WO2002101386A1 - Outil analytique - Google Patents

Outil analytique Download PDF

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Publication number
WO2002101386A1
WO2002101386A1 PCT/JP2002/005602 JP0205602W WO02101386A1 WO 2002101386 A1 WO2002101386 A1 WO 2002101386A1 JP 0205602 W JP0205602 W JP 0205602W WO 02101386 A1 WO02101386 A1 WO 02101386A1
Authority
WO
WIPO (PCT)
Prior art keywords
odor
sample
component
activated carbon
analytical
Prior art date
Application number
PCT/JP2002/005602
Other languages
English (en)
Japanese (ja)
Inventor
Takahiro Taya
Masako Aki
Original Assignee
International Reagents Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by International Reagents Corporation filed Critical International Reagents Corporation
Priority to JP2003504093A priority Critical patent/JPWO2002101386A1/ja
Publication of WO2002101386A1 publication Critical patent/WO2002101386A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/525—Multi-layer analytical elements

Definitions

  • the present invention is used for detection and measurement of components in a biological sample, particularly components in excrement, and is used as a so-called clinical laboratory diagnostic agent.
  • a stool sample suspension diluted in an excess of a buffer solution is added, and developed on a support such as a nitrocellulose membrane to check for the presence or absence of a reaction.
  • a sample liquid absorbing member is provided at an end different from the sample addition site.
  • the absorbent member a member having good water absorbability, such as a filter paper or an accumulation of cellulose fibers, is usually used. Issues to be solved
  • An object of the present invention is to detect and measure components contained in a biological sample, particularly microbial components in feces by the immunochromatographic method, and to measure odor components such as fecal samples.
  • a member that absorbs odorous components is installed in the analytical instrument to improve the working environment and the disposal environment
  • the goal is to provide better analytical tools.
  • in order to immunologically detect the virus that causes diarrhea from diarrheal feces collected from diarrhea patients it is necessary to measure about 1 to 30% of the fecal suspension as a sample. In such a case, the odor of feces leaks out of the analytical instrument during the analysis, deteriorating the working environment, and furthermore, the storage of the used analytical instrument for disposal and subsequent disposal. Odor may cause disposal environment to deteriorate. In order not to deteriorate the working environment and the disposal environment, it was necessary to control the odor in the analytical instrument. Disclosure of the invention
  • a sample liquid absorbing member containing a component that absorbs odor due to a sample is installed in an analytical tool for measuring a component contained in a test sample.
  • the inventors have found that the odor can be controlled more in the analytical instrument, and completed the present invention.
  • the configuration of the present invention is as follows.
  • An analysis tool for measuring a component contained in a test sample wherein a sample liquid absorbing member containing a component for controlling odor caused by the sample is provided in the sample for analysis. Analytical tools.
  • sample liquid absorbing member containing a component for controlling odor is a member containing activated carbon.
  • Fig. 1 shows a schematic diagram of an immunochromatographic analysis tool
  • Fig. 2 shows a schematic diagram of a flow-through immunochromatography tool.
  • the meanings of the symbols are as follows.
  • the installation of a sample liquid absorbing member containing a component for controlling odor in the analytical tool of the present invention means a device for analyzing and measuring a target substance present in a sample emitting odor.
  • a typical example of a sample that emits an odor is feces, but is not limited thereto, and rot, urine, pus, and the like are also suitably targeted. In particular, it is suitable for analyzing fecal samples.
  • the immunochromatography method is exemplified as a representative example, and the test strip method, the chemical test method, the immunological test method (agglutination method, EIA method) Etc.) is not to use the means of the present invention. It is valid.
  • controlling the odor generally means absorbing the odor component, but is not limited to this, and causes a chemical reaction with the odor component to cause the decomposition or the odor source.
  • Means such as trapping chains and inhibiting bacterial growth can also be used effectively alone or in combination.
  • known absorbents, decomposing enzymes, antibacterial agents and the like can be used.
  • Fig. 1 shows a schematic diagram of a general tool for analyzing a gas inlet mat graph.
  • the odor component absorbing component (deodorizing component) is contained in the sample liquid absorbing member of member 6.
  • activated carbon is mixed and kneaded with cellulose powder as a base material.
  • the ratio of the activated carbon to be mixed is preferably from 0.01 to 1 lg per 1 g of cellulose, more preferably from 0.1 to 0.5 g.
  • the origin of the activated carbon is not particularly limited, but a powdery one is advantageous in the step of mixing with activated carbon.
  • the substrate into which activated carbon is mixed is not particularly limited as long as it absorbs the sample liquid sufficiently, such as a cellulose powder or a bundle of cellulose fibers.
  • Analysis tools are stored in an appropriate case It may be provided as a tool, and in general, a material formed of a moisture-impermeable material having an opening at the sample addition site 1 and the measurement site 5 is used.
  • the material is preferably plastic, and the support member 3, the absorbing member 6, and the like are molded.
  • the support member is formed by forming a thin layer such as a membrane filter on which a membrane filter is formed.
  • the capture antibody or antigen is immobilized on this membrane (2).
  • the sample containing the antigen is added to (1), the solution flows through the pores of the membrane and reacts with the antibody labeled with gold colloid, colored latex, etc. to obtain an antigen-antibody reaction product. .
  • the antigen-antibody reaction product further flows through the pores of the membrane, is trapped in (5), and is colored to indicate the presence of the antigen.
  • the case for storing the analysis tool usually has a structure in which the sample liquid absorbing part 6 does not have an opening.However, the sample liquid absorbing part 6 is more open to the outside than such a closed type case. Providing the mouth makes it difficult for backflow to occur due to the large evaporation of the sample liquid, which makes the reaction signal clear even after standing for a long time. Can be detected. In such a case, particularly, since the sample liquid absorbing portion 6 is open to the outside, the odor of the fecal sample is easily leaked to the outside.
  • the use of the method of the present invention prevents the odor from leaking to the outside even with the analytical tool stored in the case where the sample liquid absorbing portion 6 is provided with an opening, thus improving the working environment. Preservation of used analytical tools and improvement of disposal environment.
  • Example 1 the present invention will be described specifically with reference to Examples, but the present invention is not limited to the following Examples.
  • Example 1
  • a sample liquid absorbing member made of cellulose powder mixed with activated carbon was used as a member of the sample liquid absorbing section 6 of the immunochromatographic tool as shown in Fig. 1.
  • an analysis tool equipped with an existing sample liquid absorbing member that did not contain activated carbon was used to conduct a virus test for the cause of diarrhea in human diarrhea stool. The odor was judged. As a result, those who mixed activated carbon were all judged to be odor negative. On the other hand, all existing products were judged to have odor. In the sensory test on the feeling of use, significant usability was confirmed. In addition, when disposing of used analytical instruments, sensory tests were conducted on unpleasant odors during their storage and disposal, but a significant improvement in the disposal environment was confirmed.
  • Figure 2 shows the flow-through type analysis tool.
  • the lower absorbent member uses an absorbent member made of cellulose powder mixed with activated carbon. You.
  • the analytical tool according to the present invention showed that all of the 10 test subjects showed the superiority of the working environment and the disposal environment over the existing tool. It could be confirmed.
  • Example 4
  • the reaction was allowed to proceed overnight at 2-8 ° C. After washing three times with the same buffer, further immersing in the same buffer containing 0.1% BSA, and then washing twice with the same buffer, the concentration of latex particles becomes 0.1%.
  • the buffer was prepared as described above.
  • a glass fiber tip (Whatman) was used.
  • a membrane filter made by Nitrocellulose (Millipore) was used.
  • Antibodies adjusted to 100 ⁇ g / mL with PBS buffer are applied to the reaction detection sites using a dotting device (manufactured by BioDot), dried, and placed in PBS buffer containing 0.1% BSA at room temperature. After immersion for 1 hour at room temperature, dry at room temperature did.
  • a dotting device manufactured by BioDot
  • Example 1 The product created in Example 1 was used.
  • the members (1) to (4) were cut to a size suitable for the immunochromatography tool, and the immunochromatography tool shown in Fig. 1 was created. Operation
  • a sample with a strong odor such as a fecal sample is analyzed by an analysis tool such as immunochromatography
  • the odor is absorbed into the analysis tool.
  • the odor does not leak out and the effect of improving the working environment and disposal environment can be expected.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Outil analytique pouvant absorber un ingrédient odorant dérivé d'un échantillon pour améliorer l'atmosphère de travail ou de relaxation. L'outil comprend un organe simple absorbant les odeurs contenant du charbon actif. L'organe absorbant absorbe ainsi les ingrédients odorants dérivés de l'échantillon, ce qui permet d'améliorer l'atmosphère de travail ou de relaxation.
PCT/JP2002/005602 2001-06-07 2002-06-06 Outil analytique WO2002101386A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2003504093A JPWO2002101386A1 (ja) 2001-06-07 2002-06-06 分析用具

