CN113481180A - Alkaline thermophilic inorganic pyrophosphatase and application thereof in enhancing polymerase chain reaction and UDP-galactose synthesis reaction - Google Patents

Alkaline thermophilic inorganic pyrophosphatase and application thereof in enhancing polymerase chain reaction and UDP-galactose synthesis reaction Download PDF

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CN113481180A
CN113481180A CN202110765804.0A CN202110765804A CN113481180A CN 113481180 A CN113481180 A CN 113481180A CN 202110765804 A CN202110765804 A CN 202110765804A CN 113481180 A CN113481180 A CN 113481180A
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高仁钧
李雅静
杨雪
解桂秋
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Xinya Lihua Biotechnology (Shanghai) Co.,Ltd.
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Abstract

An alkaline thermophilic Inorganic pyrophosphatase (Inorganic pyrophosphatase, PPase, EC3.6.1.1) and its application in the Reaction of enhancing Polymerase Chain Reaction (PCR) and synthesizing UDP-galactose belong to the technical field of biological engineering. The alkaline thermophilic inorganic pyrophosphatase is obtained by screening thermophilic bacteria Thermococcus ornurineus NA1, is marked as alkaline thermophilic PPase Ton1914, and the nucleotide sequence of the alkaline thermophilic inorganic pyrophosphatase is shown as SEQ ID No. 1. The result shows that the alkaline thermophilic PPase Ton1914 has obvious promotion effect on the amplification efficiency of DNA fragments with different lengths, and has more obvious amplification effect on DNA templates with small gene length. Alkaline thermophilic PPase Ton1914 is added into UDP-galactose generated by the reaction of 1-P-galactose catalyzed by UDP-glucose pyrophosphorylase TTE0732 and UTP, so that the reaction conversion rate can be further improved.

Description

Alkaline thermophilic inorganic pyrophosphatase and application thereof in enhancing polymerase chain reaction and UDP-galactose synthesis reaction
Technical Field
The invention belongs to the technical field of biological engineering, and particularly relates to alkaline thermophilic Inorganic pyrophosphatase (Inorganic pyrophosphatase, PPase, EC3.6.1.1) and application thereof in Polymerase Chain Reaction (PCR) Reaction enhancement and UDP-galactose synthesis.
Background
The product inhibition effect refers to a phenomenon that the terminal or by-product can reduce the enzymatic activity of the catalytic key step, and is one of the important factors which currently cause the reduction of the reaction yield of the natural enzyme, and has adverse effects on industrial production. In a living body, various vital activities such as DNA and RNA polymerization, amino acid activation, fatty acid oxidation, and synthesis of coenzyme, cellulose, starch require phosphorylation reaction to maintain a balance, and the phosphorylation reaction inevitably produces a by-Product Pyrophosphate (PPi) so that the phosphorylation reaction efficiency is suppressed. For example, in PCR, the reaction process generates a large amount of PPi to reduce the reaction efficiency, and thus, the elimination of PPi as a by-product can drive the forward movement of the phosphorylation reaction.
PCR is a molecular biology technique that can amplify a large number of DNA fragments using a small amount of DNA fragments. Due to the characteristics of high sensitivity, strong specificity, low purity requirement, simplicity and rapidness, the method plays a vital role in aspects of life research, clinical medicine, criminal investigation and the like. PCR generates a large amount of PPi as a by-product, thereby preventing forward progress of PCR, and thus, elimination of PPi is essential to improve PCR amplification efficiency. Since the reaction condition of PCR is high temperature and alkaline environment, the chemical method for removing PPi under acidic condition has poor effect. PPase is generally used for hydrolyzing PPi biologically, but the high temperature reaction environment of PCR can greatly reduce or even inactivate the activity of enzyme. Therefore, the development of new enzymes or other PCR enhancers that are resistant to high temperatures and alkali has been a hot spot. For example, patent CN104862288A uses a polymer-protein coupling technology to modify an N-isopropylacrylamide (PNIPAM) near the active center of a protein, the polymer has temperature responsiveness, can enhance the thermal stability of the protein to make the normal temperature protein adapt to the high temperature PCR condition, and simultaneously hydrolyzes PPi to achieve the effect of enhancing PCR, the final result shows that the thermal stability of the method at 60 ℃ is increased by 88% compared with the wild type, and the PCR efficiency is also significantly increased. Development of thermophilic PPase has been little achieved in recent years, for example, CN106834249A patent improves expression efficiency and thermostability of thermophilic PPase through mutation. However, no patent on the improvement of PCR amplification efficiency by thermophilic PPase has been reported. There are also commercially available thermophilic ppases, such as PPase extracted from e.g. the species hepacius stearothermophilus, but it is expensive and has limited purchasing channels. Therefore, the development of a highly efficient thermostable PPase is urgently needed. The invention clones and purifies thermophilic PPase from reduced sulfur hyperthermophilus, Thermococcus ornurineus NA1, and applies the thermophilic PPase to remove the byproduct PPi in PCR of DNA templates with different lengths, thereby effectively improving the PCR amplification efficiency. At present, no patent for improving PCR amplification efficiency by thermophilic PPase is found.
