WO2002004686A3 - Detection de la cytosine methylee dans des polynucleotides - Google Patents
Detection de la cytosine methylee dans des polynucleotides Download PDFInfo
- Publication number
- WO2002004686A3 WO2002004686A3 PCT/US2001/041321 US0141321W WO0204686A3 WO 2002004686 A3 WO2002004686 A3 WO 2002004686A3 US 0141321 W US0141321 W US 0141321W WO 0204686 A3 WO0204686 A3 WO 0204686A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- mass
- polynucleotide
- cytosine
- polynucleotides
- methylated cytosine
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2001281311A AU2001281311A1 (en) | 2000-07-10 | 2001-07-10 | Detecting methylated cytosine in polynucleotides |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US21705900P | 2000-07-10 | 2000-07-10 | |
US60/217,059 | 2000-07-10 | ||
US25955901P | 2001-01-02 | 2001-01-02 | |
US60/259,559 | 2001-01-02 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002004686A2 WO2002004686A2 (fr) | 2002-01-17 |
WO2002004686A3 true WO2002004686A3 (fr) | 2003-09-18 |
Family
ID=26911575
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2001/041321 WO2002004686A2 (fr) | 2000-07-10 | 2001-07-10 | Detection de la cytosine methylee dans des polynucleotides |
Country Status (2)
Country | Link |
---|---|
AU (1) | AU2001281311A1 (fr) |
WO (1) | WO2002004686A2 (fr) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10132212B4 (de) * | 2001-06-27 | 2005-11-24 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierung durch vergleichende Analyse der Einzelstränge von Amplifikaten |
JP2006500901A (ja) * | 2001-09-26 | 2006-01-12 | エピジェンクス ファーマスーティカルズ、 インコーポレイテッド | Dnaメチル化変化のアッセイ |
US20110151438A9 (en) | 2001-11-19 | 2011-06-23 | Affymetrix, Inc. | Methods of Analysis of Methylation |
US7901882B2 (en) | 2006-03-31 | 2011-03-08 | Affymetrix, Inc. | Analysis of methylation using nucleic acid arrays |
CN102982253B (zh) * | 2011-09-02 | 2015-11-25 | 深圳华大基因科技服务有限公司 | 一种多样本间甲基化差异检测方法及装置 |
CN113249362B (zh) * | 2020-02-07 | 2023-04-14 | 辉大(上海)生物科技有限公司 | 经改造的胞嘧啶碱基编辑器及其应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19905082C1 (de) * | 1999-01-29 | 2000-05-18 | Epigenomics Gmbh | Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben |
WO2002000705A2 (fr) * | 2000-06-30 | 2002-01-03 | Epigenomics Ag | Procede et acides nucleiques pour la differenciation de cellules tumorales d'astrocytomes, d'oligoastrocytomes et d'oligodendrogliomes |
-
2001
- 2001-07-10 WO PCT/US2001/041321 patent/WO2002004686A2/fr active Application Filing
- 2001-07-10 AU AU2001281311A patent/AU2001281311A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19905082C1 (de) * | 1999-01-29 | 2000-05-18 | Epigenomics Gmbh | Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben |
WO2002000705A2 (fr) * | 2000-06-30 | 2002-01-03 | Epigenomics Ag | Procede et acides nucleiques pour la differenciation de cellules tumorales d'astrocytomes, d'oligoastrocytomes et d'oligodendrogliomes |
Non-Patent Citations (3)
Title |
---|
KRIEG A M: "THE CPG MOTIF: IMPLICATIONS FOR CLINICAL IMMUNOLOGY", BIODRUGS, AUCKLAND, NZ, vol. 10, no. 5, 1998, pages 341 - 346, XP000949909, ISSN: 1173-8804 * |
PAUL C L ET AL: "Cytosine methylation: quantitation by automated genomic sequencing and GENESCAN analysis", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 21, no. 1, July 1996 (1996-07-01), pages 126 - 133, XP002143107, ISSN: 0736-6205 * |
REIN ET AL: "Identifying 5-methylcytosine and related modifications in DNA genomes", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 26, no. 10, 1998, pages 2255 - 2264, XP002143106, ISSN: 0305-1048 * |
Also Published As
Publication number | Publication date |
---|---|
AU2001281311A1 (en) | 2002-01-21 |
WO2002004686A2 (fr) | 2002-01-17 |
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