WO2004058989A3 - Transcription dependante de la cible - Google Patents

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Publication number
WO2004058989A3
WO2004058989A3 PCT/US2003/040912 US0340912W WO2004058989A3 WO 2004058989 A3 WO2004058989 A3 WO 2004058989A3 US 0340912 W US0340912 W US 0340912W WO 2004058989 A3 WO2004058989 A3 WO 2004058989A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
transcription
kits
compositions
nucleic acid
Prior art date
Application number
PCT/US2003/040912
Other languages
English (en)
Other versions
WO2004058989A2 (fr
Original Assignee
Epict Technologies
Dahl Gary A
Jendrisak Jerome J
Meis Judy E
Vaidyanathan Ramesh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epict Technologies, Dahl Gary A, Jendrisak Jerome J, Meis Judy E, Vaidyanathan Ramesh filed Critical Epict Technologies
Priority to AU2003301219A priority Critical patent/AU2003301219A1/en
Priority to EP03814301A priority patent/EP1583840A4/fr
Publication of WO2004058989A2 publication Critical patent/WO2004058989A2/fr
Publication of WO2004058989A3 publication Critical patent/WO2004058989A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne de nouvelles méthodes, compositions et trousses comprenant des sondes cibles monopartites ou bipartites et une ARN polymérase destinées à détecter et quantifier des substances à analyser comprenant une ou plusieurs séquences d'acide nucléique, y compris des séquences cibles qui diffèrent seulement d'un nucléotide; ou à détecter et quantifier des substances à analyser exemptes d'acide nucléique, par détection d'une étiquette de séquence cible associée à une substance de liaison aux substances à analyser. Le procédé de l'invention, appelé 'transcription dépendante de la cible', comprend un traitement de renaturation, un processus de ligature d'ADN, un processus de transcription, et un processus de détection. L'invention concerne en outre de nouvelles méthodes, compositions et trousses destinées à amplifier l'ARN, y compris par transcription inverse par déplacement de brins et transcription inverse par cercle roulant.
PCT/US2003/040912 2002-12-23 2003-12-23 Transcription dependante de la cible WO2004058989A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003301219A AU2003301219A1 (en) 2002-12-23 2003-12-23 Target-dependent transcription
EP03814301A EP1583840A4 (fr) 2002-12-23 2003-12-23 Transcription dependante de la cible

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43606202P 2002-12-23 2002-12-23
US60/436,062 2002-12-23

Publications (2)

Publication Number Publication Date
WO2004058989A2 WO2004058989A2 (fr) 2004-07-15
WO2004058989A3 true WO2004058989A3 (fr) 2007-10-18

Family

ID=32682331

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/040912 WO2004058989A2 (fr) 2002-12-23 2003-12-23 Transcription dependante de la cible

Country Status (3)

Country Link
EP (1) EP1583840A4 (fr)
AU (1) AU2003301219A1 (fr)
WO (1) WO2004058989A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20080047355A (ko) * 2005-07-06 2008-05-28 바이오칩 이노베이션즈 피티와이 리미티드 표적 핵산 서열 분석 방법 및 키트
AU2012304328B2 (en) 2011-09-09 2017-07-20 The Board Of Trustees Of The Leland Stanford Junior University Methods for obtaining a sequence
CN106801050B (zh) * 2017-02-20 2020-01-14 广州永诺生物科技有限公司 一种环状rna高通量测序文库的构建方法及其试剂盒
GB201904081D0 (en) * 2019-03-25 2019-05-08 Feyrer Hannes Method and products for producing RNA molecules

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5683888A (en) * 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5700667A (en) * 1993-09-20 1997-12-23 Regents Of The University Of Colorado, The Strategy for the production of RNA from immobilized templates
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6593086B2 (en) * 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5683888A (en) * 1989-07-22 1997-11-04 University Of Wales College Of Medicine Modified bioluminescent proteins and their use
US5700667A (en) * 1993-09-20 1997-12-23 Regents Of The University Of Colorado, The Strategy for the production of RNA from immobilized templates
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of EP1583840A4 *
TYAGI S. ET AL.: "Extremely sensitive, background-free gene detection using binary probes and Qb replicase", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 93, no. 11, May 1996 (1996-05-01), pages 5395 - 5400, XP000600188 *

Also Published As

Publication number Publication date
AU2003301219A8 (en) 2004-07-22
EP1583840A4 (fr) 2009-03-18
EP1583840A2 (fr) 2005-10-12
WO2004058989A2 (fr) 2004-07-15
AU2003301219A1 (en) 2004-07-22

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