Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
  • A61P31/20—Antivirals for DNA viruses
  • A61P31/22—Antivirals for DNA viruses for herpes viruses
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P37/00—Drugs for immunological or allergic disorders
  • A61P37/02—Immunomodulators
  • A61P37/04—Immunostimulants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K39/00—Medicinal preparations containing antigens or antibodies
  • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
  • A61K2039/515—Animal cells
  • A61K2039/5154—Antigen presenting cells [APCs], e.g. dendritic cells or macrophages
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K39/00—Medicinal preparations containing antigens or antibodies
  • A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
  • C12N2710/00011—Details
  • C12N2710/16011—Herpesviridae
  • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
  • C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Definitions

• polypeptide includes proteins, fragments of proteins, and peptides, whether isolated from natural sources, produced by recombinant techniques or chemicaUy synthesized. Polypeptides of the invention typicaUy comprise at least about 6 amino acids, and preferably at least about 15 amino acids.
• HSV polypeptide includes HSV-1 and HSV-2, unless otherwise indicated. References to amino acids of HSV proteins or polypeptides are based on the genomic sequence information regarding HSV-2 as described in A. Dolan et al., 1998, J. Virol. 72(3) :2010-2021. As noted below, the predicted polypeptide sequence of ICPO of HSV-2 based on sequencing RNA from ceUs transfected with a fragment of ICPO differs from the pubkshed sequence by the omission of amino acid Q26.
• compositions comprising such carriers are formulated by weU known conventional methods (see, for example, Remington's Pharmaceutical Sciences, 18th edition, A. Gennaro, ed., Mack PubHshing Co., Easton, PA, 1990).
• a carrier may bear the agents or polypeptides in a variety of ways, including covalent bonding either direcdy or via a Hnker group.
• Suitable carriers include proteins such as albumins (e.g., U.S. Patent No. 4,507,234, to Kato et al), peptides and polysaccharides such as arninodextran (e.g., U.S. Patent No. 4,699,784, to Shih et al.).
• a carrier may also bear an agent by noncovalent bonding or by encapsulation, such as within a Hposome vesicle (e.g., U.S. Patent Nos. 4,429,008 and 4,873,088).
• the invention provides polynucleotides that encode one or more polypeptides of the invention.
• the complete genome sequence of HSV-2, strain HG52, can be found on the NCBI web site (www.ncbi.ttih.gov), Accession No. Z86099.
• the polynucleotide can be included in a vector.
• the vector can further comprise an expression control sequence operably linked to the polynucleotide of the invention.
• the vector includes one or more polynucleotides encoding other molecules of interest.
• the polynucleotide of the invention and an additional polynucleotide can be linked so as to encode a fusion protein.
• Another preferred adjuvant is a saponin, preferably QS21, which may be used alone or in combination with other adjuvants.
• an enhanced system involves the combination of a monophosphoryl Hpid A and saponin derivative, such as the combination of QS21 and 3D-MPL as described in WO 94/00153, or a less reactogenic composition where the QS21 is quenched with cholesterol, as described in WO 96/33739.
• Other preferred formulations comprises an oH-in-water emulsion and tocopherol.
• a particularly potent adjuvant formulation involving QS21, 3D-MPL and tocopherol in an oU-in-water emulsion is described Hi WO 95/17210.
• Another adjuvant that may be used is AS-2 (SnHti -KHne Beecham). Any vaccine provided herein may be prepared using weU known methods that result in a combination of antigen, immune response enhancer and a suitable carrier or excipient.
• the HSV-2 D ⁇ A present in IRV DX32 therefore includes U L 48, U L 49, U L 50, and most likely the intervenHig U L 49.5. Since TCC 4.2E1 and 2.3 react with RSI G31 and DX32, but not with RP2 ( Figure IB), recognition of U L 49, U L 49.5, or U L 50 is most likely.
• T-ceU antigens were confirmed by targeted subcloning and overlapping peptides.
• the 262 bp Sma I fragment of U L 49 of HSV-2 encoding amino acids 105-190 was subcloned into pUEX3 to yield plasmid 49.262.12.
• This protein stimulated TCC 4.2E1 (Table 2).
• DNA fragments encoding U L 50 118-312 and 118-250 were subcloned into pUEX3. Fusion proteins expressing these fragments were active (Table 2).
• polypeptides expressed as C-terminal fusion to VP22 can be co-transported into ceUs
• expression of proteins as VP22 fusions may be of interest as a type of adjuvant preparation. This can be tested by expression of heterologous epitopes in VP22.
• VP16 and VP22 of HSV-1 are strongly, noncovalentiy associated in infected ceUs as shown by coimmunoprecipitation. These proteins co-locaHze in the perinuclear area of ceUs (G.
• HSV-specific T ceUs including CD8+ ceUs, can be detected in cervical lymphocytes.
• the high EC50 value ( ⁇ 1 ⁇ M) may be due to the carboxy- terminus taU predicted to he outside the peptide-binding groove and reduce binding to HLA B*4501.
• Vaccinia-ICPO from B. Rouse Manickan E et al., J. Vkol. 1995, 69:4711-16 was grown and titered (KoeUe DM et al, J. Vkol. 1994, 68:2803-10).
• Clone RW51 specificaUy lysed vac-ICPO targets ( Figure 12). The avaUabihty of the vaccinia was fortuitous, and not requked to confkm the result of expression cloning.

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• Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
• Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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• 2002
  • 2002-07-31 AT AT02748276T patent/ATE488249T1/de not_active IP Right Cessation
  • 2002-07-31 AU AU2002317604A patent/AU2002317604A1/en not_active Abandoned
  • 2002-07-31 WO PCT/US2002/024306 patent/WO2003011893A2/en not_active Ceased
  • 2002-07-31 US US10/210,428 patent/US6814969B2/en not_active Expired - Fee Related
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• 2004
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