WO2002101370A1 - Systeme microchimique - Google Patents

Systeme microchimique Download PDF

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Publication number
WO2002101370A1
WO2002101370A1 PCT/JP2002/005695 JP0205695W WO02101370A1 WO 2002101370 A1 WO2002101370 A1 WO 2002101370A1 JP 0205695 W JP0205695 W JP 0205695W WO 02101370 A1 WO02101370 A1 WO 02101370A1
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WO
WIPO (PCT)
Prior art keywords
light
lens
detector
plate
optical system
Prior art date
Application number
PCT/JP2002/005695
Other languages
English (en)
Japanese (ja)
Inventor
Jun Yamaguchi
Akihiko Hattori
Takehiko Kitamori
Original Assignee
Nippon Sheet Glass Co., Ltd.
Kanagawa Academy Of Science And Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nippon Sheet Glass Co., Ltd., Kanagawa Academy Of Science And Technology filed Critical Nippon Sheet Glass Co., Ltd.
Publication of WO2002101370A1 publication Critical patent/WO2002101370A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates

Definitions

  • the present invention condenses and irradiates a sample with excitation light to form a thermal lens, irradiates detection light and receives detection light transmitted through the sample, and detects the intensity of light caused by refraction by the thermal lens. It relates to a microchemical system that measures change. Background art
  • microchemical system using a glass substrate or the like. This aims to be able to perform all functions such as sample mixing, reaction, separation, extraction, and detection in a fine channel formed on a small glass substrate.
  • reactions performed in a microchemical system include diazotization reactions, nitration reactions, and antigen-antibody reactions.
  • extraction and separation include solvent extraction, electrophoretic separation, and column separation.
  • the electrophoresis apparatus is an apparatus for analyzing a very small amount of proteins, nucleic acids, and the like, and includes a plate-like member with a flow path composed of two glass substrates joined to each other. Since this member is plate-shaped, it is less susceptible to damage and easier to handle than a glass capillary tube having a circular or square cross section.
  • Micro-chemical systems have a very small amount of sample, so advanced detection methods Method is indispensable, but photothermal conversion spectroscopy using the thermal lens effect generated by the absorption of light by a sample in a fine channel opens the way to practical use.
  • advanced detection methods Method is indispensable, but photothermal conversion spectroscopy using the thermal lens effect generated by the absorption of light by a sample in a fine channel opens the way to practical use.
  • FIG. 6 is an explanatory diagram of the principle of the thermal lens.
  • the above-mentioned photothermal conversion effect is induced by irradiating the very small sample with the excitation light through the objective lens of the microscope.
  • the refractive index decreases with increasing temperature. Therefore, the sample irradiated with the excitation light condensed and irradiated has a large decrease in the refractive index as the temperature rises closer to the light-collecting center, and the thermal diffusion spreads farther away from the light-collecting center and closer to the periphery.
  • the degree of temperature rise is small, so that the decrease in the refractive index is small.
  • this refractive index distribution exhibits exactly the same effect as a concave lens, and this effect is called the thermal lens effect.
  • the magnitude of this effect ie the power of the concave lens, is proportional to the light absorption of the sample. Conversely, when the refractive index increases in proportion to the temperature, an effect similar to that of a convex lens is produced.
  • the photothermal conversion spectroscopy is suitable for detecting the diffusion of heat in a sample, that is, the refractive index of the sample, and thus detecting the concentration of an extremely small sample.
  • a plate member with a flow path is arranged below an objective lens of a microscope, and a predetermined wavelength output from an excitation light source is excited.
  • the generated light is incident on the microscope, and the objective lens of the microscope focuses and irradiates the sample in the analysis channel of the plate-shaped member with the channel.
  • a heat lens is formed around the condensing irradiation position of this condensing irradiation.
  • detection light having a wavelength different from that of the excitation light is emitted from the detection light source, and is emitted from the microscope after being incident on the microscope.
