WO2003066900A3 - Mutations du gene eif4e et resistance aux potyvirus - Google Patents

Mutations du gene eif4e et resistance aux potyvirus Download PDF

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Publication number
WO2003066900A3
WO2003066900A3 PCT/FR2003/000397 FR0300397W WO03066900A3 WO 2003066900 A3 WO2003066900 A3 WO 2003066900A3 FR 0300397 W FR0300397 W FR 0300397W WO 03066900 A3 WO03066900 A3 WO 03066900A3
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WO
WIPO (PCT)
Prior art keywords
amino acid
gene mutations
eif4e gene
potyvirus resistance
represent
Prior art date
Application number
PCT/FR2003/000397
Other languages
English (en)
Other versions
WO2003066900A2 (fr
Inventor
Carole Caranta
Sandrine Ruffel
Adbelhafid Bendahmane
Alain Palloix
Christophe Robaglia
Original Assignee
Genoplante Valor
Carole Caranta
Sandrine Ruffel
Adbelhafid Bendahmane
Alain Palloix
Christophe Robaglia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FR0201583A external-priority patent/FR2835849B1/fr
Application filed by Genoplante Valor, Carole Caranta, Sandrine Ruffel, Adbelhafid Bendahmane, Alain Palloix, Christophe Robaglia filed Critical Genoplante Valor
Priority to MXPA04007718A priority Critical patent/MXPA04007718A/es
Priority to JP2003566248A priority patent/JP2006503544A/ja
Priority to NZ534545A priority patent/NZ534545A/en
Priority to EP03718850A priority patent/EP1472374A2/fr
Priority to US10/502,795 priority patent/US7919677B2/en
Priority to AU2003222893A priority patent/AU2003222893B2/en
Publication of WO2003066900A2 publication Critical patent/WO2003066900A2/fr
Publication of WO2003066900A3 publication Critical patent/WO2003066900A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne l'obtention de plantes résistantes aux potyvirus présentant une ou plusieurs mutations dans une région conservée du facteur de traduction eIF4E, définie par la séquence générale (I)suivante :DX1X2X3X4KSX5QX6AWGSSX7RX8X9YTFSX10VEX11FWX12X13YNNIHX14PSKLX15X16GAD dans laquelle :- X1, X2, X3, X4, X6, X7, X8, X9, X10, X12, X13, X15, et X16 représentent chacun un acide aminé neutre ; - X5 et X14 représentent un acide aminé basique ;- X11 représente un acide aminé acide ;- D, K, S, Q, A, W, G, R, Y, T, F, V, E, N, I, H, P, et L ont leur signification usuelle en code 1-lettre.
PCT/FR2003/000397 2002-02-08 2003-02-07 Mutations du gene eif4e et resistance aux potyvirus WO2003066900A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
MXPA04007718A MXPA04007718A (es) 2002-02-08 2003-02-07 Mutaciones del gen eif4e y resisntencia a los potyvirus.
JP2003566248A JP2006503544A (ja) 2002-02-08 2003-02-07 ｅＩＦ４Ｅ遺伝子の突然変異とポティウイルス（ｐｏｔｙｖｉｒｕｓ）抵抗性
NZ534545A NZ534545A (en) 2002-02-08 2003-02-07 EIF4E gene mutations and potyvirus resistance
EP03718850A EP1472374A2 (fr) 2002-02-08 2003-02-07 Mutations du gene eif4e et resistance aux potyvirus
US10/502,795 US7919677B2 (en) 2002-02-08 2003-02-07 eIF4E gene mutations and potyvirus resistance
AU2003222893A AU2003222893B2 (en) 2002-02-08 2003-02-07 eIF4E gene mutations and potyvirus resistance

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FR0201583A FR2835849B1 (fr) 2002-02-08 2002-02-08 Procede de selection ou d'obtention de plantes resistantes aux potyvirus et sequences "marquant" ou "conferant" cette resistance
FR02/01583 2002-02-08
FR02/13678 2002-10-31
FR0213678A FR2835698B1 (fr) 2002-02-08 2002-10-31 Procede de selection ou d'obtention de plantes resistantes aux potyvirus et sequences marquant ou codant cette resistance

Publications (2)

Publication Number Publication Date
WO2003066900A2 WO2003066900A2 (fr) 2003-08-14
WO2003066900A3 true WO2003066900A3 (fr) 2004-04-08

Family

ID=27624217

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2003/000397 WO2003066900A2 (fr) 2002-02-08 2003-02-07 Mutations du gene eif4e et resistance aux potyvirus

Country Status (8)

