WO2002068452A3 - Procede d'isolement de composes charges - Google Patents

Procede d'isolement de composes charges Download PDF

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Publication number
WO2002068452A3
WO2002068452A3 PCT/GB2002/000770 GB0200770W WO02068452A3 WO 2002068452 A3 WO2002068452 A3 WO 2002068452A3 GB 0200770 W GB0200770 W GB 0200770W WO 02068452 A3 WO02068452 A3 WO 02068452A3
Authority
WO
WIPO (PCT)
Prior art keywords
membrane
compound
charged
microns
effective
Prior art date
Application number
PCT/GB2002/000770
Other languages
English (en)
Other versions
WO2002068452A2 (fr
WO2002068452B1 (fr
Inventor
Michael Naldrett
Original Assignee
Plant Bioscience Ltd
Michael Naldrett
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plant Bioscience Ltd, Michael Naldrett filed Critical Plant Bioscience Ltd
Priority to AU2002232016A priority Critical patent/AU2002232016A1/en
Priority to US10/468,799 priority patent/US20040069707A1/en
Priority to JP2002567962A priority patent/JP2004537033A/ja
Priority to EP02712109A priority patent/EP1368370A2/fr
Publication of WO2002068452A2 publication Critical patent/WO2002068452A2/fr
Publication of WO2002068452A3 publication Critical patent/WO2002068452A3/fr
Publication of WO2002068452B1 publication Critical patent/WO2002068452B1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
    • B01J20/28033—Membrane, sheet, cloth, pad, lamellar or mat
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00—Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20—Anion exchangers for chromatographic processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/12—Ion-exchange processes in general; Apparatus therefor characterised by the use of ion-exchange material in the form of ribbons, filaments, fibres or sheets, e.g. membranes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention porte sur un procédé d'isolement d'un composé chargé, spécialement de type protéique consistant: à placer un échantillon préparé du composé dans une solution; à mettre en contact l'échantillon préparé avec une membrane dont la surface absorbante efficace est limitée de manière à permettre la fixation réversible du composé chargé à la membrane pour que le solvant puisse en être extrait et que le composé chargé y reste quasi réversiblement fixé; puis à éluter le composé de la membrane. Les composés élutés peuvent alors être directement soumis à une analyse par spectroscopie de masse en vue, par exemple, d'en caractériser les différents peptides.
PCT/GB2002/000770 2001-02-22 2002-02-21 Procede d'isolement de composes charges WO2002068452A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2002232016A AU2002232016A1 (en) 2001-02-22 2002-02-21 Method of isolating charged compounds, especially peptides and proteins
US10/468,799 US20040069707A1 (en) 2001-02-22 2002-02-21 Method of isolating a charged compound
JP2002567962A JP2004537033A (ja) 2001-02-22 2002-02-21 荷電化合物の単離方法
EP02712109A EP1368370A2 (fr) 2001-02-22 2002-02-21 Procede d'isolement de composes charges, particulierement peptides et proteines

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0104429.6 2001-02-22
GB0104429A GB2372464B (en) 2001-02-22 2001-02-22 Method of isolating a charged compound

Publications (3)

Publication Number Publication Date
WO2002068452A2 WO2002068452A2 (fr) 2002-09-06
WO2002068452A3 true WO2002068452A3 (fr) 2003-10-16
WO2002068452B1 WO2002068452B1 (fr) 2004-02-19

Family

ID=9909313

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/000770 WO2002068452A2 (fr) 2001-02-22 2002-02-21 Procede d'isolement de composes charges

Country Status (6)

