WO2004003216A3 - Source n hydrolysee - Google Patents

Source n hydrolysee Download PDF

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Publication number
WO2004003216A3
WO2004003216A3 PCT/DK2003/000441 DK0300441W WO2004003216A3 WO 2004003216 A3 WO2004003216 A3 WO 2004003216A3 DK 0300441 W DK0300441 W DK 0300441W WO 2004003216 A3 WO2004003216 A3 WO 2004003216A3
Authority
WO
WIPO (PCT)
Prior art keywords
source
enzyme
fermentation medium
hydrolysed
sterilization
Prior art date
Application number
PCT/DK2003/000441
Other languages
English (en)
Other versions
WO2004003216A2 (fr
Inventor
Mogens Wuempelmann
Niels Banke
Soeren Michaelsen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Priority to AU2003236810A priority Critical patent/AU2003236810A1/en
Priority to EP03735323A priority patent/EP1520011A2/fr
Publication of WO2004003216A2 publication Critical patent/WO2004003216A2/fr
Publication of WO2004003216A3 publication Critical patent/WO2004003216A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé de production d'une enzyme d'intérêt, à l'échelle industrielle, qui consiste: a) à faire fermenter une souche microbienne produisant une enzyme d'intérêt dans un milieu de fermentation comprenant au moins une source N complexe préhydrolysée, lesdites sources N étant stérilisées séparément de toute autre source contenant des glucides et la préhydrolyse étant réalisée par addition d'un acide et/ou d'une enzyme hydrolytique; et b) à récupérer l'enzyme d'intérêt du bouillon de fermentation.
PCT/DK2003/000441 2002-07-01 2003-06-26 Source n hydrolysee WO2004003216A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003236810A AU2003236810A1 (en) 2002-07-01 2003-06-26 Sterilization of a fermentation medium comprising hydrolysed n-source
EP03735323A EP1520011A2 (fr) 2002-07-01 2003-06-26 Sterilisation d'une culture de fermentation contenant source n hydrolysee

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200201021 2002-07-01
DKPA200201021 2002-07-01
DKPA200201838 2002-11-28
DKPA200201838 2002-11-28

Publications (2)

Publication Number Publication Date
WO2004003216A2 WO2004003216A2 (fr) 2004-01-08
WO2004003216A3 true WO2004003216A3 (fr) 2004-03-18

Family

ID=30001780

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000441 WO2004003216A2 (fr) 2002-07-01 2003-06-26 Source n hydrolysee

Country Status (4)

Country Link
EP (1) EP1520011A2 (fr)
CN (1) CN1665924A (fr)
AU (1) AU2003236810A1 (fr)
WO (1) WO2004003216A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100766474B1 (ko) * 2000-12-20 2007-10-15 교와 핫꼬 케미칼 가부시키가이샤 금속착체형 스쿠아리리움 화합물 및 이를 이용한 광기록매체
CN102660484B (zh) * 2012-05-18 2014-06-04 山东大学 一株固氮红细菌及其培养方法与应用
US20150291656A1 (en) 2012-11-01 2015-10-15 Novozymes A/S Method for removal of dna
CN105018550A (zh) * 2015-07-16 2015-11-04 合肥平光制药有限公司 一种高nad积累量发酵液的生产方法
BR112018011567A2 (pt) 2015-12-09 2018-11-27 Basf Se método de purificação de uma proteína de interesse, e, uso de um método.
US20190292514A1 (en) 2016-07-14 2019-09-26 Basf Se Fermentation medium comprising chelating agent
EP3983425A1 (fr) 2019-06-13 2022-04-20 Basf Se Procédé de récupération d'une protéine à partir d'un bouillon de fermentation à l'aide d'un cation divalent
WO2021001297A1 (fr) 2019-07-02 2021-01-07 Basf Se Procédé de préparation d'un milieu de fermentation
WO2021004830A1 (fr) 2019-07-05 2021-01-14 Basf Se Procédé de fermentation industrielle pour cellules microbiennes à l'aide d'une pré-culture par lots
WO2021122687A1 (fr) 2019-12-19 2021-06-24 Basf Se Augmentation du rendement spatio-temporel, de l'efficacité de conversion du carbone et de la flexibilité des substrat carbonés dans la production de produits chimiques fins
CN115135762A (zh) 2019-12-20 2022-09-30 巴斯夫欧洲公司 降低萜烯的毒性和增加微生物的生产潜力
BR112023001386A2 (pt) 2020-07-28 2023-02-14 Basf Se Método para cultivar uma célula hospedeira de bacillus, e, cultura de células hospedeiras de bacillus
MX2023001272A (es) 2020-07-28 2023-03-03 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de cambio de temperatura.
WO2022023371A1 (fr) 2020-07-28 2022-02-03 Basf Se Procédé de fermentation industrielle pour bacillus utilisant une récolte partielle
BR112023003778A2 (pt) 2020-09-04 2023-03-28 Basf Se Método para separar uma molécula de interesse de um agente antiespumante em um caldo de fermentação, e, processo para purificar uma molécula de interesse de um caldo de fermentação
MX2023003276A (es) 2020-09-22 2023-05-03 Basf Se Método para recuperar una proteína de un caldo de fermentación que comprende un alto grado de células lisadas.
WO2023118565A1 (fr) 2021-12-23 2023-06-29 Novozymes A/S Réduction d'adn résiduel dans des produits de fermentation microbienne
WO2024028338A1 (fr) 2022-08-02 2024-02-08 Basf Se Procédé de production de protéine stabilisée utilisant des cellules hôtes de bacillus

