WO2003014362A3 - Verfahren zur herstellung eines marker-freien mutierten zielorganismus sowie dafür geeignete plasmidvektoren - Google Patents

Verfahren zur herstellung eines marker-freien mutierten zielorganismus sowie dafür geeignete plasmidvektoren Download PDF

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Publication number
WO2003014362A3
WO2003014362A3 PCT/EP2002/008231 EP0208231W WO03014362A3 WO 2003014362 A3 WO2003014362 A3 WO 2003014362A3 EP 0208231 W EP0208231 W EP 0208231W WO 03014362 A3 WO03014362 A3 WO 03014362A3
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WO
WIPO (PCT)
Prior art keywords
target organism
marker
producing
free
same
Prior art date
Application number
PCT/EP2002/008231
Other languages
English (en)
French (fr)
Other versions
WO2003014362A2 (de
Inventor
Markus Pompejus
Corinna Klopprogge
Oskar Zelder
Wolfgang Liebl
Original Assignee
Basf Ag
Markus Pompejus
Corinna Klopprogge
Oskar Zelder
Wolfgang Liebl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag, Markus Pompejus, Corinna Klopprogge, Oskar Zelder, Wolfgang Liebl filed Critical Basf Ag
Priority to JP2003519491A priority Critical patent/JP2004538003A/ja
Priority to CA002456222A priority patent/CA2456222A1/en
Priority to KR10-2004-7001776A priority patent/KR20040020080A/ko
Priority to US10/486,125 priority patent/US20040171160A1/en
Priority to EP02794516A priority patent/EP1417317A2/de
Publication of WO2003014362A2 publication Critical patent/WO2003014362A2/de
Publication of WO2003014362A3 publication Critical patent/WO2003014362A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft einen Plasmidvektor, der in einem Zielorganismus nicht repliziert, enthaltend folgende Komponenten: a) einen Replikationsursprung (origin of replication) für einen Wirtsorganismus, der nicht mit dem Zielorganismus identisch ist, b) mindestens einen genetischen Marker, c) optional einen Sequenzabschnitt, der den Transfer von DNA durch Konjugation ermöglicht (mob-Sequenz), d) einen Sequenzabschnitt, der homolog zu Sequenzen des Zielorganismus ist und im Zielorganismus homologe Rekombination ermöglicht, e) einem Gen für eine Galaktose Kinase unter der Kontrolle eines Promotors.
PCT/EP2002/008231 2001-08-06 2002-07-24 Verfahren zur herstellung eines marker-freien mutierten zielorganismus sowie dafür geeignete plasmidvektoren WO2003014362A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2003519491A JP2004538003A (ja) 2001-08-06 2002-07-24 マーカーを含まない変異型標的生物の調製及びそのために好適なプラスミドベクター
CA002456222A CA2456222A1 (en) 2001-08-06 2002-07-24 Method for producing a marker-free mutated target organism and plasmid vectors suitable for the same
KR10-2004-7001776A KR20040020080A (ko) 2001-08-06 2002-07-24 마커-프리 돌연변이화 표적 생물체를 제조하는 방법 및이를 위해 적합한 플라스미드 벡터
US10/486,125 US20040171160A1 (en) 2001-08-06 2002-07-24 Method for producing a marker-free mutated target organism and plasmid vectors suitable for the same
EP02794516A EP1417317A2 (de) 2001-08-06 2002-07-24 Verfahren zur herstellung eines marker-freien mutierten zielorganismus sowie dafür geeignete plasmidvektoren

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10137815.7 2001-08-06
DE10137815A DE10137815A1 (de) 2001-08-06 2001-08-06 Verfahren zur Herstellung eines marker-freien mutierten Zielorganismus sowie dafür geeignete Plasmidvektoren

Publications (2)

Publication Number Publication Date
WO2003014362A2 WO2003014362A2 (de) 2003-02-20
WO2003014362A3 true WO2003014362A3 (de) 2003-05-01

Family

ID=7694077

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/008231 WO2003014362A2 (de) 2001-08-06 2002-07-24 Verfahren zur herstellung eines marker-freien mutierten zielorganismus sowie dafür geeignete plasmidvektoren

Country Status (7)

Country Link
US (1) US20040171160A1 (de)
EP (1) EP1417317A2 (de)
JP (1) JP2004538003A (de)
KR (1) KR20040020080A (de)
CA (1) CA2456222A1 (de)
DE (1) DE10137815A1 (de)
WO (1) WO2003014362A2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102005032429A1 (de) 2005-01-19 2006-07-20 Degussa Ag Allele des mqo-Gens aus coryneformen Bakterien
DE102005013676A1 (de) 2005-03-24 2006-09-28 Degussa Ag Allele des zwf-Gens aus coryneformen Bakterien
DE102005023829A1 (de) 2005-05-24 2006-11-30 Degussa Ag Allele des opcA-Gens aus coryneformen Bakterien
DE102006032634A1 (de) 2006-07-13 2008-01-17 Evonik Degussa Gmbh Verfahren zur Herstellung von L-Aminosäuren
DE102008001874A1 (de) 2008-05-20 2009-11-26 Evonik Degussa Gmbh Verfahren zur Herstellung von L-Aminosäuren
ES2855992T3 (es) * 2015-12-11 2021-09-27 Wacker Chemie Ag Cepa de microorganismos y procedimiento para la producción fermentativa exenta de antibióticos de sustancias y proteínas de bajo peso molecular
EP3415622A1 (de) 2017-06-14 2018-12-19 Evonik Degussa GmbH Verfahren zur herstellung von feinchemikalien mittels eines corynebakteriums, welches modifizierte alpha-1,6-glucosidasen sezerniert
EP3456833A1 (de) 2017-09-18 2019-03-20 Evonik Degussa GmbH Methode zur fermentativen production von l-aminosäuren
RU2019128538A (ru) 2018-09-26 2021-03-11 Эвоник Оперейшенс ГмбХ Способ ферментативного получения l-лизина

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0752477A2 (de) * 1990-01-16 1997-01-08 Baylor College Of Medicine Expressionsvektoren die Steroidrezeptoren produzieren, Steroidrezeptoren-Chimären, Screeningtests für Steroidrezeptoren und Verwendung der so hergestellten Rezeptoren und Rezeptor-Vektoren in klinischen Analysen
WO2000009705A2 (en) * 1998-08-11 2000-02-24 Danisco A/S Selection method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3841453A1 (de) * 1988-12-09 1990-06-13 Degussa Verfahren zum konjugativen transfer von mobilisierbaren vektoren aus e.coli in gram-positive bakterien und dafuer geeignete vektoren

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0752477A2 (de) * 1990-01-16 1997-01-08 Baylor College Of Medicine Expressionsvektoren die Steroidrezeptoren produzieren, Steroidrezeptoren-Chimären, Screeningtests für Steroidrezeptoren und Verwendung der so hergestellten Rezeptoren und Rezeptor-Vektoren in klinischen Analysen
WO2000009705A2 (en) * 1998-08-11 2000-02-24 Danisco A/S Selection method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GRAY F. CROUSE ET AL.: "Gene engineering by selectable intraplasmid recombination: construction of novel dihydrofolate reductase minigenes", GENE, vol. 84, 1989, pages 165 - 172, XP002228041 *

Also Published As

Publication number Publication date
US20040171160A1 (en) 2004-09-02
WO2003014362A2 (de) 2003-02-20
JP2004538003A (ja) 2004-12-24
KR20040020080A (ko) 2004-03-06
EP1417317A2 (de) 2004-05-12
DE10137815A1 (de) 2003-02-27
CA2456222A1 (en) 2003-02-20

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