Disclosure of Invention
The invention aims to provide alkaline thermophilic Inorganic pyrophosphatase (Inorganic pyrophosphatase, PPase, EC3.6.1.1) and application thereof in Polymerase Chain Reaction (PCR) enhancement and UDP-galactose synthesis. The alkaline thermophilic Inorganic pyrophosphatase (Inorganic pyrophosphatase, PPase, EC3.6.1.1) is obtained by screening thermophilic bacteria Thermococcus onnenuineus NA1 in the laboratory, is marked as alkaline thermophilic PPase Ton1914, and the nucleotide sequence of the alkaline thermophilic Inorganic pyrophosphatase is shown as SEQ ID No. 1.
The alkaline thermophilic PPase Ton1914 is prepared by the following steps:
(1) obtaining an alkaline thermophilic PPase Ton1914 gene sequence (537bp, code 178aa, SEQ ID No.1) from thermophilic bacteria Thermococcus orniferus NA1 by NCBI GenBank search;
(2) designing a primer according to the nucleotide sequence SEQ ID No.1 of the enzyme, and amplifying the enzyme gene by utilizing PCR;
(3) connecting the recombinant plasmid with an escherichia coli expression vector pET28a to construct a recombinant plasmid, and transforming escherichia coli expression host bacteria BL21(DE3) competent cells to construct recombinase expression bacteria;
(4) the constructed recombinant enzyme expression bacteria are induced by an inducer IPTG (isopropyl thiogalactoside) to express the thermophilic inorganic pyrophosphatase. The expression product is soluble protein in cells, and the alkaline thermophilic PPase Ton1914 is obtained by separating and purifying through cell ultrasonic disruption and heating inactivation.
The second technical scheme of the invention is as follows: the optimum temperature, optimum pH, thermal stability and pH stability of the alkaline thermophilic PPase Ton1914 catalytic reaction are measured by the following specific operations:
(1) the optimal reaction temperature of the alkaline thermophilic PPase Ton1914 is explored by measuring the rate of recombinase catalyzing substrate hydrolysis within the range of 20-90 ℃, and the optimal pH of the alkaline thermophilic PPase Ton1914 is measured within the pH range of 6.0-11.0; the activity assay is described in example 2.
(2) Incubating alkaline thermophilic PPase Ton1914 at 70 ℃, 80 ℃ and 90 ℃ for different times, sampling, determining the residual activity of the alkaline thermophilic PPase Ton1914, and exploring the thermal stability of the alkaline thermophilic PPase Ton 1914; the activity assay is described in example 2.
(3) Respectively incubating alkaline thermophilic PPase Ton1914 in a buffer solution with the pH value of 6-11 for 1h and 24h, sampling and detecting the residual enzyme activity, and exploring the pH stability of the Ton 1914. The activity assay is described in example 2.
The invention provides a strategy for enhancing PCR amplification efficiency by detecting the enhancement effect of thermophilic alkaline inorganic pyrophosphatase on DNA fragments with the amplification product length of 500-6700 bp by utilizing Real-time fluorescence Quantitative (qPCR). The addition of alkaline thermophilic inorganic pyrophosphatase in the reaction for synthesizing UDP-galactose can improve the conversion rate of the reaction and provides a strategy for improving the yield of UDP-galactose.
The third technical scheme of the invention is as follows: the application of the alkaline thermophilic PPase Ton1914 in enhancing the PCR amplification efficiency is to add the alkaline thermophilic PPase Ton1914 into a PCR system to improve the reaction efficiency. The method comprises the following steps:
(1) the concentrations of 7 plasmid templates for amplifying fragments with different lengths, which are stored in a laboratory and can normally express proteins, are determined, and comprise pET28 a-Ttu 2902 (the length of an amplified fragment is 516bp), pET28a-GS01315 (the length of the amplified fragment is 954bp), pET28a-Aaci0783 (the length of the amplified fragment is 1215bp), pET28a-ST0779 (the length of the amplified fragment is 1756bp), pET28a-TL08779 (the length of the amplified fragment is 2494bp), pET28a-2552 (the length of the amplified fragment is 3162bp) and pET28 a-0602C 55S (the length of the amplified fragment is 6710 bp). Plasmid templates were obtained as exemplified by pET28a-Tfu 2902: obtaining a Ttu 2902 gene sequence through NCBI GenBank search, designing a primer according to the Ttu 2902 gene nucleotide sequence, and amplifying the gene by using PCR; the recombinant plasmid is connected with an escherichia coli expression vector pET28a after restriction enzyme digestion to construct a recombinant plasmid, namely a plasmid template pET28 a-Ttu 2902. The other six plasmid templates were obtained in a similar manner. The template concentration is 0.2-0.3 ng/. mu.L, the primer concentration is 300-500 nM, the PCR amplification efficiency is better, and finally the subsequent experiment is carried out by using the template concentration of 0.25 ng/. mu.L and the primer concentration of 300 nM.
