WO2002018586A1 - Conception et construction d'un modele de transcription de synthese d'une proteine acellulaire, et methode de dilution de type discontinue utilisee dans la synthese d'une proteine de germe de ble acellulaire - Google Patents
Conception et construction d'un modele de transcription de synthese d'une proteine acellulaire, et methode de dilution de type discontinue utilisee dans la synthese d'une proteine de germe de ble acellulaire Download PDFInfo
- Publication number
- WO2002018586A1 WO2002018586A1 PCT/JP2001/007357 JP0107357W WO0218586A1 WO 2002018586 A1 WO2002018586 A1 WO 2002018586A1 JP 0107357 W JP0107357 W JP 0107357W WO 0218586 A1 WO0218586 A1 WO 0218586A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- free
- protein
- synthesizing cell
- base sequence
- design
- Prior art date
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002522493A JP4762481B2 (ja) | 2000-08-30 | 2001-08-28 | 無細胞タンパク質合成用転写鋳型の設計および構築、並びにこれを用いる希釈バッチ方式コムギ胚芽無細胞タンパク質合成法 |
AU8020201A AU8020201A (en) | 2000-08-30 | 2001-08-28 | Design and construction of transcription template for synthesizing cell-free protein, and dilution batch-type method of synthesizing cell-free wheat germ protein by using the same |
KR10-2003-7002766A KR20030031993A (ko) | 2000-08-30 | 2001-08-28 | 무세포 단백질 합성용 전사주형의 설계와 구축 및 이를이용하는 희석회분식 밀배아 무세포 단백질 합성방법 |
AU2001280202A AU2001280202C1 (en) | 2000-08-30 | 2001-08-28 | Design and construction of transcription template for synthesizing cell-free protein, and dilution batch-type method of synthesizing cell-free wheat germ protein by using the same |
US10/363,372 US7981617B2 (en) | 2000-08-30 | 2001-08-28 | Transcription template for cell-free protein synthesis and method using the same |
CA002421012A CA2421012A1 (en) | 2000-08-30 | 2001-08-28 | Designing and construction of transcription template for cell-free protein synthesis, and dilution batch wheat embryo cell-free protein synthesis method using the same |
EP01958551A EP1314781A4 (en) | 2000-08-30 | 2001-08-28 | DESIGN AND CONSTRUCTION OF A TRANSCRIPTION MODEL OF ACELLULAR PROTEIN SYNTHESIS, AND DISCONTINUOUS DILUTION METHOD FOR USE IN SYNTHESIS OF ACELLULAR WHEAT GERM PROTEIN |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2000-261638 | 2000-08-30 | ||
JP2000261638 | 2000-08-30 | ||
JP2001-058404 | 2001-03-02 | ||
JP2001058404 | 2001-03-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2002018586A1 true WO2002018586A1 (fr) | 2002-03-07 |
Family
ID=26598840
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2001/007357 WO2002018586A1 (fr) | 2000-08-30 | 2001-08-28 | Conception et construction d'un modele de transcription de synthese d'une proteine acellulaire, et methode de dilution de type discontinue utilisee dans la synthese d'une proteine de germe de ble acellulaire |
Country Status (8)
Country | Link |
---|---|
US (1) | US7981617B2 (ja) |
EP (1) | EP1314781A4 (ja) |
JP (1) | JP4762481B2 (ja) |
KR (1) | KR20030031993A (ja) |
CN (1) | CN1252261C (ja) |
AU (1) | AU8020201A (ja) |
CA (1) | CA2421012A1 (ja) |
WO (1) | WO2002018586A1 (ja) |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004070047A1 (ja) * | 2003-02-10 | 2004-08-19 | Cellfree Sciences Co., Ltd. | 自動蛋白質合成方法及びそれを行うための装置 |
WO2006051901A1 (ja) * | 2004-11-12 | 2006-05-18 | Cellfree Sciences Co., Ltd. | 無細胞タンパク質合成方法 |
JPWO2004097014A1 (ja) * | 2003-04-25 | 2006-07-13 | 株式会社セルフリーサイエンス | ハイスループット合成システム及び該システムを自動で行うための合成装置 |
US7195895B2 (en) | 2001-07-02 | 2007-03-27 | Riken | Method of producing template DNA and method of producing protein in cell-free protein synthesis system using the same |
