WO2002000894A3 - Gene silencing vector - Google Patents

Gene silencing vector Download PDF

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Publication number
WO2002000894A3
WO2002000894A3 PCT/EP2001/007356 EP0107356W WO0200894A3 WO 2002000894 A3 WO2002000894 A3 WO 2002000894A3 EP 0107356 W EP0107356 W EP 0107356W WO 0200894 A3 WO0200894 A3 WO 0200894A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
gene silencing
relates
silencing vector
vectors
Prior art date
Application number
PCT/EP2001/007356
Other languages
French (fr)
Other versions
WO2002000894A2 (en
Inventor
Willem Broekaert
Zhongyi Zhou
Vladimir Mironov
Original Assignee
Cropdesign Nv
Willem Broekaert
Zhongyi Zhou
Vladimir Mironov
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cropdesign Nv, Willem Broekaert, Zhongyi Zhou, Vladimir Mironov filed Critical Cropdesign Nv
Priority to AU2001291656A priority Critical patent/AU2001291656A1/en
Publication of WO2002000894A2 publication Critical patent/WO2002000894A2/en
Publication of WO2002000894A3 publication Critical patent/WO2002000894A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Molecular Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention relates to gene silencing methods, more particularly to homology-dependent trans silencing methods. The invention relates to methods for efficiently downregulating any gene in a cell or organism. The invention provides for nucleic acid transgene constructs and vectors for use in said methods. The invention further relates to the use of said constructs or vectors in the production of transgenic cells and transgenic organisms.
PCT/EP2001/007356 2000-06-30 2001-06-27 Gene silencing vector WO2002000894A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001291656A AU2001291656A1 (en) 2000-06-30 2001-06-27 Gene silencing vector

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00870150 2000-06-30
EP00870150.0 2000-06-30

Publications (2)

Publication Number Publication Date
WO2002000894A2 WO2002000894A2 (en) 2002-01-03
WO2002000894A3 true WO2002000894A3 (en) 2002-07-25

Family

ID=8175778

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/007356 WO2002000894A2 (en) 2000-06-30 2001-06-27 Gene silencing vector

Country Status (2)