(2) Optimizing the adding amount of alkaline thermophilic PPase Ton1914 in a PCR system: in the PCR, enzyme gradient addition (final concentration): 0 ng/mL-1.5 ng/mL. PCR amplification was performed by adding diluted Ton1914 to pET28a-Tfu2902 available in the laboratory as a template. The result confirms that the enzyme amount of 0.5-1.1 ng/mL has better promotion effect on PCR amplification efficiency, and finally 0.7ng/mL is selected to be added into a subsequent reaction system.
(3) Promotion of PCR efficiency for amplification of different fragment lengths by alkaline thermophilic PPase Ton 1914: qPCR was performed using 7 recombinant plasmids of different lengths obtained in step (1) as templates, and the effect of alkaline thermophilic PPase Ton1914 on the enhancement of PCR efficiency for amplifying different fragment lengths was investigated.
The fourth technical scheme of the invention is as follows: the use of alkaline thermophilic PPase Ton1914 in reactions for the synthesis of UDP-galactose.
UDP-sugar pyrophosphorylase TTE0732 can catalyze the reaction of 1-P-galactose (1-P-Gal) and UTP to produce UDP-galactose (UDP-Gal) (patent application No. 202110560931.7): 1-P-Gal + UTP → UDP-Gal + PPi. The reaction conversion rate can be further improved by adding alkaline thermophilic PPase Ton1914 to the reaction.
Drawings
FIG. 1: the optimum reaction temperature curve of alkaline thermophilic PPase Ton 1914; the abscissa is temperature and the ordinate is relative enzyme activity. The enzyme activity is rapidly improved at the temperature of more than 60 ℃, the relative activity of the enzyme can reach more than 95% at 70-80 ℃, the maximum enzyme activity is reached at 80 ℃, and 50% of the maximum enzyme activity can be reached at 80-90 ℃. The alkaline thermophilic PPase Ton1914 in the invention has catalytic activity at high temperature and can be used for removing PPi in PCR reaction.
FIG. 2: the optimum reaction pH curve of alkaline thermophilic PPase Ton 1914; the abscissa is pH value and the ordinate is relative enzyme activity. The enzyme activity is rapidly improved when the pH value reaches more than 8.0, the maximum activity is reached when the pH value reaches 9.0, and the enzyme activity can reach more than 40 percent of the maximum activity within the range of pH 8.0-9.5. It is demonstrated that alkaline thermophilic PPase Ton1914 can maintain higher activity under the PCR reaction condition.
FIG. 3: thermal stability curve of alkaline thermophilic PPase Ton 1914; the inset is the thermal stability curve of base Ton1914 at 70 ℃, with time on the abscissa and relative enzyme activity on the ordinate. The half-life period at 70 ℃ was 52h, and at 80 ℃ and 90 ℃ was 7h and 2.5h, respectively. It was demonstrated that alkaline thermophilic PPase Ton1914 is fully resistant to the high temperature denaturation step of the PCR reaction and remains highly active throughout the PCR reaction.
FIG. 4: pH stability curve of alkaline thermophilic PPase Ton 1914; the abscissa is pH value and the ordinate is relative enzyme activity. Wherein the dotted line is the pH stability curve for 1h and the solid line is the pH stability curve for 24 h. After incubation for 24h in the pH range of 7.0-10.0, the enzyme activity keeps more than 80% of the original activity, which indicates that the alkaline thermophilic PPase Ton1914 has high pH stability.
Fig. 5 to 11: an enhancement effect diagram of the alkaline thermophilic PPase Ton1914 on the PCR amplification efficiency of the amplified fragment with the length of 516-6710 bp; the templates used in fig. 5 to 11 are: pET28 a-Ttu 2902 (length of amplified fragment is 516bp), pET28a-GS01315 (length of amplified fragment is 954bp), pET28a-Aaci0783 (length of amplified fragment is 1215bp), pET28a-ST0779 (length of amplified fragment is 1756bp), pET28a-TL08779 (length of amplified fragment is 2494bp), pET28a-2552 (length of amplified fragment is 3162bp), pET28a-Tn0602C55S (length of amplified fragment is 6710 bp). a is a qPCR fluorescence spectrum showing the amount of product produced with increasing number of qPCR cycles, with the abscissa being the number of PCR amplification cycles and the ordinate being the change in fluorescence intensity; and b is an agarose gel electrophoresis image after qPCR is finished, wherein 1 and 2 are respectively the reaction system without adding alkaline thermophilic PPase Ton1914 and with adding alkaline thermophilic PPase Ton1914, and M is a nucleic acid marker. The result shows that the alkaline thermophilic PPase Ton1914 has obvious promotion effect on the amplification efficiency of DNA fragments with different lengths, and the product quantity is obviously higher than that of the group without the alkaline thermophilic PPase Ton 1914.
Detailed Description
Example 1: the construction of alkaline thermophilic PPase Ton1914 engineering bacteria and the expression and purification of protein comprise the following steps:
cloning of alkaline thermophilic PPase Ton1914 Gene
The alkaline thermophilic PPase Ton1914 primer sequence is as follows:
an upstream primer: 5' AGCATGCCATGGTGAACCCGTTCCAC 3'
A downstream primer: 5' GGACCGCTCGAGCTCCTTCTTACCGAAC 3'
The upstream endonuclease site is Nco I (underlined), and the downstream endonuclease site is Xho I (underlined);
table 1: PCR system
Reagent Volume (μ L)
Distilled Water (DW) 68
5×Buffer 20
dNTPs 8
Upstream primer 1
Downstream primer 1
Genomic DNA (laboratory preservation) 1
pfu 1
PCR conditions were as follows: 94 ℃ for 3 min; 94 ℃, 30s, 60 ℃, 30s, 72 ℃, 1min, 30 cycles; 72 ℃ for 10 min; and preserving at 4 ℃.