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4250263A (en) * 1979-11-13 1981-02-10 Uop Inc. Method of purification of thermally stable enzymes
FR2496689A1 (fr) * 1980-12-19 1982-06-25 Roquette Freres Procede et substrat de fermentation a base de proteines de pomme de terre
US4430322A (en) * 1980-11-07 1984-02-07 Merck & Co., Inc. Modified glucans as anti-caries agent and method of use
WO1992019716A1 (fr) * 1991-05-07 1992-11-12 Agro-Ferm A/S Procede d'obtention de milieux de culture a partir de seves vegetales
JPH0994093A (ja) * 1995-09-29 1997-04-08 Snow Brand Milk Prod Co Ltd Ｌ−乳酸の製造法
WO1998051163A2 (fr) * 1997-05-16 1998-11-19 Novo Nordisk Biotech, Inc. Procedes de production d'hydrolysats proteiques
WO2001005997A2 (fr) * 1999-07-16 2001-01-25 Massachusetts Institute Of Technology Methode de production de toxine tetanique par utilisation de milieux sensiblement exempts de produits d'origine animale

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4250263A (en) * 1979-11-13 1981-02-10 Uop Inc. Method of purification of thermally stable enzymes
US4430322A (en) * 1980-11-07 1984-02-07 Merck & Co., Inc. Modified glucans as anti-caries agent and method of use
FR2496689A1 (fr) * 1980-12-19 1982-06-25 Roquette Freres Procede et substrat de fermentation a base de proteines de pomme de terre
WO1992019716A1 (fr) * 1991-05-07 1992-11-12 Agro-Ferm A/S Procede d'obtention de milieux de culture a partir de seves vegetales
JPH0994093A (ja) * 1995-09-29 1997-04-08 Snow Brand Milk Prod Co Ltd Ｌ−乳酸の製造法
WO1998051163A2 (fr) * 1997-05-16 1998-11-19 Novo Nordisk Biotech, Inc. Procedes de production d'hydrolysats proteiques
WO2001005997A2 (fr) * 1999-07-16 2001-01-25 Massachusetts Institute Of Technology Methode de production de toxine tetanique par utilisation de milieux sensiblement exempts de produits d'origine animale

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 199724, Derwent World Patents Index; Class A41, AN 1997-266483, XP002231896 *
MARSHALL CAROLYNNE T ET AL: "Use of comparative reasoning tools for evaluation of the effect of sterilization conditions on fermentations to produce acidic fibroblast growth factor", BIOTECHNOLOGY AND BIOENGINEERING, vol. 47, no. 6, 1995, pages 688 - 695, XP002265089, ISSN: 0006-3592 *

Also Published As

Publication number Publication date
AU2003236810A1 (en) 2004-01-19
WO2004003216A2 (fr) 2004-01-08
CN1665924A (zh) 2005-09-07
AU2003236810A8 (en) 2004-01-19
EP1520011A2 (fr) 2005-04-06

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