  • the emitted detection light is focused and irradiated on the thermal lens formed in the sample by the excitation light.
  • the detection light transmitted through the sample diverges or condenses due to the effect of the heat lens.
  • the light diverged or condensed from this sample and emitted is received by a detector as signal light via a condensing lens and a filter, or only a filter, and detected.
  • the intensity of the detected signal light depends on the refractive index of the thermal lens formed on the sample.
  • the heat lens is formed at the condensing irradiation position of the excitation light (hereinafter, referred to as the focal position), and the refractive index of the formed heat lens is described. Is detected by the detection light having a wavelength different from the wavelength of the excitation light.
  • the photothermal conversion spectrometer described above is large and lacks portability due to the complicated configuration of the light source, the optical system of the measurement unit and the detection unit (photoelectric conversion unit). For this reason, when performing analysis and the like using this photothermal conversion spectrometer, there is a problem that the installation place of the device and the operation of the device are limited, and the problem is that the work efficiency of the user is poor. There is.
  • the objective lens of the microscope is larger than the plate member with the flow path, which hinders miniaturization of the apparatus.
  • FIGS. 7A and 7B are explanatory diagrams of the formation position of the thermal lens and the focal position of the detection light in the traveling direction of the excitation light.
  • FIG. 7A shows a case where the objective lens has chromatic aberration.
  • B shows the case where the objective lens has no chromatic aberration.
  • the thermal lens 13 1 is formed at the focal position 13 2 of the excitation light and the focal position 13 3 of the detection light. Since 3 deviates from the focal position 13 2 of the excitation light by ⁇ L, the change in the refractive index of the thermal lens 13 1 can be detected as a change in the focal length of the detection light by this detection light.
  • the focus position 13 3 of the detection light is the focus position 13 2 of the excitation light, that is, the position of the thermal lens 13 1. It almost matches the position. As a result, the detection light does not undergo refraction by the heat lens 131, so that a change in the refractive index of the heat lens 131 cannot be detected.
  • An object of the present invention is to provide a microchemical system that can improve the work efficiency of a user and can be reduced in size. Disclosure of the invention
  • a plate-like member for accommodating an analyte, an excitation light source for outputting excitation light of a predetermined wavelength, and a wavelength different from the excitation light
  • a detection light source that outputs detection light
  • an incident optical system that causes the excitation light and the detection light to enter the analysis object coaxially
  • a detector that detects output light from the analysis object.
  • the microchemical system is provided in which the plate-shaped member is disposed separately from the incident optical system and the detector.
  • a rotation mechanism having a rotation axis, and rotating the incident optical system and the detector about the rotation axis while maintaining the relative positions of the incident optical system and the detector. It is preferable to prepare.
  • the incident optical system includes: a central axis optical path coinciding with a central axis in a direction in which the rotation axis extends; and a light reflected from the central axis optical path at a predetermined angle. It is preferable to include a first reflection means that rotates together with the first reflection means, and a second reflection means that reflects light reflected by the first reflection means toward the detector. .
  • a translation mechanism for moving the incident optical system and the detector in parallel to the plate surface of the plate-like member while maintaining the relative positions of the incident optical system and the detector. Is preferred.
  • the incident optical system includes: a reflecting unit that reflects light toward the detector; and a lens that is disposed on an optical path of light reflected by the reflecting unit.
  • the parallel moving mechanism includes a guide member extending parallel to the plate surface, and at least the reflecting means, the lens, and the detector. And a movable member guided by the guide member.
  • the incident optical system includes at least a reflecting unit that reflects light toward the detector, and a lens disposed on an optical path of the reflected light reflected by the reflecting unit. It is preferable to include a plurality of sets of the input units, and optical path changing means for changing the optical path of the reflected light toward each of the reflecting means.
  • the optical path changing unit changes an optical path of the reflected light by changing a reflection angle.
