WO2003072728A2 - Trans-differenciation cellulaire - Google Patents

Trans-differenciation cellulaire Download PDF

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Publication number
WO2003072728A2
WO2003072728A2 PCT/US2003/005434 US0305434W WO03072728A2 WO 2003072728 A2 WO2003072728 A2 WO 2003072728A2 US 0305434 W US0305434 W US 0305434W WO 03072728 A2 WO03072728 A2 WO 03072728A2
Authority
WO
WIPO (PCT)
Prior art keywords
cells
insulin
pancreatic endocrine
hepatic stem
pancreatic
Prior art date
Application number
PCT/US2003/005434
Other languages
English (en)
Other versions
WO2003072728A3 (fr
Inventor
Lijun Yang
Original Assignee
University Of Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Florida filed Critical University Of Florida
Priority to AU2003217658A priority Critical patent/AU2003217658A1/en
Publication of WO2003072728A2 publication Critical patent/WO2003072728A2/fr
Publication of WO2003072728A3 publication Critical patent/WO2003072728A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars

Abstract

Des cellules embryonnaires hépatiques hautement purifiées sont soumises à une trans-différenciation en cellules produisant des hormones endocrines pancréatiques par leur culture in vitro dans un milieu contenant des niveaux élevés de glucose. Ces cellules ayant subi une trans-différenciation expriment de l'insuline, le glucagon, et le polypeptide pancréatique, mais pas la protéine hépatocytaire Hep-par. Lors de leur stimulation avec du glucose, ces cellules effectuent la synthèse et la sécrétion de l'insuline, une réaction accentuée par le nicotinamide. La transplantation de ces cellules ayant subi une trans-différenciation dans un animal hyperglycémique entraîne une normalisation de la glycémie chez l'animal.
PCT/US2003/005434 2002-02-22 2003-02-24 Trans-differenciation cellulaire WO2003072728A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003217658A AU2003217658A1 (en) 2002-02-22 2003-02-24 Cellular trans-differentiation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35886402P 2002-02-22 2002-02-22
US60/358,864 2002-02-22

Publications (2)

Publication Number Publication Date
WO2003072728A2 true WO2003072728A2 (fr) 2003-09-04
WO2003072728A3 WO2003072728A3 (fr) 2004-01-15

Family

ID=27766006

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/005434 WO2003072728A2 (fr) 2002-02-22 2003-02-24 Trans-differenciation cellulaire

Country Status (3)

Country Link
US (1) US7029915B2 (fr)
AU (1) AU2003217658A1 (fr)
WO (1) WO2003072728A2 (fr)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005083059A1 (fr) * 2004-02-23 2005-09-09 University Of Florida Compositions et methodes pour fabriquer des cellules de production d'insuline
US7875273B2 (en) 2004-12-23 2011-01-25 Ethicon, Incorporated Treatment of Parkinson's disease and related disorders using postpartum derived cells
US7875272B2 (en) 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
US8318483B2 (en) 2003-06-27 2012-11-27 Advanced Technologies And Regenerative Medicine, Llc Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
US9175261B2 (en) 2005-12-16 2015-11-03 DePuy Synthes Products, Inc. Human umbilical cord tissue cells for inhibiting adverse immune response in histocompatibility-mismatched transplantation
US9611513B2 (en) 2011-12-23 2017-04-04 DePuy Synthes Products, Inc. Detection of human umbilical cord tissue derived cells
US9943552B2 (en) 2009-03-26 2018-04-17 DePuy Synthes Products, Inc. hUTC as therapy for Alzheimer's disease
US10179900B2 (en) 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
US10557116B2 (en) 2008-12-19 2020-02-11 DePuy Synthes Products, Inc. Treatment of lung and pulmonary diseases and disorders
US10744164B2 (en) 2003-06-27 2020-08-18 DePuy Synthes Products, Inc. Repair and regeneration of ocular tissue using postpartum-derived cells

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0300208D0 (en) * 2003-01-06 2003-02-05 Oxford Biomedica Ltd Insulin producing cells
US9572840B2 (en) 2003-06-27 2017-02-21 DePuy Synthes Products, Inc. Regeneration and repair of neural tissue using postpartum-derived cells
US9592258B2 (en) 2003-06-27 2017-03-14 DePuy Synthes Products, Inc. Treatment of neurological injury by administration of human umbilical cord tissue-derived cells
US9125906B2 (en) 2005-12-28 2015-09-08 DePuy Synthes Products, Inc. Treatment of peripheral vascular disease using umbilical cord tissue-derived cells
EP2155860B1 (fr) 2007-05-03 2014-08-27 The Brigham and Women's Hospital, Inc. Cellules souches multipotentes et leurs utilisations
US8574567B2 (en) * 2007-05-03 2013-11-05 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
US20100143313A1 (en) * 2008-12-10 2010-06-10 The General Hospital Corporation Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells
KR102181336B1 (ko) 2013-06-13 2020-11-24 오르제네시스 엘티디. 세포 집단, 이의 전환분화 방법 및 이용 방법
US20170248581A1 (en) * 2014-10-07 2017-08-31 NuTech Medical, Inc. Mesenchymal Stem Cell Diagnostic Testing
MA41296A (fr) 2014-12-30 2017-11-07 Orgenesis Ltd Procédés de transdifférenciation et procédés d'utilisation de ceux-ci
SG11201911256UA (en) 2017-05-08 2020-01-30 Orgenesis Ltd Transdifferentiated cell populations and methods of use thereof
US11788062B2 (en) 2017-07-27 2023-10-17 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Functional feline pancreatic cells from adipose tissue

