ES2191666T3 - Metodos de alargamiento de cebo de nucleotido simple para detectar alelos especificos y kit apropiado para ello. - Google Patents
Metodos de alargamiento de cebo de nucleotido simple para detectar alelos especificos y kit apropiado para ello.Info
- Publication number
- ES2191666T3 ES2191666T3 ES93918149T ES93918149T ES2191666T3 ES 2191666 T3 ES2191666 T3 ES 2191666T3 ES 93918149 T ES93918149 T ES 93918149T ES 93918149 T ES93918149 T ES 93918149T ES 2191666 T3 ES2191666 T3 ES 2191666T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleotide
- cebing
- alelos
- detect specific
- extension methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4712—Cystic fibrosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Pulmonology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
SE DESCRIBE UN METODO PARA DETERMINAR LA IDENTIDAD DE UNA BASE NUCLEOTIDA EN UNA POSICION ESPECIFICA DE UN ACIDO NUCLEICO DE INTERES. LA IDENTIDAD DEL NUCLEOTIDO SE BASA EN SI SE PUEDE EXTENDER O NO UN IMPRIMADOR EN PRESENCIA DE UN NUCLEOTIDO DE UN SUSTRATO ESPECIFICO. LA BASE NUCLEOTIDA A DETERMINAR SE ENCUENTRA EN EL EXTREMO 3'' INMEDIATAMENTE ADYACENTE A LA NUCLEOTIDO FINAL 3'' DEL IMPRIMADOR. EL IMPRIMADOR SE EXTENDERA SI EL NUCLEOTIDO DEL SUSTRATO ES COMPLEMENTARIO A LA BASE NUCLEOTIDA DEL ACIDO NUCLEICO DE INTERES Y NO LO HARA EN CASO DE NO EXISTIR TAL COMPLEMENTARIEDAD. EL METODO UTILIZADO PARA DETERMINAR UN GEN NORMAL Y MUTADO SE ILUSTRA EN LA FIGURA.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL10238292A IL102382A (en) | 1992-07-02 | 1992-07-02 | Method for detecting nucleotide sequences and test kit therefor |
US91987292A | 1992-07-27 | 1992-07-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2191666T3 true ES2191666T3 (es) | 2003-09-16 |
Family
ID=26322472
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES93918149T Expired - Lifetime ES2191666T3 (es) | 1992-07-02 | 1993-07-01 | Metodos de alargamiento de cebo de nucleotido simple para detectar alelos especificos y kit apropiado para ello. |
Country Status (9)
Country | Link |
---|---|
EP (1) | EP0648222B1 (es) |
JP (1) | JPH08507198A (es) |
AT (1) | ATE231881T1 (es) |
AU (1) | AU4770393A (es) |
CA (1) | CA2139433A1 (es) |
DE (1) | DE69332666T2 (es) |
ES (1) | ES2191666T3 (es) |
FI (1) | FI946127A (es) |
WO (1) | WO1994001447A1 (es) |
Families Citing this family (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19515552A1 (de) * | 1995-04-27 | 1996-10-31 | Europ Lab Molekularbiolog | Simultane Sequenzierung von Nukleinsäuren |
US5882867A (en) * | 1995-06-07 | 1999-03-16 | Dade Behring Marburg Gmbh | Detection of nucleic acids by formation of template-dependent product |
US5876936A (en) * | 1997-01-15 | 1999-03-02 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators |
US6073209A (en) * | 1997-03-31 | 2000-06-06 | Ark Research Corporation | Data storage controller providing multiple hosts with access to multiple storage subsystems |
US6268136B1 (en) | 1997-06-16 | 2001-07-31 | Exact Science Corporation | Methods for stool sample preparation |
CA2245039A1 (en) | 1998-08-13 | 2000-02-13 | Vito Scalia | Primer-specific and mispair extension assay for identifying gene variation |
EP1131468A2 (en) * | 1998-11-23 | 2001-09-12 | Exact Sciences Corporation | Primer extension methods utilizing donor and acceptor molecules for detecting nucleic acids |
US20020048752A1 (en) * | 1998-11-23 | 2002-04-25 | Stanley N. Lapidus | Methods for detecting lower-frequency molecules |
US6586177B1 (en) | 1999-09-08 | 2003-07-01 | Exact Sciences Corporation | Methods for disease detection |
JP3565752B2 (ja) * | 1999-11-02 | 2004-09-15 | 株式会社海洋バイオテクノロジー研究所 | Dnaジャイレース遺伝子中に存在する特徴的な塩基配列を用いた遅発育性マイコバクテリアの同定法及び特異的検出法 |
WO2001042510A2 (en) * | 1999-12-10 | 2001-06-14 | Trustees Of Princeton University | Method for selectively isolating a nucleic acid |
CN100343389C (zh) * | 2002-11-07 | 2007-10-17 | 松原洋一 | 基因突变检出法 |
GB0406864D0 (en) * | 2004-03-26 | 2004-04-28 | Qiagen As | Nucleic acid sequencing |
EP1784508B1 (en) | 2004-08-09 | 2012-10-03 | Generation Biotech, LLC | Method for nucleic acid isolation and amplification |
US9109256B2 (en) | 2004-10-27 | 2015-08-18 | Esoterix Genetic Laboratories, Llc | Method for monitoring disease progression or recurrence |