  • the optical path changing unit changes the optical path of the reflected light by moving in parallel with the optical path of the incident light.
  • the incident optical system includes a gradient index lens.
  • the lens is a gradient index lens.
  • the plate-shaped member has at least one flow path for storing the analyte.
  • FIG. 1 is a diagram showing a schematic configuration of a microchemical system according to a first embodiment of the present invention.
  • FIG. 2 is an explanatory diagram of a change in signal intensity with respect to a shift ⁇ L of the optimum focus position of the load lens 10 in FIG.
  • FIG. 3 is a diagram showing a schematic configuration of a microchemical system according to a second embodiment of the present invention.
  • FIG. 4 is a diagram showing a schematic configuration of a microchemical system according to a third embodiment of the present invention.
  • FIG. 5 is a diagram showing a schematic configuration of a microchemical system according to a fourth embodiment of the present invention.
  • Figure 6 is an explanatory diagram of the principle of the heat lens.
  • FIG. 7 is an explanatory diagram of a heat lens formation position and a focus position of detection light with respect to a traveling direction of excitation light.
  • FIG. 7A shows a case where the objective lens has chromatic aberration
  • FIG. 2 shows a case where the objective lens has no chromatic aberration.
  • FIG. 8 is a diagram illustrating a method of detecting a change in the refractive index of a thermal lens in a conventional microchemical system.
  • FIG. 8A illustrates a thermal lens formed in front of a focal position of an objective lens.
  • FIG. 8B shows a case in which a thermal lens is formed behind the focal position of the objective lens.
  • FIG. 9 is an explanatory diagram of a method of detecting a change in the refractive index of a thermal lens in a conventional microchemical system, and shows a case where the detection light is spread using a di-purging lens.
  • FIG. 1 is a diagram showing a schematic configuration of a microchemical system according to a first embodiment of the present invention.
  • the microchemical system 1 includes an excitation light source 41 that outputs excitation light of a predetermined wavelength, a detection light source 42 that outputs detection light having a wavelength different from that of the excitation light, and a plate-like shape that accommodates a sample (analyte).
  • the member 30 includes an incident optical system that causes the excitation light and the detection light to enter the sample coaxially, and a detector 51 that detects output light from the sample. Of these, the incident optical system and the detector 51 are described later.
  • the rotating mechanism is composed of a jig 200 and a rotating shaft 21.
  • a chimney 43 for modulating the excitation light is arranged on the optical path of the excitation light output from the excitation light source 41.
  • a dichroic mirror 44 is arranged downstream of the chile 43 in the direction of travel of the excitation light.
  • the detection light and the excitation light enter the dichroic mirror 45, the optical paths of the two coincide.
  • the light whose optical path coincides is referred to as coaxial light.
  • a mirror 23 is arranged on the optical path of the coaxial light.
  • the optical path of the coaxial light whose traveling direction has been changed by the mirror 23 coincides with the central axis in the direction in which the rotating shaft 21 described later extends.
  • the rotating shaft 21 is rotated by a motor 22 connected to one end (the lower end in the drawing).
  • a mirror 24 (first reflecting means) is provided on the other end side (the upper end side in the drawing).
  • a jig 20 is provided on the rotating shaft 21 in a cantilever manner.
  • the jig 20 extends in a direction orthogonal to the direction in which the rotation axis 21 extends, and a rod lens 10 of a refractive index distribution type penetrates and is fixed near the tip.
  • the optical axis of the rod lens 10 is parallel to the direction in which the central axis of the rotation axis 21 extends.
  • a mirror 25 (second reflecting means) is arranged on the upper surface closer to the tip than the rod lens 10. The mirror 25 is located above the aperture lens 10, and the coaxial light whose traveling direction has been changed by the mirror 24 changes its traveling direction again by the mirror 25. It is changed and enters the mouth lens 10.
