"COMPOSITIONS FOR ORNAMENTAL PLANTS."
This invention relates to new compositions for ornamental plants. This invention also refers to a procedure for preparing the compositions, and their use as fertilisers and protectors for ornamental plants in pots and gardens.
State of the art.-
For their correct growth and development, all plants need a series of essential nutritive elements that are usually found naturally in the soil. Generally, the nutrients provided by the substrate, especially for plants grown in pots for ornamental use, are insufficient to guarantee the plant's correct development throughout its life cycle. The use of additional fertilisers thus becomes necessary.
We find conventional fertilisers in liquid form or as granules, and most of them contain inorganic or organic nitrogen that does not come exclusively from proteins.
Nitrogenated materials derived from vegetable or animal proteins have also been used as fertilisers. For example, patent US 4491464 describes a liquid fertiliser consisting of a plant protein hydrolysate, and patent application JP
62084007 describes a plant growth promoter based on hydrolysates of animal origin presented in liquid form.
For solid formulations of protein hydrolysates, we refer to patent application EP 440339, which describes nitrogenated granules as fertilisers, obtained from animal residues hydrolysed in a basic medium.
However, to date, no fertiliser or protector compositions have been described comprising protein hydrolysates in tablet form.
There is therefore a need to find an alternative to the known solid fertilisers. The present invention provides novel products that have fertilising and protective properties, are slowly released, not exposed to the environment, with a nitrogenated content coming exclusively from protein hydrolysis and with a final formulation that is specific for the part of the plant for which it is meant.
Disclosure of the invention.-
The present invention provides new compositions for ornamental plants, compacted in tablet form, and comprising a hydrolysate of porcine mucosa proteins, potassium and phosphorus, with a free amino acid content superior to
6%.
In a preferred embodiment of the invention, the hydrolysate of porcine mucosa proteins is obtained by proteolytic enzymatic hydrolysis, and is a mixture of free amino acids and peptides with a mean molecular weight of less than 600 daltons.
In a more preferred embodiment, the free amino acids of the protein hydrolysate are present in at least 60% of the total protein, and the peptides of the hydrolysate have a molecular weight of less than 2,000 daltons.
More preferred are compositions containing from 1.5% to 6% by weight of total nitrogen, which comes entirely from protein hydrolysate, from 1.5% to 10% by weight of P O5 and from 0.5% to 7% by weight of K2O.
Especially preferred are compositions compacted in tablet form that also contain active oxygen as oxygen supply, favouring the development of the root and soil micro-organisms in stressful situations for the plant, comprising, by weight, approximately 7% of free amino acids, 5% of active oxygen, 2% of total nitrogen, 2% of P2O5 and 1% of K2O.
Also especially preferred are compositions compacted in tablet form that also contain Ca, Fe and B micro-elements, to favour flowering, comprising, by weight, approximately 18% of free amino acids, 0.1% of Ca, 0.3% of Fe, 0.05% of B, 5% of total nitrogen, 10% of P O5 and 6% of K2O.
Also especially preferred are compositions compacted in tablet form that also contain vitamins Bι, B2 and B3 and the Ca, Mg, Fe, Mn and Zn microelements, for leaf growth and development, comprising, by weight, approximately 18% of free amino acids, 0.1% of vitamin B^ 0.1% of vitamin B2, 0.1% of vitamin B3, 0.1% of Ca, 0.1% of Mg, 0.3% of Fe, 0.2% of Mn, 0.2% of Zn, 5% of total nitrogen, 5% of P2O5 and 5% of K2O.
Also especially preferred are compositions compacted in tablet form that also contain vitamins B , B2, C, E and D3 and the Fe, Mn, Zn, B and Mo microelements, to facilitate the uptake of nutrients from the soil through the root, comprising, by weight, approximately 10% of free amino acids, 0.1% of vitamin B
0.1% of vitamin B2, 0.1% of vitamin C, 0.03% of vitamin E, 2000 IU of vitamin D3, 0.3% of Fe, 0.2% of Mn, 0.2% of Zn, 0.05% of B, 0.02% of Mo, 3% of total nitrogen, 8% of P2O5 and 5% of K2O.