US8226976B2 (en) | 2007-11-05 | 2012-07-24 | Riken | Method of using cell-free protein synthesis to produce a membrane protein |
US8445232B2 (en) | 2004-11-17 | 2013-05-21 | Riken | Cell-free system for synthesis of proteins derived from cultured mammalian cells |
US8603774B2 (en) | 2002-11-28 | 2013-12-10 | National Food Research Institute | Extract of E. coli cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract |
US8664355B2 (en) | 2004-11-19 | 2014-03-04 | Riken | Cell-free protein synthesis method with the use of linear template DNA and cell extract therefor |
JP2014524244A (ja) * | 2011-08-03 | 2014-09-22 | プロセッタ アンティヴァイラル インコーポレイテッド | 化合物のスクリーニングのための無細胞翻訳系及び関連用途 |
US11618777B2 (en) | 2015-07-31 | 2023-04-04 | Shigeyuki Yokoyama | Method of manufacturing membrane protein and utilization thereof |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007528718A (ja) * | 2003-08-07 | 2007-10-18 | 株式会社セルフリーサイエンス | タンパク質チップ作製用試薬 |
JP4590249B2 (ja) * | 2004-11-17 | 2010-12-01 | 独立行政法人理化学研究所 | 糖タンパク質合成用の無細胞タンパク質合成システム |
KR100784164B1 (ko) | 2006-05-08 | 2007-12-10 | 고려대학교 산학협력단 | 이중 마커를 이용한 siRNA의 정량용 조성물 |
KR100966655B1 (ko) * | 2007-08-30 | 2010-06-30 | (주)차바이오앤디오스텍 | 무세포 단백질 합성을 이용한 트랜스아미나제의 활성스크리닝 방법 및 키트 |
US20110236941A1 (en) * | 2010-10-22 | 2011-09-29 | Lanzatech New Zealand Limited | Recombinant microorganism and methods of production thereof |
CN107629119B (zh) * | 2016-07-18 | 2021-05-14 | 河南工业大学 | 一种麦胚抗肿瘤活性蛋白的修饰方法 |
EP3354736B1 (en) * | 2017-01-25 | 2020-07-01 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Promoter construct for cell-free protein synthesis |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1987007644A1 (en) * | 1986-06-04 | 1987-12-17 | Diatech Limited | TRANSLATION OF mRNA |
JPH07203984A (ja) * | 1994-01-26 | 1995-08-08 | Yaeta Endou | 蛋白質の合成方法 |
JPH1156363A (ja) * | 1997-08-19 | 1999-03-02 | Nippon Flour Mills Co Ltd | タンパクの製造方法 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69023773T2 (de) * | 1989-07-31 | 1996-10-24 | Inst Belka Akad Nauk Sssr | Verfahren zur herstellung von polypeptiden in einem zellfreien translationssystem. |
US5747641A (en) * | 1989-12-21 | 1998-05-05 | Biogen Inc | Tat-derived transport polypeptide conjugates |
EP0972838B1 (en) | 1998-07-15 | 2004-09-15 | Roche Diagnostics GmbH | Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy |
EP1126879A4 (en) * | 1998-08-18 | 2004-08-04 | Einstein Coll Med | GENE THERAPY FOR REGULATING THE SMOOTH MUSCLE TONUS |
JP4486187B2 (ja) * | 1998-10-27 | 2010-06-23 | 武田薬品工業株式会社 | 新規g蛋白質共役型レセプター蛋白質、そのdnaおよびそのリガンド |
JP4824236B2 (ja) * | 1999-07-09 | 2011-11-30 | ジェン−プローブ・インコーポレーテッド | 核酸増幅によるhiv−1の検出 |
US6303337B1 (en) * | 1999-08-25 | 2001-10-16 | Amber Gen. Inc. | N-terminal and C-terminal markers in nascent proteins |
WO2001027260A1 (fr) | 1999-10-15 | 2001-04-19 | Wakenyaku Co., Ltd. | Molecule matricielle presentant un vaste champ d'applications et dispositif hautement efficace de synthese acellulaire de proteines utilisant cette molecule |
CA2416684A1 (en) * | 2000-07-21 | 2002-01-31 | Yaeta Endo | General means of labeling protein by using wheat embryo cell-free protein synthesis system |
ATE368747T1 (de) * | 2000-08-29 | 2007-08-15 | Cellfree Sciences Co Ltd | Verfahren zur synthese von zellfreien proteinen |
-
2001
- 2001-08-28 CN CNB018148093A patent/CN1252261C/zh not_active Expired - Fee Related
- 2001-08-28 JP JP2002522493A patent/JP4762481B2/ja not_active Expired - Fee Related
- 2001-08-28 EP EP01958551A patent/EP1314781A4/en not_active Withdrawn
- 2001-08-28 US US10/363,372 patent/US7981617B2/en not_active Expired - Fee Related