  • the O lens 10 is made of a cylindrical transparent body whose refractive index continuously changes from the center to the periphery (for example, Japanese Patent Publication No. 63-63502).
  • glass or plastic is used. It is manufactured by Both end surfaces of the rod lens 10 are planes perpendicular to the optical axis of the rod lens 10.
  • the rod lens 10 is located at a distance r from the center axis in the radial direction.
  • N (r) is the on-axis refractive index.
  • the square distribution constant as g approximately a quadratic equation with respect to r
  • n (r) n 0 I 1 - (g 2/2) ⁇ r 2
  • Rod lens 10 has its length z. Where 0 ⁇ z. When selected within the range of rZ 2 g, the imaging characteristics are the same as those of a normal convex lens, although both surfaces are flat.
  • a focus is created at the location.
  • the rod lens 10 is manufactured, for example, by the following method.
  • the rod lens 10 is set so that the focus position of the detection light slightly deviates by ⁇ ⁇ ⁇ ⁇ ⁇ from the focus position of the excitation light (Fig. 7 ⁇ )
  • the above AL value changes depending on the thickness of the sample (analyte) to be measured.
  • ⁇ L 3 ⁇ Ic.
  • Such a rod lens 10 has a cylindrical shape, it can be easily attached to the jig 20.
  • the microchemical system 1 can be downsized.
  • the gradient index lens has an appropriate chromatic aberration, the focus positions of both the excitation light and the detection light can be shifted only by the rod lens 10. Therefore, it is not necessary to arrange a plurality of lenses, and the rod lens 10 can contribute to the miniaturization of the microchemical system 1 from this point as well.
  • a plate-like member 30 is arranged in parallel with the jig 20.
  • the plate-shaped member 30 is composed of glass substrates 31, 32, and 33 stacked in three layers.
  • a flow path 34 for mixing, stirring, synthesizing, separating, extracting, detecting, etc., the sample is formed in the intermediate glass substrate 32.
  • the material of the plate member 30 is desirably glass from the viewpoint of durability and chemical resistance, but may be an organic substance such as plastic if the use is limited.
  • the sample is, for example, a biological sample such as a cell for the purpose of DNA analysis
  • glass having high acid resistance and high resistance to acid is preferable among the glasses.
  • Specific examples include borosilicate glass, soda lime glass, alumino borosilicate glass, and quartz glass.
  • a wavelength filter 52 is disposed to separate light into light and selectively transmit only the detection light. Below this wavelength filter 52, a photoelectric converter 51 as a detector is arranged.
  • the photoelectric converter 51 and the wavelength filter 52 are disposed at the tip of a jig 26 provided on the rotating shaft 21 in a cantilever manner.
  • the jig 26 also extends in a direction orthogonal to the direction in which the rotation axis 21 extends, similarly to the jig 20, and the jig 26 is disposed at a position parallel to the jig 20.
  • the mirrors 24 and 25 of the incident optical system, the lens 10 and the detector 51 remain the same relative to each other while maintaining their positions relative to the rotation axis. Can be freely rotated around the rotary shaft 21 of the motor.
  • transmitted light the detection light transmitted through the sample in the flow path 34 of the plate member 30 (hereinafter, referred to as transmitted light).
  • the signal obtained by the photoelectric converter 51 receiving this transmitted light was sent to the lock-in amplifier 53 in order to improve the S / N ratio in synchronization with the chiyotsuba 43. It is later analyzed by the computer 54. If the signal strength is weak, a preamplifier may be inserted between the photoelectric converter 51 and the lock-in amplifier 53. Further, a pinhole for transmitting only a part of the transmitted light may be arranged upstream of the photoelectric converter 51 in the traveling direction of the transmitted light.
  • the photoelectric converter 51 and the wavelength filter 52 are not rotated together with the rod lens 10 or the like, but are turned for each measurement point.
  • a pair of photoelectric converters 51 and a wavelength filter 52 may be provided.