Country Link
US (1) US20040069707A1 (fr)
EP (1) EP1368370A2 (fr)
JP (1) JP2004537033A (fr)
AU (1) AU2002232016A1 (fr)
GB (1) GB2372464B (fr)
WO (1) WO2002068452A2 (fr)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA06000347A (es) 2003-07-08 2006-03-28 Genentech Inc Polipeptidos heterologos il-17 a/f y usos terapeuticos de los mismos.
US7906630B2 (en) * 2004-04-27 2011-03-15 PerkinElmer Heath Sciences, Inc. Method for identifying peptides in a biological sample
WO2009032293A1 (fr) * 2007-09-06 2009-03-12 Waters Investments Limited Procédés de séparation de macromolécules fonctionnalisées
DE102008018734B4 (de) 2008-04-14 2013-03-21 Sartorius Stedim Biotech Gmbh Hydrophobe Cellulose-Membran, Verfahren zu ihrer Herstellung und ihre Verwendung in der hydrophoben Interaktionschromatographie
DE102008055821A1 (de) * 2008-04-14 2009-10-15 Sartorius Stedim Biotech Gmbh Cellulosehydrat-Membran, Verfahren zur ihrer Herstellung und Verwendung davon
DE102008018732B4 (de) * 2008-04-14 2022-06-09 Sartorius Stedim Biotech Gmbh Verfahren zur Stofftrennung unter Verwendung einer Cellulosehydrat-Membran in der Größenausschlusschromatographie
US20090317400A1 (en) 2008-05-05 2009-12-24 Krzysztof Masternak Anti-IL 17A/IL-17F Cross-Reactive Antibodies and Methods of Use Thereof
RS59232B1 (sr) 2008-08-14 2019-10-31 Genentech Inc Postupci za uklanjanje zagađivača koristeći hromatografiju membrane razmene jona sa razmeštanjem autohtonih proteina

Citations (2)

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WO1999060394A1 (fr) * 1998-05-15 1999-11-25 Merck Patent Gmbh Colonne de rectification miniaturisee
WO2000055185A1 (fr) * 1999-03-15 2000-09-21 Millipore Corporation Membranes chargees en metal et liees a un ligand pour chromatographie par affinite avec un ion metallique

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US5124041A (en) * 1989-07-28 1992-06-23 Applied Biosystems, Inc. Biomolecule sample immobilization
US5078978A (en) * 1989-11-06 1992-01-07 Brigham Young University Pyridine-containing alkoxysilanes bonded to inorganic supports and processes of using the same for removing and concentrating desired ions from solutions
AU6870091A (en) * 1989-11-08 1991-06-13 Fmc Corporation Combined centrifuge tube and porous selection means for separation and recovery of biological materials
EP0532591A4 (en) * 1990-06-04 1993-07-21 Abaxis, Inc. Analytical rotors and methods for analysis of biological fluids
US5141719A (en) * 1990-07-18 1992-08-25 Bio-Rad Laboratories, Inc. Multi-sample filtration plate assembly
US5116496A (en) * 1991-03-19 1992-05-26 Minnesota Mining And Manufacturing Company Membrane-containing wells for microtitration and microfiltration
DE69327891T2 (de) * 1992-07-06 2000-07-20 Terumo K.K., Tokio/Tokyo Material zur Entfernung von pathogener Substanz und mit diesem Material hergestellter Blutfilter
EP0588564B1 (fr) * 1992-09-18 1996-11-13 AMERSHAM INTERNATIONAL plc Dispositif et méthode de séparation par affinité
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Publication number Priority date Publication date Assignee Title
WO1999060394A1 (fr) * 1998-05-15 1999-11-25 Merck Patent Gmbh Colonne de rectification miniaturisee
WO2000055185A1 (fr) * 1999-03-15 2000-09-21 Millipore Corporation Membranes chargees en metal et liees a un ligand pour chromatographie par affinite avec un ion metallique

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JIANG Y ET AL: "On-line coupling of micro-enzyme reactor with micro-membrane chromatography for protein digestion, peptide separation, and protein identification using electrospray ionization mass spectrometry", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER SCIENCE, NL, vol. 924, no. 1-2, 27 July 2001 (2001-07-27), pages 315 - 322, XP004273955, ISSN: 0021-9673 *
RECIO, ISIDRA ET AL: "Method for the production of antibacterial peptides from biological fluid at an ionic membrane. Application to the isolation of nisin and caprine lactoferricin", LAIT (2000), 80(1), 187-195, XP009008411 *

Also Published As

Publication number Publication date
GB2372464A (en) 2002-08-28
GB2372464B (en) 2003-05-14
EP1368370A2 (fr) 2003-12-10
JP2004537033A (ja) 2004-12-09
US20040069707A1 (en) 2004-04-15
AU2002232016A1 (en) 2002-09-12
GB0104429D0 (en) 2001-04-11
WO2002068452A2 (fr) 2002-09-06
WO2002068452B1 (fr) 2004-02-19

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