Country Link
US (1) US7919677B2 (fr)
EP (1) EP1472374A2 (fr)
JP (1) JP2006503544A (fr)
AU (1) AU2003222893B2 (fr)
FR (1) FR2835698B1 (fr)
MX (1) MXPA04007718A (fr)
NZ (1) NZ534545A (fr)
WO (1) WO2003066900A2 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003299666A1 (en) * 2002-12-17 2004-07-22 Cornell Research Foundation, Inc. RECESSIVE PLANT VIRAL RESISTANCE RESULTS FROM MUTATIONS IN TRANSLATION INITIATION FACTOR eIF4E
FR2870856B1 (fr) * 2004-05-25 2006-09-15 Genoplante Valor Soc Par Actio Procede de selection ou d'obtention de plantes resistantes au pvmv
FR2878532B1 (fr) * 2004-11-26 2007-03-02 Genoplante Valor Soc Par Actio Methode d'adressage d'acides nucleiques vers des plastes
ES2316298B1 (es) * 2007-09-21 2010-02-16 Consejo Superior De Investigaciones Cientificas Manipulacion en la funcion de atdbp1 para generar resistencia frente a potyvirus.
WO2010048398A2 (fr) 2008-10-22 2010-04-29 Cornell University Séquences d’eif4e mutées issues de la pomme de terre et permettant de conférer une résistance aux virus
GB201010740D0 (en) * 2010-06-25 2010-08-11 Univ Warwick A plant
EP3447065A1 (fr) * 2010-09-03 2019-02-27 Philip Morris Products S.A. Réduction des métaux lourds dans les plantes
WO2012054468A2 (fr) * 2010-10-18 2012-04-26 J.R. Simplot Company Résistance au potyvirus dans la pomme de terre
CA2881639C (fr) 2012-08-23 2024-03-19 Seminis Vegetable Seeds, Inc. Melon resistant a de multiples virus
WO2016190452A1 (fr) * 2015-05-22 2016-12-01 서울대학교산학협력단 Gène pvr4 présentant une résistance aux potyvirus et son utilisation
US10729082B2 (en) * 2017-10-16 2020-08-04 Benchbio Pvt. Ltd. Papaya with novel traits and methods for producing such papaya plants
JP7264832B2 (ja) * 2018-02-02 2023-04-25 キッコーマン株式会社 ウイルス抵抗性植物及びその作出方法
CN109371056B (zh) * 2018-09-16 2022-04-08 云南省烟草农业科学研究院 一种选育对辣椒脉斑驳病毒抗性的烟草植物的方法
CN112094942B (zh) * 2020-10-23 2023-09-12 贵州省烟草科学研究院 与烟草pvy抗性紧密连锁的分子标记、引物及应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043820A1 (fr) * 1998-02-25 1999-09-02 E.I. Du Pont De Nemours And Company FACTEUR DE TRANSLATION eIF-4E DE VEGETAUX
WO2001040490A2 (fr) * 1999-12-02 2001-06-07 Institut National De La Recherche Scientifique Inhibiteurs du facteur d'initiation 4e eucaryotique et utilisation en tant qu'herbicides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043820A1 (fr) * 1998-02-25 1999-09-02 E.I. Du Pont De Nemours And Company FACTEUR DE TRANSLATION eIF-4E DE VEGETAUX
WO2001040490A2 (fr) * 1999-12-02 2001-06-07 Institut National De La Recherche Scientifique Inhibiteurs du facteur d'initiation 4e eucaryotique et utilisation en tant qu'herbicides

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LELLIS A ET AL: "Loss-of-susceptibility mutants of Arabidopsis thaliana reveal an essential role for eIF(iso)4E during Potyvirus infection", CURRENT BIOLOGY, vol. 12, no. 12, 25 June 2002 (2002-06-25), pages 1046 - 51, XP002217535 *
LEONARD SIMON ET AL: "Complex formation between potyvirus VPg and translation eukaryotic initiation factor 4E correlates with virus infectivity", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 74, no. 17, September 2000 (2000-09-01), pages 7730 - 7737, XP002176524, ISSN: 0022-538X *
REVERS F ET AL: "NEW ADVANCES IN UNDERSTANDING THE MOLECULAR BIOLOGY OF PLANT/POTYVIRUS INTERACTIONS", MOLECULAR PLANT-MICROBE INTERACTIONS, APS PRESS, ST. PAUL, MN, US, vol. 12, no. 5, 1999, pages 367 - 376, XP001009714, ISSN: 0894-0282 *
WHITMAN S ET AL: "Selectable viruses and altered susceptibility mutants in Arabidopsis thaliana", PROC. NATL. ACAD. SCI. USA, vol. 96, January 1999 (1999-01-01), pages 772 - 77, XP002217536 *

Also Published As

Publication number Publication date
AU2003222893A1 (en) 2003-09-02
EP1472374A2 (fr) 2004-11-03
WO2003066900A2 (fr) 2003-08-14
US20050255455A1 (en) 2005-11-17
AU2003222893B2 (en) 2008-05-15
US7919677B2 (en) 2011-04-05
NZ534545A (en) 2008-06-30
MXPA04007718A (es) 2005-06-08
JP2006503544A (ja) 2006-02-02
FR2835698B1 (fr) 2004-08-06
FR2835698A1 (fr) 2003-08-15

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