WO2003046186A1 - siRNA EXPRESSION SYSTEM AND METHOD FOR PRODUCING FUNCTIONAL GENE KNOCK-DOWN CELLS USING THE SYSTEM - Google Patents
siRNA EXPRESSION SYSTEM AND METHOD FOR PRODUCING FUNCTIONAL GENE KNOCK-DOWN CELLS USING THE SYSTEM Download PDFInfo
- Publication number
- WO2003046186A1 WO2003046186A1 PCT/JP2002/012447 JP0212447W WO03046186A1 WO 2003046186 A1 WO2003046186 A1 WO 2003046186A1 JP 0212447 W JP0212447 W JP 0212447W WO 03046186 A1 WO03046186 A1 WO 03046186A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sirna expression
- rna
- functional gene
- gene knock
- producing functional
- Prior art date
Links
- 108020004459 Small interfering RNA Proteins 0.000 title abstract 2
- 238000003197 gene knockdown Methods 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 3
- 108020005544 Antisense RNA Proteins 0.000 abstract 2
- 230000000692 anti-sense effect Effects 0.000 abstract 2
- 239000003184 complementary RNA Substances 0.000 abstract 2
- 108020004999 messenger RNA Proteins 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 238000001727 in vivo Methods 0.000 abstract 1
- 230000002452 interceptive effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2002354121A AU2002354121A1 (en) | 2001-11-28 | 2002-11-28 | siRNA Expression System and Method for Producing Functional Gene Knockdown Cell Using the Same |
JP2003547618A JP3642573B2 (ja) | 2001-11-28 | 2002-11-28 | siRNA発現システムおよびこれを用いた機能遺伝子ノックダウン細胞等の生産方法 |
DE60237919T DE60237919D1 (de) | 2001-11-28 | 2002-11-28 | siRNA-EXPRESSIONSSYSTEM UND VERFAHREN ZUR HERSTELLUNG EINER ZELLE O. Ä. MIT KNOCKDOWN EINES FUNKTIONELLEN GENS UNTER VERWENDUNG DESSELBEN |
AT02785973T ATE483805T1 (de) | 2001-11-28 | 2002-11-28 | Sirna-expressionssystem und verfahren zur herstellung einer zelle o. ä. mit knockdown eines funktionellen gens unter verwendung desselben |
CA002468955A CA2468955A1 (en) | 2001-11-28 | 2002-11-28 | Sirna expression system and process for producing functional gene knockdown cell or the like using the same |
US10/470,197 US20050048647A1 (en) | 2001-11-28 | 2002-11-28 | Sirna expression system and process for producing functional gene knockdown cell or the like using the same |
KR10-2004-7008244A KR20040072643A (ko) | 2001-11-28 | 2002-11-28 | siRNA 발현시스템 및 이것을 이용한 기능유전자넉다운 세포 등의 생산방법 |
EP02785973A EP1462525B1 (en) | 2001-11-28 | 2002-11-28 | siRNA EXPRESSION SYSTEM AND PROCESS FOR PRODUCING FUNCTIONAL GENE KNOCKDOWN CELL OR THE LIKE USING THE SAME |
HK05109992.4A HK1078105A1 (en) | 2001-11-28 | 2005-11-09 | Sirna expression system and process for producing a cell which functional gene has been knocked down or the like using the same sirna |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2001-363385 | 2001-11-28 | ||
JP2001363385 | 2001-11-28 | ||
JPPCT/JP02-11293 | 2002-10-30 | ||
PCT/JP2002/011293 WO2003046173A1 (fr) | 2001-11-28 | 2002-10-30 | Systeme d'expression d'arn si et procede de production de cellules d'inactivation de genes fonctionnelles et analogues au moyen de ce systeme |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003046186A1 true WO2003046186A1 (en) | 2003-06-05 |
WO2003046186A8 WO2003046186A8 (fr) | 2005-09-29 |
Family
ID=26345149
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2002/012447 WO2003046186A1 (en) | 2001-11-28 | 2002-11-28 | siRNA EXPRESSION SYSTEM AND METHOD FOR PRODUCING FUNCTIONAL GENE KNOCK-DOWN CELLS USING THE SYSTEM |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1462525B1 (ja) |
JP (1) | JP3642573B2 (ja) |
AU (1) | AU2002354121A1 (ja) |
CA (1) | CA2468955A1 (ja) |
