WO2001098453A3 - Vecteurs d'expression comprenant un fragment modifie de l'operon tryptophane - Google Patents
Vecteurs d'expression comprenant un fragment modifie de l'operon tryptophane Download PDFInfo
- Publication number
- WO2001098453A3 WO2001098453A3 PCT/FR2001/001952 FR0101952W WO0198453A3 WO 2001098453 A3 WO2001098453 A3 WO 2001098453A3 FR 0101952 W FR0101952 W FR 0101952W WO 0198453 A3 WO0198453 A3 WO 0198453A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- recombinant protein
- initiation codon
- construct
- interest
- downstream
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 5
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 5
- 108091081024 Start codon Proteins 0.000 title abstract 2
- 230000002018 overexpression Effects 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 abstract 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 210000001236 prokaryotic cell Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU69224/01A AU6922401A (en) | 2000-06-22 | 2001-06-21 | Modified construct downstream of the initiation codon for recombinant protein overexpression |
MXPA02012880A MXPA02012880A (es) | 2000-06-22 | 2001-06-21 | Construccion modificada hacia el extremo 3' del codon de iniciacion para sobreexpresion de proteina recombinante. |
CA002413612A CA2413612A1 (fr) | 2000-06-22 | 2001-06-21 | Vecteurs d'expression comprenant un fragment modifie de l'operon tryptophane |
US10/311,976 US20040260060A1 (en) | 2000-06-22 | 2001-06-21 | Constructs modified downstreams of the initiation codon for recombinant protein |
JP2002504602A JP2004500875A (ja) | 2000-06-22 | 2001-06-21 | 組換えタンパク質過剰発現用開始コドン下流の修飾構築体 |
BR0111907-9A BR0111907A (pt) | 2000-06-22 | 2001-06-21 | Construção para a expressão de um gene, vetor, célula hospedeira, processo de produção de uma proteìna recombinante de interesse em uma célula hospedeira e utilização de uma construção e de uma proteìna recombinante |
EP01947565A EP1315822A2 (fr) | 2000-06-22 | 2001-06-21 | Vecteurs d'expression comprenant un fragment modifie de l'operon tryptophane |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR00/08002 | 2000-06-22 | ||
FR0008002A FR2810675B1 (fr) | 2000-06-22 | 2000-06-22 | Construction modifiee en aval du codon d'initiation pour la surexpression de proteines recombinantes |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2001098453A2 WO2001098453A2 (fr) | 2001-12-27 |
WO2001098453A3 true WO2001098453A3 (fr) | 2002-08-01 |
Family
ID=8851556
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/FR2001/001952 WO2001098453A2 (fr) | 2000-06-22 | 2001-06-21 | Vecteurs d'expression comprenant un fragment modifie de l'operon tryptophane |
Country Status (10)
Country | Link |
---|---|
US (1) | US20040260060A1 (fr) |
EP (1) | EP1315822A2 (fr) |
JP (1) | JP2004500875A (fr) |
CN (1) | CN1443242A (fr) |
AU (1) | AU6922401A (fr) |
BR (1) | BR0111907A (fr) |
CA (1) | CA2413612A1 (fr) |
FR (1) | FR2810675B1 (fr) |
MX (1) | MXPA02012880A (fr) |
WO (1) | WO2001098453A2 (fr) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1833970A2 (fr) * | 2004-12-22 | 2007-09-19 | Genentech, Inc. | Procedes pour la production d'une pluralite de proteines transmembranaires solubles |
AU2008283991A1 (en) * | 2007-08-03 | 2009-02-12 | Pfenex, Inc. | Translation initiation region sequences for the optimal expression of heterologous proteins |
KR100961565B1 (ko) * | 2008-03-10 | 2010-06-07 | 충남대학교산학협력단 | 목적하는 단백질 발현 수준을 제공하는 초기 코돈의발굴방법, 및 이를 이용한 재조합 단백질의 발현 조절 방법및 생산 방법 |
