WO2004038392A1 - 試料分析時の補正方法、分析装置および分析用具 - Google Patents
試料分析時の補正方法、分析装置および分析用具 Download PDFInfo
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- WO2004038392A1 WO2004038392A1 PCT/JP2003/013669 JP0313669W WO2004038392A1 WO 2004038392 A1 WO2004038392 A1 WO 2004038392A1 JP 0313669 W JP0313669 W JP 0313669W WO 2004038392 A1 WO2004038392 A1 WO 2004038392A1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
Definitions
- the present invention relates to a technique for correcting the influence of the characteristics of the reaction system on the analysis result at the time of analyzing a sample liquid when analyzing a sample using a reaction system in which a sample and a reagent are reacted.
- a method of analyzing a sample there is a method using an optical method.
- a reaction system is constructed by reacting a sample with a reagent in an analytical tool, while a reaction system is irradiated with light in an analyzer, and a response from the reaction system at that time (for example, see US Pat. No. 3,526,480).
- Adopting such an analytical method: ⁇ Analytical accuracy is known to be affected by the pigment components (eg, bilirubin (Bil) and hemoglobin (Hb)) contained in the sample or the lunar quality.
- the pigment components eg, bilirubin (Bil) and hemoglobin (Hb)
- the amount of light absorbed in the reaction system increases.
- the amount of Bil or Hb contained in a sample is not always the same between samples, and dye components such as Bil and Hb are oxidized with time during storage of the sample, resulting in absorption spectra. Change. Therefore, the amount of light absorbed in the reaction system varies depending on the amount of the dye component and the degree to which the dye component is oxidized. Also, the release of Ml and Hb from oxidation is affected by pH, and Bil and Hb tend to be more easily oxidized in an alkaline environment. Therefore, the amount of light absorbed in the reaction system differs depending on the reaction conditions in the reaction system, because the amount of Mt that Bil and Hb are oxidized differs depending on the pH when the sample is reacted with the reagent. It becomes.
- the lipid is dissolved in the reaction system by a nonionic (non-ionic) surfactant during the analysis, and the degree of turbidity (chylline) before and after the reaction is increased. Will be different. Therefore, contains surfactant In a system, the effect on the response before and after the reaction is different.
- Planck correction is performed by acquiring correction information using a measurement system including a sample and a blank reagent, and correcting the analysis result based on the correction information. For example, in order to consider the effect of the dye component, it is necessary to perform a blank measurement in a system that has a pH similar to that of the reaction system and contains a powerful sample. On the other hand, in order to consider the effects of lipids, it is necessary to perform a blank measurement in a system containing the same amount of surfactant and sample as the reaction system.
- urine tests for example, often involve testing multiple items from a single sample, but the types and factors that affect the analytical accuracy are often different. The @ that these factors affect differ for each analysis item. Therefore, when a plurality of items are analyzed from one type of sample, it is preferable to perform blank correction for each analysis item. '
- the number of required blank measurements is increased, and thus has the following problems. That is, first, the total amount of reagents required for plank correction increases, which is disadvantageous in cost. Second, since a sample is required for each blank measurement, the total amount of samples required for analysis increases, and the burden on the subject increases when analyzing biological samples such as urine and blood. . Third, in order to analyze multiple items with a single analysis tool, it is necessary to secure more reaction areas for blank measurement in the analysis tool. Alternatively, the space allocated to each reaction area becomes smaller. Disclosure of the invention
- An object of the present invention is to make it possible to carry out blank correction on ⁇ lj at a low cost based on a small number of samples without obstructing the small size of the analytical tool.
- a method of performing analysis on complex difficult analysis items based on a reaction solution obtained by reacting a reagent with a sample is described. This is a method for making corrections when performing analysis. For a plurality of similar analysis items, the same blanking measurement results are used to make adjustments, and the method of correction during sample analysis is shared.
- a plurality of suitable analytical items are analyzed at the time of analysis. Conditions similar to each other are grouped into similar groups and divided into a plurality of groups. In addition, multiple blank measurements with different measurement conditions were performed, and based on the analysis of the individual analysis items that make up each group described above, based on the results of the blank measurement corresponding to the group, Corrections are made.
- an analyzer for analyzing a plurality of specific components in a sample based on a reaction solution obtained by reacting the sample with a reagent comprising:
- the operation means may perform an analysis when performing an operation for analyzing the plurality of specific components.
- the present invention provides a loaf apparatus configured to perform correction based on correction information obtained based on the same plank measurement result for a plurality of specific components similar in reaction condition force S at the time.
