WO2002004686A3 - Detection de la cytosine methylee dans des polynucleotides - Google Patents

Detection de la cytosine methylee dans des polynucleotides Download PDF

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Publication number
WO2002004686A3
WO2002004686A3 PCT/US2001/041321 US0141321W WO0204686A3 WO 2002004686 A3 WO2002004686 A3 WO 2002004686A3 US 0141321 W US0141321 W US 0141321W WO 0204686 A3 WO0204686 A3 WO 0204686A3
Authority
WO
WIPO (PCT)
Prior art keywords
mass
polynucleotide
cytosine
polynucleotides
methylated cytosine
Prior art date
Application number
PCT/US2001/041321
Other languages
English (en)
Other versions
WO2002004686A2 (fr
Inventor
Norbert O Reich
Alec M Wodtke
Original Assignee
Epigenx Pharmaceutical Inc
Norbert O Reich
Alec M Wodtke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenx Pharmaceutical Inc, Norbert O Reich, Alec M Wodtke filed Critical Epigenx Pharmaceutical Inc
Priority to AU2001281311A priority Critical patent/AU2001281311A1/en
Publication of WO2002004686A2 publication Critical patent/WO2002004686A2/fr
Publication of WO2002004686A3 publication Critical patent/WO2002004686A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

L'invention permet de déterminer le statut de méthylation des bases de cytosine dans un polynucléotide avec un degré de sensibilité plus élevé, grâce à la mise en contact du polynucléotide avec un agent qui modifie la cytosine méthylée ou non méthylée, l'amplification du polynucléotide modifié au moyen d'un ou plusieurs nucléotides 'poids lourds' puis la détermination de la masse du produit amplifié. La masse du produit amplifié est déterminée, par exemple par la spectrométrie de masse, et mesurée par rapport à la masse d'un échantillon de contrôle ayant une séquence de base identique à celle de la séquence nucléotidique étudiée. La présence ou l'absence d'une différence de masse indique la présence ou l'absence de la méthylation du polynucléotide.
PCT/US2001/041321 2000-07-10 2001-07-10 Detection de la cytosine methylee dans des polynucleotides WO2002004686A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001281311A AU2001281311A1 (en) 2000-07-10 2001-07-10 Detecting methylated cytosine in polynucleotides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US21705900P 2000-07-10 2000-07-10
US60/217,059 2000-07-10
US25955901P 2001-01-02 2001-01-02
US60/259,559 2001-01-02

Publications (2)

Publication Number Publication Date
WO2002004686A2 WO2002004686A2 (fr) 2002-01-17
WO2002004686A3 true WO2002004686A3 (fr) 2003-09-18

Family

ID=26911575

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/041321 WO2002004686A2 (fr) 2000-07-10 2001-07-10 Detection de la cytosine methylee dans des polynucleotides

Country Status (2)

Country Link
AU (1) AU2001281311A1 (fr)
WO (1) WO2002004686A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10132212B4 (de) * 2001-06-27 2005-11-24 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung durch vergleichende Analyse der Einzelstränge von Amplifikaten
JP2006500901A (ja) * 2001-09-26 2006-01-12 エピジェンクス ファーマスーティカルズ、 インコーポレイテッド Ｄｎａメチル化変化のアッセイ
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
CN102982253B (zh) * 2011-09-02 2015-11-25 深圳华大基因科技服务有限公司 一种多样本间甲基化差异检测方法及装置
CN113249362B (zh) * 2020-02-07 2023-04-14 辉大(上海)生物科技有限公司 经改造的胞嘧啶碱基编辑器及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19905082C1 (de) * 1999-01-29 2000-05-18 Epigenomics Gmbh Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben
WO2002000705A2 (fr) * 2000-06-30 2002-01-03 Epigenomics Ag Procede et acides nucleiques pour la differenciation de cellules tumorales d'astrocytomes, d'oligoastrocytomes et d'oligodendrogliomes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19905082C1 (de) * 1999-01-29 2000-05-18 Epigenomics Gmbh Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben
WO2002000705A2 (fr) * 2000-06-30 2002-01-03 Epigenomics Ag Procede et acides nucleiques pour la differenciation de cellules tumorales d'astrocytomes, d'oligoastrocytomes et d'oligodendrogliomes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KRIEG A M: "THE CPG MOTIF: IMPLICATIONS FOR CLINICAL IMMUNOLOGY", BIODRUGS, AUCKLAND, NZ, vol. 10, no. 5, 1998, pages 341 - 346, XP000949909, ISSN: 1173-8804 *
PAUL C L ET AL: "Cytosine methylation: quantitation by automated genomic sequencing and GENESCAN analysis", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 21, no. 1, July 1996 (1996-07-01), pages 126 - 133, XP002143107, ISSN: 0736-6205 *
REIN ET AL: "Identifying 5-methylcytosine and related modifications in DNA genomes", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 10, 1998, pages 2255 - 2264, XP002143106, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
AU2001281311A1 (en) 2002-01-21
WO2002004686A2 (fr) 2002-01-17

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