US20110129873A1 (en) * | 2008-04-30 | 2011-06-02 | Monsanto Technology Llc | Recombinant DNA Vectors for Expression of Human Prolactin Antagonists |
CA2729839A1 (fr) * | 2008-07-03 | 2010-01-07 | Diane Retallack | Procede de criblage a haut debit et son utilisation pour identifier une plateforme de production d'une proteine de liaison multifonctionnelle |
CN110491447B (zh) * | 2019-08-05 | 2021-08-17 | 浙江省农业科学院 | 一种用于异源基因体外表达的密码子优化方法及应用 |
CN115960934A (zh) * | 2022-08-24 | 2023-04-14 | 深圳柏垠生物科技有限公司 | 大肠杆菌表达外源基因优化方法及其序列 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4738921A (en) * | 1984-09-27 | 1988-04-19 | Eli Lilly And Company | Derivative of the tryptophan operon for expression of fused gene products |
WO1998045427A2 (fr) * | 1997-04-04 | 1998-10-15 | Genentech, Inc. | Molecules agonistes du facteur de croissance de l'insulinoide |
WO1999007735A2 (fr) * | 1997-08-07 | 1999-02-18 | Creative Peptides Sweden Ab | Expression recombinee du peptide c de l'insuline |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4772555A (en) * | 1985-03-27 | 1988-09-20 | Genentech, Inc. | Dedicated ribosomes and their use |
US5149792A (en) * | 1989-12-19 | 1992-09-22 | Amgen Inc. | Platelet-derived growth factor B chain analogs |
-
2000
- 2000-06-22 FR FR0008002A patent/FR2810675B1/fr not_active Expired - Fee Related
-
2001
- 2001-06-21 MX MXPA02012880A patent/MXPA02012880A/es unknown
- 2001-06-21 CA CA002413612A patent/CA2413612A1/fr not_active Abandoned
- 2001-06-21 CN CN01812988.9A patent/CN1443242A/zh active Pending
- 2001-06-21 WO PCT/FR2001/001952 patent/WO2001098453A2/fr not_active Application Discontinuation
- 2001-06-21 AU AU69224/01A patent/AU6922401A/en not_active Abandoned
- 2001-06-21 JP JP2002504602A patent/JP2004500875A/ja active Pending
- 2001-06-21 EP EP01947565A patent/EP1315822A2/fr not_active Withdrawn
- 2001-06-21 BR BR0111907-9A patent/BR0111907A/pt not_active IP Right Cessation
- 2001-06-21 US US10/311,976 patent/US20040260060A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4738921A (en) * | 1984-09-27 | 1988-04-19 | Eli Lilly And Company | Derivative of the tryptophan operon for expression of fused gene products |
WO1998045427A2 (fr) * | 1997-04-04 | 1998-10-15 | Genentech, Inc. | Molecules agonistes du facteur de croissance de l'insulinoide |
WO1999007735A2 (fr) * | 1997-08-07 | 1999-02-18 | Creative Peptides Sweden Ab | Expression recombinee du peptide c de l'insuline |
Non-Patent Citations (3)
Title |
---|
MERINO E. ET AL.: "trp RNA-Binding Attenuation Protein (TRAP)-trp Leader RNA Interactions Mediate Translational as well as Transcriptional Regulation of the Bacillus subtilis trp Operon", JOURNAL OF BACTERIOLOGY, vol. 177, no. 22, November 1995 (1995-11-01), pages 6362 - 6370, XP002163024 * |
SAMBROOK J. ET AL.: "Molecular Cloning A Laboratory Manual", 1987, MOLECULAR CLONING A LABORATORY MANUAL, NEW YORK,COLD SPRING HARBOUR PRESS,US, XP002191819 * |
WILSON ET AL: "Increased Protein Expression Through Improved Ribosome-Binding Sites Obtained by Library Mutagenesis", BIOTECHNIQUES, vol. 17, no. 5, 1994, pages 944 - 953, XP002161835, ISSN: 0736-6205 * |
Also Published As
Publication number | Publication date |
---|---|
FR2810675B1 (fr) | 2002-09-27 |
MXPA02012880A (es) | 2003-05-14 |
CN1443242A (zh) | 2003-09-17 |
EP1315822A2 (fr) | 2003-06-04 |
AU6922401A (en) | 2002-01-02 |
FR2810675A1 (fr) | 2001-12-28 |
BR0111907A (pt) | 2003-12-30 |
US20040260060A1 (en) | 2004-12-23 |
JP2004500875A (ja) | 2004-01-15 |
CA2413612A1 (fr) | 2001-12-27 |
WO2001098453A2 (fr) | 2001-12-27 |
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