WO2006023398A3 - Reactifs de spectrometrie de masse marques par des isotopes modulaires et procedes d'analyse quantitative d'acides amines, de peptides et de proteines - Google Patents

Reactifs de spectrometrie de masse marques par des isotopes modulaires et procedes d'analyse quantitative d'acides amines, de peptides et de proteines Download PDF

Info

Publication number
WO2006023398A3
WO2006023398A3 PCT/US2005/028780 US2005028780W WO2006023398A3 WO 2006023398 A3 WO2006023398 A3 WO 2006023398A3 US 2005028780 W US2005028780 W US 2005028780W WO 2006023398 A3 WO2006023398 A3 WO 2006023398A3
Authority
WO
WIPO (PCT)
Prior art keywords
mass spectrometry
methods
quantitation
peptides
modular
Prior art date
Application number
PCT/US2005/028780
Other languages
English (en)
Other versions
WO2006023398A2 (fr
Inventor
Gavin E Reid
Kade D Roberts
Original Assignee
Ludwig Inst Cancer Res
Gavin E Reid
Kade D Roberts
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2004904613A external-priority patent/AU2004904613A0/en
Application filed by Ludwig Inst Cancer Res, Gavin E Reid, Kade D Roberts filed Critical Ludwig Inst Cancer Res
Publication of WO2006023398A2 publication Critical patent/WO2006023398A2/fr
Publication of WO2006023398A3 publication Critical patent/WO2006023398A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

L'invention concerne des procédés de spectrométrie de masse en tandem pouvant être utilisés dans une analyse de protéome. L'invention concerne également des composés contenant une charge fixe marquée par des isotopes stables modulaires utilisés comme réactifs de spectrométrie de masse (MS) dans des analyses multiplex. L'invention concerne enfin des méthodes permettant de quantifier des acides aminés, des peptides et des protéines à l'aide de techniques de spectrométrie de masse en tandem.
PCT/US2005/028780 2004-08-16 2005-08-12 Reactifs de spectrometrie de masse marques par des isotopes modulaires et procedes d'analyse quantitative d'acides amines, de peptides et de proteines WO2006023398A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
AU2004904613A AU2004904613A0 (en) 2004-08-16 "Modular" isotope labelled mass spectrometry reagents and methods for quantitation of amino acids, peptides and proteins
AU2004904613 2004-08-16
US61190504P 2004-09-21 2004-09-21
US60/611,905 2004-09-21

Publications (2)

Publication Number Publication Date
WO2006023398A2 WO2006023398A2 (fr) 2006-03-02
WO2006023398A3 true WO2006023398A3 (fr) 2006-07-13

Family

ID=35429367

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/028780 WO2006023398A2 (fr) 2004-08-16 2005-08-12 Reactifs de spectrometrie de masse marques par des isotopes modulaires et procedes d'analyse quantitative d'acides amines, de peptides et de proteines

Country Status (1)

Country Link
WO (1) WO2006023398A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8067730B2 (en) 2007-07-20 2011-11-29 The George Washington University Laser ablation electrospray ionization (LAESI) for atmospheric pressure, In vivo, and imaging mass spectrometry
US8901487B2 (en) 2007-07-20 2014-12-02 George Washington University Subcellular analysis by laser ablation electrospray ionization mass spectrometry
US7964843B2 (en) 2008-07-18 2011-06-21 The George Washington University Three-dimensional molecular imaging by infrared laser ablation electrospray ionization mass spectrometry
US9202678B2 (en) 2008-11-14 2015-12-01 Board Of Trustees Of Michigan State University Ultrafast laser system for biological mass spectrometry
EP2529388B1 (fr) * 2010-01-29 2019-10-23 Micromass UK Limited Réactifs de fragmentation pour une spectrométrie de masse
US8829426B2 (en) 2011-07-14 2014-09-09 The George Washington University Plume collimation for laser ablation electrospray ionization mass spectrometry

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003025576A2 (fr) * 2001-09-14 2003-03-27 Xzillion Gmbh & Co. Kg Marqueurs de masse
WO2004046731A2 (fr) * 2002-11-18 2004-06-03 Ludwig Institute For Cancer Research Methode permettant d'analyser des acides amines, des peptides et des proteines

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003025576A2 (fr) * 2001-09-14 2003-03-27 Xzillion Gmbh & Co. Kg Marqueurs de masse
WO2004046731A2 (fr) * 2002-11-18 2004-06-03 Ludwig Institute For Cancer Research Methode permettant d'analyser des acides amines, des peptides et des proteines

