WO2003027648A3 - Fluorometre compact a phase et a longueurs d'onde multiples - Google Patents

Fluorometre compact a phase et a longueurs d'onde multiples Download PDF

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Publication number
WO2003027648A3
WO2003027648A3 PCT/US2002/030926 US0230926W WO03027648A3 WO 2003027648 A3 WO2003027648 A3 WO 2003027648A3 US 0230926 W US0230926 W US 0230926W WO 03027648 A3 WO03027648 A3 WO 03027648A3
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WO
WIPO (PCT)
Prior art keywords
fluorometer
compact
phase
phase fluorometer
multiwavelength
Prior art date
Application number
PCT/US2002/030926
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English (en)
Other versions
WO2003027648A2 (fr
Inventor
Salvador M Fernandez
Ernest F Guignon
Louis Ernest St
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Ciencia Inc
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Publication date
Application filed by Ciencia Inc filed Critical Ciencia Inc
Priority to EP02773645A priority Critical patent/EP1436596A2/fr
Publication of WO2003027648A2 publication Critical patent/WO2003027648A2/fr
Publication of WO2003027648A3 publication Critical patent/WO2003027648A3/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G01N2021/6471—Special filters, filter wheel

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un instrument compact pouvant fonctionner comme un fluorimètre en régime permanent ou comme un fluoromètre à phase, et pouvant par conséquent mesurer l'intensité de fluorescence en régime permanent et la durée de vie de la fluorescence à partir d'un échantillon de fluorescence. La source lumineuse fournit quatre longueurs d'ondes d'excitation générées par des diodes électroluminescentes (DEL) et pouvant être sélectionnées par l'utilisateur, et comprend un élément d'auto-étalonnage ainsi qu'un moyen permettant de compenser des erreurs de mesure de phase causées par la diaphonie phase-amplitude dans les appareils électroniques de détection.
PCT/US2002/030926 2001-09-28 2002-09-27 Fluorometre compact a phase et a longueurs d'onde multiples WO2003027648A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP02773645A EP1436596A2 (fr) 2001-09-28 2002-09-27 Fluorometre compact a phase et a longueurs d'onde multiples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32590901P 2001-09-28 2001-09-28
US60/325,909 2001-09-28

Publications (2)

Publication Number Publication Date
WO2003027648A2 WO2003027648A2 (fr) 2003-04-03
WO2003027648A3 true WO2003027648A3 (fr) 2004-01-29

Family

ID=23269982

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/030926 WO2003027648A2 (fr) 2001-09-28 2002-09-27 Fluorometre compact a phase et a longueurs d'onde multiples

Country Status (3)

Country Link
US (1) US20030062485A1 (fr)
EP (1) EP1436596A2 (fr)
WO (1) WO2003027648A2 (fr)

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GB0416732D0 (en) * 2004-07-27 2004-09-01 Precisense As A method and apparatus for measuring the phase shift induced in a light signal by a sample
US6970241B1 (en) 2004-08-24 2005-11-29 Desa Richard J Device for enabling slow and direct measurement of fluorescence polarization
FR2877092B1 (fr) * 2004-10-26 2006-12-29 Commissariat Energie Atomique Methode de mesure relative du rendement quantique de fluorescence de colorants en solution
US7576334B2 (en) * 2007-03-19 2009-08-18 The Regents Of The University Of Michigan Photoacoustic indicators
WO2009056669A1 (fr) * 2007-10-31 2009-05-07 Wallac Oy Système de mesure d'échantillons
CN102159937A (zh) * 2008-09-19 2011-08-17 三井造船株式会社 采用强度调制激光的荧光检测装置和荧光检测方法
CN102150035A (zh) * 2008-09-19 2011-08-10 三井造船株式会社 采用强度调制激光的荧光检测装置和荧光检测方法
WO2011020983A2 (fr) * 2009-06-30 2011-02-24 Marshalls Of Cambridge Aerospace Limited Mesure d'une concentration dans un fluide
WO2013040058A1 (fr) * 2011-09-12 2013-03-21 Jian Guo Imagerie par durée de vie de fluorescence ou de luminescence
US9140648B2 (en) 2013-03-12 2015-09-22 Ecolab Usa Inc. Fluorometer with multiple detection channels
US9383312B2 (en) 2013-11-06 2016-07-05 Ciencia, Inc. Electro-optic grating-coupled surface plasmon resonance (EOSPR)
WO2016034775A1 (fr) * 2014-09-05 2016-03-10 Thermo Fisher Scientific Oy Procédé et appareil de mesure optique d'échantillon de liquide
WO2016100701A1 (fr) * 2014-12-17 2016-06-23 Singulex, Inc. Analyseur multiplexé de molécule unique
JP2018105761A (ja) * 2016-12-27 2018-07-05 東亜ディーケーケー株式会社 多波長蛍光分析装置
JP2018146539A (ja) * 2017-03-09 2018-09-20 東亜ディーケーケー株式会社 多波長蛍光分析装置
CN111247419A (zh) * 2017-09-13 2020-06-05 (株)吉普朗 具有增强检测能力的传感器
CN109406454B (zh) * 2018-09-11 2021-01-15 江苏大学 一种改进的z扫描装置
US20210389249A1 (en) * 2018-10-25 2021-12-16 Hamamatsu Photonics K.K. Optical measurement device and optical measurement method
CN109211789A (zh) * 2018-10-31 2019-01-15 大连依利特分析仪器有限公司 荧光检测用紫外led自动切换装置
US11783917B2 (en) 2019-03-21 2023-10-10 Illumina, Inc. Artificial intelligence-based base calling
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
US11423306B2 (en) * 2019-05-16 2022-08-23 Illumina, Inc. Systems and devices for characterization and performance analysis of pixel-based sequencing
IL295560A (en) 2020-02-20 2022-10-01 Illumina Inc An artificial intelligence-based many-to-many base reader
TWI735371B (zh) 2020-11-04 2021-08-01 致茂電子股份有限公司 螢光檢測系統
CN114441487B (zh) * 2020-11-04 2023-11-03 致茂电子(苏州)有限公司 荧光检测系统
FR3119678A1 (fr) * 2021-02-05 2022-08-12 Siaap Sonde optique pour analyse par fluorescence en milieux aqueux de molécules organiques presentant au moins un fluorophore et procédé de mise en œuvre

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US4840485A (en) * 1986-12-17 1989-06-20 I.S.S. (U.S.A.) Inc. Frequency domain cross-correlation fluorometry with phase-locked loop frequency synthesizers
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EP0732582A2 (fr) * 1995-03-16 1996-09-18 Bio-Rad Laboratories, Inc. Excitation accordable et/ou représenter par une image ou moyen fluorescente d'émission
EP0981043A1 (fr) * 1998-08-18 2000-02-23 Molecular Devices Corporation Système optique amélioré pour un fluoromètre à balayage

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Publication number Publication date
WO2003027648A2 (fr) 2003-04-03
EP1436596A2 (fr) 2004-07-14
US20030062485A1 (en) 2003-04-03

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