- the analyzer be further provided with a correction means for obtaining the correction information based on the result of the blank measurement. ⁇
- an analysis tool comprising: a plurality of analysis reagent sections each containing a different reagent; and one or a plurality of blank measurement reagent sections.
- the plurality of Planck measurement reagent sections are common to the plurality of difficult-to-combine analysis reagent sections having similar reaction conditions at the time of analysis. You.
- Reaction conditions serving as standards for standardizing blank measurement include, for example, the attributes and composition of the reaction solution.
- the attribute of the reaction solution typically includes the pH of the reaction solution.
- the composition of the reaction solution typically includes a force containing a surfactant force S. Therefore, in the present invention, the analysis items having similar reaction conditions are, for example, those in which the pH of the reaction solution at the time of analysis is close to a certain level, and a certain level is the pH at the time of analysis.
- the reaction solution contains (but does not contain) a surfactant.
- a surfactant may be sharing the blank measurement system.
- the blank measurement in the present invention is performed in at least one measurement system selected from, for example, a raw blank measurement system, a neutral blank measurement system, an alkaline plank measurement system, and a surfactant blank measurement system including a surfactant.
- the acidic blank measurement system is set, for example, at a pH value selected from the range of 3.0 to 5.5, and is constructed with a carboxylic acid-based buffer solution such as Qian Kyaukhoshoku.
- the salt concentration of the buffer is, for example, 0.05 to 0.5 mol / L.
- the neutral blank measurement system is set, for example, at a pH of around 7.0, and is constructed with phosphorus and a buffer solution.
- the salt concentration of the buffer is, for example, 0.05 to 0.5 fflol / L.
- the pH value of the blank measurement system is set to a value selected from the range of 8.5 to 11.0, and a cyclohexylamino etasulfonic acid (CHES) buffer, cyclohexaminopropane is used. Built with sulfonic acid (CAPS) buffer.
- the salt concentration of the buffer is, for example, 0.05 to 0.5 mol / L. acid!
- the raw blank measurement system, neutral blank measurement system, and neutral blank measurement system may be constructed using a buffer containing a salt of a compound.
- the alkaline measurement system may be constructed using a good buffer (Good's buffer).
- the surfactant blank measurement system is constructed as a system capable of solubilizing lipids such as triceride neutral fat in a solution, for example, a system containing a nonionic surfactant and a buffer.
- concentration of the surfactant in the surfactant blank measurement system is, for example, ⁇ to ⁇ . (%.
- the surfactant blank measurement system may be an acidic system ( ⁇ 3.0 to 5 5), it is preferable to adjust to either neutral system (around ⁇ 7.0) or alkaline system ( ⁇ 8.5 to 1100) .
- the buffer used for this should be adjusted to the pH to be set.
- the neutral blank measuring system for items that are analyzed in response to an acid-reaction system containing a surfactant, such as albumin, the surfactant blank measurement system is constructed as an acid- [reaction system].
- the surfactant plank measurement system is constructed as a neutral system and to perform blank correction based on both the results of the blank measurement system and the results of the acid I / raw blank measurement system.
- items to be analyzed in an alkaline reaction system containing a surfactant are based on both the results of the neutral surfactant blank measurement system and the results of the alkaline blank measurement system. Blank correction may be performed.
- nonion-based surfactant examples include an athenole type, an athenole estenole type, an esthenole type, and a brush-containing type.
- ether-type surfactant examples include polyoxetylene olenoquinolate ether, polyoxetylene secondary alcohol ether, polyoxyethylene alkynolephenineoleatene, polyoxyethylene sterolenoateatene, Examples include polyoxyethylene lanolin derivatives, ethylene oxide derivatives of alkylphenol formalin condensates, polyoxyethylene polyoxypropylene block polymers, and polyoxyethylene polyoxypropylene alkynoleethers.
- ether ester type surfactant examples include polyoxyethylene glycerin fatty acid ester, polyoxyethylene castor oil, polyoxyethylene hard castor oil, polyoxyethylene sorbitan fatty acid ester, and polyoxyethylene sorbitol fatty acid ester.
- ester type surfactant examples include polyethylene glycol fatty acid ester, fatty acid monoglyceride, polyglycerin fatty acid ester, sonolebitan fatty acid ester, propylene glycol fatty acid ester, and sucrose fatty acid ester.
- the nitrogen-containing surfactant examples include fatty acid alkanolamides, polyoxyethylene fatty acid amides, polyoxetylene alkylamines, and alkylamine oxides.