  • prisms may be used instead of mirrors 23, 24, and 25, respectively.
  • the lens 10 can be moved, there is no need to move the sample when changing the measurement point. For this reason, the flow of the sample in the flow channel is not disturbed as in the case of moving the sample. Therefore, it is not necessary to wait until the turbulence of the flow has subsided, and quick measurement can be performed, thereby improving the work efficiency of the user.
  • a rod lens 10 for irradiating the sample with coaxial light composed of the excitation light and the detection light and a mirror 25 for guiding the coaxial light to the rod lens 10 are provided in a jig 20.
  • the optical system can be simplified by separating the rotating mechanism from the light sources 41 and 42 because the rotating mechanism is fixed and constitutes a single rotating mechanism. The entire system can be downsized.
  • the light sources 41 and 42 may be fixed to the jig 20.In this case, since the optical path of the coaxial light and the optical axis of the rod lens 10 are fixed, environmental changes such as temperature changes On the other hand, there is an advantage that the optical path of the coaxial light and the optical axis of the rod lens 10 are not easily shifted.
  • FIG. 3 is a diagram showing a schematic configuration of a microchemical system according to the second embodiment of the present invention.
  • FIG. 3 the same components as those of the microchemical system 1 shown in FIG. 1 among the components of the microchemical system 2 are denoted by the same reference numerals, and description thereof is omitted.
  • the microchemical system 2 is provided with a parallel movement mechanism for moving the incident optical system and the detector 51 in parallel to the plate surface of the plate member 30 while maintaining the relative positions.
  • This parallel moving mechanism is a stage 61 (guide member) arranged parallel to the plate member 30 above the plate member 30 and is guided by the stage 61 to move parallel to the plate member 30.
  • Carrier 6 2 movable member
  • the carrier 62 has an upper end located above the plate member 30 and a lower end located below the plate member 30.
  • Rod lens 10 on top A unit 60 equipped with and a mirror 25 is mounted.
  • the mirror 25 is fixed in a posture in which the coaxial light passes through the load lens 10 and enters the photoelectric converter 51.
  • a photoelectric converter 51 and a wavelength filter 52 are mounted.
  • a mirror 24 for guiding coaxial light to the rod lens 10 via the mirror 25 is disposed above one end of the stage 61.
  • the mirror 24 is fixed at an angle at which the reflected coaxial light travels parallel to the stage 61 and reaches the mirror 25.
  • the optical path of the coaxial light reflected by the mirror 24 and the unit 6 Since the moving direction of 0 is parallel and invariable, the optical arrangement of the coaxial light and the mirror 25 and the rod lens 10 is always constant. Therefore, it is not necessary to adjust the mirror 25, and the coaxial light is always guided to the rod lens 10 and the photoelectric converter 51. Also, since no mechanism for adjusting the optical axis is required, the size of the microchemical system 2 can be reduced.
  • the distance between the load lens 10 and the flow path 34 of the plate member 30 is constant even if the unit 60 moves. is there. Therefore, it is not necessary to focus on each measurement point, and the work efficiency of the user is good.
  • the photoelectric converter 51 and the wavelength filter 52 are not moved in parallel with the rod lens 10 or the like, but are measured.
  • a set of a photoelectric converter 51 and a wavelength filter 52 may be provided for each point. Further, an array-like photoelectric converter may be provided.
  • FIG. 4 is a diagram showing a schematic configuration of a microchemical system according to a third embodiment of the present invention.
  • a mirror 24 optical path changing means for changing the optical path of the coaxial light toward the incident unit 70 described later is arranged above the plate member 30. ing. The mirror 24 can be rotated to change the angle of the reflecting surface.
  • a plurality of entrance units 70 each having a set of a rod lens 10 and a mirror 25 are arranged.
  • the injection unit 70 is arranged above each measurement point for each measurement point of the plate member 30, and the optical axis of the rod lens 10 passes through the measurement point.