The present invention also refers to a procedure for preparing the compositions, comprising of mixing the product that comes from the hydrolysate of
porcine mucosa proteins with potassium and phosphorus salts and, depending on each case, with a suitable salt required to provide the active oxygen, or with a salt of the suitable micro-elements, and when necessary, with the suitable vitamins, compacting said compositions in tablet form. The hydrolysate of porcine mucosa proteins can be prepared by enzymatic digestion of porcine intestinal mucosa, using a proteolytic enzyme. The enzyme is then disactivated at a temperature of, for example, 90°C. It is then decanted for, for example, 3 hours, maintaining the temperature at, for example, 90°C. The aqueous phase is then filtered, complexed and filtered again, obtaining a liquid to which hydrogen peroxide is added to remove sulphites. Later a thermal concentration is carried out at a temperature of, for example, 100°C. A preservative, for example potassium sorbate, is added to the concentrated product. The protein hydrolysate obtained is then subjected to atomisation, thus obtaining the product called PalbioR 450 (manufactured by Bioiberica, S.A.). Another aspect of this invention is the utility of the compositions compacted in tablet form of the present invention as fertilisers for ornamental plants in pots and gardens.
Yet another aspect of the present invention is the utility of the compositions compacted in tablet form of the present invention as protectors against diseases, nutritional disorders and stressful conditions in ornamental plants in pots and gardens.
To use the compositions described in this invention, they are compacted in tablet form, using conventional techniques and excipients as described by Remington: The Science and Practice of Pharmacy, 20th Edition, Lippincott Williams & Wilkins, USA.
The new compositions of the invention compacted in tablet form are buried in the soil, preventing them from being exposed to air, preferably 2-3 cm beneath the surface.
The dosage will depend on the size of the pot, for example for 0.5-1.5 litre plant pots, 2-3 tablets can be used at a 2-3 month interval of application.
For plants grown in larger plant pots or gardens, a larger number of tablets will be used.
One advantage of this invention, in comparison with other fertilisers known in the art, lies in the fact that, since they have a high amino acid content, the plant finds it easier to overcome the usual stressful conditions in cultivated plants,
especially associated with transplantation, disease and when there is a lack of light or water, changes of temperature and bad weather conditions.
Another advantage of the present invention, in comparison with the liquid fertilisers known in the art, lies in the fact that the tablets have a long-lasting effect, whereas liquid fertilisers have an immediate effect and are easily leached out.
Another advantage of this invention, in comparison with the solid fertiliser granules known in the art, lies in the fact that, when the tablets are placed in the soil, the risk of environmental pollution is reduced to a minimum, and their intake by pets and young children is prevented. Another advantage is that, unlike granules, for a sustained release of the tablets, they do not have to be coated with resins.
Another advantage of the present invention is that the tablets can contain a bitter component (sucrose octa-acetate) as a safety measure to prevent them from being accidentally swallowed by pets and children.
Detailed description of the invention.-
The following examples are not exclusive, and they illustrate the preparation of the compositions compacted in tablet form described in this invention. In the fertiliser industry, the quantity of nitrogen, phosphorus and potassium is usually given as a percentage of N (nitrogen), percentage of P2O5 (phosphorus pentoxide) and percentage of K2O (potassium oxide), even though these elements are not present in this form.
The tablets were prepared by direct compression in controlled humidity conditions, after the sieving and the progressive mixing of the different components.
Nomenclature used in the Examples:
CAB-O-SIL M5: Silicon dioxide
AVICEL PH 200: Microcrystalline cellulose RED IRON OXIDE: E-172 iron oxide
YELLOW IRON OXIDE: E-172 hydrated iron oxide
GREEN FC LACQEUR: Mixture of aluminium lacquers E-102 Tartrazine and E-
133 Bright blue FCF
BROWN AZ LACQEUR: Mixture of aluminium lacquers E-102 Tartrazine, E-122 Red and E-132 Indigotine
DABQUEL Na2Ca: Calcium disodium salt of ethylendiaminotetracetic acid, dihydrate
DABQUEL Mg P: Magnesium disodium salt of ethylendiaminotetracetic acid, dihydrate DABEERSEN NaFe: Ferric sodium salt of ethylendiaminotetracetic acid
DABQUELAN Mn P: Manganese disodium salt of ethylendiaminotetracetic acid DABQUELAN Zn P: Zinc disodium salt of ethylendiaminotetracetic acid Example 1 : Tablets to facilitate the development of the root and the soil microorganisms under stress conditions for the plant PalbioR 450 20.000 g
Monopotassium phosphate 4.000 g
Sodium percarbonate 40.000 g
Polyvinylpyrrolidone 5.000 g
Cab-O-Sil M5 1.000 g Avicel PH 200 27.100 g
Magnesium stearate 2.000 g
Yellow iron oxide 0.500 g
Sucrose octa-acetate 0.400 q
100 g which corresponds to the composition in claim 6, with the following weight percentage:
Free amino acids 7%
Active oxygen 5%
Total nitrogen 2% P2O5 2%
K2O 1%
The entire procedure was performed under controlled humidity conditions
(30%).