- the sample can typically include urine or blood.
- analysis items such as albumin (Alb), total bilirubin (T-Bil), inorganic phosphorus (IP), Glucose (Glu), uric acid (UA), urea nitrogen (BUN;), aspartate aminotransferase.
- G0T alanine aminotransferase
- GPT creatine phosphoki i-ase
- CPK creatine phosphoki i-ase
- Amy amylase
- GGT Gamma daltamyl transpeptidase
- TP total protein
- TP calcium
- LDH lactate dehydrogenase
- ALP alfa phosphatase
- Mg magnesium
- FAA fructosamine
- T-Cho total cholesterol
- HDL-Cho high-density cholesterol
- '1' umbrella TG in triglycerides.
- the blank measurement performed in the neutral blank measurement system is corrected for Alb, T-Bil or IP based on the result of the blank measurement performed in the blank measurement system.
- Glu, UA, BUN, GOT, GPT, CPK, Amy, GGT or Cre are corrected based on the results of the above, and TP, Ca, LDH, Make corrections to ALP, Mg or FRA to create a surfactant blank measurement system! It is preferable to make corrections for T-Cho, TG, HDL-Cho or Alb based on the results of the blank measurement performed in advance.
- the analysis tool of the present invention may be configured as having a plurality of flow paths for moving a sample.
- An analysis reagent section or a blank measurement reagent section is provided inside each channel.
- the flow path may be configured to move the sample by capillary action, or may be configured to move the sample using the power of an electrophoresis, a micro knob, or a pump.
- the plurality of flow paths are connected to, for example, one sample liquid inlet. Of course, it is also possible to provide a plurality of sample liquid inlets so that each channel is connected to a different sample liquid inlet.
- the analysis tool of the present invention can also be configured so that a sample is directly spotted on the analysis reagent section or the blank measurement reagent section.
- the analysis reagent section and the blank reagent section are formed, for example, by holding a reagent or the like on an absorbent carrier fixed on a substrate.
- a sheet or ⁇ -starved form is used.
- Materials for forming 3 ⁇ 43 ⁇ 4 include, for example, polyethylene terephthalate, polyethylene, polypropylene, polyethylene, polyvinyl chloride, polyvinylidene chloride, Polystyrene is mentioned.
- a porous material in the form of a sheet or a film is used as the raw material for absorption “I”. Examples of the porous material include a paper-like material, a foam (foam), and a woven fabric-like material.
- Materials for forming the absorbent carrier include, for example, cotton, hemp, cellulose, nitrocellulose, cellulose acetate, rock wool, glass! ⁇ Lute, silica fiber, and carbon.
- fiber boron fiber
- polyamide aramide
- polyvinyl alcohol polyvinyl acetate
- rayon polyester
- polyolefin polyolefin.
- a rectangle, a long rectangle, a circle or an ellipse is generally used.
- FIG. 1 is an overall view showing an analysis tool according to a first embodiment of the present invention.
- FIG. 2 is a sectional view taken along the line II-II in FIG.
- FIG. 3 is a perspective view of the analysis tool shown in FIG.
- Figure 4 shows the analysis items and their abbreviations that can be analyzed with the analytical tool shown in Figure 1.
- FIG. 5 is a list showing a plurality of blank measurement systems constructed on the analysis tool shown in FIG. 1 and analysis items corresponding to each blank measurement system.
- FIG. 6 is a schematic diagram showing a state where the analysis tool shown in FIG. 1 is mounted on an analyzer.
- FIG. 7 is an overall perspective view showing the analysis tool according to the second embodiment of the present invention.
- FIG. 8 is an overall view showing an analysis tool according to a third embodiment of the present invention.
- FIG. 9 is an overall # 1 view showing an analysis tool according to a fourth embodiment of the present invention.
- the analytical tool 1 shown in FIGS. 1 to 3 is configured so that the sample can be moved by utilizing the capillary phenomenon and the sample can be analyzed by using a force optical method.
- the analysis tool 1 has a substrate 2 and a cover 3 laminated on the substrate 2.
- the substrate 2 is formed in a transparent disk shape, and has a liquid receiving portion 20 provided at a central portion, a plurality of flow paths 21 extending radially from the liquid receiving portion 20 toward the peripheral edge of the substrate 2, have.
- the liquid receiving section 20 is for holding the sample supplied to the analysis tool 1 for introduction into each flow path 21.
