WO2004027080A3 - Procede d'extraction d'acides nucleiques a partir d'echantillons microbiens - Google Patents

Procede d'extraction d'acides nucleiques a partir d'echantillons microbiens Download PDF

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Publication number
WO2004027080A3
WO2004027080A3 PCT/US2003/029663 US0329663W WO2004027080A3 WO 2004027080 A3 WO2004027080 A3 WO 2004027080A3 US 0329663 W US0329663 W US 0329663W WO 2004027080 A3 WO2004027080 A3 WO 2004027080A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
extracting nucleic
sample
microbial samples
methods
Prior art date
Application number
PCT/US2003/029663
Other languages
English (en)
Other versions
WO2004027080A2 (fr
Inventor
Jingliang Ju
Original Assignee
Embiosis Pharmaceuticals
Jingliang Ju
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Embiosis Pharmaceuticals, Jingliang Ju filed Critical Embiosis Pharmaceuticals
Priority to AU2003270814A priority Critical patent/AU2003270814A1/en
Publication of WO2004027080A2 publication Critical patent/WO2004027080A2/fr
Publication of WO2004027080A3 publication Critical patent/WO2004027080A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés d'extraction d'acides nucléiques à partir d'échantillons provenant de l'environnement, tels que des échantillons de sol. Ces procédés consistent à obtenir des microbes intacts par lavage de l'échantillon de façon que l'acide humique soit éliminé de l'échantillon avant rupture des cellules et libération de leur contenu dans l'échantillon. Selon ces procédés, on obtient des rendements plus élevés et des acides nucléiques plus purs à partir d'échantillons prélevés dans l'environnement.
PCT/US2003/029663 2002-09-20 2003-09-19 Procede d'extraction d'acides nucleiques a partir d'echantillons microbiens WO2004027080A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003270814A AU2003270814A1 (en) 2002-09-20 2003-09-19 Method of extracting nucleic acids from microbial samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41244302P 2002-09-20 2002-09-20
US60/412,443 2002-09-20

Publications (2)

Publication Number Publication Date
WO2004027080A2 WO2004027080A2 (fr) 2004-04-01
WO2004027080A3 true WO2004027080A3 (fr) 2004-09-16

Family

ID=32030868

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/029663 WO2004027080A2 (fr) 2002-09-20 2003-09-19 Procede d'extraction d'acides nucleiques a partir d'echantillons microbiens

Country Status (2)

Country Link
AU (1) AU2003270814A1 (fr)
WO (1) WO2004027080A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2004048398A1 (ja) * 2002-11-28 2006-03-23 アークレイ株式会社 核酸の濃縮精製方法および装置
WO2005121336A1 (fr) * 2004-06-08 2005-12-22 Toagosei Co., Ltd. Procédé de purification de l'adn environnemental et procédé de dépistage efficace du gène codant la protéine appartenant à l'adn environnemental
CN100427598C (zh) * 2006-06-12 2008-10-22 南京大学 湖泊沉积物中微生物总dna的提取及生物量的鉴定方法
CN101220390B (zh) * 2007-12-18 2010-12-15 天津市农业科学院中心实验室 一种快速提取植物样本dna的方法
WO2015154013A1 (fr) * 2014-04-03 2015-10-08 The Board Of Regents For Oklahoma State University Système et procédé pour l'extraction d'adn d'échantillons contenant des acides humiques
CN107400675B (zh) * 2017-08-29 2021-01-26 海南省农业科学院植物保护研究所 一种抗瓜类枯萎病活性产物基因的克隆与序列分析方法
CN111333861B (zh) * 2020-03-03 2021-10-22 山东省农业科学院农业资源与环境研究所 一种风化煤中水溶性腐植酸盐的提取液及其提取方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TEBBE C.C.: "Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 59, no. 8, August 1993 (1993-08-01), pages 2657 - 2665, XP002978515 *

Also Published As

Publication number Publication date
WO2004027080A2 (fr) 2004-04-01
AU2003270814A8 (en) 2004-04-08
AU2003270814A1 (en) 2004-04-08

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