WO2004041856A3 - Crystal structures of bacterial inorganic pyrophosphatases - Google Patents

Crystal structures of bacterial inorganic pyrophosphatases

Info

Publication number
WO2004041856A3
WO2004041856A3 PCT/CA2003/001704 CA0301704W WO2004041856A3 WO 2004041856 A3 WO2004041856 A3 WO 2004041856A3 CA 0301704 W CA0301704 W CA 0301704W WO 2004041856 A3 WO2004041856 A3 WO 2004041856A3
Authority
WO
WIPO (PCT)
Prior art keywords
crystal structures
bacterial
inorganic pyrophosphatases
pyrophosphatases
bacterial inorganic
Prior art date
Application number
PCT/CA2003/001704
Other languages
French (fr)
Other versions
WO2004041856A2 (en
Inventor
Aled Edwards
Akil Dharamsi
Masoud Vedadi
Cristina Virag
Matthew Tai
Simon Houston
Robert Lam
Original Assignee
Affinium Pharm Inc
Aled Edwards
Akil Dharamsi
Masoud Vedadi
Cristina Virag
Matthew Tai
Simon Houston
Robert Lam
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affinium Pharm Inc, Aled Edwards, Akil Dharamsi, Masoud Vedadi, Cristina Virag, Matthew Tai, Simon Houston, Robert Lam filed Critical Affinium Pharm Inc
Priority to AU2003280261A priority Critical patent/AU2003280261A1/en
Publication of WO2004041856A2 publication Critical patent/WO2004041856A2/en
Publication of WO2004041856A3 publication Critical patent/WO2004041856A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to novel drug targets for pathogenic bacteria. Accordingly, the invention provides purified protein comprising the amino acid sequence set forth in SEQ ID NO: 4. The invention also provides biochemical and biophysical characteristics of the polypeptides of the invention.
PCT/CA2003/001704 2002-11-08 2003-11-07 Crystal structures of bacterial inorganic pyrophosphatases WO2004041856A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003280261A AU2003280261A1 (en) 2002-11-08 2003-11-07 Crystal structures of bacterial inorganic pyrophosphatases

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US42500902P 2002-11-08 2002-11-08
US60/425,009 2002-11-08
US43745802P 2002-12-31 2002-12-31
US60/437,458 2002-12-31
US43769103P 2003-01-02 2003-01-02
US60/437,691 2003-01-02

Publications (2)

Publication Number Publication Date
WO2004041856A2 WO2004041856A2 (en) 2004-05-21
WO2004041856A3 true WO2004041856A3 (en) 2004-08-26

Family

ID=32314886

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2003/001704 WO2004041856A2 (en) 2002-11-08 2003-11-07 Crystal structures of bacterial inorganic pyrophosphatases

Country Status (2)

Country Link
AU (1) AU2003280261A1 (en)
WO (1) WO2004041856A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113481180A (en) * 2021-07-05 2021-10-08 吉林大学 Alkaline thermophilic inorganic pyrophosphatase and application thereof in enhancing polymerase chain reaction and UDP-galactose synthesis reaction

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
AVAEVA SVETLANA ET AL: "Crystal structure of Escherichia coli inorganic pyrophosphatase complexed with SO-4-2-: Ligand-induced molecular asymmetry", FEBS LETTERS, vol. 410, no. 2-3, 1997, pages 502 - 508, XP002287033, ISSN: 0014-5793 *
DATABASE EMBL 1 September 2000 (2000-09-01), STOVER C K ET AL.: "Pseudomonas aeruginosa PAO1, section 381 of 529 of the complete genome", XP002287037, Database accession no. AE004820 *
HARUTYUNYAN EMIL H ET AL: "Crystal structure of holo inorganic pyrophosphatase from Escherichia coli at 1.9 A resolution: Mechanism of hydrolysis", BIOCHEMISTRY, vol. 36, no. 25, 1997, pages 7754 - 7760, XP002287031, ISSN: 0006-2960 *
HEIKINHEIMO PIRKKO ET AL: "The structural basis for pyrophosphatase catalysis", STRUCTURE (LONDON), vol. 4, no. 12, 1996, pages 1491 - 1508, XP002287098, ISSN: 0969-2126 *
KANKARE JUSSI ET AL: "Crystallographic identification of metal-binding sites in Escherichia coli inorganic pyrophosphatase", BIOCHEMISTRY, vol. 35, no. 15, 1996, pages 4670 - 4677, XP002287032, ISSN: 0006-2960 *
KANKARE JUSSI ET AL: "Structure of Escherichia coli inorganic pyrophosphatase at 2.2A resolution", ACTA CRYSTALLOGRAPHICA SECTION D BIOLOGICAL CRYSTALLOGRAPHY, vol. 52, no. 3, 1996, pages 551 - 563, XP008032493, ISSN: 0907-4449 *
LAHTI R ET AL: "CLONING AND CHARACTERIZATION OF THE GENE ENCODING INORGANIC PYROPHOSPHATASE OF ESCHERICHIA-COLI K-12", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 170, no. 12, December 1988 (1988-12-01), pages 5901 - 5907, XP002178879, ISSN: 0021-9193 *
LEPPANEN VELI-MATTI ET AL: "Sulfolobus acidocaldarius inorganic pyrophosphatase: Structure, thermostability, and effect of metal ion in an archael pyrophosphatase", PROTEIN SCIENCE, vol. 8, no. 6, June 1999 (1999-06-01), pages 1218 - 1231, XP002287035, ISSN: 0961-8368 *
OGANESSYAN V YU ET AL: "X-ray crystallographic studies of recombinant inorganic pyrophosphatase from Escherichia coli", FEBS (FEDERATION OF EUROPEAN BIOCHEMICAL SOCIETIES) LETTERS, vol. 348, no. 3, 1994, pages 301 - 304, XP002287040, ISSN: 0014-5793 *
STOVER C K ET AL: "COMPLETE GENOME SEQUENCE OF PSEUDOMONAS AERUGINOSA PA01, AN OPPORTUNISTIC PATHOGEN", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 406, no. 6799, 31 August 2000 (2000-08-31), pages 959 - 964, XP000996980, ISSN: 0028-0836 *
TEPLYAKOV A ET AL: "CRYSTAL STRUCTURE OF INORGANIC PYROPHOSPHATASE FROM Thermus thermophilus", PROTEIN SCIENCE, CAMBRIDGE UNIVERSITY PRESS, CAMBRIDGE, GB, vol. 3, no. 3, 1994, pages 1098 - 1107, XP002106478, ISSN: 0961-8368 *

Also Published As

Publication number Publication date
WO2004041856A2 (en) 2004-05-21
AU2003280261A1 (en) 2004-06-07
AU2003280261A8 (en) 2004-06-07

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