WO2002079507A3 - Verfahren zur spezifischen detektion, isolation und charakterisierung von zellen aus körperproben durch transfektion von nukleinsäurekonstrukten - Google Patents

Verfahren zur spezifischen detektion, isolation und charakterisierung von zellen aus körperproben durch transfektion von nukleinsäurekonstrukten Download PDF

Info

Publication number
WO2002079507A3
WO2002079507A3 PCT/EP2002/003480 EP0203480W WO02079507A3 WO 2002079507 A3 WO2002079507 A3 WO 2002079507A3 EP 0203480 W EP0203480 W EP 0203480W WO 02079507 A3 WO02079507 A3 WO 02079507A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
nucleic acid
acid constructs
isolating
body samples
Prior art date
Application number
PCT/EP2002/003480
Other languages
English (en)
French (fr)
Other versions
WO2002079507A2 (de
Inventor
Ralph Wirtz
Original Assignee
Bayer Ag
Ralph Wirtz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10133930A external-priority patent/DE10133930A1/de
Priority claimed from DE10160271A external-priority patent/DE10160271A1/de
Application filed by Bayer Ag, Ralph Wirtz filed Critical Bayer Ag
Priority to US10/474,078 priority Critical patent/US20040219543A1/en
Priority to EP02712960A priority patent/EP1385994A2/de
Priority to AU2002244757A priority patent/AU2002244757A1/en
Priority to JP2002577916A priority patent/JP2005525779A/ja
Publication of WO2002079507A2 publication Critical patent/WO2002079507A2/de
Publication of WO2002079507A3 publication Critical patent/WO2002079507A3/de
Priority to US12/578,618 priority patent/US20100099108A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Detektion krankheits-assoziierter Zellen aus Körperproben das dadurch gekennzeichnet ist, dass die Zellen der Körperprobe mit Nukleinsäurekonstrukten transfiziert oder infiziert werden die folgende Komponenten beinhalten: a) ein Promoter-Element mit mindestens einer DNA-Bindungsstelle für Transkriptionsfaktoren, die auf Grund der krankhaften Veränderung der Zellen eine veränderte Aktivität aufweisen und b) ein Reportergen, das die Detektion der krankhaften Zellen ermöglicht und anschliessend die transfizierten oder infizierten Zellen detektiert.
PCT/EP2002/003480 2001-04-02 2002-03-28 Verfahren zur spezifischen detektion, isolation und charakterisierung von zellen aus körperproben durch transfektion von nukleinsäurekonstrukten WO2002079507A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/474,078 US20040219543A1 (en) 2001-04-02 2002-03-28 Method for specifically detecting, isolating and characterizing cells from body samples by transfecting nucleic acid constructs
EP02712960A EP1385994A2 (de) 2001-04-02 2002-03-28 Verfahren zur spezifischen detektion, isolation und charakterisierung von zellen aus körperproben durch transfektion von nukleinsäurekonstrukten
AU2002244757A AU2002244757A1 (en) 2001-04-02 2002-03-28 Method for specifically detecting, isolating and characterizing cells from body samples by transfecting nucleic acid constructs
JP2002577916A JP2005525779A (ja) 2001-04-02 2002-03-28 核酸構築物のトランスフェクションによって、身体サンプルから細胞を特異的に検出、単離および特徴付けするための方法
US12/578,618 US20100099108A1 (en) 2001-04-02 2009-10-14 Method for Detecting, Isolating, and Characterizing Cells from Body Samples by Transfection with Nucleic Acid Constructs

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE10116323 2001-04-02
DE10116323.1 2001-04-02
DE10133930A DE10133930A1 (de) 2001-04-02 2001-07-12 Verfahren zur spezifischen Detektion, Isolation und Charakterisierung von Zellen aus Körperproben durch Transfektion von Nukleinsäurekonstrukten
DE10133930.5 2001-07-12
DE10160271.5 2001-12-07
DE10160271A DE10160271A1 (de) 2001-12-07 2001-12-07 Verfahren zur spezifischen Detektion, Isolation und Charakterisierung von Zellen aus Körperproben durch Transfektion von Nukleinsäurekonstrukten

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/578,618 Continuation US20100099108A1 (en) 2001-04-02 2009-10-14 Method for Detecting, Isolating, and Characterizing Cells from Body Samples by Transfection with Nucleic Acid Constructs