  • Each mirror 25 is arranged in such a position that the optical path of the coaxial light traveling from the mirror 24 coincides with the optical axis of the load lens 10. Therefore, the angle of the mirror 25 differs for each incident unit 70.
  • the coaxial light that has passed through the rod lens 10 passes through the measurement point.
  • a wavelength filter 52 and a photoelectric converter 51 are arranged below the plate member 30 in order to detect output light passing through the measurement point.
  • the photoelectric converter 51 is arranged for each measurement point.
  • the photoelectric converter 51 may be an array-like photoelectric converter.
  • the load lenses 10 are arranged at different measurement points, measurement at different measurement points can be performed quickly, thereby improving the user's work efficiency. Can be realized. Since the rod lens 10 is extremely small, even if a plurality of rod lenses are provided, it does not hinder the miniaturization of the microchemical system 3.
  • the mirror 24 may be moved along the optical path of the coaxial light incident on the mirror 24.
  • a prism may be used instead of the mirror 24.
  • a light source for excitation light and detection light may be provided for each rod lens 10 o
  • FIG. 5 is a schematic diagram of a microchemical system according to the fourth embodiment of the present invention. It is a figure which shows schematic structure.
  • FIG. 5 of the components of the microchemical system 5, the same components as those of the microchemical system described above are denoted by the same reference numerals, and description thereof is omitted.
  • a plate member 30 is provided between the incident optical system and the photoelectric converter 51, similarly to the above various microchemical systems.
  • the plate member 30 is arranged on a jig 27.
  • the photoelectric converter 51 disposed below the plate-shaped member 30 is connected to a computer 54 via a preamplifier 50 and a mouthpiece 53.
  • a refractive index distribution type Brainer lens 80 attached to the tip of the jig 20 is located above the plate-shaped member 30, a refractive index distribution type Brainer lens 80 attached to the tip of the jig 20 is located.
  • the planar lens 80 has a spherical shape (the shape of a portion obtained by cutting a sphere along a surface orthogonal to the radial direction), and the flat surface 80 a is formed of a glass substrate 80. 1 is on the same plane as 8a.
  • the planar lens 80 has a higher refractive index toward the center of the lens. This gradient of the refractive index can be formed by an ion exchange method in which sodium ions in the glass substrate 81 are replaced with thallium ions and potassium ions.
  • the ion exchange is performed by coating and masking the metal film except for the part of the upper surface 81a where the brain lens 80 is to be formed, and then converting it to the solvent salt of the nitric acid and nitric acid rim. This is done by immersion.
  • the distribution of the refractive index of the planar lens 80 is similar to the refractive index distribution of the rod lens 10 described above.
  • the lens can be integrally formed on a glass substrate, mounting and holding are easy.
  • the flat end surface of the lens is in the same plane as the surface of the glass substrate, and this plane is orthogonal to the optical axis of the lens. Therefore, it is easy to align the optical axis of the lens with the optical path of the coaxial light. It is manufactured separately from the glass substrate 81.
  • the planar lens 80 thus obtained may be incorporated in the glass substrate 81.
  • the plate-shaped member is disposed separately from the incident optical system and the detector, the measurement position can be easily changed. Thus, the work efficiency of the user can be improved.
  • the incident optical system and the detector can rotate around the rotation axis while maintaining the relative positions of the incident optical system and the detector, it is not necessary to move the plate member when changing the measurement position. Measurement can be performed quickly and, consequently, the work efficiency of the user can be further improved and the size can be reduced as a microchemical system that can measure at different measurement points. .
  • the parallel moving mechanism moves the incident optical system and the detector in parallel with the plate surface of the plate member while maintaining the relative positions of the incident optical system and the detector. Measurement can be performed quickly without the need to move the light source, thereby improving the user's work efficiency and eliminating the need for a mechanism to adjust the relative positions of the incident optical system and the detector.
  • the microchemical system can be reliably downsized.