The sodium percarbonate and the Avicel PH 200 were sieved, separately, through a 1 mm mesh.
The Rulon mixer performed a 5-minute pre-mix of monopotassium phosphate, polyvinylpyrrolidone, yellow iron oxide, sucrose octa-acetate and 1 g of
Cab-O-Sil M5. The pre-mix was then sieved through a 0.500 mm mesh. The sieved product was again introduced into the mixer, which was run for 10 minutes to prepare what is called PREMIX I.
A 5-minute premix of PalbioR 450 with 99 g of Cab-O-Sil M5 was prepared in the Rulon mixer. It was then sieved through a 0.800 mm mesh. The product was introduced into the mixer again and run for 10 minutes, making what is called PREMIX II. PREMIX I and PREMIX II and the sieved sodium percarbonate and Avicel
PH 200 were introduced into a "V" type mixer with a 50 L capacity, and mixed for 20 minutes. The magnesium stearate was then sieved through a 0.400 mm mesh, and added to the "V" mixer, mixing for 3 minutes.
The mixture was stored in hermetically closed containers with a double plastic bag and silica gel between both bags, until compression.
The compression was performed in a machine equipped with oblong grooved 21x9 mm punches. We obtained tablets of 1.000+5% g, with a mean hardness of 190 N and friability inferior to 0.1%. Example 2: Tablets to favour flowering The tablet forming method described in Example 1 was followed.
PalbioR 450 50.000 g
Monopotassium phosphate 19.300 g
Boric acid 0.300 g
Dabquel Na2Ca 1.030 g Dabeersen NaFe 2.310 g
Cab-O-Sil M5 2.000 g
Avicel PH 200 21.660 g
Magnesium stearate 2.000 g
Red iron oxide 1.000 g Sucrose octa-acetate 0.400 g
100 g which corresponds to the composition in claim 8, with the following weight percentage:
Free amino acids 18% Ca 0.1%
Fe 0.3%
B 0.05%
Total nitrogen 5%
P2O5 10%
The tablet forming method described in Example 1 was followed, but in this case PREMIX I consisted of: boric acid, Dabquel Na2Ca, Dabeersen NaFe, red iron oxide, sucrose octa-acetates, 2 g of Cab-O-Sil M5 and 166 g of Avicel PH 200. PREMIX II consisted of: PalbioR 450, 198 g of Cab-O-Sil M5 and monopotassium phosphate. In this case sodium percarbonate was not introduced into the "V" mixer. Example 3: Tablets for leaf growth and development
PalbioR 450 50.000 g
Monopotassium phosphate 9.700 g Potassium sulphate 3.400 g
Dabeersen NaFe 2.310 g
Dabquelan Mn P 1.540 g
Dabquelan Zn P 1.330 g
Dabquel Na2Ca 1.030 g Dabquel Mg P 1.670 g
Thiamin (vitamin B^ 0.100 g
Riboflavin (vitamin B2) 0.100 g
Nicotinamide (vitamin B3) 0.100 g
Cab-O-Sil M5 2.000 g Avicel PH 200 22.820 g
Magnesium stearate 2.000 g
Green FC Lacquer 1.500 g
Sucrose octa-acetate 0.400 g
100 g which corresponds to the composition in claim 10, with the following weight percentage:
Free amino acids 18%
Vitamin B, 0.1%
Vitamin B2 0.1% Vitamin B3 0.1%
Ca 0.1%
Mg 0.1%
Fe 0.3%
Mn 0.2% Zn 0.2%
Total nitrogen 5%
P2O5 5%
K2O 5%
The entire procedure was performed in controlled humidity conditions
(30%).
The potassium sulphate and the Avicel PH 200 were sieved separately through a 1 mm mesh.
A 5-minute premix of thiamin, riboflavin, nicotinamide, sucrose octa-acetate and 2 g de Cab-O-Sil M5 was prepared in the Rulon mixer. The pre-mix was then sieved through a 0.500 mm mesh. The sieved product was introduced again into the mixer, adding 160 g of Avicel PH 200, and run for 10 minutes, obtaining what is called PREMIX I.
