WO2003033702A1 - METHOD OF DETECTING AND QUANTIFYING HEMOLYSIN-PRODUCING BACTERIA BY OVERWHELMINGLY DETECTING AND QUANTIFYING THERMOSTABLE HEMOLYSIN-RELATED GENES (tdh-RELATED HEMOLYSIN GENES) OF FOOD POISONING BACTERIA - Google Patents

METHOD OF DETECTING AND QUANTIFYING HEMOLYSIN-PRODUCING BACTERIA BY OVERWHELMINGLY DETECTING AND QUANTIFYING THERMOSTABLE HEMOLYSIN-RELATED GENES (tdh-RELATED HEMOLYSIN GENES) OF FOOD POISONING BACTERIA Download PDF

Info

Publication number
WO2003033702A1
WO2003033702A1 PCT/JP2002/010795 JP0210795W WO03033702A1 WO 2003033702 A1 WO2003033702 A1 WO 2003033702A1 JP 0210795 W JP0210795 W JP 0210795W WO 03033702 A1 WO03033702 A1 WO 03033702A1
Authority
WO
WIPO (PCT)
Prior art keywords
hemolysin
tdh
genes
quantifying
detecting
Prior art date
Application number
PCT/JP2002/010795
Other languages
English (en)
French (fr)
Inventor
Takeshi Koizumi
Satoshi Yamamoto
Takeshi Itoh
Hiroshi Nakagawa
Original Assignee
Nichirei Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nichirei Corporation filed Critical Nichirei Corporation
Priority to EP02801592A priority Critical patent/EP1445313A4/en
Priority to US10/492,942 priority patent/US20050239067A1/en
Priority to KR10-2004-7005760A priority patent/KR20040045871A/ko
Publication of WO2003033702A1 publication Critical patent/WO2003033702A1/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
PCT/JP2002/010795 2001-10-18 2002-10-17 METHOD OF DETECTING AND QUANTIFYING HEMOLYSIN-PRODUCING BACTERIA BY OVERWHELMINGLY DETECTING AND QUANTIFYING THERMOSTABLE HEMOLYSIN-RELATED GENES (tdh-RELATED HEMOLYSIN GENES) OF FOOD POISONING BACTERIA WO2003033702A1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02801592A EP1445313A4 (en) 2001-10-18 2002-10-17 PROCESS FOR DETECTING AND QUANTIFYING HEMOLYSIN-PRODUCING BACTERIA BY MEANS OF IDENTIFICATION AND QUANTIFICATION MOST OF GENES (Tdh-RELATED HEMOLYSIN GENES) RELATED TO TEMPERATURE-STABLE HEMOLYSIN) FOOD-POISONING CAUSING BACTERIA
US10/492,942 US20050239067A1 (en) 2001-10-18 2002-10-17 Method of detecting and quantifying hemolysin-producing bacteria by overwhelmingly detecting and quantifying thermostable hemolysin-related genes (tdh-related hemolysin genes) of food poisoning bacteria
KR10-2004-7005760A KR20040045871A (ko) 2001-10-18 2002-10-17 식중독세균이 보유하는 내열성 용혈독소 관련유전자(ｔｄｈ 관련 독소 유전자)를 총체적으로검출·정량함으로써 용혈독소 생산균을 검출·정량하는 방법

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001/320403 2001-10-18
JP2001320403A JP2003116579A (ja) 2001-10-18 2001-10-18 食中毒細菌の有する耐熱性溶血毒素関連遺伝子（ｔｄｈ関連毒素遺伝子）を網羅的に検出・定量することによる溶血毒素産生細菌の検出・定量方法

Publications (1)

Publication Number Publication Date
WO2003033702A1 true WO2003033702A1 (en) 2003-04-24

Family

ID=19137803

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/010795 WO2003033702A1 (en) 2001-10-18 2002-10-17 METHOD OF DETECTING AND QUANTIFYING HEMOLYSIN-PRODUCING BACTERIA BY OVERWHELMINGLY DETECTING AND QUANTIFYING THERMOSTABLE HEMOLYSIN-RELATED GENES (tdh-RELATED HEMOLYSIN GENES) OF FOOD POISONING BACTERIA

Country Status (6)

Country Link
US (1) US20050239067A1 (ja)
EP (1) EP1445313A4 (ja)
JP (1) JP2003116579A (ja)
KR (1) KR20040045871A (ja)
CN (1) CN1606622A (ja)
WO (1) WO2003033702A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7495094B2 (en) 2003-09-05 2009-02-24 Tosoh Corporation Detection reagent for thermostable direct hemolysin-related hemolysin gene of Vibrio parahaemolyticus
CN111518922A (zh) * 2019-09-02 2020-08-11 广东美格基因科技有限公司 用于检测产毒副溶血性弧菌的荧光定量pcr方法及相应的试剂盒