Expression purification of alkaline thermophilic PPase Ton1914
The alkaline thermophilic PPase Ton1914 PCR product is cut by restriction enzymes Nco I and Xho I, 5 mu L of 10 XBuffer, 25 mu L of PCR product, 1.5 mu L of each restriction enzyme and 15 mu L of distilled water are added in sequence in the cutting system, and the mixture is cut by enzyme for 6h at 37 ℃ after being mixed evenly. mu.L of alkaline thermophilic PPase Ton1914 enzyme digestion product and 3. mu.L of pET28a carrier fragment, 1. mu.L of 10 XT4DNA buffer and 1. mu. L T4The DNA Ligase was mixed and ligated overnight at 16 ℃. mu.L of the ligation mixture was used to transform 100. mu.L of E.coli competent cell DH 5. alpha. and recombinant plasmids were selected. Transforming the recombinant plasmid into Escherichia coli competent cell BL21(DE3), and culturing to OD600When the concentration is 0.6-1.0, 0.5mM IPTG is added to the mixture to carry out shaking culture at 25 ℃ overnight, and the expression of the target protein is induced. The expressed protein is subjected to cell disruption, centrifugation at 12000rpm for 15min, and heat treatment at 80 ℃ for 10min to obtain the alkaline thermophilic PPase Ton1914 pure protein.
The methods of plasmid extraction, E.coli competent cell preparation and vector transformation were referred to "molecular cloning protocols" (third edition, scientific Press, 2002).
Example 2: determination of optimum temperature, optimum pH, thermal stability and pH stability of alkaline thermophilic PPase Ton1914
1. Determination of optimum reaction temperature
The optimal reaction temperature of alkaline thermophilic PPase Ton1914 is explored by measuring the rate of substrate hydrolysis catalyzed by recombinase at 20-90 ℃. To the reaction system were added 4mL of 50mM Tris-HCl (pH 9.0), 1mL of 10mM sodium pyrophosphate, and 10mM MgCl in this order21mL and alkaline thermophilic PPase Ton19140.1mL, mixing uniformly, reacting at different temperatures for 10 minutes, placing on ice to stop reaction, and taking 1mL of reaction solution for activity determination. The activity determination method comprises the following steps: formation of phosphomolybdic acid by reaction of orthophosphate with ammonium molybdate, FeSO under mild acid conditions4The phosphate, the hydrolysis product of PPi, was detected on the principle of reduction to a blue compound and an absorption peak at 660 mm. 5mL of ammonium molybdate-FeSO was added to the reaction system4The color developing agent is a color developing substrate, and 1mL of the reaction solution and deionized water are added to make up a 10mL system. The control group did not contain alkaline thermophilic PPase Ton1914, and the other components were identical to those of the experimental group. After incubation at room temperature for 10min, the reacted experimental group and control group were transferred to a 1mL cuvette and absorbance at 660nm was measured in a spectrophotometer.
2. Determination of optimum reaction pH
The optimum reaction pH of alkaline thermophilic PPase Ton1914 was measured in the range of pH 6.0-11.0, and the activity was measured at 70 ℃ in 50mM Tris-HCl buffer (pH6.0, 7.0, 8.0 and 9.0) and 50mM Gly-NaOH buffer (pH 9.0, 10.0 and 11.0). The system and method for determining viability are the same as above.
3. Determination of thermal stability
The alkaline thermophilic PPase Ton1914 is incubated at 70 ℃, 80 ℃ and 90 ℃, samples are taken at intervals of 0.5h, 2h and 4h respectively, and the thermal stability of the enzyme within 100h is detected. The system and method for determining viability are the same as above.
Determination of pH stability
And (3) measuring the pH stability of the alkaline thermophilic PPase Ton1914 within the range of pH 6.0-11.0, wherein the incubation time is 1h and 24h, and sampling to detect the residual enzyme activity. The system and method for determining viability are the same as above.
Example 3: application of alkaline thermophilic PPase Ton1914 in enhancing PCR efficiency
Alkaline thermophilic PPase Ton1914 with the final concentration of 0 ng/mL-1.5 ng/mL was added to the system of Table 2 using pET28a-Tfu2902 recombinant plasmid as a template, and qPCR was performed to determine the amount of alkaline thermophilic PPase Ton1914 added.
