WO2003066865A3 - Nukleinsäuremolekül zur regulation der genexpression in pflanzen - Google Patents

Nukleinsäuremolekül zur regulation der genexpression in pflanzen Download PDF

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Publication number
WO2003066865A3
WO2003066865A3 PCT/DE2003/000315 DE0300315W WO03066865A3 WO 2003066865 A3 WO2003066865 A3 WO 2003066865A3 DE 0300315 W DE0300315 W DE 0300315W WO 03066865 A3 WO03066865 A3 WO 03066865A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
plants
acid molecule
gene expression
sequence
Prior art date
Application number
PCT/DE2003/000315
Other languages
English (en)
French (fr)
Other versions
WO2003066865A2 (de
Inventor
Daguang Cai
Tim Thurau
Christian Jung
Original Assignee
Kws Saat Ag
Daguang Cai
Tim Thurau
Christian Jung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kws Saat Ag, Daguang Cai, Tim Thurau, Christian Jung filed Critical Kws Saat Ag
Priority to DE10390419T priority Critical patent/DE10390419D2/de
Priority to AU2003206654A priority patent/AU2003206654A1/en
Publication of WO2003066865A2 publication Critical patent/WO2003066865A2/de
Publication of WO2003066865A3 publication Critical patent/WO2003066865A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8239—Externally regulated expression systems pathogen inducible
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Nukleinsäuremolekül zur Regulation der Genexpression in Pflanzen, einen chimären Promotor, die Verwendung eines solchen Nukleinsäuremoleküls oder eines solchen Promotors zur pflanzlichen Pathogenabwehr sowie transgene Zellen und Pflanzen. Mit der vorliegenden Erfindung ist es erstmalig möglich, pflanzliche Abwehrreaktion gegen Nematoden lokal zu begrenzen. Bei dem erfindungsgemässen Nukleinsäuremolekül handelt es sich um ein Molekül, das; a. eine Sequenz gemäss SEQ ID NO. 1 aufweist oder; b. eine mit der Sequenz gemäss SEQ ID NO. 1 komplementäre Sequenz aufweist oder; c. ein Derivat der Sequenz gemäss a oder b aufweist oder; d. eine Sequenz aufweist, die mit einer der Sequenzen gemäss a bisc c hybridisiert, und das ein 5‘-Ende und ein 3‘-Ende besitzt, wobei stromaufwärts von dem 5‘-Ende keine weiteren regulatorischen Sequenzen vorhanden sind, die eine pathogeninduzierte Expression eines mit dem Nukleinsäuremolekül operativ verbundenen Gens derart beeinflussen, dass nach Pathogenbefall einer Pflanze neben einer lokalen Expression des Gens eine Genexpression auch in nicht vom Pathogenbefall unmittelbar betroffenen Gewebeteilen der Pflanze erfolgt.
PCT/DE2003/000315 2002-02-06 2003-02-05 Nukleinsäuremolekül zur regulation der genexpression in pflanzen WO2003066865A2 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE10390419T DE10390419D2 (de) 2002-02-06 2003-02-05 Nukleinsäuremolekül zur Regulation der Genexpression in Pflanzen
AU2003206654A AU2003206654A1 (en) 2002-02-06 2003-02-05 Nucleic acid molecule for regulation of gene expression in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10204910A DE10204910A1 (de) 2002-02-06 2002-02-06 Nukleinsäuremolekül zur Regulation der Genexpression in Pflanzen
DE10204910.6 2002-02-06

Publications (2)

Publication Number Publication Date
WO2003066865A2 WO2003066865A2 (de) 2003-08-14
WO2003066865A3 true WO2003066865A3 (de) 2003-11-20

Family

ID=27674568

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2003/000315 WO2003066865A2 (de) 2002-02-06 2003-02-05 Nukleinsäuremolekül zur regulation der genexpression in pflanzen

Country Status (3)

Country Link
AU (1) AU2003206654A1 (de)
DE (2) DE10204910A1 (de)
WO (1) WO2003066865A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2527450A1 (de) * 2008-12-11 2012-11-28 BASF Plant Science GmbH Pflanzenwurzelspezifische Nematodenresistenz

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998012335A1 (de) * 1996-09-18 1998-03-26 Dlo-Centrum Voor Plantenveredelings- En Reproductie Onderzoek (Cpro-Dlo) Nematodenresistenzgen

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998012335A1 (de) * 1996-09-18 1998-03-26 Dlo-Centrum Voor Plantenveredelings- En Reproductie Onderzoek (Cpro-Dlo) Nematodenresistenzgen

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BURROWS P R ET AL: "CELLULAR AND MOLECULAR APPROACHES TO THE CONTROL OF PLANT PARASITICNEMATODES", PLANT PARASITIC NEMATODES IN TEMPERATE AGRICULTURE, XX, XX, 1993, pages 609 - 630, XP002002337 *
CAI D ET AL: "POSITIONAL CLONING OF A GENE FOR NEMATODE RESISTANCE IN SUGAR BEET", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 275, 7 February 1997 (1997-02-07), pages 832 - 834, XP002055588, ISSN: 0036-8075 *
NITZ I ET AL: "PYK 10, A SEEDLING AND ROOT SPECIFIC GENE AND PROMOTER FROM ARABIDOPSIS THALIANA", PLANT SCIENCE, LIMERICK, IE, vol. 161, no. 2, 2001, pages 337 - 346, XP001013257, ISSN: 0168-9452 *

Also Published As

Publication number Publication date
WO2003066865A2 (de) 2003-08-14
DE10390419D2 (de) 2004-12-23
AU2003206654A1 (en) 2003-09-02
DE10204910A1 (de) 2004-03-04

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