WO2004058391A3 - Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren - Google Patents

Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren Download PDF

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Publication number
WO2004058391A3
WO2004058391A3 PCT/EP2003/014822 EP0314822W WO2004058391A3 WO 2004058391 A3 WO2004058391 A3 WO 2004058391A3 EP 0314822 W EP0314822 W EP 0314822W WO 2004058391 A3 WO2004058391 A3 WO 2004058391A3
Authority
WO
WIPO (PCT)
Prior art keywords
protective groups
synthesis
stage protective
photoactivatable
biopolymers
Prior art date
Application number
PCT/EP2003/014822
Other languages
English (en)
French (fr)
Other versions
WO2004058391A2 (de
Inventor
Barbro Beijer
Ramon Gueimil
Matthias Scheffler
Original Assignee
Febit Ag
Barbro Beijer
Ramon Gueimil
Matthias Scheffler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10260591A external-priority patent/DE10260591A1/de
Priority claimed from DE10260592A external-priority patent/DE10260592A1/de
Application filed by Febit Ag, Barbro Beijer, Ramon Gueimil, Matthias Scheffler filed Critical Febit Ag
Priority to US10/540,396 priority Critical patent/US7737089B2/en
Priority to AU2003290117A priority patent/AU2003290117A1/en
Priority to EP03782479A priority patent/EP1585830A2/de
Publication of WO2004058391A2 publication Critical patent/WO2004058391A2/de
Publication of WO2004058391A3 publication Critical patent/WO2004058391A3/de

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
    • C40B50/18—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support using a particular method of attachment to the solid support
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00711—Light-directed synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Nanotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Structural Engineering (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Materials Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Composite Materials (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Indole Compounds (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur Synthese von Biopolymeren durch schrittweisen Aufbau aus geschützten Synthesebausteinen, die zweistufige Schutzgruppen tragen. Die zweistufigen Schutzgruppen werden durch einen ersten Belichtungsschritt aktiviert und einen anschliessenden chemischen Behandlungsschritt abgespalten. Es werden photoaktivierbare Komponenten verwendet, die über intramolekulare Triplettsensibilisatoren den Aktivierungsprozess erheblich beschleunigen oder/und über Fluoreszenzeigenschaften verfügen. Die zweistufigen Schutzgruppen können insbesondere im Rahmen einer Qualitätskontrolle Anwendung finden.
PCT/EP2003/014822 2002-12-23 2003-12-23 Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren WO2004058391A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/540,396 US7737089B2 (en) 2002-12-23 2003-12-23 Photoactivatable two-stage protective groups for the synthesis of biopolymers
AU2003290117A AU2003290117A1 (en) 2002-12-23 2003-12-23 Photoactivatable two-stage protective groups for the synthesis of biopolymers
EP03782479A EP1585830A2 (de) 2002-12-23 2003-12-23 Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE10260591A DE10260591A1 (de) 2002-12-23 2002-12-23 Photoaktivierbare zweistufige Schutzgruppen für die Synthese von Biopolymeren
DE10260592.0 2002-12-23
DE10260591.2 2002-12-23
DE10260592A DE10260592A1 (de) 2002-12-23 2002-12-23 Intramolekular triplettsensibilisierte o-Nitrophenylethyl-Photoschutzgruppen

Publications (2)

Publication Number Publication Date
WO2004058391A2 WO2004058391A2 (de) 2004-07-15
WO2004058391A3 true WO2004058391A3 (de) 2004-08-26

Family

ID=32683475

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/EP2003/014823 WO2004058392A2 (de) 2002-12-23 2003-12-23 Intramolekular triplettsensibilisierte o-nitrophenylethyl-photoschutzgruppen
PCT/EP2003/014822 WO2004058391A2 (de) 2002-12-23 2003-12-23 Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/014823 WO2004058392A2 (de) 2002-12-23 2003-12-23 Intramolekular triplettsensibilisierte o-nitrophenylethyl-photoschutzgruppen

Country Status (4)

Country Link
US (1) US7737089B2 (de)
EP (1) EP1585830A2 (de)
AU (2) AU2003290117A1 (de)
WO (2) WO2004058392A2 (de)