  • the reflecting means of each of the plurality of sets of the input optics of the input optical system reflects the light toward at least the detector, and transmits the reflected light to a lens on the optical path of the reflected light. Since the optical path changing means changes the optical path toward each of the reflecting means, measurement at different measurement points can be performed quickly, thereby improving the work efficiency of the user and measuring at different measurement points. It can be downsized as a microchemical system that can be used.
  • the space occupied by the lens is considerably smaller than that of a conventional system provided with a lens. Can be further miniaturized.
  • the lens is a gradient index lens, the space occupied by the lens is considerably smaller than that of a lens having a normal lens, and therefore, the microchemical system is further increased. It can be downsized.
  • the plate-shaped member has at least one flow path for accommodating the analyte, different measurement points can be easily set on the flow path, thereby improving the work efficiency of the user. It can be further improved.

Abstract

L'invention concerne un système microchimique (1) permettant d'améliorer l'efficacité de travail de l'utilisateur, ce système comprenant un système optique d'entrée disposé dans un mécanisme de rotation composé de montages (20, 26), d'un arbre rotatif (21) et d'un capteur (51). Une lentille barreau (10) est fixée au montage (20) et s'étend à travers ledit montage qui est en porte-à-faux par rapport à l'arbre rotatif (21). Un élément de type plaque (30) est disposé en-dessous de la lentille barreau (10). Un transducteur photoélectrique (51) servant à détecter la lumière de sortie transmise à travers des échantillons contenus dans l'élément de type plaque (30) est disposé en-dessous dudit élément de type plaque (30) à l'extrémité du montage (26) en porte-à-faux par rapport à l'arbre rotatif (21). On peut modifier la position du transducteur photoélectrique (51) en faisant pivoter les montages (20, 26) de façon à permettre la détection de la lumière de sortie transmise à travers les échantillons contenus dans l'élément de type plaque (30).
PCT/JP2002/005695 2001-06-07 2002-06-07 Systeme microchimique WO2002101370A1 (fr)

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009210323A (ja) * 2008-03-03 2009-09-17 Nippon Sheet Glass Co Ltd 多チャンネル熱レンズ分光分析システム及び多チャンネル熱レンズ分光分析方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10232210A (ja) * 1997-02-19 1998-09-02 Bunshi Bio Photonics Kenkyusho:Kk 光熱変換分光分析装置
JP2000002677A (ja) * 1998-06-15 2000-01-07 Asahi Chem Ind Co Ltd 分析装置
JP2001059829A (ja) * 1999-08-25 2001-03-06 Univ Osaka Sangyo 光熱レンズ型試料分析装置

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57179055A (en) * 1981-04-24 1982-11-04 Nippon Sheet Glass Co Ltd Manufacture of refractive index distribution type lens
JPS63115548A (ja) * 1986-10-31 1988-05-20 株式会社島津製作所 光線ｃｔ装置
JPH07119590B2 (ja) * 1989-08-09 1995-12-20 サンスター技研株式会社 薄膜状塗布剤の検査装置
JPH05312544A (ja) * 1992-05-14 1993-11-22 Asahi Glass Co Ltd ガラス板上のプリント線の検査方法及び装置
JPH1194845A (ja) * 1997-09-16 1999-04-09 Tokimec Inc 試料注入状態検査装置
US6100535A (en) * 1998-01-29 2000-08-08 The Regents Of The University Of California Rotary confocal scanner for detection of capillary arrays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10232210A (ja) * 1997-02-19 1998-09-02 Bunshi Bio Photonics Kenkyusho:Kk 光熱変換分光分析装置
JP2000002677A (ja) * 1998-06-15 2000-01-07 Asahi Chem Ind Co Ltd 分析装置
JP2001059829A (ja) * 1999-08-25 2001-03-06 Univ Osaka Sangyo 光熱レンズ型試料分析装置

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