WO2004022764A3 - Utilisation de malate deshydrogenase pour la regeneration de nicotinanide adenine dinucleotide hydrogene (nadh) - Google Patents

Utilisation de malate deshydrogenase pour la regeneration de nicotinanide adenine dinucleotide hydrogene (nadh) Download PDF

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Publication number
WO2004022764A3
WO2004022764A3 PCT/EP2003/008631 EP0308631W WO2004022764A3 WO 2004022764 A3 WO2004022764 A3 WO 2004022764A3 EP 0308631 W EP0308631 W EP 0308631W WO 2004022764 A3 WO2004022764 A3 WO 2004022764A3
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WO
WIPO (PCT)
Prior art keywords
malate dehydrogenase
nad
preparation
nadh regeneration
enzyme
Prior art date
Application number
PCT/EP2003/008631
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English (en)
Other versions
WO2004022764A9 (fr
WO2004022764A2 (fr
Inventor
Shukrallah Naamnieh
Werner Hummel
Harald Groeger
Original Assignee
Degussa
Shukrallah Naamnieh
Werner Hummel
Harald Groeger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa, Shukrallah Naamnieh, Werner Hummel, Harald Groeger filed Critical Degussa
Priority to EP03793686A priority Critical patent/EP1534849A2/fr
Priority to CA002497499A priority patent/CA2497499A1/fr
Priority to JP2004533320A priority patent/JP2005537017A/ja
Priority to AU2003255368A priority patent/AU2003255368A1/en
Publication of WO2004022764A2 publication Critical patent/WO2004022764A2/fr
Publication of WO2004022764A3 publication Critical patent/WO2004022764A3/fr
Publication of WO2004022764A9 publication Critical patent/WO2004022764A9/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C12N9/0018—Phenylalanine dehydrogenase (1.4.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/36—Dinucleotides, e.g. nicotineamide-adenine dinucleotide phosphate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/002—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by oxidation/reduction reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention, qui a trait à un procédé permettant de préparer des composés organiques énantiomériquement enrichis, concerne notamment un mode opératoire par voie enzymatique dans le cadre duquel, dans un système couplé de réaction enzymatique, NAD (P) H est digéré par une enzyme aux fins de la préparation dudit composé organique et simultanément régénéré par un second système de réaction. L'invention porte également sur un système de réaction agissant comme décrit et sur un avantageux catalyseur de cellule totale.
PCT/EP2003/008631 2002-09-03 2003-08-05 Utilisation de malate deshydrogenase pour la regeneration de nicotinanide adenine dinucleotide hydrogene (nadh) WO2004022764A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP03793686A EP1534849A2 (fr) 2002-09-03 2003-08-05 Utilisation de malate deshydrogenase pour la regeneration de nicotinanide adenine dinucleotide hydrogene (nadh)
CA002497499A CA2497499A1 (fr) 2002-09-03 2003-08-05 Utilisation de malate deshydrogenase pour la regeneration de nicotinanide adenine dinucleotide hydrogene (nadh)
JP2004533320A JP2005537017A (ja) 2002-09-03 2003-08-05 Ｎａｄｈ再生のためのリンゴ酸デヒドロゲナーゼの使用
AU2003255368A AU2003255368A1 (en) 2002-09-03 2003-08-05 Use of malate dehydrogenase for nadh regeneration

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10240603A DE10240603A1 (de) 2002-09-03 2002-09-03 Verwendung von Malat-Dehydrogenase zur NADH-Regenerierung
DE10240603.0 2002-09-03

Publications (3)

Publication Number Publication Date
WO2004022764A2 WO2004022764A2 (fr) 2004-03-18
WO2004022764A3 true WO2004022764A3 (fr) 2004-10-21
WO2004022764A9 WO2004022764A9 (fr) 2005-04-14

Family

ID=31502323

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/008631 WO2004022764A2 (fr) 2002-09-03 2003-08-05 Utilisation de malate deshydrogenase pour la regeneration de nicotinanide adenine dinucleotide hydrogene (nadh)

Country Status (6)