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020182728A1 (en) * 2001-03-29 2002-12-05 Vijayakumar Ramiya Method for transdifferentiation of non pancreatic stem cells to the pancreatic differentiation pathway
US20030031657A1 (en) * 1999-12-06 2003-02-13 Massachusetts General Hospital Stem cells and their use in transplantation
US20030138951A1 (en) * 2001-10-18 2003-07-24 Li Yin Conversion of liver stem and progenitor cells to pancreatic functional cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001647A (en) * 1994-04-28 1999-12-14 Ixion Biotechnology, Inc. In vitro growth of functional islets of Langerhans and in vivo uses thereof
US5834308A (en) * 1994-04-28 1998-11-10 University Of Florida Research Foundation, Inc. In vitro growth of functional islets of Langerhans

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030031657A1 (en) * 1999-12-06 2003-02-13 Massachusetts General Hospital Stem cells and their use in transplantation
US20020182728A1 (en) * 2001-03-29 2002-12-05 Vijayakumar Ramiya Method for transdifferentiation of non pancreatic stem cells to the pancreatic differentiation pathway
US20030138951A1 (en) * 2001-10-18 2003-07-24 Li Yin Conversion of liver stem and progenitor cells to pancreatic functional cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YANG ET AL.: 'In vitro trans-differentiation of adult hepatic stem cells into pancreatic endocrine hormone-producing cells' PROC. NATL. ACAD. SCI. USA vol. 99, no. 12, 11 June 2002, pages 8078 - 8083, XP002961837 *

Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11191789B2 (en) 2003-06-27 2021-12-07 DePuy Synthes Products, Inc. Cartilage and bone repair and regeneration using postpartum-derived cells
US10195233B2 (en) 2003-06-27 2019-02-05 DePuy Synthes Products, Inc. Postpartum cells derived from placental tissue, and methods of making and using the same
US10744164B2 (en) 2003-06-27 2020-08-18 DePuy Synthes Products, Inc. Repair and regeneration of ocular tissue using postpartum-derived cells
US8318483B2 (en) 2003-06-27 2012-11-27 Advanced Technologies And Regenerative Medicine, Llc Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
US8703121B2 (en) 2003-06-27 2014-04-22 DePuy Synthes Products, LLC Postpartum-derived cells for use in treatment of disease of the heart and circulatory system
US9717763B2 (en) 2003-06-27 2017-08-01 DePuy Synthes Products, Inc. Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
US9504719B2 (en) 2003-06-27 2016-11-29 DePuy Synthes Products, Inc. Soft tissue repair and regeneration using postpartum-derived cells and cell products
US9579351B2 (en) 2003-06-27 2017-02-28 DePuy Synthes Products, Inc. Postpartum cells derived from placental tissue, and methods of making and using the same
US7875272B2 (en) 2003-06-27 2011-01-25 Ethicon, Incorporated Treatment of stroke and other acute neuraldegenerative disorders using postpartum derived cells
US10758576B2 (en) 2003-06-27 2020-09-01 DePuy Synthes Products, Inc. Soft tissue repair and regeneration using postpartum-derived cells and cell products
US10039793B2 (en) 2003-06-27 2018-08-07 DePuy Synthes Products, Inc. Soft tissue repair and regeneration using postpartum-derived cells and cell products
US11179422B2 (en) 2003-06-27 2021-11-23 DePuy Synthes Products, Inc. Method of differentiating umbilical cord tissue into a chondrogenic phenotype
US11000554B2 (en) 2003-06-27 2021-05-11 DePuy Synthes Products, Inc. Postpartum cells derived from placental tissue, and methods of making and using the same
US10500234B2 (en) 2003-06-27 2019-12-10 DePuy Synthes Products, Inc. Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
US10220059B2 (en) 2003-06-27 2019-03-05 DePuy Synthes Products, Inc. Postpartum cells derived from placental tissue, and methods of making and using the same
US10383898B2 (en) 2003-06-27 2019-08-20 DePuy Synthes Products, Inc. Postpartum cells derived from placental tissue, and methods of making and using the same
WO2005083059A1 (fr) * 2004-02-23 2005-09-09 University Of Florida Compositions et methodes pour fabriquer des cellules de production d'insuline
US7875273B2 (en) 2004-12-23 2011-01-25 Ethicon, Incorporated Treatment of Parkinson's disease and related disorders using postpartum derived cells
US9175261B2 (en) 2005-12-16 2015-11-03 DePuy Synthes Products, Inc. Human umbilical cord tissue cells for inhibiting adverse immune response in histocompatibility-mismatched transplantation
US10179900B2 (en) 2008-12-19 2019-01-15 DePuy Synthes Products, Inc. Conditioned media and methods of making a conditioned media
US10557116B2 (en) 2008-12-19 2020-02-11 DePuy Synthes Products, Inc. Treatment of lung and pulmonary diseases and disorders
US9943552B2 (en) 2009-03-26 2018-04-17 DePuy Synthes Products, Inc. hUTC as therapy for Alzheimer's disease
US10724105B2 (en) 2011-12-23 2020-07-28 DePuy Synthes Products, Inc. Detection of human umbilical cord tissue-derived cells
US9611513B2 (en) 2011-12-23 2017-04-04 DePuy Synthes Products, Inc. Detection of human umbilical cord tissue derived cells

Also Published As

Publication number Publication date
AU2003217658A1 (en) 2003-09-09
WO2003072728A3 (fr) 2004-01-15
US7029915B2 (en) 2006-04-18
AU2003217658A8 (en) 2003-09-09
US20030223974A1 (en) 2003-12-04

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