Publications (2)

Publication Number Publication Date
WO2002079507A2 WO2002079507A2 (de) 2002-10-10
WO2002079507A3 true WO2002079507A3 (de) 2003-11-06

Family

ID=27214381

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/003480 WO2002079507A2 (de) 2001-04-02 2002-03-28 Verfahren zur spezifischen detektion, isolation und charakterisierung von zellen aus körperproben durch transfektion von nukleinsäurekonstrukten

Country Status (5)

Country Link
US (2) US20040219543A1 (de)
EP (1) EP1385994A2 (de)
JP (1) JP2005525779A (de)
AU (1) AU2002244757A1 (de)
WO (1) WO2002079507A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070161031A1 (en) * 2005-12-16 2007-07-12 The Board Of Trustees Of The Leland Stanford Junior University Functional arrays for high throughput characterization of gene expression regulatory elements
US20090018031A1 (en) * 2006-12-07 2009-01-15 Switchgear Genomics Transcriptional regulatory elements of biological pathways tools, and methods
EP2135080A4 (de) * 2007-03-08 2010-12-01 Switchgear Genomics Funktionelle arrays zur charakterisierung mit hohem durchsatz von regulatorischen elementen in nichttranslatierten bereichen von genen
US8815779B2 (en) * 2009-09-16 2014-08-26 SwitchGear Genomics, Inc. Transcription biomarkers of biological responses and methods
CA2865642C (en) 2012-03-14 2021-10-26 Salk Institute For Biological Studies Adenoviral tumor diagnostics
EP2971008B1 (de) 2013-03-14 2018-07-25 Salk Institute for Biological Studies Onkolytische adenoviruszusammensetzungen
WO2017147265A1 (en) 2016-02-23 2017-08-31 Salk Institute For Biological Studies High throughput assay for measuring adenovirus replication kinetics
KR102471633B1 (ko) 2016-02-23 2022-11-25 솔크 인스티튜트 포 바이올로지칼 스터디즈 바이러스 동역학에 미치는 영향 최소화를 위한 치료용 아데노바이러스의 외인성 유전자 발현
CN110062630A (zh) 2016-12-12 2019-07-26 萨克生物研究学院 肿瘤靶向合成腺病毒及其用途
CN112168820B (zh) * 2019-07-05 2023-09-29 中国科学院生物物理研究所 SRCAP ATPase抑制剂在结直肠癌治疗中的应用
WO2021079522A1 (ja) * 2019-10-25 2021-04-29 株式会社 東芝 腫瘍細胞群の特性を決定する方法、キット及びプログラム

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998037235A1 (en) * 1997-02-24 1998-08-27 Cornell Research Foundation, Inc. Method of screening agents as candidates for drugs or sources of drugs
US5851775A (en) * 1997-03-20 1998-12-22 Johns Hopkins University β-catenin, Tcf-4, and APC interact to prevent cancer
US5914233A (en) * 1996-08-23 1999-06-22 Osteo Screen Screening assay for the identification of agents which alter expression of PTH-rP
US6140052A (en) * 1997-03-20 2000-10-31 The Johns Hopkins University cMYC is regulated by Tcf-4
WO2000070089A1 (en) * 1999-05-14 2000-11-23 The Johns Hopkins University Cdx2 is downstream mediator of apc tumor suppressor activity
WO2002044378A2 (en) * 2000-11-28 2002-06-06 Curis, Inc. Wnt signalling assay, methods and uses thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GR860984B (en) * 1985-04-17 1986-08-18 Zymogenetics Inc Expression of factor vii and ix activities in mammalian cells
US5968738A (en) * 1995-12-06 1999-10-19 The Board Of Trustees Of The Leland Stanford Junior University Two-reporter FACS analysis of mammalian cells using green fluorescent proteins
DE19701141C1 (de) * 1997-01-16 1998-04-09 Hoechst Ag Genkonstrukte für durch Proteasen aktivierbare Wirksubstanzen
US6167052A (en) * 1998-04-27 2000-12-26 Vpnx.Com, Inc. Establishing connectivity in networks
US7155518B2 (en) * 2001-01-08 2006-12-26 Interactive People Unplugged Ab Extranet workgroup formation across multiple mobile virtual private networks
US7500069B2 (en) * 2001-09-17 2009-03-03 Hewlett-Packard Development Company, L.P. System and method for providing secure access to network logical storage partitions
US7062566B2 (en) * 2002-10-24 2006-06-13 3Com Corporation System and method for using virtual local area network tags with a virtual private network