A 5-minute premix of Dabeersen NaFe, Dabquelan Mn P, Dabquelan Zn P,
Dabquel Na2Ca, Dabquel Mg P and Green FC lacquer was made in the Rulon mixer. It was then sieved and introduced into the mixer again, adding potassium sulphate and 522 g of Avicel PH 200, mixing for another 10 minutes, producing what is called PREMIX II.
The PREMIX II was added to the PREMIX I and mixed for 10 minutes, producing what is called VITAMINERAL PREMIX. A 5-minute premix of PalbioR 450 with 198 g of Cab-O-Sil M5 was prepared in the Rulon mixer. It was then sieved together with monopotassium phosphate using a 0.800 mm mesh. The sieved product was introduced into the mixer for 10 minutes, producing what is called PREMIX III.
The VITAMINERAL PREMIX, PREMIX III and 1600 g of Avicel PH 200 were introduced into the "V" mixer with a 50 I capacity, and mixed for 20 minutes.
The magnesium stearate sieved through a 0.400 mm mesh was added and mixed for 3 minutes.
The procedure described in Example 1 was then followed. Example 4: Tablets to facilitate the uptake of nutrients from the soil through the root
PalbioR 450 30.000 g
Monopotassium phosphate 15.400 g
Boric acid 0.300 g
Dabeersen NaFe 2.310 g Dabquelan Mn P 1.540 g
Dabquelan Zn P 1.330 g
Ammonium molibdate 0.040 g
Thiamin (vitamin B-t) 0.100 g
Riboflavin (vitamin B2) 0.100 g Coated vitamin C 0.100 g
Tocopherol acetate 33% powder 0.100 g
Vitamin D3 100,000 lU/g 0.020 g
Cab-O-Sil M5 1.200 g
Avicel PH 200 43.560 g Magnesium stearate 2.000 g
Brown AZ Lacquer 1.500 g
Sucrose octa-acetate 0.400 g
100 g which corresponds to the composition in claim 12, with the following weight percentage:
Free amino acids 10%
Vitamin B 0.1%
Vitamin B2 0.1%
Vitamin C 0.1% Vitamin E 0.03%
Vitamin D3 2,000 IU
Fe 0.3%
Mn 0.2%
Zn 0.2% B 0.05%
Mo 0.02%
Total nitrogen 3%
P2O5 8%
K2O 5% The tablet forming method described in Example 3 was followed, but in this case the Avicel PH 200 was sieved. The PREMIX I consisted of: thiamin, riboflavin, vitamin C, vitamin E, vitamin D, sucrose octa-acetate and 2 g of Cab-O- Sil M5. The PREMIX II consisted of: Dabeersen NaFe, Dabquelan Zn P, Dabquelan Mn P, ammonium molibdate, boric acid, Brown AZ lacquer and 1196 g of Avicel PH 200. The PREMIX III consisted of: PalbioR 450, 118 g of Cab-O-Sil
M5 and monopotassium phosphate. The VITAMINERAL PREMIX, the PREMIX III and 3000 g of Avicel PH 200 were introduced into the "V" mixer, and the procedure in Example 3 was followed up to the end. Phytotoxicity test A phytotoxicity test was performed using the following plant material:
Spider plant (Chlorophytum comosum), Dieffenbachia (Dieffenbachia sp.), Lettuce {Lactuca sativa), Kalanchoe (Kalanchoe blossfeldiana), Fern (Nephrolepis exaltata), Pothos (Epipremmum aureum), Pansy (Impatiens sp.), Brazilian Carnival (Anthirrinum majus), Chinese Carnation (Dianthus sp.), Rubber plant (Ficus elastica "Robusta') and Dracena (Dracaena marginata and Dracaena deremensis).
0.5 L and 1 L containers were used.
In general, phytotoxicity symptoms due to over dosage were found, as expected in most vegetal nutrition products, when a dose higher than that recommended was applied to plants of such size:
6 tablets / plant in 0.5 L containers 14 tablets / plant in 1 L containers Taking this into account, it can be affirmed that none of the previously-mentioned compositions are phytotoxic at the recommended doses. All those compositions can thus be safely used for the purposes stated in the present invention.
Safety of the product
To ensure the safety of the compositions of the present invention towards accidental ingestion by the users, a toxicity study has been carried out in rats. The results confirm the safety of the compositions: the LD50 is superior to 2500 mg/kg, when taken orally.