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100659157B1 (ko) * 2005-03-09 2006-12-19 이규호 콜레라세균, 비브리오장염세균 및 비브리오패혈증세균의 특이적 탐지 방법
FR2982367B1 (fr) * 2011-11-08 2013-12-27 Biomerieux Sa Procede de detection de l'hemolysine delta de staphylococcus aureus par spectrometrie de masse directement a partir d'une population bacterienne
WO2015190106A1 (ja) * 2014-06-11 2015-12-17 東洋製罐グループホールディングス株式会社 食中毒菌検出用担体、食中毒菌検出用キット、食中毒菌の検出方法、及び食中毒菌用ｐｃｒ反応液
CN110836975B (zh) * 2019-11-29 2023-05-02 扬州大学 一种检测副溶血性弧菌td毒素的胶体金塑封检测卡及其制备方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04293486A (ja) * 1991-03-25 1992-10-19 Shimadzu Corp 細菌検出のためのオリゴヌクレオチドおよびそれを用いた検出法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04293486A (ja) * 1991-03-25 1992-10-19 Shimadzu Corp 細菌検出のためのオリゴヌクレオチドおよびそれを用いた検出法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BEJ A.K. ET AL.: "Detection of total and hemolysin-producing vibrio parahaemolyticus in shellfish using multiplex PCR amplification to tl, tdh and trh", J. MICROBIOL. METHODS, vol. 36, 1999, pages 215 - 225, XP002909008 *
See also references of EP1445313A4 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7495094B2 (en) 2003-09-05 2009-02-24 Tosoh Corporation Detection reagent for thermostable direct hemolysin-related hemolysin gene of Vibrio parahaemolyticus
CN111518922A (zh) * 2019-09-02 2020-08-11 广东美格基因科技有限公司 用于检测产毒副溶血性弧菌的荧光定量pcr方法及相应的试剂盒

Also Published As

Publication number Publication date
KR20040045871A (ko) 2004-06-02
JP2003116579A (ja) 2003-04-22
US20050239067A1 (en) 2005-10-27
EP1445313A1 (en) 2004-08-11
EP1445313A4 (en) 2005-01-26
CN1606622A (zh) 2005-04-13

Similar Documents

Publication Publication Date Title
Skovhus et al. Real-time quantitative PCR for assessment of abundance of Pseudoalteromonas species in marine samples
Teixeira et al. Enterococcus
AU2003241311A1 (en) Simple catalytic dna biosensors for ions based on color changes
WO2006043178A3 (en) Enzymes
WO2008125365A8 (en) Microbial population analysis
Warner et al. Randomly amplified polymorphic DNA analysis of starved and viable but nonculturable Vibrio vulnificus cells
WO2004058990A3 (en) Genetic polymorphisms associated with stenosis, methods of detection and uses thereof
EP2357478A3 (en) Diagnosis of disease
WO2005047474A3 (en) Nucleic acid detection method having increased sensitivity
TW200700725A (en) Detection of nucleic acid associated with disease
WO2004020654A3 (en) Solid phase based nucleic acid assays combining high affinity and high specificity
WO2003033702A1 (en) METHOD OF DETECTING AND QUANTIFYING HEMOLYSIN-PRODUCING BACTERIA BY OVERWHELMINGLY DETECTING AND QUANTIFYING THERMOSTABLE HEMOLYSIN-RELATED GENES (tdh-RELATED HEMOLYSIN GENES) OF FOOD POISONING BACTERIA
WO2001066721A3 (en) Nucleic acid sensor molecules
WO2008104680A3 (fr) Milieu de détection et/ou d'identification de bactéries
Leng et al. Genome sequencing of cold‐adapted Planococcus bacterium isolated from traditional shrimp paste and protease identification
Sogge et al. Gene flow, recombination, and selection in cyanobacteria: population structure of geographically related Planktothrix freshwater strains
WO2004063392A3 (es) Medio de cultivo selectivo para el aislamiento y deteccion de especies del genero streptococcus
WO2001036683A3 (en) Method for rapid and accurate identification of microorganisms
WO2004057016A3 (en) METHODS OF CAPTURING, DETECTING AND QUANTIFYING RNA:DNA HYBRIDS AND A MODIFIED RNase H USEFUL THEREIN
WO2004046375A3 (en) Method for the detection of microorganisms in pharmaceutical products
WO2003014704A3 (en) Rapid and specific detection of campylobacter
WO2003034025A3 (en) Methods and reagents for improved cell-based assays
MX2008000283A (es) Ensayos y metodos de extraccion mejorados para enzimas alimenticias.
WO2003033694A1 (fr) Amorces et methode de detection de bacteries
AU4397101A (en) Method and kit for the detection of schistosomiasis through the polymerase chainreaction

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): CN ID IN KR PH SG US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FR GB GR IE IT LU MC NL PT SE SK TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 10492942

Country of ref document: US

Ref document number: 1-2004-500555

Country of ref document: PH

Ref document number: 1020047005760

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: 1200400424

Country of ref document: VN

WWE Wipo information: entry into national phase

Ref document number: 2002801592

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 1094/CHENP/2004

Country of ref document: IN

WWE Wipo information: entry into national phase

Ref document number: 20028255658

Country of ref document: CN

WWP Wipo information: published in national office

Ref document number: 2002801592

Country of ref document: EP