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5914233A (en) * 1996-08-23 1999-06-22 Osteo Screen Screening assay for the identification of agents which alter expression of PTH-rP
WO1998037235A1 (en) * 1997-02-24 1998-08-27 Cornell Research Foundation, Inc. Method of screening agents as candidates for drugs or sources of drugs
US5851775A (en) * 1997-03-20 1998-12-22 Johns Hopkins University β-catenin, Tcf-4, and APC interact to prevent cancer
US6140052A (en) * 1997-03-20 2000-10-31 The Johns Hopkins University cMYC is regulated by Tcf-4
WO2000070089A1 (en) * 1999-05-14 2000-11-23 The Johns Hopkins University Cdx2 is downstream mediator of apc tumor suppressor activity
WO2002044378A2 (en) * 2000-11-28 2002-06-06 Curis, Inc. Wnt signalling assay, methods and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KORINEK V ET AL: "CONSTITUTIVE TRANSCRIPTIONAL ACTIVATION BA A BETA-CATETNIN-TCF COMPLEX IN APC-/- COLON CARCINOMA", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 275, 21 March 1997 (1997-03-21), pages 1784 - 1787, XP002070159, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
JP2005525779A (ja) 2005-09-02
US20040219543A1 (en) 2004-11-04
AU2002244757A1 (en) 2002-10-15
WO2002079507A2 (de) 2002-10-10
EP1385994A2 (de) 2004-02-04
US20100099108A1 (en) 2010-04-22

Similar Documents

Publication Publication Date Title
WO2004081174A3 (ja) 間葉系幹細胞検出用マーカー及び該マーカーを用いた間葉系幹細胞の識別方法
SG134283A1 (en) Biomarkers for liver fibrotic injury
Wang et al. Disorders in angiogenesis and redox pathways are main factors contributing to the progression of rheumatoid arthritis: a comparative proteomics study
WO2003062462A3 (en) Method for isolating nucleic acids and protein from a single sample
WO2005095964A3 (en) Methods and compositions for the detection of cervical disease
WO2003042397A3 (en) A method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby
WO2004046098A3 (en) Method for predicting autoimmune diseases
WO2001092565A8 (en) Diagnosis of diseases associated dna transcription by means of assessing the methylation status of genes associated with dna transcription
WO2006031800A3 (en) Reverse transfection of cell arrays
WO2006137932A3 (en) Homogeneous analyte detection
WO1999036516A3 (en) Method of determining the function of nucleotide sequences and the proteins they encode by transfecting the same into a host
EP1323728A3 (de) Verfahren zur Erstellung eines Standard Gen Transkriptions Musters für die Diagnostik
WO2002079507A3 (de) Verfahren zur spezifischen detektion, isolation und charakterisierung von zellen aus körperproben durch transfektion von nukleinsäurekonstrukten
WO2004092708A3 (en) Methods for the electrochemical detection of target compounds
WO2004063706A3 (en) Method of detecting over-expression of t-cell receptor genes by real-time pcr
WO2003027309A3 (en) Diagnostic probe detection system
WO2005003387A3 (en) Specific method of prostate cancer detection based on pca3 gene, and kits therefor
WO2005083115A3 (de) Verfahren zur erkennung von sepsis
WO2003052099A3 (en) Methods of parallel gene cloning and analysis
WO2005113802A3 (en) Genotoxic testing
WO2003014388A3 (en) Method and nucleic acids for the analysis of colon cancer
WO2005017186A3 (en) Method for rapid identification of alternative splicing
WO2001068913A3 (en) Nucleic acid detection method and system
WO2003072828A3 (en) Fcgammariib polymorphisms detection in system lupus erythematosus
WO2004108957A3 (fr) Procede de diagnostic et/ou de pronostic d'un syndrome septique

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2002712960

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2002577916

Country of ref document: JP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 2002712960

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 10474078

Country of ref document: US