB201018664D0 (en) * | 2010-11-05 | 2010-12-22 | Msd Biolog Uk Ltd | Expression process |
-
2003
- 2003-02-28 DE DE10309169A patent/DE10309169B4/de not_active Expired - Fee Related
-
2004
- 2004-02-19 WO PCT/DE2004/000308 patent/WO2004076669A2/de active Application Filing
Non-Patent Citations (7)
Title |
---|
CHEN XIAOXIAO ET AL: "The potentiation role of hepatopoietin on activator protein-1 is dependent on its sulfhydryl oxidase activity.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 49, 5 December 2003 (2003-12-05), pages 49022 - 49030, XP002291355, ISSN: 0021-9258 * |
DATABASE UNIPROT 1 August 1992 (1992-08-01), "ERV1 proein; mitochondrial precursor (14 kDa regulatory protein)", XP002291356, Database accession no. P27882 * |
DATABASE UNIPROT 1 March 2003 (2003-03-01), "Hypothetical protein At1g49880/F10F5_3 (At1g49880) (Mitochondrial sulfhydryl oxidase Erv1p)", XP002291357, Database accession no. Q8GXX0 * |
HOFHAUS GOETZ ET AL: "The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 270, no. 7, April 2003 (2003-04-01), pages 1528 - 1535, XP002291353, ISSN: 0014-2956 * |
LEE^A J-E ET AL: "Erv1p from Saccharomyces cerevisiae is a FAD-linked sulfhydryl oxidase", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 477, no. 1-2, 14 July 2000 (2000-07-14), pages 62 - 66, XP004337352, ISSN: 0014-5793 * |
LISOWSKY T ET AL: "Mammalian augmenter of liver regeneration protein is a sulfhydryl oxidase", DIGESTIVE AND LIVER DISEASE, vol. 33, no. 2, March 2001 (2001-03-01), pages 173 - 180, XP002291352 * |
WU CHIA-KUEI ET AL: "The crystal structure of augmenter of liver regeneration: A mammalian FAD-dependent sulfhydryl oxidase.", PROTEIN SCIENCE, vol. 12, no. 5, May 2003 (2003-05-01), pages 1109 - 1118, XP002291354, ISSN: 0961-8368 * |
Also Published As
Publication number | Publication date |
---|---|
DE10309169A1 (de) | 2004-09-16 |
WO2004076669A2 (de) | 2004-09-10 |
DE10309169B4 (de) | 2010-04-08 |
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