WO (1) | WO2003046186A1 (ja) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003091433A1 (fr) * | 2002-04-26 | 2003-11-06 | National Institute Of Advanced Industrial Science And Technology | Systeme d'expression pour molecule d'arn a tige-boucle ayant un effet d'arni |
WO2004022777A1 (en) * | 2002-09-04 | 2004-03-18 | Johnson & Johnson Research Pty Ltd | METHODS USING dsDNA TO MEDIATE RNA INTERFERENCE (RNAi) |
WO2005035778A1 (ja) * | 2003-10-09 | 2005-04-21 | Kyowa Hakko Kogyo Co., Ltd. | α1,6-フコシルトランスフェラーゼの機能を抑制するRNAを用いた抗体組成物の製造法 |
WO2005056792A1 (ja) * | 2003-12-12 | 2005-06-23 | National Institute Of Advanced Industrial Science And Technology | インターフェロン応答が軽減された長い干渉用二重鎖rna |
EP1545624A2 (en) * | 2001-12-27 | 2005-06-29 | ALLELE BIOTECHNOLOGY & PHARMACEUTICALS, INC. | Compositions for dna mediated gene silencing |
WO2005068630A1 (ja) * | 2003-12-16 | 2005-07-28 | National Institute Of Advanced Industrial Science And Technology | 干渉用二重鎖rna |
JP2005533484A (ja) * | 2002-01-22 | 2005-11-10 | コールド スプリング ハーバー ラボラトリー | Rna干渉のための方法及び組成物 |
EP1613722A4 (en) * | 2002-10-18 | 2006-08-02 | Genta Salus Llc | OLIGONUCLEOTIDES INHIBITORS BASED ON RNA |
JP2007515966A (ja) * | 2003-12-23 | 2007-06-21 | ザ・トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルバニア | 疾患の併用療法のための組成物および方法 |
JP2007527244A (ja) * | 2004-03-05 | 2007-09-27 | ベニテック インコーポレイテッド | RNAi作用媒介物の同時デリバリーのためのマルチプロモーター発現カセット |
JPWO2005017154A1 (ja) * | 2003-08-18 | 2007-11-01 | 財団法人ヒューマンサイエンス振興財団 | 改良されたsiRNA分子およびこれを用いた遺伝子発現の抑制法 |
WO2010071206A1 (ja) * | 2008-12-18 | 2010-06-24 | 国立大学法人名古屋大学 | siRNA転写/発現用DNAコンストラクト及びその利用 |
US7893036B2 (en) | 2001-07-12 | 2011-02-22 | University Of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
US10233247B2 (en) | 1999-04-09 | 2019-03-19 | Kyowa Hakko Kirin Co., Ltd | Method of modulating the activity of functional immune molecules |
US10233475B2 (en) | 2000-10-06 | 2019-03-19 | Kyowa Hakko Kirin Co., Ltd | Antibody composition-producing cell |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8202846B2 (en) | 2000-03-16 | 2012-06-19 | Cold Spring Harbor Laboratory | Methods and compositions for RNA interference |
CA2403397A1 (en) | 2000-03-16 | 2001-09-20 | Genetica, Inc. | Methods and compositions for rna interference |
US9994853B2 (en) | 2001-05-18 | 2018-06-12 | Sirna Therapeutics, Inc. | Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference |
ES2312753T5 (es) | 2002-02-14 | 2012-12-13 | City Of Hope | Procedimientos para producir moléculas de ARN de interferencia en células de mamífero y usos terapéuticos para tales moléculas |
JP2005517450A (ja) * | 2002-02-20 | 2005-06-16 | サーナ・セラピューティクス・インコーポレイテッド | 短干渉核酸(siNA)を用いるRNA干渉媒介性標的発見および標的評価 |
US20050059044A1 (en) * | 2003-06-03 | 2005-03-17 | Graham Michael Wayne | Double-stranded nucleic acid |
US20080207539A1 (en) * | 2003-09-01 | 2008-08-28 | Patrick Arbuthnot | Self-Processing Rna Expression Cassette |
US10508277B2 (en) | 2004-05-24 | 2019-12-17 | Sirna Therapeutics, Inc. | Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference |
FR2877674A1 (fr) * | 2004-11-08 | 2006-05-12 | Genoxay Sa | Animaux modeles comprenant au moins un transgene et une sequence permettant l'expression controlee d'un rna interferant avec ledit transgene |
EP1681347A1 (en) * | 2005-01-18 | 2006-07-19 | Metanomics GmbH & Co. KGaA | Improved methods for double-stranded RNA mediated gene silencing |
WO2006116756A1 (en) * | 2005-04-28 | 2006-11-02 | Benitec, Limited. | Multiple-rnai expression cassettes for simultaneous delivery of rnai agents related to heterozygotic expression patterns |
