WO2004042043A3 - Crystal structures of bacterial ribulose-phosphate 3-epimerases - Google Patents

Crystal structures of bacterial ribulose-phosphate 3-epimerases Download PDF

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Publication number
WO2004042043A3
WO2004042043A3 PCT/CA2003/001672 CA0301672W WO2004042043A3 WO 2004042043 A3 WO2004042043 A3 WO 2004042043A3 CA 0301672 W CA0301672 W CA 0301672W WO 2004042043 A3 WO2004042043 A3 WO 2004042043A3
Authority
WO
WIPO (PCT)
Prior art keywords
epimerases
ribulose
bacterial
phosphate
crystal structures
Prior art date
Application number
PCT/CA2003/001672
Other languages
French (fr)
Other versions
WO2004042043A2 (en
Inventor
Aled Edwards
Akil Dharamsi
Masoud Vedadi
Megan Domagala
Benjamin Pinder
Muhammad Zahoor Alam
Kathleen Nethery
Simon Houston
Matthew Kimber
Teresa Clarke
Original Assignee
Affinium Pharm Inc
Aled Edwards
Akil Dharamsi
Masoud Vedadi
Megan Domagala
Benjamin Pinder
Muhammad Zahoor Alam
Kathleen Nethery
Simon Houston
Matthew Kimber
Teresa Clarke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Affinium Pharm Inc, Aled Edwards, Akil Dharamsi, Masoud Vedadi, Megan Domagala, Benjamin Pinder, Muhammad Zahoor Alam, Kathleen Nethery, Simon Houston, Matthew Kimber, Teresa Clarke filed Critical Affinium Pharm Inc
Priority to AU2003280248A priority Critical patent/AU2003280248A1/en
Publication of WO2004042043A2 publication Critical patent/WO2004042043A2/en
Publication of WO2004042043A3 publication Critical patent/WO2004042043A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Metallurgy (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to novel drug targets for pathogenic bacteria. Accordingly, the invention provides purified protein comprising the amino acid sequence set forth in SEQ ID NO: 4. The invention also provides biochemical and biophysical characteristics of the polypeptides of the invention.
PCT/CA2003/001672 2002-11-05 2003-11-05 Crystal structures of bacterial ribulose-phosphate 3-epimerases WO2004042043A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003280248A AU2003280248A1 (en) 2002-11-05 2003-11-05 Crystal structures of bacterial ribulose-phosphate 3-epimerases

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US42390602P 2002-11-05 2002-11-05
US60/423,906 2002-11-05
US43291702P 2002-12-12 2002-12-12
US60/432,917 2002-12-12

Publications (2)

Publication Number Publication Date
WO2004042043A2 WO2004042043A2 (en) 2004-05-21
WO2004042043A3 true WO2004042043A3 (en) 2004-12-23

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2003/001672 WO2004042043A2 (en) 2002-11-05 2003-11-05 Crystal structures of bacterial ribulose-phosphate 3-epimerases

Country Status (2)

Country Link
AU (1) AU2003280248A1 (en)
WO (1) WO2004042043A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008144012A2 (en) * 2007-05-14 2008-11-27 The Regents Of The University Of California Xylose- and arabinose- metabolizing enzyme -encoding nucleotide sequences with refined translational kinetics and methods of making same
WO2008153676A2 (en) * 2007-05-21 2008-12-18 The Regents Of The University Of California Pentose phosphate pathway and fermentation enzyme-encoding nucleotide sequences with refined translational kinetics and methods of making same
KR102080886B1 (en) * 2019-05-21 2020-02-24 씨제이제일제당 주식회사 ribulose-phosphate 3-epimerases comprising motifs having lower side reactions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998050555A2 (en) * 1997-05-06 1998-11-12 Human Genome Sciences, Inc. Enterococcus faecalis polynucleotides and polypeptides
WO2002034876A2 (en) * 2000-09-27 2002-05-02 Affinium Pharmaceuticals, Inc. Protein data analysis
US6617156B1 (en) * 1997-08-15 2003-09-09 Lynn A. Doucette-Stamm Nucleic acid and amino acid sequences relating to Enterococcus faecalis for diagnostics and therapeutics

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998050555A2 (en) * 1997-05-06 1998-11-12 Human Genome Sciences, Inc. Enterococcus faecalis polynucleotides and polypeptides
US6617156B1 (en) * 1997-08-15 2003-09-09 Lynn A. Doucette-Stamm Nucleic acid and amino acid sequences relating to Enterococcus faecalis for diagnostics and therapeutics
WO2002034876A2 (en) * 2000-09-27 2002-05-02 Affinium Pharmaceuticals, Inc. Protein data analysis

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
CHRISTENDAT D ET AL: "STRUCTURAL PROTEOMICS: PROSPECTS FOR HIGH THROUGHPUT SAMPLE PREPARATION", PROGRESS IN BIOPHYSICS AND MOLECULAR BIOLOGY, PERGAMON PRESS, OXFORD, GB, vol. 73, no. 5, 2000, pages 339 - 345, XP001154100, ISSN: 0079-6107 *
DATABASE Geneseq [online] 19 March 1999 (1999-03-19), "Enterococcus faecalis genome contig SEQ ID NO:229.", XP002302605, retrieved from EBI accession no. GSN:AAX13166 Database accession no. AAX13166 *
DATABASE Geneseq [online] 22 April 2004 (2004-04-22), "Enterococcus faecalis polypeptide #1299.", retrieved from EBI accession no. GSN:ADH86819 Database accession no. ADH86819 *
DATABASE UniProt [online] 1 June 2002 (2002-06-01), "Ribulose-phosphate 3-epimerase (EC 5.1.3.1).", XP002302606, retrieved from EBI accession no. UNIPROT:Q8RFL5 Database accession no. Q8RFL5 *
DATABASE UniProt [online] 1 June 2003 (2003-06-01), "Ribulose-phosphate 3-epimerase.", XP002302607, retrieved from EBI accession no. UNIPROT:Q82ZE2 Database accession no. Q82ZE2 *
DATABASE USPTO Proteins [online] 18 December 2003 (2003-12-18), "Sequence 4704 from patent US 6617156.", retrieved from EBI accession no. USPOP:AAR58170 Database accession no. AAR58170 *
EDWARDS A M ET AL: "PROTEIN PRODUCTION: FEEDING THE CRYSTALLOGRAPHERS AND NMR SPECTROSCOPISTS", NATURE STRUCTURAL BIOLOGY, NEW YORK, NY, US, vol. 7, no. SUPPL, November 2000 (2000-11-01), pages 970 - 972, XP001154099, ISSN: 1072-8368 *
KOPP, JURGEN ET AL: "Structure and Mechanism of the Amphibolic Enzyme D-Ribulose-5-phosphate 3-Epimerase from Potato Chloroplasts", JOURNAL OF MOLECULAR BIOLOGY , 287(4), 761-771 CODEN: JMOBAK; ISSN: 0022-2836, 1999, XP004455933 *
LESLEY SCOTT A ET AL: "Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 99, no. 18, 3 September 2002 (2002-09-03), pages 11664 - 11669, XP002302603, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
WO2004042043A2 (en) 2004-05-21
AU2003280248A8 (en) 2004-06-07
AU2003280248A1 (en) 2004-06-07

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