Table 2: qPCR reaction system dosage scale
Reagent Volume (μ L)
Premixed liquid of kit 10
Upstream primer 2
Downstream primer 2
Sterile Water/Ton 1914 2
Form panel 4
Adding 10 mu L of the premixed solution of the kit and 4 mu L of the plasmid template pET28a-Tfu2902 into a PCR tube, sequentially adding 2 mu L of each of the upstream primer and the downstream primer, finally adding 2 mu L of alkaline thermophilic PPase Ton1914 with different concentrations, and uniformly mixing to perform qPCR. Reaction procedure for qPCR: at 50 ℃ for 2 min; pre-denaturation at 95 ℃ for 2 min; denaturation at 95 ℃ for 15s, annealing/extension at 60 ℃ for 1 min; return to denaturation and repeat for 30 cycles; and preserving at 4 ℃. After the amplification, the detection of the amplified product was performed by 0.8% agarose gel electrophoresis. Finally, the effect of promoting the PCR amplification efficiency by the addition amount of 0.5-1.1 ng/mL of alkaline thermophilic PPase Ton1914 is determined to be good, and the addition amount of 0.7ng/mL is selected for carrying out the later experiment.
Determination of the concentration of 7 plasmid templates: pET28a-Tfu2902, pET28a-GS01315, pET28a-Aaci0783, pET28a-ST0779, pET28a-TL08779, pET28a-2552 and pET28a-Tn0602C55S are selected as templates for PCR amplification, templates with different concentrations are added into a PCR system, and the PCR system is subjected to PowerUp detectionTM SYBRTMThe Green Master Mix kit is operated according to the instruction, the result shows that the template concentration is 0.2-0.3 ng/mu L, the primer concentration is 300-500 nM, the PCR amplification efficiency is better, and finally the subsequent experiment is carried out according to the template concentration of 0.25 ng/mu L and the primer concentration of 300 nM. When qPCR is carried out, 10 mu L of kit premix solution is firstly added, 4 mu L of template (the final concentration is 0.25 ng/mu L), 2 mu L of each of the upstream primer and the downstream primer is then sequentially added, and finally 2 mu L of alkaline thermophilic PPase Ton1914 (the final concentration is 0.7ng/mL) is added, qPCR is carried out after uniform mixing, and the promotion effect of the alkaline thermophilic PPase Ton1914 on the PCR efficiency of amplifying products with different lengths is researched through analyzing the product quantity by a fluorescence spectrum and agarose gel electrophoresis. The control group was not supplemented with alkaline thermophilic PPase Ton1914, and the other components were the same as those in the experimental group. The template was 7 recombinant plasmids described in 2, where pET28a-Tfu290 was used to amplify a product of 516bp in length, pET28a-GS01315 was used to amplify a product of 954bp in length, pET28a-Aaci0783 was used to amplify a product of 1215bp in length, pET28a-ST0779 was used to amplify a product of 1756bp in length, pET28a-TL08779 was used to amplify a product of 2494bp in length, pET28a-2522 was used to amplify a product of 3162bp in length, and pET28a-Tn0602C55S was used to amplify a product of 6710bp in length. The results show that the alkaline thermophilic PPase Ton1914 has the promotion effect on the reactions, and the product amount is obviously higher than that of the group without the alkaline thermophilic PPase Ton 1914.
Example 4: application of alkaline thermophilic PPase Ton1914 in reaction for synthesizing UDP-galactose
UDP-sugar pyrophosphorylase TTE0732 can catalyze the reaction of 1-P-galactose (1-P-Gal) and UTP to generate UDP-galactose (UDP-Gal): 1-P-Gal + UTP → UDP-Gal + PPi. Further, alkaline thermophilic PPase Ton1914 was added to the reaction to increase the reaction conversion. The composition data of the reaction system are shown in Table 2:
table 3: compositional data of the reaction System
Figure BDA0003147685110000071
Figure BDA0003147685110000081
Reaction conditions are as follows: incubating at 50 ℃ for 5h, terminating the reaction after the reaction is finished in a boiling water bath for 1min, centrifuging at 12000rpm for 3min, collecting supernatant, and passing through a 0.22 mu m water film for HPLC liquid phase detection of the residual UTP. The results of the experiment showed that the conversion rate of the reaction (conversion rate ═ UTP) was obtained after addition of alkaline thermophilic PPase Ton1914(blank group)-UTP(Experimental group))/UTP (blank group)100%) increased from 30% in experimental group 1 to 57% in experimental group 2.
The present disclosure is believed to provide those skilled in the art with a full range of applicability. The foregoing preferred embodiments are therefore to be understood as merely illustrative and not limiting of the scope of the invention in any way.
<110> Jilin university
<120> alkaline thermophilic inorganic pyrophosphatase and its use for enhancing polymerase chain reaction and synthesis of UDP-galactose
<160> 2
<170> PatentIn version 3.5
<210> 1
<211> 537
<212> DNA
<213> Thermococcus onnurineus NA1
<221> CDS
<222> (1)..(537)
<400> 1
atg aac ccg ttc cac gag ctt gag ccc gga ccg gag gtt cca gag gtc 48
Met Asn Pro Phe His Glu Leu Glu Pro Gly Pro Glu Val Pro Glu Val
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gtt tac gct ctt ata gag att ccg aag gga agc agg aac aag tac gag 96
Val Tyr Ala Leu Ile Glu Ile Pro Lys Gly Ser Arg Asn Lys Tyr Glu
20 25 30
ctc gac aag aag acc ggc ctt ctc aag ctt gat aga gtg ctc tac agc 144
Leu Asp Lys Lys Thr Gly Leu Leu Lys Leu Asp Arg Val Leu Tyr Ser
35 40 45
ccg ttc ttc tac ccg gtt gac tac gga atc atc ccg cag acc tgg tac 192
Pro Phe Phe Tyr Pro Val Asp Tyr Gly Ile Ile Pro Gln Thr Trp Tyr
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gac gac ggc gac ccc ttt gac ata atg gtc atc atg cgc gag ccg gtc 240
Asp Asp Gly Asp Pro Phe Asp Ile Met Val Ile Met Arg Glu Pro Val
65 70 75 80
tac ccg ctc acc atc gtc gag gcc agg ccg ata ggc atc atg aag atg 288
Tyr Pro Leu Thr Ile Val Glu Ala Arg Pro Ile Gly Ile Met Lys Met
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gag gac agc gga gac aaa gac tgg aag gtt ctc gcc gtt ccc gtc gag 336
Glu Asp Ser Gly Asp Lys Asp Trp Lys Val Leu Ala Val Pro Val Glu
100 105 110
gat ccg tac ttc aat gac tgg aag gac atc gac gac gtt ccg aag gcc 384
Asp Pro Tyr Phe Asn Asp Trp Lys Asp Ile Asp Asp Val Pro Lys Ala
115 120 125
ttc ctt gac gag ata gcc cac ttc ttc cag agg tac aag gag ctc cag 432
Phe Leu Asp Glu Ile Ala His Phe Phe Gln Arg Tyr Lys Glu Leu Gln
130 135 140
ggc aaa gtc acc acc gtc gaa ggc tgg ggc acc gcc gag gag gcc aag 480
Gly Lys Val Thr Thr Val Glu Gly Trp Gly Thr Ala Glu Glu Ala Lys
145 150 155 160
agg gaa atc ctc agg gcc atc gag atg tac aaa gag aag ttc ggt aag 528
Arg Glu Ile Leu Arg Ala Ile Glu Met Tyr Lys Glu Lys Phe Gly Lys
165 170 175
aag gag tga 537
Lys Glu

Claims (3)

1. An alkaline thermophilic PPase Ton1914, the nucleotide sequence of which is shown in SEQ ID No. 1.
2. Use of the alkaline thermophilic PPase Ton1914 according to claim 1 for enhancing the polymerase chain reaction.
3. Use of the alkaline thermophilic PPase Ton1914 according to claim 1 in reactions for the synthesis of UDP-galactose.
CN202110765804.0A 2021-07-05 2021-07-05 Alkaline thermophilic inorganic pyrophosphatase and application thereof in enhancing polymerase chain reaction and UDP-galactose synthesis reaction Pending CN113481180A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114507649A (en) * 2022-02-16 2022-05-17 吉林大学 Thermophilic enzyme and method for efficiently synthesizing UDP-glucose and UDP-glucuronic acid by one-pot method

Citations (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9208211D0 (en) * 1991-05-31 1992-05-27 Ciba Geigy Ag Improved process for the production of glycosyltransferases
US5264352A (en) * 1991-04-09 1993-11-23 Hoechst Aktiengesellschaft Process for the enzymatic synthesis of 2-deoxy-β-D-galactosides
WO1996012798A1 (en) * 1994-10-25 1996-05-02 New England Biolabs, Inc. Purified thermostable inorganic pyrophosphatase obtainable from thermococcus litoralis
WO1997037038A1 (en) * 1996-03-29 1997-10-09 Boehringer Mannheim Gmbh Process for the specific multiplication of long nucleic acids by pcr
US20020025560A1 (en) * 1996-09-17 2002-02-28 Satoshi Koizumi Processes for producing sugar nucleotides and complex carbohydrates
US20020034805A1 (en) * 1997-12-15 2002-03-21 Michel Gilbert Fusion proteins for use in enzymatic synthyesis of oligosaccharides
US20020132320A1 (en) * 2001-01-10 2002-09-19 Wang Peng George Glycoconjugate synthesis using a pathway-engineered organism
US20020150968A1 (en) * 2001-01-10 2002-10-17 Wang Peng G. Glycoconjugate and sugar nucleotide synthesis using solid supports
US20030049655A1 (en) * 2001-04-30 2003-03-13 Clark Duncan Roy Amplification process
JP2003189891A (en) * 1996-09-17 2003-07-08 Kyowa Hakko Kogyo Co Ltd Method for producing sugar nucleotide and complex carbohydrate
AU2003280261A8 (en) * 2002-11-08 2004-06-07 Affinium Pharm Inc Crystal structures of bacterial inorganic pyrophosphatases
CN1668739A (en) * 2002-07-15 2005-09-14 纳瓦拉公立大学 Plant nucleotide-sugar pyrophosphatase/phosphodiesterase (NPPase), method of obtaining same and use of same in the production of assay devices and in the production of transgenic plants
WO2007057167A2 (en) * 2005-11-18 2007-05-24 Bioline Limited A method for enhancing enzymatic dna polymerase reactions
KR20080047754A (en) * 2006-11-27 2008-05-30 (주)바이오니아 Thermostable pyrophosphatase from picrophilus torridus
US7901912B1 (en) * 2004-10-21 2011-03-08 Yamasa Corporation Method of producing uridine 5′-diphospho-N-acetylgalactosamine
CN102858965A (en) * 2010-04-30 2013-01-02 霍夫曼-拉罗奇有限公司 System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
US20150044683A1 (en) * 2012-03-09 2015-02-12 Bioneer Corporation Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction
CN104862288A (en) * 2015-05-08 2015-08-26 苏州大学 Synthesis of novel temperature-responsive inorganic pyrophosphatase conjugate and application of conjugate in enhanced polymerase chain reaction
CN105821017A (en) * 2016-03-31 2016-08-03 中国农业科学院作物科学研究所 Application of maize pyrophosphorylase gene ZmPPase 4 to improvement of plant stress resistance
WO2017070164A2 (en) * 2015-10-19 2017-04-27 University Of Florida Research Foundation, Incorporated A thermostable haloarchaeal inorganic pyrophosphatase
CN106834249A (en) * 2017-01-10 2017-06-13 广州海力特生物科技有限公司 A kind of thermally-stabilised inorganic pyrophosphatase of transformation
CN108998403A (en) * 2018-09-19 2018-12-14 长春大学 A kind of bacillus subtilis recombinant bacterium and its application
WO2021089251A1 (en) * 2019-11-05 2021-05-14 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Enzymatic method for preparation of udp-galactose
CN113265434A (en) * 2021-05-19 2021-08-17 吉林大学 Method for synthesizing UDP-galactose and galactosyl compound
CN114507649A (en) * 2022-02-16 2022-05-17 吉林大学 Thermophilic enzyme and method for efficiently synthesizing UDP-glucose and UDP-glucuronic acid by one-pot method
WO2023250198A1 (en) * 2022-06-24 2023-12-28 Zymtronix Catalytic Systems, Inc. Biocatalytic manufacture of sugar nucleotides

Patent Citations (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264352A (en) * 1991-04-09 1993-11-23 Hoechst Aktiengesellschaft Process for the enzymatic synthesis of 2-deoxy-β-D-galactosides
GB9208211D0 (en) * 1991-05-31 1992-05-27 Ciba Geigy Ag Improved process for the production of glycosyltransferases
WO1996012798A1 (en) * 1994-10-25 1996-05-02 New England Biolabs, Inc. Purified thermostable inorganic pyrophosphatase obtainable from thermococcus litoralis
US5861296A (en) * 1994-10-25 1999-01-19 New England Biolabs, Inc. Purified thermostable inorganic pyprophosphatase obtainable from thermococcus litoralis
WO1997037038A1 (en) * 1996-03-29 1997-10-09 Boehringer Mannheim Gmbh Process for the specific multiplication of long nucleic acids by pcr
JP2003189891A (en) * 1996-09-17 2003-07-08 Kyowa Hakko Kogyo Co Ltd Method for producing sugar nucleotide and complex carbohydrate
US20020025560A1 (en) * 1996-09-17 2002-02-28 Satoshi Koizumi Processes for producing sugar nucleotides and complex carbohydrates
CN1550554A (en) * 1996-09-17 2004-12-01 Э�ͷ��͹�ҵ��ʽ���� Processes for producing sugar nucleotides and complex carbohydrates
US20020034805A1 (en) * 1997-12-15 2002-03-21 Michel Gilbert Fusion proteins for use in enzymatic synthyesis of oligosaccharides
US20020150968A1 (en) * 2001-01-10 2002-10-17 Wang Peng G. Glycoconjugate and sugar nucleotide synthesis using solid supports
US20020132320A1 (en) * 2001-01-10 2002-09-19 Wang Peng George Glycoconjugate synthesis using a pathway-engineered organism
US20030049655A1 (en) * 2001-04-30 2003-03-13 Clark Duncan Roy Amplification process
CN1522305A (en) * 2001-04-30 2004-08-18 Amplification process
CN1668739A (en) * 2002-07-15 2005-09-14 纳瓦拉公立大学 Plant nucleotide-sugar pyrophosphatase/phosphodiesterase (NPPase), method of obtaining same and use of same in the production of assay devices and in the production of transgenic plants
AU2003280261A8 (en) * 2002-11-08 2004-06-07 Affinium Pharm Inc Crystal structures of bacterial inorganic pyrophosphatases