- the liquid receiving portion 20 is formed as a circular concave portion on the upper surface 22 of the substrate 2.
- Each flow path 21 is for moving a sample, and is formed on the upper surface 22 of the substrate 2.
- Each flow path 21 communicates with the liquid receiving section 20 and is open on the peripheral side surface of the analysis tool 1.
- Each flow path 21 has a reaction section 23, and a portion of each flow path 21 excluding the reaction section 23 has a substantially uniform rectangular cross section.
- the width and depth of the rectangular cross section of each flow path 21 are, for example, 10 to 500 ⁇ m and 5 to 500 ⁇ m, and the width / depth is 0.5 or more.
- the reaction section 23 has a larger cross-sectional area than the main cross section of the flow path 21.
- the individual reaction sections 23 are provided on the same circumference.
- Each reaction section 23 is provided with an analysis reagent section 24a to 24e or a blank reagent section 25a to 25d.
- the analysis reagents 24 a to 24 e and the blank measurement reagents 25 a to 25 d are used for analysis.
- the analysis reagents 24 a to 24 e are used to react with specific components in the sample to develop color. It is a solid that dissolves when the sample is supplied.
- 21 types of analysis reagent sections 24 a to 24 e having different compositions are provided so that a plurality of items can be analyzed in the analysis tool 1.
- the analysis reagent section 24a is for analyzing T-Bil or IP, and is configured to construct an acidic reaction system (for example, 3.0 to 5.5) when a sample is supplied. It has been done.
- the analysis reagent section 24b is for analyzing Glu, UA, BUN, G0T, GPT, CPK, Amy, GGT, or Cre, and when a sample is supplied, a neutral reaction system (for example, pH7 .0).
- the analysis reagent section 24c is used to analyze TP, Ca, LDH, ALP, Mg, or FRA. It is configured to construct a strong reaction system (for example, pH 8.5 to 11.0).
- the analysis reagent sections 24d and 24e are for analyzing T-Cho, HDL-Cho, TG, and Alb, and contain a surfactant.
- Analysis reagent portion 24 d is configured to construct a reaction system neutral (e.g. P H7. 0 near) when a sample is supplied, analytical reagent portion 24 5 e, the sample is supplied Sometimes it is configured to build an acidic reaction system (eg, pH 3.0-5.5).
- Each of the analysis reagent sections 24 a to 24 e is formed by, for example, applying a material liquid containing a reagent (including a surfactant) and a buffer solution to the reaction section 23 and then drying it.
- a material liquid containing a reagent including a surfactant
- Any reagent can be used as long as it can appropriately analyze the above items, and a known reagent is used.
- a non-ionic surfactant is used so that a lipid (for example, ten-fold fat in triceride) can be converted into a reaction system and dissolved.
- the buffer for example, the reagent parts for analysis 24 a and 24 e are used in the buffer for analysis, and the reagent parts for analysis 24 b and 24 d are used in the buffer for reagents such as the reagent buffer for analysis and the reagent parts for analysis.
- CHES buffer, CAPS buffer or Good buffer is used.
- the blank measurement reagent sections 25 a to 25 d are used to correct the effects of turbidity (challenge) due to the color and lipid of the sample.
- the acid a plank measurement reagent section 25a, a neutral blank measurement reagent section 25b, an alkaline plank measurement reagent section 25c, and a surfactant blank measurement reagent section 25d
- Types of blank measurement reagents 25a-25d are frosted. That is, in the analytical tool 1,
- the blank measurement reagent sections 25a to 25d are shared for multiple analysis items, and the four blank measurement reagent sections 25a to 25d correspond to 21 types of analysis items. It is configured. to prepare three types of blank measurement reagent part 25 a ⁇ 25 c having different pH, for example hemoglobin to Pirirubin and is a component that affects the color of the sample, by connexion absorbance to a value of the reaction system P H Effect of changing wavelength peaks when analyzing samples
- the amount depends on the pH.
- the reason why the surfactant blank measuring reagent section 25d is prepared is that the rns of turbidity (challenge) of the reaction system due to the lipid differs depending on the presence or absence of the surfactant for solubilizing the lipid.
- the acid / raw blank measurement reagent section 25a is for blank measurement of albumin (Alb), total bilirubin (T-Bil), and inorganic phosphorus (IP).
- Reagent part 25b for neutral blank measurement is glucose (Glu), uric acid (UA), urea nitrogen (BUN), aspartate aminotransferase (GOT), alanine aminotransferase (GPT), creatine phosphokinase (CPK ), Amylase (Amy :), gamma daltamyl transpeptidase (GGT), and creatine (Cre) for blank measurements.