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
LEAVENS WILLIAM J ET AL: "Derivatization for liquid chromatography/electrospray mass spectrometry: synthesis of tris(trimethoxyphenyl)phosphonium compounds and their derivatives of amine and carboxylic acids.", RAPID COMMUNICATIONS IN MASS SPECTROMETRY : RCM. 2002, vol. 16, no. 5, 2002, pages 433 - 441, XP009058402, ISSN: 0951-4198 *
REID G E ET AL: "Selective Identification and Quantitative Analysis of Methionine Containing Peptides by Charge Derivatization and Tandem Mass Spectrometry", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC, US, vol. 16, no. 7, July 2005 (2005-07-01), pages 1131 - 1150, XP004976154, ISSN: 1044-0305 *
ROTH K D W ET AL: "CHARGE DERIVATIZATION OF PEPTIDES FOR ANALYSIS BY MASS SPECTROMETRY", MASS SPECTROMETRY REVIEWS, JOHN WILEY AND SONS, NEW YORK, NY, US, vol. 17, no. 4, 1998, pages 255 - 274, XP000922702, ISSN: 0277-7037 *
THOMPSON A ET AL: "TANDEM MASS TAGS: A NOVEL QUANTIFICATION STRATEGY FOR COMPARATIVE ANALYSIS OF COMPLEX PROTEIN MIXTURES BY MS/MS", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY. COLUMBUS, US, vol. 75, 1 March 2003 (2003-03-01), pages 1895 - 1904, XP002328688, ISSN: 0003-2700 *

Also Published As

Publication number Publication date
WO2006023398A2 (fr) 2006-03-02

Similar Documents

Publication Publication Date Title
WO2008005455A3 (fr) Déplétion des peptides par anticorps et son application à la préparation des échantillons pour spectrométrie de masse
WO2003092581A3 (fr) Composes de capture, recueils de ceux-ci et procedes d'analyse du proteome et compositions complexes
WO2006023398A3 (fr) Reactifs de spectrometrie de masse marques par des isotopes modulaires et procedes d'analyse quantitative d'acides amines, de peptides et de proteines
WO2001075432A3 (fr) Procede et dispositif d'analyse de molecules chargees par focalisation isoelectrique d'une solution
WO2003057845A3 (fr) Systemes et procedes automatises d'analyse de modification post-translationellle proteique
WO2001094935A3 (fr) Etiquetage d'echantillons proteomiques pendant la proteolyse a des fins de quantification et d'analyse multiple d'echantillons
WO2003074992A3 (fr) Proteines phosphorylees et utilisations associees
WO2005003315A3 (fr) Compositions et methodes pour la transfection assistee par peptide
GB2387653B (en) Protein mixture analysis by mass spectrometry
WO2003065003A3 (fr) Alterations de proteine de matrice nucleaire associees au cancer du colon et au cancer du colon avec metastase au foie, et utilisations associees
ATE432939T1 (de) Verfahren und kit zur isolierung phosphorylierter peptide
WO2005028497A3 (fr) Peptides se liant a des recepteurs derives de proteines sars s
AU2003220321A1 (en) Methods for quantitative analysis by tandem mass spectrometry
PL1889070T3 (pl) Sposób określania stężenia asymetrycznej dimetyloargininy (ADMA)
AU2003237282A1 (en) Automated protein analysis system comprising capillary electrophoresis-tandem mass spectrometry
WO2005019831A3 (fr) Procedes pour la reduction de la complexite d'un echantillon au moyen d'anticorps a des sites antigeniques de petite dimension
DK1576157T3 (da) Fluorescerende proteiner fra Copepode-arter og fremgangsmåder til anvendelse af samme
WO2002059144A3 (fr) Marquage differentiel pour l'analyse de melanges proteiniques complexes
AU2174002A (en) Method for analyzing proteins
WO2003027681A3 (fr) Procede d'analyse de proteines
PL1531332T3 (pl) Analiza i typowanie białek monoklonalnych z użyciem elektroforezy kapilarnej i przemieszczenia immunologicznego
AU2002358219A1 (en) Protein analysis using mass spectrometry
WO2002037121A3 (fr) Detection d'acides amines modifies par spectrometrie de masse
WO2008125957A3 (fr) Procédé d'identification et de quantification relative de protéines cellulaires
AU2003256016A1 (en) Differential labeling for quantitative analysis of complex protein mixtures by trifunctional synthetic peptide based reagents with iodoacetamido groups

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KM KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NG NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU LV MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase