WO2002029064A3 - Method for establishing germ cell-specific gene expression - Google Patents

Method for establishing germ cell-specific gene expression Download PDF

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Publication number
WO2002029064A3
WO2002029064A3 PCT/EP2001/011354 EP0111354W WO0229064A3 WO 2002029064 A3 WO2002029064 A3 WO 2002029064A3 EP 0111354 W EP0111354 W EP 0111354W WO 0229064 A3 WO0229064 A3 WO 0229064A3
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WO
WIPO (PCT)
Prior art keywords
establishing
germ cell
gene expression
specific gene
germ
Prior art date
Application number
PCT/EP2001/011354
Other languages
French (fr)
Other versions
WO2002029064A2 (en
Inventor
Erez Raz
Original Assignee
Mermaid Pharmaceuticals Gmbh
Erez Raz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mermaid Pharmaceuticals Gmbh, Erez Raz filed Critical Mermaid Pharmaceuticals Gmbh
Priority to AU2002221638A priority Critical patent/AU2002221638A1/en
Publication of WO2002029064A2 publication Critical patent/WO2002029064A2/en
Publication of WO2002029064A3 publication Critical patent/WO2002029064A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a novel method of establishing germ cell-specific expression of nucleic acid sequences of interest in vertebrates. In particular, the invention relates to the germ cell-specific expression of marker genes, which facilitates the isolation of the germ cells and enables subsequent in vitro_ manipulations of the germ cells.
PCT/EP2001/011354 2000-10-02 2001-10-01 Method for establishing germ cell-specific gene expression WO2002029064A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002221638A AU2002221638A1 (en) 2000-10-02 2001-10-01 Method for establishing germ cell-specific gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23722800P 2000-10-02 2000-10-02
US60/237,228 2000-10-02

Publications (2)

Publication Number Publication Date
WO2002029064A2 WO2002029064A2 (en) 2002-04-11
WO2002029064A3 true WO2002029064A3 (en) 2002-11-07

Family

ID=22892861

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/011354 WO2002029064A2 (en) 2000-10-02 2001-10-01 Method for establishing germ cell-specific gene expression

Country Status (2)

Country Link
AU (1) AU2002221638A1 (en)
WO (1) WO2002029064A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006333762A (en) * 2005-06-01 2006-12-14 Mitsubishi Chemicals Corp EXPRESSION VECTOR HAVING PROMOTER SEQUENCE OF MAMMAL-DERIVED Vasa HOMOLOG GENE AND ITS USE
US8222385B2 (en) 2007-03-26 2012-07-17 National University Corporation Tokyo University of Marine Science Technology Germ cell marker using fish vasa gene
US9920300B2 (en) * 2009-10-23 2018-03-20 The Board Of Trustees Of The Leland Stanford Junior University Induction of germ cells from pluripotent cells
CN104195177B (en) * 2014-08-05 2017-06-09 南京大学 A kind of method for significantly improving Fish genomes editorial efficiency
CN106035233B (en) * 2016-06-22 2019-10-29 贵州医科大学 Transgenic zebrafish model and construction method with g6pd1303-1497 site deletion
CN113173988B (en) * 2021-04-15 2022-10-11 中国科学院水生生物研究所 Preparation method of polyclonal antibody capable of marking fish reproductive stem cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998032879A1 (en) * 1997-01-23 1998-07-30 Cornell Research Foundation, Inc. Method for separating cells
EP1161862A2 (en) * 2000-06-08 2001-12-12 Wakamatsu, Yuko See-through medaka

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998032879A1 (en) * 1997-01-23 1998-07-30 Cornell Research Foundation, Inc. Method for separating cells
EP1161862A2 (en) * 2000-06-08 2001-12-12 Wakamatsu, Yuko See-through medaka

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BERGSTEN S E ET AL: "Role for mRNA localization in translational activation but not spatial restriction of nanos RNA.", DEVELOPMENT (CAMBRIDGE, ENGLAND) ENGLAND FEB 1999, vol. 126, no. 4, February 1999 (1999-02-01), pages 659 - 669, XP002207730, ISSN: 0950-1991 *
RAZ E ET AL: "beta-lactamase as a marker for gene expression in live zebrafish embryos.", DEVELOPMENTAL BIOLOGY. UNITED STATES 15 NOV 1998, vol. 203, no. 2, 15 November 1998 (1998-11-15), pages 290 - 294, XP002207731, ISSN: 0012-1606 *
TANAKA MINORU ET AL: "Establishment of medaka (Oryzias latipes) transgenic lines with the expression of green fluorescent protein fluorescence exclusively in germ cells: A useful model to monitor germ cells in a live vertebrate.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 98, no. 5, 27 February 2001 (2001-02-27), February 27, 2001, pages 2544 - 2549, XP002207733, ISSN: 0027-8424 *
WEIDINGER GILBERT ET AL: "Identification of tissues and patterning events required for distinct steps in early migration of zebrafish primordial germ cells.", DEVELOPMENT (CAMBRIDGE), vol. 126, no. 23, December 1999 (1999-12-01), pages 5295 - 5307, XP002207729, ISSN: 0950-1991 *
WYLIE C: "Germ cells.", CURRENT OPINION IN GENETICS & DEVELOPMENT. ENGLAND AUG 2000, vol. 10, no. 4, August 2000 (2000-08-01), pages 410 - 413, XP002207732, ISSN: 0959-437X *
YOSHIZAKI GORO ET AL: "Germ cell-specific expression of green fluorescent protein in transgenic rainbow trout under control of the rainbow trout vasa-like gene promoter.", INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, vol. 44, no. 3, April 2000 (2000-04-01), pages 323 - 326, XP008006030, ISSN: 0214-6282 *

Also Published As

Publication number Publication date
AU2002221638A1 (en) 2002-04-15
WO2002029064A2 (en) 2002-04-11

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