- the reagent section for the measurement of the total strength of the blank is 25 c for total protein (TP), calcium (Ca), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), magnesium (Mg;), and fructosamine (FRA) ) Is for blank measurement.
- TP total protein
- Ca calcium
- LDH lactate dehydrogenase
- ALP alkaline phosphatase
- Mg magnesium
- FFA fructosamine
- the surfactant blank measurement reagent section 25d is used for blank measurement of total cholesterol (T-Cho), high-density cholesterol (HDL-Cho), and triglyceride fatty acid (TG).
- the blank measurement reagent sections 25a to 25c are formed, for example, by applying a buffer solution to the reaction section 23 and then drying it.
- the blank measurement reagent section 25 d is formed, for example, by applying a material liquid containing a buffer solution and a surfactant to the reaction section 23 and then drying it.
- the buffer may be, for example, a reagent for blank measurement 25a, a carboxylic acid-based buffer such as an apple buffer, or a reagent for blank measurement 25b, 25d.
- Acid buffer, blank measurement reagent section 25 c! /, Use a carbonate buffer, glycine buffer or good buffer.
- As the surfactant a nonionic surfactant force S is used as in the case of the analysis reagent sections 24 d and 24 e.
- the substrate 2 of the analytical tool 1 is made of, for example, acryl-based resin such as polymethyl methacrylate (PMMA), polystyrene (PS;), polycarbonate (PC), and polyethylene terephthalate (PET). It is formed by resin molding using such a transparent resin material.
- the liquid receiving section 20 and the plurality of flow paths 21 can be formed simultaneously with the resin molding by devising the shape of the mold.
- the inner surface of the liquid receiving section 20 and the plurality of flow paths 21 be subjected to a hydrophilic treatment.
- a hydrophilic treatment S ⁇ method For example, after a mixed gas containing fluorine gas and oxygen gas is brought into contact with each inner surface, water or water vapor is transferred to each inner surface. Re, preferably done by letting insects.
- the hydrophilic treatment is performed using gas water or the like. Hydrophilic treatment can be reliably performed even on an upright surface (side surface such as a channel) that is difficult to irradiate.
- the hydrophilic treatment of each inner surface is performed, for example, so that the contact angle force to pure water becomes ⁇ 80 degrees, more preferably 0-60 degrees.
- the cover 3 is formed in a transparent disk shape having a sample inlet 30.
- the sample introduction port 30 is used when introducing a test solution, and is formed as a through-hole and is formed.
- the sample introduction port 30 is formed in the center of the cover 3 so as to be located immediately above the liquid receiving section 20 of the substrate 2.
- This cover 3 can be formed by stretching or resin molding using a transparent resin material as in the case of FIG.
- the liquid receiving portion 20 can be formed by punching when the cover 3 is formed by stretching, and the cover 3 is formed by resin molding:
- the ⁇ can be formed simultaneously with the resin molding.
- the analysis tool 1 described above is used, for example, by attaching it to the analyzer 4 shown in FIG.
- the analyzer 4 includes a mounting section 40, a light source section 41, a light receiving section 42, a correction section 43, a calculation section 44, and a control section 45.
- the mounting portion 40 has a concave portion 40a for holding the analysis tool 1 and a light transmitting region 40b.
- the mounting section 40 is supported by a rotating shaft 40c, and the mounting section 40 is configured to rotate by rotating the rotating shaft 40c.
- the rotating shaft 40c is connected to a driving mechanism (not shown), and is controlled so as to rotate by an angle corresponding to the arrangement pitch of the reaction sections 23 in the analysis tool 1.
- the light transmission region 40b is provided at a position corresponding to the reaction part 23 of the analytical tool 1 when the analytical tool 1 is mounted in the recess 40a.
- the light i region 40b is formed by forming a target portion of the mounting portion 40 with a transparent material such as a transparent resin. Of course, the entire mounting portion 40 may be formed of a transparent material.
- the light source section 41 is for irradiating the reaction section 23 of the analysis tool 1 with light.
- the light source unit 41 is composed of, for example, a mercury lamp or a white LED. In order to use these light sources, the light from the light source unit 41, which is omitted in the drawing, is incident on the filter. After that, the reaction section 23 is irradiated with light. This is because the filter selects light having a wavelength in accordance with the light absorption characteristics of the analysis component in the reaction solution.
- the light source unit may be composed of a plurality of light sources having different peak wavelengths.