US20100299771A1 (en) * | 2007-09-17 | 2010-11-25 | Helmholtz Zentrum Munchen- Deutsches Forschungzentrum Fur Gesundheit Und Umwelt(Gmbh) | MEANS AND METHODS FOR shRNA MEDIATED CONDITIONAL KNOCKDOWN OF GENES |
Citations (4)
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WO2001034815A1 (en) | 1999-11-05 | 2001-05-17 | Cambria Biosciences, Llc | Dsrna as insect control agent |
WO2001068836A2 (en) | 2000-03-16 | 2001-09-20 | Genetica, Inc. | Methods and compositions for rna interference |
WO2002066638A1 (fr) * | 2001-02-22 | 2002-08-29 | Gencom Corporation | Gene recombinant contenant une sequence de repetition inversee et utilisation correspondante |
WO2002068635A2 (en) | 2001-02-28 | 2002-09-06 | Novartis Forschungsstiftung Zweigniederlassung Friedrich Miescher Institute For Biomedical Research | Methods of inhibiting expression of a target gene in mammalian cells |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
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US20030198627A1 (en) * | 2001-09-01 | 2003-10-23 | Gert-Jan Arts | siRNA knockout assay method and constructs |
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2002
- 2002-11-28 JP JP2003547618A patent/JP3642573B2/ja not_active Expired - Fee Related
- 2002-11-28 WO PCT/JP2002/012447 patent/WO2003046186A1/ja active Application Filing
- 2002-11-28 AU AU2002354121A patent/AU2002354121A1/en not_active Abandoned
- 2002-11-28 CA CA002468955A patent/CA2468955A1/en not_active Abandoned
- 2002-11-28 EP EP02785973A patent/EP1462525B1/en not_active Expired - Lifetime
Patent Citations (5)
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WO2001034815A1 (en) | 1999-11-05 | 2001-05-17 | Cambria Biosciences, Llc | Dsrna as insect control agent |
WO2001068836A2 (en) | 2000-03-16 | 2001-09-20 | Genetica, Inc. | Methods and compositions for rna interference |
WO2002066638A1 (fr) * | 2001-02-22 | 2002-08-29 | Gencom Corporation | Gene recombinant contenant une sequence de repetition inversee et utilisation correspondante |
EP1371727A1 (en) | 2001-02-22 | 2003-12-17 | Gencom Corporation | Recombinant gene containing inverted repeat sequence and utilization thereof |
WO2002068635A2 (en) | 2001-02-28 | 2002-09-06 | Novartis Forschungsstiftung Zweigniederlassung Friedrich Miescher Institute For Biomedical Research | Methods of inhibiting expression of a target gene in mammalian cells |
Non-Patent Citations (19)
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Cited By (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10233247B2 (en) | 1999-04-09 | 2019-03-19 | Kyowa Hakko Kirin Co., Ltd | Method of modulating the activity of functional immune molecules |
US10233475B2 (en) | 2000-10-06 | 2019-03-19 | Kyowa Hakko Kirin Co., Ltd | Antibody composition-producing cell |
US9850487B2 (en) | 2001-07-12 | 2017-12-26 | University Of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
US8232260B2 (en) | 2001-07-12 | 2012-07-31 | University Of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
US10731155B2 (en) | 2001-07-12 | 2020-08-04 | University Of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
US7893036B2 (en) | 2001-07-12 | 2011-02-22 | University Of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
US8530438B2 (en) | 2001-07-12 | 2013-09-10 | University Of Massachusetts | Vivo production of small interfering RNAs that mediate gene silencing |
US8557785B2 (en) | 2001-07-12 | 2013-10-15 | University Of Massachusetts | In vivo production of small interfering RNAS that mediate gene silencing |