US9777314B2 (en) | 2005-04-21 | 2017-10-03 | Esoterix Genetic Laboratories, Llc | Analysis of heterogeneous nucleic acid samples |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2136059T5 (es) * | 1989-02-13 | 2005-02-16 | Geneco Pty. Ltd. | Deteccion de una secuencia de acidos nucleicos o de un cambio en la misma. |
FR2650840B1 (fr) * | 1989-08-11 | 1991-11-29 | Bertin & Cie | Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications |
US5137806A (en) * | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
IL97222A (en) * | 1990-02-16 | 1995-08-31 | Orion Yhtymae Oy | Method and Responder for Determining Special Changes to Nucleotide |
US6004744A (en) * | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
WO1992016657A1 (en) * | 1991-03-13 | 1992-10-01 | E.I. Du Pont De Nemours And Company | Method of identifying a nucleotide present at a defined position in a nucleic acid |
-
1993
- 1993-07-01 JP JP6503466A patent/JPH08507198A/ja not_active Withdrawn
- 1993-07-01 AU AU47703/93A patent/AU4770393A/en not_active Abandoned
- 1993-07-01 ES ES93918149T patent/ES2191666T3/es not_active Expired - Lifetime
- 1993-07-01 WO PCT/US1993/006364 patent/WO1994001447A1/en active IP Right Grant
- 1993-07-01 CA CA002139433A patent/CA2139433A1/en not_active Abandoned
- 1993-07-01 DE DE69332666T patent/DE69332666T2/de not_active Expired - Fee Related
- 1993-07-01 EP EP93918149A patent/EP0648222B1/en not_active Expired - Lifetime
- 1993-07-01 AT AT93918149T patent/ATE231881T1/de not_active IP Right Cessation
-
1994
- 1994-12-28 FI FI946127A patent/FI946127A/fi not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP0648222A1 (en) | 1995-04-19 |
ATE231881T1 (de) | 2003-02-15 |
CA2139433A1 (en) | 1994-01-20 |
JPH08507198A (ja) | 1996-08-06 |
FI946127A (fi) | 1995-02-20 |
DE69332666T2 (de) | 2003-12-18 |
FI946127A0 (fi) | 1994-12-28 |
EP0648222A4 (en) | 1999-03-17 |
AU4770393A (en) | 1994-01-31 |
EP0648222B1 (en) | 2003-01-29 |
WO1994001447A1 (en) | 1994-01-20 |
DE69332666D1 (de) | 2003-03-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2191666T3 (es) | Metodos de alargamiento de cebo de nucleotido simple para detectar alelos especificos y kit apropiado para ello. | |
AU2001245945A1 (en) | Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof | |
ES2147180T3 (es) | Procedimiento para la deteccion de acidos nucleicos semejantes. | |
CA2071537A1 (en) | Method and reagent for determining specific nucleotide variations | |
DK0800587T3 (da) | Komparativ hybridisering af nukleinsyrerækker | |
DE69936532D1 (de) | Verfahren zum quantitativen nachweis von nukleinsäuren | |
DE3578190D1 (de) | Test fuer nukleinsaeuresequenzen, insbesondere genetische veraenderungen. | |
WO2003000920A3 (en) | Methods for characterization of nucleic acid molecules | |
CA2172248A1 (en) | Self-sustained sequence replication electrochemiluminescent nucleic acid assay | |
WO2002018632A3 (de) | Verfahren zur bestimmung des methylierungsgrades von bestimmten cytosinen in genomischer dna im sequenzkontext 5'-cpg-3' | |
CA2189941A1 (en) | Fluorescence Polarization Detection of Nucleic Acids | |
WO2002061133A3 (en) | Nucleic acid detection using structured probes | |
WO1996009386A3 (en) | Allelic variation of the serotonin 5ht2c receptor | |
EP0915174A4 (en) | METHOD FOR DETECTION OF TARGET NUCLEOTIDE SEQUENCES | |
ES2170222T3 (es) | Derivados c-nucleosidos y su utilizacion en la deteccion de acidos nucleicos. | |
ES2018916A6 (es) | Un metodo de ensayo de hibridacion de acidos nucleicos. | |
EP0272098A3 (en) | Method for amplification and detection of rna sequences | |
EP0803578A3 (en) | Kit for amplification and detection of nucleic acid of cytomegalovirus (cmv) utilizing a nucleic acid sequence (ss2.7) | |
AU1728001A (en) | Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample | |
EP0128018A3 (en) | Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe | |
WO1998044157A3 (en) | Methods for identifying variations in polynucleotide sequences | |
SG153624A1 (en) | Isometric primer extension method and kit for detection and quantification of specific nucleic acid | |
WO1999024612A3 (en) | Multiple sequential polynucleotide displacement reactions for signal amplification and processing | |
WO1998026097A3 (en) | NUCLEIC ACID PRIMERS AND PROBES FOR DETECTING $i(LEGIONELLA PNEUMOPHILA) | |
WO2001016374A3 (en) | Methods and reagents for in situ amplification |