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AU2003290117A1 (en) * 2002-12-23 2004-07-22 Febit Biotech Gmbh Photoactivatable two-stage protective groups for the synthesis of biopolymers
DE102005027667A1 (de) * 2005-06-15 2006-12-28 Febit Biotech Gmbh Verfahren zur Qualitätskontrolle funktionalisierter Oberflächen
GB2533173A (en) 2013-08-05 2016-06-15 Twist Bioscience Corp De Novo synthesized gene libraries
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
IL258164B (en) 2015-09-18 2022-09-01 Twist Bioscience Corp Methods to regulate the activity of proteins and cells and a method for the production of nucleic acids
KR20180058772A (ko) 2015-09-22 2018-06-01 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
JP6871364B2 (ja) 2016-09-21 2021-05-12 ツイスト バイオサイエンス コーポレーション 核酸に基づくデータ保存
US10907274B2 (en) 2016-12-16 2021-02-02 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CA3054303A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
AU2018284227B2 (en) 2017-06-12 2024-05-02 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
EA202090562A1 (ru) 2017-09-11 2020-08-10 Твист Байосайенс Корпорейшн Связывающие gpcr белки и их синтез
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
EP3735459A4 (de) 2018-01-04 2021-10-06 Twist Bioscience Corporation Digitale informationsspeicherung auf dna-basis
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
SG11202109283UA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for antibody optimization
CN113766930A (zh) 2019-02-26 2021-12-07 特韦斯特生物科学公司 Glp1受体的变异核酸文库
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066259A1 (en) * 1999-05-05 2000-11-09 Ut-Battelle, Llc Method and apparatus for combinatorial chemistry
WO2002020150A2 (en) * 2000-09-11 2002-03-14 Affymetrix, Inc. Photocleavable protecting groups
WO2002062815A1 (de) * 2001-02-05 2002-08-15 Febit Ag Hybrid-schutzgruppe
DE10122357A1 (de) * 2001-05-09 2002-11-14 Febit Ferrarius Biotech Gmbh Hybridverfahren zur Herstellung von Trägern für die Analytbestimmung
WO2003004510A1 (de) * 2001-07-03 2003-01-16 Febit Ag Zweistufige schutzgruppen für die synthese von biopolymeren

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4444996A1 (de) * 1994-12-16 1996-06-20 Wolfgang Prof Dr Dr Pfleiderer Nucleosid-Derivate mit photolabilen Schutzgruppen
DE19620170A1 (de) * 1996-05-20 1997-11-27 Wolfgang Prof Dr Dr Pfleiderer Nucleosid-Derivate mit photolabilen Schutzgruppen
US6756492B1 (en) * 1999-04-08 2004-06-29 Deutsches Krebsforschungszentrum Stiftund Des Offentlichen Rechts Nucleoside derivatives with photo-unstable protective groups
DE10105079A1 (de) * 2001-02-05 2002-08-08 Febit Ferrarius Biotech Gmbh Fotolabile Schutzgruppen für die Synthese von Biopolymeren
AU2003290117A1 (en) * 2002-12-23 2004-07-22 Febit Biotech Gmbh Photoactivatable two-stage protective groups for the synthesis of biopolymers

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066259A1 (en) * 1999-05-05 2000-11-09 Ut-Battelle, Llc Method and apparatus for combinatorial chemistry
WO2002020150A2 (en) * 2000-09-11 2002-03-14 Affymetrix, Inc. Photocleavable protecting groups
WO2002062815A1 (de) * 2001-02-05 2002-08-15 Febit Ag Hybrid-schutzgruppe
DE10122357A1 (de) * 2001-05-09 2002-11-14 Febit Ferrarius Biotech Gmbh Hybridverfahren zur Herstellung von Trägern für die Analytbestimmung
WO2003004510A1 (de) * 2001-07-03 2003-01-16 Febit Ag Zweistufige schutzgruppen für die synthese von biopolymeren

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HAPP E ET AL: "NEW TRITYL-BASED PROTECTING GROUPS WITH A MILD TWO-STEP REMOVAL", NUCLEOSIDES & NUCLEOTIDES, DEKKER, NEW YORK,NY,, US, vol. 7, no. 5/6, 1988, pages 813 - 816, XP008011020, ISSN: 0732-8311 *

Also Published As

Publication number Publication date
WO2004058391A2 (de) 2004-07-15
US7737089B2 (en) 2010-06-15
WO2004058392A3 (de) 2004-08-26
AU2003298235A1 (en) 2004-07-22
US20060111564A1 (en) 2006-05-25
AU2003290117A1 (en) 2004-07-22
AU2003290117A8 (en) 2004-07-22
EP1585830A2 (de) 2005-10-19
WO2004058392A2 (de) 2004-07-15
AU2003298235A8 (en) 2004-07-22

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