- the light receiving section 42 is for receiving the light that has passed through the reaction section 23, and is arranged coaxially with the light source section 41 with the light transmitting area 40b interposed therebetween.
- the amount of light received by the light receiving section 42 is used as a basis for analyzing a sample (for example, calculating a concentration or determining a correction value).
- the light receiving section 42 is constituted by, for example, a photodiode.
- the correction unit 43 irradiates the reaction unit 23 provided with the blank measurement reagent unit 25 a to 25 d force S with light from the light source unit 41 based on the light reception result of the light reception unit 42. This is for calculating the correction value.
- the calculation unit 44 receives the light from the light source unit 41 when the reaction unit 23 provided with the analysis reagent units 24 a to 24 e is irradiated with light from the light source unit 41. This is for performing a calculation for analyzing the sample based on the correction value calculated in 43.
- the control section 45 is for controlling the operation of each section 41 to 44.
- the correction unit 43, the calculation unit 44, and the control unit 45 can be configured by connecting a plurality of memories (for example, 0M or RAM) to one CPU, for example.
- the analysis tool 1 is mounted on the mounting section 40 of the analyzer 4 as shown in FIG.
- the sample is supplied to the analysis tool 1 through the sample inlet 30 to the liquid receiving section 20.
- the sample supplied to the liquid receiving section 20 advances each flow path toward the peripheral edge of the analysis tool 1 by capillary action.
- the sample is supplied to the plurality of reaction sections 23 at a time.
- each reaction section 23 the analysis reagent sections 24a to 24e or the blank measurement reagent sections 25a to 25d are dissolved by the sample.
- a liquid phase reaction system is constructed in the reaction section 23 provided with the analysis reagent sections 24a to 24e.
- the sample and the reagent react with each other.
- the reaction in the liquid phase reaction system shows a color that is correlated with the amount of the component to be detected in the sample, or a reactant corresponding to the amount of the component to be detected is formed. Generate.
- the liquid-phase reaction system constructed in the reaction section 23 exhibits a light-transmitting property (light-absorbing property) corresponding to the amount of the detected component.
- a rank measurement system is constructed. More specifically, in the reaction section 23 provided with the blank measurement reagent section 25a, for example, an acidic blank measurement system having a pH of 4.0 is constructed, and a plank measurement reagent section 25b is provided. In the reaction section 23, for example, a neutral blank measurement system having a pH of 7.0 is constructed. A measurement system is constructed, and in the reaction section 23 provided with the blank measurement reagent section 25 d, for example, ⁇ 7.0, a surfactant blank measurement system including a surfactant is constructed. Is done.
- the light source unit 41 irradiates the reaction unit 23 with light.
- the light irradiation by the light source unit 41 and the reception of the transmitted light by the light receiving unit 42 are performed for all the reaction units 23 set in each flow path 21 while rotating the mounting unit 40 by a fixed angle.
- a correction value is calculated based on the amount of light in the reaction unit 23 provided with the blank measurement reagent units 25a to 25d. More specifically, the correction unit 43 calculates a correction value for T-Bil and IP based on the amount of transmitted light from the reaction unit 23 provided with the blank measurement reagent unit 25a.
- the correction values for Glu, UA, BUN, G0T, GPT, CPK, Amy, GGT and re were calculated.
- the calculation unit 44 analyzes the sample based on the transmitted light amount in the reaction unit 23 provided with the analysis reagent units 24a to 24e and the calculation result of the correction information in the correction unit 43. For example, the calculation of the concentration of the detected component or the presence or absence of the detected component is performed. More specifically, for example, the amount of light received by the light receiving section 42 (or the absorbance obtained from the light receiving section 42) is multiplied by a correction value, and the corrected value is applied to a previously checked calibration curve.
- the density of the component is calculated, and the presence or absence of the carried component is determined by judging whether or not the value after correction is larger than a predetermined threshold value.
- a plurality of analysis items are grouped into four groups according to the type of the reaction system (in this embodiment, the presence or absence of a surfactant and a surfactant), and the analysis items constituting each group are described below.
- the blank measurement is shared. Therefore, the number of blank measurements that would normally be required in accordance with the number of measurement items is sufficient only by the number of analysis item groups. For example, in the present embodiment, while the number of analysis items is 21, the number of blank measurements is four.