Agronomic efficacy of the compositions 1. Trial on geranium
Pelargoniums {Pelargonium zonale) were used as a flower species representing outdoor plants, which were grown in 13 cm diameter plant pots containing an organic substrate based on sphagne peat. The experiment was run outdoors in the months of July and August in an area covered with weed-proof plastic on the Mediterranean coast close to Barcelona. The tablets from Examples 1 and 2 were tested separately, at a dose to meet the nutritive requirements of the species, established at 3 tablets per plant pot, buried in the substrate. The trial included control plants with no type of fertiliser. All the plants were watered daily
with enough water to completely dampen the substrate and they were kept outdoors for 45 days, after which the efficacy assessments described in Table 1 were performed. Variables were chosen to quantify the improvement obtained with the treatments in relation to the growth and development of the plant, together with its decorative and ornamental quality.
2. Trial on Spatiphyllum Spatiphyllum sp. was used as a species representing indoor plants, grown in 13 cm diameter plant pots, with sphagne peat as a substrate. The experiment was conducted in a growth chamber under controlled weather conditions (temperature: 20°C day/18°C night; relative humidity: 70-90%; photoperiod: 12h day/12h night). The tablets from Example 3 were tested, at a dose to meet the nutritive requirements of this species, consisting of 3 tablets per plant pot, duly buried in the substrate. Control plants that received no fertilisation were also included. All the plants were watered with 300 mL of water, twice a week. 45 days after the start of the experiment, the measurements included in Table 2 were taken, corresponding to the variables that are most representative of the development of the plant and its ornamental quality.
TABLE 2
3. Trial on petunia
Petunia was used (Petunia x hybrida cv Dreams Red) as a seasonal plant, grown in 17 cm diameter plant pots, with peat as the substrate. The experiment was conducted in a glass greenhouse close to Barcelona, in the months of February and March. The tablets from Example 2 were tested, at a dosage fit for the type of plant and experimental conditions, at 4 tablets per plant pot, duly buried in the substrate. The performance of the tablets from Example 2 was compared with a control treatment, consisting of non-fertilised plants; and plants subject to conventional fertiliser treatment, supplied with the same quantity of nutritive elements as in Example 2, but without amino acids, which are a characteristic of this invention. The plants were automatically watered several times a week, depending on the ambient conditions, with a somewhat short quantity of water to provoke water stress and show the efficacy of the amino acids in the example by increasing the plant's tolerability to adverse conditions. After 70 days, the measurements shown on Table 3 were taken, the parameters of the Table indicate the development of the plant and its ornamental capacity.
TABLE 3
4. Trial on New Guinea Impatiens
The same procedure was followed as in the trial on the petunia, in the same conditions of water stress, but in this case New Guinea Impatiens (Impatiens hawkeri) were used, and assessed the tablets from Example 3. The efficacy measurements shown in Table 4 were taken 45 days after starting the experiment. On this occasion, conventional fertilisation consisted of providing the same quantity of nutritive elements as in Example 3, but without amino acids and vitamins.
TABLE 4
5. Remarks
As can be seen in Tables 1 , 2, 3 and 4, in general, the tablets described in this invention, characterised in that they all contain amino acids from a hydrolysate of porcine mucosa proteins, significantly favour the growth of the plant (fresh weight aerial part, leaf area, height and breadth of the plant), and they also improve its ornamental appearance (leaf coverage, chlorophyll and number of flowers), both in normal growth conditions and in stressful situations.
With geranium (table 1 ), the tablets from Examples 1 and 2 produced an increase of biomass (in weight, leaf surface and plant breadth) of up to 69 % and increased the height of the plant by over 13% in just one and a half months. They also improved the green colour of the leaves (more chlorophyll) and increased the number of flowers.
With Spatiphyllum (table 2), the tablets from Examples 3 and 4 similarly increased the biomass (fresh weight, leaf area and leaf coverage) by up to 41% and height by 11%. The chlorophyll content also increased noticeably (up to 15%) making the leaves greener and indicating good general plant condition.
With Petunia and New Guinea Impatiens (tables 3 and 4), under low
availability conditions, the improvements obtained with the tablets from Examples 2 and 3 were even more evident (up to 200% of increase of some variables) and were also superior in all cases to the results obtained with conventional fertilisation. To summarise, all the above-mentioned agronomic efficacy trials performed with the tablets from the Examples described in this invention confirm that these new compositions for ornamental plants produce very noticeable improvements in the development and ornamental appearance of the plants, which justify their agronomic use. These improvements are superior to those obtained using conventional fertilisation and they also maintain the plant in a better state of development when conditions are not adequate, for example when water is scarce.