Country Link
AU (1) AU2001291656A1 (en)
WO (1) WO2002000894A2 (en)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002347343A1 (en) * 2001-12-12 2003-06-23 Plant Bioscience Limited Methods and means for gene silencing in plants
US7576262B2 (en) 2002-03-14 2009-08-18 Commonwealth Scientific And Industrial Research Organization Modified gene-silencing RNA and uses thereof
DE10212892A1 (en) * 2002-03-20 2003-10-09 Basf Plant Science Gmbh Constructs and methods for regulating gene expression
EP1613146B1 (en) * 2003-04-14 2013-06-19 CropDesign N.V. Plants having modified growth characteristics and method for making the same
US7672794B2 (en) 2003-06-24 2010-03-02 Expro Meters, Inc. System and method for operating a flow process
GB0323813D0 (en) * 2003-10-13 2003-11-12 Univ York Glucosyltransferases
NZ542110A (en) 2005-08-30 2008-07-31 Horticulture & Food Res Inst Compositions and methods for modulating pigment production in plants
CL2008000685A1 (en) 2007-03-08 2008-10-17 Horticulture & Food Res Inst METHOD FOR THE PRODUCTION OF A VEGETABLE CELL OR PLANT WITH INCREASED ASCORBATE THAT INCLUDES TRANSFORMING WITH A POLINUCLEOTIDE WITH ACTIVITY GDP-L-GALACTOSE GUANILTRANSPHERASE; POLINUCLEOTIDE CODIFYING SUCH ACTIVITY.
US20120124697A1 (en) 2007-08-29 2012-05-17 E.I.Du Pont De Nemours And Company Plants with altered root architecture, related constructs and methods involving genes encoding nucleoside diphosphatase kinase (ndk) polypeptides and homologs thereof
WO2009058030A2 (en) 2007-10-31 2009-05-07 The New Zealand Institute For Plant And Food Research Limited Resistance gene and uses thereof
EP2617831A3 (en) 2007-11-20 2013-08-07 E. I. du Pont de Nemours and Company Plants with altered root architecture, related constructs and methods involving genes encoding leucine rich repeat kinase (llrk) polypeptides and homologs thereof
WO2009123479A1 (en) 2008-04-03 2009-10-08 Sathish Puthigae Gene expression control in plants
NZ589505A (en) 2008-05-28 2012-04-27 Vialactia Biosciences Nz Ltd Methods and compositions for improving stress tolerance in plants
BRPI0923301A2 (en) 2008-12-01 2019-09-24 Vialactia Biosciences Nz Ltd "method and compositions for plant breeding"
WO2010114395A1 (en) 2009-04-01 2010-10-07 Vialactia Biosciences (Nz) Limited Control of gene expression in plants using a perennial ryegrass (lolium perenne l.) derived promoter
WO2010144276A1 (en) 2009-06-09 2010-12-16 E. I. Du Pont De Nemours And Company Drought tolerant plants and related constructs and methods involving genes encoding fatty acid desaturase family polypeptides
AR078829A1 (en) 2009-10-30 2011-12-07 Du Pont PLANTS AND SEEDS WITH ALTERED LEVELS OF STORAGE COMPOUND, RELATED CONSTRUCTIONS AND METHODS RELATED TO GENES THAT CODIFY SIMILAR PROTEINS TO THE BACTERIAL ALDOLASES OF CLASS II OF THE ACID 2,4- DIHYDROXI-HEPT-2-ENO-1,7-DIO-1,7
WO2011097215A2 (en) 2010-02-02 2011-08-11 E.I. Du Pont De Nemours And Company Plants with altered root architecture, related constructs and methods involving genes encoding lectin protein kinase (lpk) polypeptides and homologs thereof
EP2592945B1 (en) 2010-07-14 2018-01-17 E. I. du Pont de Nemours and Company Blended soy protein products with a combined pufa content having altered characteristics
WO2014007832A1 (en) 2012-07-03 2014-01-09 E. I. Du Pont De Nemours And Company Environmentally sustainable frying oils
BR112015009455A2 (en) 2012-10-30 2017-11-14 Agresearch Ltd isolated polynucleotide, use of a cell, plant cell, plant, plant part, propagule or progeny, modified plant dgat1 protein, its production method, animal or biofuel feedstock, and method for producing lipid
CA2889985C (en) 2012-10-30 2022-09-06 Agresearch Limited Improved acyltransferase polynucleotides, polypeptides, and methods of use
AU2013340444B2 (en) 2012-10-30 2019-08-29 Agresearch Limited Novel acyltransferase polynucleotides, polypeptides, and methods of use
CN109576241A (en) 2012-12-21 2019-04-05 新西兰植物和食品研究院有限公司 The regulation of gene expression
CA2901316A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
US10526378B2 (en) 2013-04-19 2020-01-07 Agresearch Limited Methods and materials for encapsulating proteins
WO2015023846A2 (en) 2013-08-16 2015-02-19 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
ES2937045T3 (en) 2013-09-13 2023-03-23 Pioneer Hi Bred Int Insecticidal proteins and methods of their use
MX2016010187A (en) 2014-02-07 2017-07-11 Pioneer Hi Bred Int Insecticidal proteins and methods for their use.
BR112017007932A2 (en) 2014-10-16 2018-01-23 Du Pont insecticide proteins and methods for their use
UY36386A (en) 2014-11-04 2016-04-29 Agres Ltd METHODS FOR THE IMPROVEMENT OF PLANTS
CA2966100A1 (en) 2014-11-04 2016-05-12 Agresearch Limited Methods for monocot plant improvement
US20170359965A1 (en) 2014-12-19 2017-12-21 E I Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
CN116333064A (en) 2015-05-19 2023-06-27 先锋国际良种公司 Insecticidal proteins and methods of use thereof
EP3943602A1 (en) 2015-08-06 2022-01-26 Pioneer Hi-Bred International, Inc. Plant derived insecticidal proteins and methods for their use
CN108603198A (en) 2015-12-16 2018-09-28 新西兰植物与食品研究所 Manipulate the composition and method of development of plants
US20180325119A1 (en) 2015-12-18 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3960863A1 (en) 2016-05-04 2022-03-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX2018015906A (en) 2016-07-01 2019-04-04 Pioneer Hi Bred Int Insecticidal proteins from plants and methods for their use.
US20230235352A1 (en) 2020-07-14 2023-07-27 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2024077110A2 (en) 2022-10-05 2024-04-11 Roger Paul Hellens Uorf::reporter gene fusions to select sequence changes to gene edit into uorfs to regulate ascorbate genes

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998053083A1 (en) * 1997-05-21 1998-11-26 Zeneca Limited Gene silencing
WO1999015682A2 (en) * 1997-09-22 1999-04-01 Plant Bioscience Limited Gene silencing materials and methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998053083A1 (en) * 1997-05-21 1998-11-26 Zeneca Limited Gene silencing
WO1999015682A2 (en) * 1997-09-22 1999-04-01 Plant Bioscience Limited Gene silencing materials and methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FIRE A: "RNA-triggered gene silencing", TRENDS IN GENETICS, vol. 15, no. 9, 1 September 1999 (1999-09-01), pages 358 - 363, XP004176656, ISSN: 0168-9525 *
MONTGOMERY M. K. ET AL.: "Double-stranded RNA as a mediator in sequence-specific genetic silencing and co-suppression", TRENDS IN GENETICS, vol. 14, no. 7, 1 July 1998 (1998-07-01), pages 255 - 258, XP004124680, ISSN: 0168-9525 *

Also Published As

Publication number Publication date
AU2001291656A1 (en) 2002-01-08
WO2002000894A2 (en) 2002-01-03

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