US9175287B2 (en) | 2001-07-12 | 2015-11-03 | University Of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
EP1545624A2 (en) * | 2001-12-27 | 2005-06-29 | ALLELE BIOTECHNOLOGY & PHARMACEUTICALS, INC. | Compositions for dna mediated gene silencing |
EP1545624A4 (en) * | 2001-12-27 | 2006-03-29 | Allele Biotechnology & Pharmac | COMPOSITIONS FOR DNA-MEDIATED GEN SILENCING |
US7294504B1 (en) | 2001-12-27 | 2007-11-13 | Allele Biotechnology & Pharmaceuticals, Inc. | Methods and compositions for DNA mediated gene silencing |
US7422896B1 (en) | 2001-12-27 | 2008-09-09 | Allele Biotechnology & Pharmaceuticals, Inc. | Compositions for DNA mediated gene silencing |
US7625750B2 (en) | 2001-12-27 | 2009-12-01 | Allele Biotechnology & Pharmaceuticals, Inc. | Compositions for DNA mediated gene silencing |
JP2005533484A (ja) * | 2002-01-22 | 2005-11-10 | コールド スプリング ハーバー ラボラトリー | Rna干渉のための方法及び組成物 |
JP4758067B2 (ja) * | 2002-01-22 | 2011-08-24 | コールド スプリング ハーバー ラボラトリー | Rna干渉のための方法及び組成物 |
JP2011135882A (ja) * | 2002-01-22 | 2011-07-14 | Cold Spring Harbor Lab | Rna干渉のための方法及び組成物 |
WO2003091433A1 (fr) * | 2002-04-26 | 2003-11-06 | National Institute Of Advanced Industrial Science And Technology | Systeme d'expression pour molecule d'arn a tige-boucle ayant un effet d'arni |
WO2004022777A1 (en) * | 2002-09-04 | 2004-03-18 | Johnson & Johnson Research Pty Ltd | METHODS USING dsDNA TO MEDIATE RNA INTERFERENCE (RNAi) |
EP1613722A4 (en) * | 2002-10-18 | 2006-08-02 | Genta Salus Llc | OLIGONUCLEOTIDES INHIBITORS BASED ON RNA |
JP4572299B2 (ja) * | 2003-08-18 | 2010-11-04 | 財団法人ヒューマンサイエンス振興財団 | 改良されたsiRNA分子およびこれを用いた遺伝子発現の抑制法 |
JPWO2005017154A1 (ja) * | 2003-08-18 | 2007-11-01 | 財団法人ヒューマンサイエンス振興財団 | 改良されたsiRNA分子およびこれを用いた遺伝子発現の抑制法 |
CN1867672B (zh) * | 2003-08-18 | 2012-11-14 | 财团法人日本健康科学振兴财团 | 改良的siRNA分子和应用该改良的siRNA分子的抑制基因表达的方法 |
WO2005035778A1 (ja) * | 2003-10-09 | 2005-04-21 | Kyowa Hakko Kogyo Co., Ltd. | α1,6-フコシルトランスフェラーゼの機能を抑制するRNAを用いた抗体組成物の製造法 |
JPWO2005035778A1 (ja) * | 2003-10-09 | 2006-12-21 | 協和醗酵工業株式会社 | α1,6−フコシルトランスフェラーゼの機能を抑制するRNAを用いた抗体組成物の製造法 |
WO2005056792A1 (ja) * | 2003-12-12 | 2005-06-23 | National Institute Of Advanced Industrial Science And Technology | インターフェロン応答が軽減された長い干渉用二重鎖rna |
JP2011087601A (ja) * | 2003-12-16 | 2011-05-06 | National Institute Of Advanced Industrial Science & Technology | 干渉用二重鎖rna |
WO2005068630A1 (ja) * | 2003-12-16 | 2005-07-28 | National Institute Of Advanced Industrial Science And Technology | 干渉用二重鎖rna |
JPWO2005068630A1 (ja) * | 2003-12-16 | 2007-07-26 | 独立行政法人産業技術総合研究所 | 干渉用二重鎖rna |
JP2007515966A (ja) * | 2003-12-23 | 2007-06-21 | ザ・トラスティーズ・オブ・ザ・ユニバーシティ・オブ・ペンシルバニア | 疾患の併用療法のための組成物および方法 |
JP2007527244A (ja) * | 2004-03-05 | 2007-09-27 | ベニテック インコーポレイテッド | RNAi作用媒介物の同時デリバリーのためのマルチプロモーター発現カセット |
JP4763681B2 (ja) * | 2004-03-05 | 2011-08-31 | ベニテック インコーポレイテッド | RNAi作用媒介物の同時デリバリーのためのマルチプロモーター発現カセット |
JPWO2010071206A1 (ja) * | 2008-12-18 | 2012-05-31 | 国立大学法人名古屋大学 | siRNA転写/発現用DNAコンストラクト及びその利用 |
WO2010071206A1 (ja) * | 2008-12-18 | 2010-06-24 | 国立大学法人名古屋大学 | siRNA転写/発現用DNAコンストラクト及びその利用 |
Also Published As
Publication number | Publication date |
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EP1462525A4 (en) | 2007-02-07 |
JP3642573B2 (ja) | 2005-04-27 |
WO2003046186A8 (fr) | 2005-09-29 |
JPWO2003046186A1 (ja) | 2005-04-07 |
CA2468955A1 (en) | 2003-06-05 |
AU2002354121A1 (en) | 2003-06-10 |
EP1462525B1 (en) | 2010-10-06 |
EP1462525A1 (en) | 2004-09-29 |
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