WO2002059330A3 - Artificial chromosomes comprising concatemers of expressible nucleotide sequences - Google Patents

Artificial chromosomes comprising concatemers of expressible nucleotide sequences Download PDF

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Publication number
WO2002059330A3
WO2002059330A3 PCT/DK2002/000058 DK0200058W WO02059330A3 WO 2002059330 A3 WO2002059330 A3 WO 2002059330A3 DK 0200058 W DK0200058 W DK 0200058W WO 02059330 A3 WO02059330 A3 WO 02059330A3
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WO
WIPO (PCT)
Prior art keywords
artificial chromosomes
nucleotide sequences
concatemers
expressible nucleotide
host cell
Prior art date
Application number
PCT/DK2002/000058
Other languages
French (fr)
Other versions
WO2002059330A2 (en
WO2002059330A8 (en
Inventor
Neil Goldsmith
Alexandra M P Santan Soerensen
Soeren V S Nielsen
Original Assignee
Evolva Biotech As
Neil Goldsmith
Alexandra M P Santan Soerensen
Soeren V S Nielsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evolva Biotech As, Neil Goldsmith, Alexandra M P Santan Soerensen, Soeren V S Nielsen filed Critical Evolva Biotech As
Priority to AU2002226307A priority Critical patent/AU2002226307A1/en
Publication of WO2002059330A2 publication Critical patent/WO2002059330A2/en
Publication of WO2002059330A3 publication Critical patent/WO2002059330A3/en
Publication of WO2002059330A8 publication Critical patent/WO2002059330A8/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

In the present invention is disclosed the use of artificial chromosomes for the coordinated and controllable expression of large numbers of heterologous genes in a single host cell. In particular, the invention relates to an artificial chromosome comprising at least two co-ordinatedly expressible nucleotide sequences, an artificial chromosome comprising at least two expression cassettes and a host cell comprising at least one of these artificial chromosomes as well as to a host cell comprising at least three different artificial chromosomes.
PCT/DK2002/000058 2001-01-25 2002-01-25 Artificial chromosomes comprising concatemers of expressible nucleotide sequences WO2002059330A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002226307A AU2002226307A1 (en) 2001-01-25 2002-01-25 Artificial chromosomes comprising concatemers of expressible nucleotide sequences

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200100130 2001-01-25
DKPA200100130 2001-01-25
US30086501P 2001-06-27 2001-06-27
US60/300,865 2001-06-27

Publications (3)

Publication Number Publication Date
WO2002059330A2 WO2002059330A2 (en) 2002-08-01
WO2002059330A3 true WO2002059330A3 (en) 2002-09-19
WO2002059330A8 WO2002059330A8 (en) 2004-04-15

Family

ID=26068954

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2002/000058 WO2002059330A2 (en) 2001-01-25 2002-01-25 Artificial chromosomes comprising concatemers of expressible nucleotide sequences

Country Status (2)

Country Link
AU (1) AU2002226307A1 (en)
WO (1) WO2002059330A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002059297A2 (en) 2001-01-25 2002-08-01 Evolva Biotech A/S A library of a collection of cells
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
US20060130157A1 (en) 2004-10-22 2006-06-15 Kevin Wells Ungulates with genetically modified immune systems
ES2548377T3 (en) 2008-10-27 2015-10-16 Revivicor, Inc. Immunosuppressed ungulates
CA2767724A1 (en) 2009-07-23 2011-01-27 Chromatin, Inc. Sorghum centromere sequences and minichromosomes
CN111471603B (en) * 2020-06-08 2021-06-25 广西大学 Aroma-producing pichia guilliermondii for producing beta-glucosidase and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991000920A2 (en) * 1989-07-07 1991-01-24 Unilever N.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
WO1997040183A2 (en) * 1996-04-10 1997-10-30 The Biological Research Center Of The Hungarian Academy Of Sciences Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
WO1998001573A1 (en) * 1996-07-09 1998-01-15 The Government Of The United States Of America Represented By The Secretary, Department Of Health And Human Services Transformation-associated recombination cloning
WO2000006715A1 (en) * 1998-07-27 2000-02-10 Genotypes Inc. Automatic eukaryotic artificial chromosome vector

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991000920A2 (en) * 1989-07-07 1991-01-24 Unilever N.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
WO1997040183A2 (en) * 1996-04-10 1997-10-30 The Biological Research Center Of The Hungarian Academy Of Sciences Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
WO1998001573A1 (en) * 1996-07-09 1998-01-15 The Government Of The United States Of America Represented By The Secretary, Department Of Health And Human Services Transformation-associated recombination cloning
WO2000006715A1 (en) * 1998-07-27 2000-02-10 Genotypes Inc. Automatic eukaryotic artificial chromosome vector

Also Published As

Publication number Publication date
WO2002059330A2 (en) 2002-08-01
AU2002226307A1 (en) 2002-08-06
WO2002059330A8 (en) 2004-04-15

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