US7901912B1 (en) * 2004-10-21 2011-03-08 Yamasa Corporation Method of producing uridine 5′-diphospho-N-acetylgalactosamine
WO2007057167A2 (en) * 2005-11-18 2007-05-24 Bioline Limited A method for enhancing enzymatic dna polymerase reactions
KR20080047754A (en) * 2006-11-27 2008-05-30 (주)바이오니아 Thermostable pyrophosphatase from picrophilus torridus
CN102858965A (en) * 2010-04-30 2013-01-02 霍夫曼-拉罗奇有限公司 System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
US20150044683A1 (en) * 2012-03-09 2015-02-12 Bioneer Corporation Composition for hot-start reverse transcription reaction or hot-start reverse transcription polymerase chain reaction
CN104862288A (en) * 2015-05-08 2015-08-26 苏州大学 Synthesis of novel temperature-responsive inorganic pyrophosphatase conjugate and application of conjugate in enhanced polymerase chain reaction
WO2017070164A2 (en) * 2015-10-19 2017-04-27 University Of Florida Research Foundation, Incorporated A thermostable haloarchaeal inorganic pyrophosphatase
CN105821017A (en) * 2016-03-31 2016-08-03 中国农业科学院作物科学研究所 Application of maize pyrophosphorylase gene ZmPPase 4 to improvement of plant stress resistance
CN106834249A (en) * 2017-01-10 2017-06-13 广州海力特生物科技有限公司 A kind of thermally-stabilised inorganic pyrophosphatase of transformation
CN108998403A (en) * 2018-09-19 2018-12-14 长春大学 A kind of bacillus subtilis recombinant bacterium and its application
WO2021089251A1 (en) * 2019-11-05 2021-05-14 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Enzymatic method for preparation of udp-galactose
CN114641577A (en) * 2019-11-05 2022-06-17 马克斯·普朗克科学促进学会 Enzymatic process for the preparation of UDP-galactose
CN113265434A (en) * 2021-05-19 2021-08-17 吉林大学 Method for synthesizing UDP-galactose and galactosyl compound
CN114507649A (en) * 2022-02-16 2022-05-17 吉林大学 Thermophilic enzyme and method for efficiently synthesizing UDP-glucose and UDP-glucuronic acid by one-pot method
WO2023250198A1 (en) * 2022-06-24 2023-12-28 Zymtronix Catalytic Systems, Inc. Biocatalytic manufacture of sugar nucleotides

Non-Patent Citations (16)

* Cited by examiner, † Cited by third party
Title
JIANBO ZHANG等: "Large-scale Synthesis of Carbohydrates for Pharmaceutical Development", CURRENT ORGANIC CHEMISTRY, vol. 2000, 1 December 2001 (2001-12-01), pages 1169 - 1176 *
LEE, H.S.等: "Thermococcus onnurineus NA1, complete genome", GENBANK DATABASE, 31 January 2014 (2014-01-31), pages 000855 *
LEE,H.S.等: "Thermococcus onnurineus NA1, complete genome", 《GENBANK DATABASE》 *
LEE,H.S.等: "Thermococcus onnurineus NA1, complete genome", 《GENBANK DATABASE》, 31 January 2014 (2014-01-31), pages 000855 *
SUN YOUNG PARK等: "Facilitation of polymerase chain reaction with thermostable inorganic pyrophosphatase from hyperthermophilic archaeon Pyrococcus horikoshii", 《APPL MICROBIOL BIOTECHNOL》, vol. 85, 31 October 2009 (2009-10-31), pages 807 - 812, XP019778604 *
YANG ZOU等: "One-pot three-enzyme synthesis of UDP-Glc, UDP-Gal, and their derivatives", CARBOHYDRATE RESEARCH, 24 May 2013 (2013-05-24), pages 76 - 81 *
刘原子等: "腾冲嗜热厌氧杆菌tte0732 基因的表达及生物信息学分析", 微生物杂志, vol. 38, no. 1, 15 February 2018 (2018-02-15), pages 44 *
刘春红等: "平贝母多糖对D-半乳糖诱导衰老模型小鼠的抗氧化作用", 《食品科学》 *
刘春红等: "平贝母多糖对D-半乳糖诱导衰老模型小鼠的抗氧化作用", 《食品科学》, no. 23, 15 December 2011 (2011-12-15), pages 292 - 295 *
尹恒等: "茶尺蠖无机焦磷酸酶EoPPase672的表达与功能研究", 《昆虫学报》 *
尹恒等: "茶尺蠖无机焦磷酸酶EoPPase672的表达与功能研究", 《昆虫学报》, vol. 65, no. 4, 24 April 2022 (2022-04-24), pages 427 - 436 *
王迎华;曹郁生;陈燕;高丹丹;: "galU基因在乳酸乳球菌中的克隆及其对胞外多糖产生的影响", 天然产物研究与开发, no. 04, 15 August 2009 (2009-08-15), pages 1 - 6 *
王鸿利等主编: "《中华检验医学大辞典》", vol. 2000, 上海科学技术出版社, pages: 48 *
舍英著: "《应用同功酶学》", 31 December 2001, 内蒙古人民出版社, pages: 350 - 352 *
许韶华等: "糖基转移酶活性供体UDP-糖的生物合成及循环再生", 《药物生物技术》 *
许韶华等: "糖基转移酶活性供体UDP-糖的生物合成及循环再生", 《药物生物技术》, vol. 26, no. 3, 15 June 2019 (2019-06-15), pages 244 - 249 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114507649A (en) * 2022-02-16 2022-05-17 吉林大学 Thermophilic enzyme and method for efficiently synthesizing UDP-glucose and UDP-glucuronic acid by one-pot method
CN114507649B (en) * 2022-02-16 2023-11-21 吉林大学 Thermophilic enzyme and method for efficiently synthesizing UDP-glucose and UDP-glucuronic acid by one-pot method

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