- the number of required blanks, the amount of blank correction, and the amount of reagent required and the amount of sample required for the entire analysis are reduced. This reduces the cost of the analytical tool and reduces the burden on the subject when taking a test because the sample is a biochemical sample such as urine or blood. If the number of blank measurements is reduced, the blank measurement area to be set in the analysis tool 1 can be reduced as a whole, and the analysis tool 1 can be reduced in size.
- the analysis tool 1 of the present embodiment is configured to be able to analyze a sample using transmitted light
- one of the substrate 2 and the cover 3 is made opaque and specularly reflected light or scattered light is used. It may be possible to analyze the sample by using The arrangement of the plurality of flow paths 21 does not necessarily have to be radial.
- the analysis tool 5 shown in FIG. 7 has a configuration in which a plurality of analysis reagent pads 51 a to 51 e and a plurality of blank measurement pads 52 a to 52 d are arranged on a matrix 50 on a matrix.
- the analysis tool 5 may be configured so that the sample 50 is formed transparent and the sample is analyzed based on the transmitted light, or the sample 50 is formed opaque and the sample 50 is formed based on specular reflection light or scattered light. Alternatively, it may be configured to perform the analysis of the sample.
- Each of the analysis reagent pads 51a to 51e contains, for example, a reagent corresponding to an analysis item.
- the analysis reagent pad 51a relates to the analysis items (except Alb) measured in the acid reaction system.
- the analysis reagent pad 51b relates to an analysis item measured in a neutral reaction system.
- the analysis reagent pad 51c relates to an analysis item to be measured in an alkaline reaction system.
- the analysis reagent pad 51d relates to the analysis items (excluding Alb) measured in the surfactant reaction system.
- Analytical reagent pad 51 e is for Alb (see Figure 5).
- each of the blank measurement pads 52a to 52d can be used as an acid raw blank measurement pad 52a and a neutral plank measurement system that can construct an acid blank measurement system when a sample is supplied.
- Neutral blank measuring pad 52b which can be used to build a neutral blank measuring system
- acid I "raw blank measuring pad 52c which can be used to construct a neutral blank measuring system. It consists of four types of surfactant blank measurement pads 52d that can construct a measurement system (see Fig. 5).
- Analysis tool 5 is also configured so that multiple analysis items can be grouped into four groups according to the attributes and composition of the reaction system, and blank measurements can be shared for the analysis items that make up each group. ing. Therefore, in the analysis tool 5, the effects of the analysis tool 1 (see FIG. 1 and FIG. 3) described in the first embodiment of the present invention can be enjoyed.
- the analytical tool 5A (5B to 5D) shown in FIG. 8 includes a plurality of analytical reagent pads 51a (51b to 51e) and one plank measuring pad 52a ( 52b to 52d) have a fixed configuration.
- a plurality of analytical reagent pads 51a (51b-51e) corresponding to the analytical items similar to the reaction system, and these analytical items The blank measurement pads 52a (52b to 52d) corresponding to the above are grouped together.
- Each of the analysis tools 5A to 5D may be used individually, or the four analysis tools 5A to 5D shown in the figure may be used as one set.
- the analysis tools 5A to 5D are configured so that the blank measurement can be shared according to the attributes and composition of the reaction system, and therefore, the first embodiment of the present invention
- the effect of the analysis tool 1 (see FIGS. 1 to 3) described in (1) can be enjoyed.
- the analysis tool 5E shown in FIG. 9 has a configuration in which a plurality of analysis reagent pads 51a, 51d, 51e and two blank measurement pads 52a, 52d are fixed on the substrate 50. are doing.
- the analysis tool 5E has a configuration in which the analysis tools 5A and 5D shown in FIG. 8 are combined, and is composed of an acid I 1 raw system, a surfactant system, and an active surfactant system.
- the analysis items of three types of reaction systems are collected in one analysis tool 5E. Accordingly, the analysis tool 5E has a configuration in which an acid blank measurement pad 52a and a surfactant blank measurement pad 52d are provided.
- reaction system is classified by focusing on the pH (acid, neutral, or alkaline) of the reaction system, the presence or absence of the surfactant, and the conditions of the reaction.
- the method of classification is not limited to the example described above, and other classification methods may be used.
- the analysis tool 1 in the form described with reference to FIGS. 1 to 3 one or two of the analysis tools 5A to 5D and 5E shown in FIGS.
- the configuration may be such that two blank measurement systems are used in common.