- 2001-08-28 AU AU8020201A patent/AU8020201A/xx active Pending
- 2001-08-28 KR KR10-2003-7002766A patent/KR20030031993A/ko not_active Application Discontinuation
- 2001-08-28 WO PCT/JP2001/007357 patent/WO2002018586A1/ja not_active Application Discontinuation
- 2001-08-28 CA CA002421012A patent/CA2421012A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1987007644A1 (en) * | 1986-06-04 | 1987-12-17 | Diatech Limited | TRANSLATION OF mRNA |
JPH07203984A (ja) * | 1994-01-26 | 1995-08-08 | Yaeta Endou | 蛋白質の合成方法 |
JPH1156363A (ja) * | 1997-08-19 | 1999-03-02 | Nippon Flour Mills Co Ltd | タンパクの製造方法 |
Non-Patent Citations (3)
Title |
---|
MADIN K. ET AL.: "A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plantsapparently contain a suicide system directed at ribosomes", PROC. NATL. ACAD. SCI. USA, vol. 97, no. 2, 18 January 2000 (2000-01-18), pages 559 - 564, XP002906354 * |
See also references of EP1314781A4 * |
YAETA ENDOU: "Komugi haiga wo mochiita mu-saibou tanpakushitsu housei-hou", BIO INDUSTRY, vol. 17, no. 5, May 2000 (2000-05-01), pages 20 - 27, XP002906355 * |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7846694B2 (en) | 2001-07-02 | 2010-12-07 | Riken | Process for producing template DNA and process for producing protein in cell-free protein synthesis system with the use of the same |
US7195895B2 (en) | 2001-07-02 | 2007-03-27 | Riken | Method of producing template DNA and method of producing protein in cell-free protein synthesis system using the same |
US8603774B2 (en) | 2002-11-28 | 2013-12-10 | National Food Research Institute | Extract of E. coli cells having mutation in ribosomal protein S12, and method for producing protein in cell-free system using the extract |
JPWO2004070047A1 (ja) * | 2003-02-10 | 2006-05-25 | 株式会社セルフリーサイエンス | 自動蛋白質合成方法及びそれを行うための装置 |
WO2004070047A1 (ja) * | 2003-02-10 | 2004-08-19 | Cellfree Sciences Co., Ltd. | 自動蛋白質合成方法及びそれを行うための装置 |
JPWO2004097014A1 (ja) * | 2003-04-25 | 2006-07-13 | 株式会社セルフリーサイエンス | ハイスループット合成システム及び該システムを自動で行うための合成装置 |
JP4643444B2 (ja) * | 2003-04-25 | 2011-03-02 | 株式会社セルフリーサイエンス | ハイスループット合成システム及び該システムを自動で行うための合成装置 |
WO2006051901A1 (ja) * | 2004-11-12 | 2006-05-18 | Cellfree Sciences Co., Ltd. | 無細胞タンパク質合成方法 |
US8445232B2 (en) | 2004-11-17 | 2013-05-21 | Riken | Cell-free system for synthesis of proteins derived from cultured mammalian cells |
US8664355B2 (en) | 2004-11-19 | 2014-03-04 | Riken | Cell-free protein synthesis method with the use of linear template DNA and cell extract therefor |
US8226976B2 (en) | 2007-11-05 | 2012-07-24 | Riken | Method of using cell-free protein synthesis to produce a membrane protein |
US10036755B2 (en) | 2011-04-26 | 2018-07-31 | Prosetta Antiviral, Inc. | Multiprotein assemblies |
JP2014524244A (ja) * | 2011-08-03 | 2014-09-22 | プロセッタ アンティヴァイラル インコーポレイテッド | 化合物のスクリーニングのための無細胞翻訳系及び関連用途 |
JP2017018136A (ja) * | 2011-08-03 | 2017-01-26 | プロセッタ アンティヴァイラル インコーポレイテッド | 化合物のスクリーニングのための無細胞翻訳系及び関連用途 |
US11618777B2 (en) | 2015-07-31 | 2023-04-04 | Shigeyuki Yokoyama | Method of manufacturing membrane protein and utilization thereof |
Also Published As
Publication number | Publication date |
---|---|
KR20030031993A (ko) | 2003-04-23 |
AU8020201A (en) | 2002-03-13 |
US7981617B2 (en) | 2011-07-19 |
AU2001280202B2 (en) | 2006-11-09 |
CA2421012A1 (en) | 2003-02-26 |
CN1252261C (zh) | 2006-04-19 |
EP1314781A1 (en) | 2003-05-28 |
JP4762481B2 (ja) | 2011-08-31 |
CN1449444A (zh) | 2003-10-15 |
US20040121346A1 (en) | 2004-06-24 |
EP1314781A4 (en) | 2004-11-03 |
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