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001172328 2001-06-07
JP2001-172328 2001-06-07

Publications (1)

Publication Number Publication Date
WO2002101386A1 true WO2002101386A1 (fr) 2002-12-19

Family

ID=19013968

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/005602 WO2002101386A1 (fr) 2001-06-07 2002-06-06 Outil analytique

Country Status (2)

Country Link
JP (1) JPWO2002101386A1 (fr)
WO (1) WO2002101386A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006101137A1 (fr) * 2005-03-22 2006-09-28 Kobayashi Pharmaceutical Co., Ltd. Appareil pour déterminer le stade de ménopause

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10237798A (ja) * 1996-12-12 1998-09-08 Elf Antar Fr 汚染液体用濾紙
JPH1114512A (ja) * 1997-06-20 1999-01-22 Tomakomai Rinshiyou Kensa Center:Kk 消臭採便容器、糞便臭抑制方法および消臭剤
JPH11502018A (ja) * 1995-03-14 1999-02-16 チャンドラー，ハワード，ミルン サンプル収集装置
JP2001009812A (ja) * 1999-06-28 2001-01-16 Takashi Ueda セルロースと炭の混合材料及びその製造方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11502018A (ja) * 1995-03-14 1999-02-16 チャンドラー，ハワード，ミルン サンプル収集装置
JPH10237798A (ja) * 1996-12-12 1998-09-08 Elf Antar Fr 汚染液体用濾紙
JPH1114512A (ja) * 1997-06-20 1999-01-22 Tomakomai Rinshiyou Kensa Center:Kk 消臭採便容器、糞便臭抑制方法および消臭剤
JP2001009812A (ja) * 1999-06-28 2001-01-16 Takashi Ueda セルロースと炭の混合材料及びその製造方法

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006101137A1 (fr) * 2005-03-22 2006-09-28 Kobayashi Pharmaceutical Co., Ltd. Appareil pour déterminer le stade de ménopause
JP2006266764A (ja) * 2005-03-22 2006-10-05 Kobayashi Pharmaceut Co Ltd 更年期ステージ判定具
JP4610385B2 (ja) * 2005-03-22 2011-01-12 小林製薬株式会社 更年期ステージ判定具

Also Published As

Publication number Publication date
JPWO2002101386A1 (ja) 2005-05-26

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