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- Biomedical Technology (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/532,953 US20060046296A1 (en) | 2002-10-28 | 2003-10-24 | Method of correction at sample analysis, analyzer and analytical equipment |
AU2003275666A AU2003275666A1 (en) | 2002-10-28 | 2003-10-24 | Method of correction at sample analysis, analyzer and analytical equipment |
EP03758905A EP1557660A1 (en) | 2002-10-28 | 2003-10-24 | Method of correction at sample analysis, analyzer and analytical equipment |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002-312960 | 2002-10-28 | ||
JP2002312960A JP3927110B2 (ja) | 2002-10-28 | 2002-10-28 | 試料分析時の補正方法、および分析用具 |
Publications (1)
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WO2004038392A1 true WO2004038392A1 (ja) | 2004-05-06 |
Family
ID=32171154
Family Applications (1)
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PCT/JP2003/013669 WO2004038392A1 (ja) | 2002-10-28 | 2003-10-24 | 試料分析時の補正方法、分析装置および分析用具 |
Country Status (6)
Country | Link |
---|---|
US (1) | US20060046296A1 (ja) |
EP (1) | EP1557660A1 (ja) |
JP (1) | JP3927110B2 (ja) |
CN (1) | CN100533128C (ja) |
AU (1) | AU2003275666A1 (ja) |
WO (1) | WO2004038392A1 (ja) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8354073B2 (en) | 2004-06-04 | 2013-01-15 | Kyushu University, National University Corporation | Inspection chip equipped with a light amplifier element |
AT504919B1 (de) * | 2007-02-15 | 2008-09-15 | Nanoident Technologies Ag | Durchlichtmessvorrichtung |
WO2008136273A1 (ja) | 2007-04-27 | 2008-11-13 | Arkray, Inc. | ビリルビン測定方法及びビリルビン測定に用いる分析用具 |
US20110318728A1 (en) * | 2008-12-30 | 2011-12-29 | Huan Lac Phan | Systems, devices, methods and kits for fluid handling |
JP2011179825A (ja) * | 2010-02-26 | 2011-09-15 | Hitachi High-Technologies Corp | 自動分析装置 |
WO2012004723A1 (en) * | 2010-07-05 | 2012-01-12 | Koninklijke Philips Electronics N.V. | Examination system with sample incubation |
JP2014124824A (ja) * | 2012-12-26 | 2014-07-07 | Canon Inc | インクジェット記録方法、およびインクジェット記録装置 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0735744A (ja) * | 1993-07-16 | 1995-02-07 | Fujisawa Pharmaceut Co Ltd | 尿の分析方法 |
JPH11326320A (ja) * | 1998-05-19 | 1999-11-26 | Wako Pure Chem Ind Ltd | 判定色調表 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3526480A (en) * | 1966-12-15 | 1970-09-01 | Xerox Corp | Automated chemical analyzer |
US4485176A (en) * | 1982-06-28 | 1984-11-27 | E. I. Du Pont De Nemours & Company | Turbidimetric method for measuring protein in urine and cerebrospinal fluid |
US5508200A (en) * | 1992-10-19 | 1996-04-16 | Tiffany; Thomas | Method and apparatus for conducting multiple chemical assays |
JP2002202310A (ja) * | 2000-10-27 | 2002-07-19 | Morinaga Milk Ind Co Ltd | 物質の検出試薬及び検出方法 |
-
2002
- 2002-10-28 JP JP2002312960A patent/JP3927110B2/ja not_active Expired - Fee Related
-
2003
- 2003-10-24 US US10/532,953 patent/US20060046296A1/en not_active Abandoned
- 2003-10-24 CN CNB200380102260XA patent/CN100533128C/zh not_active Expired - Fee Related
- 2003-10-24 AU AU2003275666A patent/AU2003275666A1/en not_active Abandoned
- 2003-10-24 WO PCT/JP2003/013669 patent/WO2004038392A1/ja active Application Filing
- 2003-10-24 EP EP03758905A patent/EP1557660A1/en not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0735744A (ja) * | 1993-07-16 | 1995-02-07 | Fujisawa Pharmaceut Co Ltd | 尿の分析方法 |
JPH11326320A (ja) * | 1998-05-19 | 1999-11-26 | Wako Pure Chem Ind Ltd | 判定色調表 |
Also Published As
Publication number | Publication date |
---|---|
AU2003275666A1 (en) | 2004-05-13 |
US20060046296A1 (en) | 2006-03-02 |
JP2004150803A (ja) | 2004-05-27 |
CN1708681A (zh) | 2005-12-14 |
CN100533128C (zh) | 2009-08-26 |
JP3927110B2 (ja) | 2007-06-06 |
EP1557660A1 (en) | 2005-07-27 |
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