Country Link
EP (1) EP1534849A2 (fr)
JP (1) JP2005537017A (fr)
AU (1) AU2003255368A1 (fr)
CA (1) CA2497499A1 (fr)
DE (1) DE10240603A1 (fr)
WO (1) WO2004022764A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10313972A1 (de) * 2003-03-27 2004-10-21 Degussa Ag Gekoppeltes cofaktorabhängiges enzymatisches Reaktionssystem
DE102004008445A1 (de) 2004-02-19 2005-09-08 Degussa Ag Verfahren zur Herstellung von L-Aminosäuren aus D-Aminosäuren
DE102004014280A1 (de) * 2004-03-22 2005-10-27 Degussa Ag Verfahren zur Herstellung von optisch aktiven Aminosäuren mittels eines Ganzzellkatalysators
DE102004028407A1 (de) * 2004-06-14 2005-12-29 Degussa Ag Herstellung optisch aktiver Alkohole mit Hilfe von Ganzzellkatalysatoren
DE102004038054A1 (de) * 2004-08-05 2006-03-16 Maxens Gmbh Verfahren zur Herstellung primärer Alkohole
TW201343623A (zh) 2012-02-07 2013-11-01 Annikki Gmbh 使氧化還原輔因子經酶催化再生之方法
WO2013117251A1 (fr) 2012-02-07 2013-08-15 Annikki Gmbh Procédé de régénération enzymatique de cofacteurs redox

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61128895A (ja) * 1984-11-26 1986-06-16 Takara Shuzo Co Ltd 補酵素再生反応を利用した有用物質の製造方法
JPH01285193A (ja) * 1988-05-12 1989-11-16 Daicel Chem Ind Ltd Ｄ−アスパラギン酸の製造方法
EP0385415A1 (fr) * 1989-02-28 1990-09-05 Mitsubishi Petrochemical Co., Ltd. Procédé de préparation de NADH oxydase
WO1999046363A1 (fr) * 1998-03-11 1999-09-16 Valtion Teknillinen Tutkimuskeskus Micro-organismes transformes a proprietes ameliorees
US20020042110A1 (en) * 1998-12-21 2002-04-11 Daicel Chemical Industries, Ltd., A Japanese Corporation Novel carbonyl reductase, method for producing said enzyme, DNA encoding said enzyme, and method for producing alcohol using said enzyme

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61128895A (ja) * 1984-11-26 1986-06-16 Takara Shuzo Co Ltd 補酵素再生反応を利用した有用物質の製造方法
JPH01285193A (ja) * 1988-05-12 1989-11-16 Daicel Chem Ind Ltd Ｄ−アスパラギン酸の製造方法
EP0385415A1 (fr) * 1989-02-28 1990-09-05 Mitsubishi Petrochemical Co., Ltd. Procédé de préparation de NADH oxydase
WO1999046363A1 (fr) * 1998-03-11 1999-09-16 Valtion Teknillinen Tutkimuskeskus Micro-organismes transformes a proprietes ameliorees
US20020042110A1 (en) * 1998-12-21 2002-04-11 Daicel Chemical Industries, Ltd., A Japanese Corporation Novel carbonyl reductase, method for producing said enzyme, DNA encoding said enzyme, and method for producing alcohol using said enzyme

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BOMMARIUS A S ET AL: "BIOCATALYSIS TO AMINO ACID-BASED CHIRAL PHARMACEUTICAL-EXAMPLES AND PERSPECTIVES", JOURNAL OF MOLECULAR CATALYSIS. B, ENZYMATIC, ELSEVIER, AMSTERDAM,, NL, vol. 5, 1998, pages 1 - 11, XP002929513, ISSN: 1381-1177 *
DATABASE WPI Section Ch Week 198630, Derwent World Patents Index; Class B05, AN 1986-194622, XP002264718 *
DATABASE WPI Section Ch Week 199001, Derwent World Patents Index; Class B05, AN 1990-003179, XP002264717 *
GU K F ET AL: "PRODUCTION OF ESSENTIAL L BRANCHED-CHAIN AMINO ACIDS IN BIOREACTORS CONTAINING ARTIFICIAL CELLS IMMOBILIZED MULTIENZYME SYSTEMS AND DEXTRAN-NAD", BIOTECHNOLOGY AND BIOENGINEERING, vol. 36, no. 3, 1990, pages 263 - 269, XP001156578, ISSN: 0006-3592 *
SUYE S: "COENZYME REGENERATION WITH MALIC ENZYME REACTION SYSTEM", RECENT RESEARCH DEVELOPMENTS IN FERMENTATION AND BIOENGINEERING, XX, XX, vol. 1, no. 1, 1998, pages 55 - 64, XP000982890 *

Also Published As

Publication number Publication date
EP1534849A2 (fr) 2005-06-01
AU2003255368A1 (en) 2004-03-29
JP2005537017A (ja) 2005-12-08
CA2497499A1 (fr) 2004-03-18
WO2004022764A9 (fr) 2005-04-14
WO2004022764A2 (fr) 2004-03-18
DE10240603A1 (de) 2004-03-11
AU2003255368A8 (en) 2004-03-29

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